1OXW


Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like

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cd07199: Pat17_PNPLA8_PNPLA9_like 
Click on image for an interactive view with Cn3D
Patatin-like phospholipase; includes PNPLA8, PNPLA9, and Pat17
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132838
View PSSM: cd07199
Aligned: 92 rows
Threshold Bit Score: 111.269
Threshold Setting Gi: 125545017
Created: 7-Nov-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitenucleophile
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               ## #                                      #                               
1OXW_A         18 VLSIDGGGIRG--IIPATILEFLEGqlqexdnnadarladyFDVIGGTSTGGLLTAXIStpnen--nrpfaAAKEIVPFY 93   heartleaf nig...
YP_302831       5 VLSVDGGGVRG--IVAATILQEIEKrinkp-------lckvFDLVSGSSVGSLICGALCvknad--gtprySACDLLELI 73   Ehrlichia can...
3861146        14 LALLEGGGVKG--EIHLEKLKVIEEitgkp-------tckvFDFTGGTSVGGLILILLNlpdsdnpgkplfSAEQAQTLF 84   Rickettsia pr...
YP_247427      41 ILSLSGGGVKG--IAELVVLAEIEErtgks-------iselFPIISGTSVGGLIAGLLTipkeqgsniakySAKDALKIF 111  Rickettsia fe...
EEA46492      619 VLALDGGGSKG--IFQAMVLDAVYKkitpfap--gipfrelFDLIIGTSIGSAIGAAIGrm--------nfSPIDARRLI 686  Florida lancelet
4678298       100 ILSIDGGGMRG--ILPGKALAYLEHalksksgdpnariadyFDVAAGSGIGGIYTAMLFgsrdg--nrpifKADDTWQFL 175  thale cress
CAO46768       60 ILSIDGGGATDg-ILAARSLAHLEAslrhksgnpnarisdyFDVVAGSGAGGILAALLFtrgkd--ggplfSADEALRFL 136  wine grape
NP_001049586   77 VLCVDGGGGGLraLLSGRALAHLEAalrrasgdpdarvadyFDLVAGTGAGGVFAAMLFsthsr--gaplfHADDTWRLV 154  Japanese rice
XP_001754698   24 VLSLDGGGMHG--IIPARLLAHLEEllkqksgdqdariadyFDVVTGTSVGGLIATMLFtgden--srplfRAEEAWRLM 99   Physcomitrell...
XP_001773268   32 ILSLDGGGMRG--LIAARILSHLENilqekvge-kvklcdyFDLLAGTSTGAVLATMLVtpdan--gnptfTAEGCCEFY 106  Physcomitrell...
Feature 1                                                                                         
1OXW_A         94 FEHGPQIFNpsgqilgpkydgkylxq------------------------------------------------------ 119  heartleaf nig...
YP_302831      74 LMYAGKIFCnstvrnalslvfgpkysdkn--------------------------------------------------- 102  Ehrlichia can...
3861146        85 ERMVHNIFPegltfrklwscnglfsykfsp-------------------------------------------------- 114  Rickettsia pr...
YP_247427     112 TDAAPKIFEhhwydgikqifthkhsqgp---------------------------------------------------- 139  Rickettsia fe...
EEA46492      687 EDSVSTIFVksgwrrfsgttlavlsgtiysnknlqklledgfgdttlsgapvkvgsraslsstdslsqsqtsetlrprsl 766  Florida lancelet
4678298       176 TRNAKGLYGgagilkrvlrtgsgccsgta--------------------------------------------------- 204  thale cress
CAO46768      137 VENRRRIFRappagvlrrmfrpakae------------------------------------------------------ 162  wine grape
NP_001049586  155 ADHAPRLFRkavggstslfcrpkkrplaapt------------------------------------------------- 185  Japanese rice
XP_001754698  100 AERGREVFKkvsrssvwsklrwkssktswfsaks---------------------------------------------- 133  Physcomitrell...
XP_001773268  107 KKNGRLIFQhrwydpfhgsvrqlyrpkysgrr------------------------------------------------ 138  Physcomitrell...
Feature 1                                                                                         
1OXW_A        120 -------------------------------------------------------vlqeklgetrvhqaltEVVISSFDI 144  heartleaf nig...
YP_302831     103 ----------------------------------------------------lnavlqeifgdvtikdliaDFIVPSYDL 130  Ehrlichia can...
3861146       115 --------------------------------------------------dplvkllkkyckdytlkdligEVIVTGYDL 144  Rickettsia pr...
YP_247427     140 ----------------------------------------------------lkeileqhlaglrlddttsRLIIPVTDL 167  Rickettsia fe...
EEA46492      767 rsvskrlsmsvdmlqelkeecehtlfqhtrrrpslvvkndhkmfqhvgrrssivieeeeeeftpptdlvlpVVACCVYDM 846  Florida lancelet
4678298       205 ---------------------------------------------------klkkvmkesfseltlkdtlkPVLIPCYDL 233  thale cress
CAO46768      163 ------------------------------------------------------kvfqrafgeatlkdtlkSVLITCYDL 188  wine grape
NP_001049586  186 -------------------------------------------------aaldaamktafgeeltlrdtikPVLISCYDL 216  Japanese rice
XP_001754698  134 -----------------------------------------------leqvfkeylmrkdgsaltlrdtikPVLIPCYDL 166  Physcomitrell...
XP_001773268  139 ------------------------------------------------fedllkkytfidgkfltlldtlkPLVVTSFDI 170  Physcomitrell...
Feature 1                                                                       #                 
1OXW_A        145 k--TNKPVIFTKSnlanspelDAKXYDISYSTAAAPTYFPPhyfvtnts---ngdeyefNLVDGAVATvADPALLSIsva 219  heartleaf nig...
YP_302831     131 c--SNQTIMFRSWidk---ysDIKVCDVTRAAVAAPTYFTPkkmiv--------dnkkqLLVDSAIVC-NNPVIAAYsaa 196  Ehrlichia can...
3861146       145 nnqQNPLITFSTIearksqdnNYYLSDIIQGITAAPGYFPShhfsni------tntkvhTIIDGGIYA-NDPTLQTWqll 217  Rickettsia pr...
YP_247427     168 askDKEVKIFDSHdsy---spHIRVQDVLLATTAAPTYFKPvtnqehikgyenqedalyAYADGGLGA-NRPAYEALkil 243  Rickettsia fe...
EEA46492      847 d--RVKTVTLSGHd------cSLKAREAVLASTAAPTYFPSvqlly--------kgekrQFCDGAIGA-NCPAGEGKkla 909  Florida lancelet
4678298       234 k--SSGPFLFSRAdaletdgyDFRLSEVCRATWAEPGVFEPvemksv------dgqtkcVAVGGGLAM-SNPTAAAIthv 304  thale cress
CAO46768      189 s--TRAPFLFSRAdaleidgyDFKMKDVCVATSSDPTVAGGvelrsv------drgtriMAVDGRIAM-NNPTAAAIthv 259  wine grape
NP_001049586  217 k--SSAPLVFSRAdalesesyDFRLCEVGRAAWSEAGRFEPaevasv------dgatscAAVDGGPIM-GSPAAAAIthv 287  Japanese rice
XP_001754698  167 a--TAAPFLFSRAdalesqtwDFNLWEICRATTATPPFFPPacvtsv------dgntscTAIDGRVVM-HNPTAAAIthv 237  Physcomitrell...
XP_001773268  171 s--QATPFFFVRQaaqkdqsrNFRLWEVCRATAAAPTYFPPasvrsv------dgrvqgTLIDGGAVQ-NNPALVATtha 241  Physcomitrell...
Feature 1                                                                                         
1OXW_A        220 trlaqkdpafasirslnyKKXLLLSLGTGTTSEfdktytakeaat--wtavhwxlviqkXTDAASSYXTDYYLStafqal 297  heartleaf nig...
YP_302831     197 qv------------lypnEKICCLSVGCGTVSQsfsdlqnsl--------lywsrkilcVIIDAGLEAIDYEMArlv--- 253  Ehrlichia can...
3861146       218 ke-----------nkciiDNALYLSLKEENNADyktvccgggil-----ellknnmpnkILAATQQADEITAQRif---- 277  Rickettsia pr...
YP_247427     244 knghs-----reenakilDDTMVLSLNFDNDHKasssipk-------------igfdgvIGWLVKGKLVSRLMQsse--- 302  Rickettsia fe...
EEA46492      910 ke-----------vwpdrRIDTMLSVGCGGDATsgaskpns----------iatigaalLNFVVRTEQHWNELEk----- 963  Florida lancelet
4678298       305 lhnkq-----efpfvrgvEDLLVLSLGMGQLLDvsyeydriikw----kakhwarpaalISNDGAADTVDQAVAmafg-h 374  thale cress
CAO46768      260 lnnkq-----efpfcnsvGDLLVVSLGNGESDSgagnlss------------taselvrIAGEGASDMVDQAVSmafg-e 321  wine grape
NP_001049586  288 lhnkh-----efpfvrgvEDLLVLSIGGCSAGGtgaaadadlrrlrrwgpkdwarpiarIAADGAADLVDHAVArafg-q 361  Japanese rice
XP_001754698  238 lhnkr-----efpfvrgvEDLLVLSLGTGQIDQtymydtvrgwg-----tmqwvkslakIVLDGVADTVDHTISmaf--g 305  Physcomitrell...
XP_001773268  242 isnne-----efpyvnglEDVLILSIGAGQMDKkhdlqkarkwg-----mtkwvrpimdIMMDGTADTVDYQLAaayagn 311  Physcomitrell...
Feature 1                                                     
1OXW_A        298 dsKNNYLRVQENAltgttte---xddaseANXELLVQVGENLLK 338  heartleaf nightshade
YP_302831     254 kgEDSYCRISGDIvysacd----fgdasqENIKNLKKDAQKILQ 293  Ehrlichia canis str. Jake
3861146       278 gnKLIEIPTYIPEqhaem------snssiENLQALKEFAKKSIY 315  Rickettsia prowazekii
YP_247427     303 dsSTAEVRVGLPGedefve----vklpitKETSSLDNSSLKNIE 342  Rickettsia felis URRWXCal2
EEA46492      964 -dNDCFVRLDAPArvgige-----lplncKDVDKIKRYALSVQC 1001 Florida lancelet
4678298       375 crSSNYVRIQANGsnlgpwspnmdtdpsgSNVNMLMGVAEEMLK 418  thale cress
CAO46768      322 srATNYVRIQGNAmlgkr------hsnglKDSKQLVGMTEEMLE 359  wine grape
NP_001049586  362 crSSNYLRIQAKResmppcgpdgeydptpANVHALLAAADEMLK 405  Japanese rice
XP_001754698  306 ehRKHYLRIQVTYpgpglpn---ktetdvNDVNQLLKLSEDLLD 346  Physcomitrella patens subsp. patens
XP_001773268  312 ncSENYLRIQLSGlpnktsv---mdcatqKNIHDLITISDDLIK 352  Physcomitrella patens subsp. patens

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