1UXU,2ESD


Conserved Protein Domain Family
ALDH_F11_NP-GAPDH

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cd07082: ALDH_F11_NP-GAPDH 
Click on image for an interactive view with Cn3D
NADP+-dependent non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase and ALDH family 11
NADP+-dependent non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (NP-GAPDH, EC=1.2.1.9) catalyzes the irreversible oxidation of glyceraldehyde 3-phosphate to 3-phosphoglycerate generating NADPH for biosynthetic reactions. This CD also includes the Arabidopsis thaliana osmotic-stress-inducible ALDH family 11, ALDH11A3 and similar sequences. In autotrophic eukaryotes, NP-GAPDH generates NADPH for biosynthetic processes from photosynthetic glyceraldehyde-3-phosphate exported from the chloroplast and catalyzes one of the classic glycolytic bypass reactions unique to plants.
Statistics
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PSSM-Id: 143401
View PSSM: cd07082
Aligned: 29 rows
Threshold Bit Score: 619.587
Threshold Setting Gi: 94265647
Created: 2-Jan-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:NADP binding site [chemical binding site]
Evidence:
  • Structure:1UXU; Thermoproteus Tenax NP-GAPDH with cofactor NADP bound, defined using 3.5 A.
    View structure with Cn3D
  • Structure:2ESD; Streptococcus mutans NP-GAPDH thioacylenzyme intermediate with reduced cofactor nicotinamide moiety, defined using 3.5 A.
    View structure with Cn3D
  • Comment:The structure reveals that reduction of the cofactor upon acylation leads to an extensive motion of the nicotinamide moiety with a flip of the reduced pyridinium ring away from the active site.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
1UXU_A        18 VYPSYLaGEWGgsg-qeIEVKSP---------IDLATIAKVIsps---reEVERTLDVLFKRGRWSARdmPGTERLAVLR 84  Thermoproteus t...
NP_578484     23 EFKTYIaGKWIyge-kfKDIKSP---------INGEVIARVSils---reQIEEAISTTYEIGREKIRdyPGEKRISSFL 89  Pyrococcus furi...
CAJ74272      28 QREYLCnGTIKnwtgprQDVLSPicdm-hngtIQQRRIGSYPllteqqaiETLEAACQAYDHGRGTWPtmSIAERITHVK 106 Candidatus Kuen...
ZP_01688815   29 QREYLIdGELRqwqgpmNQVQSPvcie-tmegYQQKIIGSSPlltskeslEALTAAHKAYNKGQGFWPtlKVKERIQYME 107 Microscilla mar...
ZP_03022982   27 QDYYLInGELRrwdgplQEVVSPvqvk-seglLENERVGEAPllsveaalEALDVAVSAYHNGRGTWPtmSVGERIHCIT 105 Geobacter sp. M21
YP_001806600  29 QKEYLVnGKLEtwegetEESLSPiylk-teqeLVRQTIGTFPslnqeaalSTLEAAVTAYNHGRGKWPtmSVSQRIECVQ 107 Cyanothece sp. ...
YP_285282     29 QRAILInGEMRiwkgetRTVHSPvcvqgsdgsLSHVELGSVPvtgtveadAALAAAVTAYDHGRGAWPnlSVAERIACVG 108 Dechloromonas a...
YP_860779     17 QETYLSnGELIkwkgktDEVYSTiss---tddYKPTLLGTVPhmdektalEVLNSACDAYAKGQGDWPtmKVADRISCME 93  Gramella forset...
YP_001322685  25 QNKYLInGELCkwqgdfQNVYSPiyvr--tenNSSNLIGSYPlmdketalYALESAVNAYNNGQGEWPrmPIEQRIDCLK 102 Methanococcus v...
NP_251013     29 QRDYLVdGQLRhwdgplAPVRSPvfla-eadgERQVILGSTPlldadaalAALDAAVRAYDHGQGLWPnlRVAERIQHVE 107 Pseudomonas aer...
Feature 1                                                                                       #
1UXU_A        85 KAADIIERNLDVFAEVLVMNAGKpksAAVGEVKAAVDRLRLAELDLKKigGDYIPGDwtydtleTEGLVRREPLGVVAAI 164 Thermoproteus t...
NP_578484     90 KAAEMMKEAFDDFVRILILDAGKpksNAMGEVKATIERLEKTTFEFGRliGDYIPGDwseetleSEAIVKREPYGLILAI 169 Pyrococcus furi...
CAJ74272     107 GFTGRMKERRADVVKLLMWEIGKtlpDAQKEFDRTVDYITDTIDALKDldRVSSRFVie---qgVIGQIRRVPLGVVLCM 183 Candidatus Kuen...
ZP_01688815  108 VFVTEMQKQRDEVVKLLMWEIGKtlaDSTKEFDRTVTYIQDTINALKDldRRSSKFEie---sgVIAQIRRVPLGVTLCM 184 Microscilla mar...
ZP_03022982  106 RFSRRVQEKRDEVVKLLMWEIGKslkEAQGEFDRAIAYIRDTVEALKEldRVSSRFViq---qgIIGQIRRAPLGVVLCM 182 Geobacter sp. M21
YP_001806600 108 EFAYRMKEKRDTVVTLLMWEIGKsytDSCKEFDRTVNYIQDTIDALKNldRVSSRFEis---qgVIGQIRRAPLGIVLSM 184 Cyanothece sp. ...
YP_285282    109 DFTNQIVARRREIVNLIMWEIGKslaDSQKEFDRTIDYIRATVEELKRldNSNSRFEiv---dgTIAQIRRSPVGIALCM 185 Dechloromonas a...
YP_860779     94 DFVEQMKTKREKVVKYLMWEIGKslpDSQKEFDRTVEYIKDTIEDYKKldRDSAKFHek---dgVYAHIRRGPLGVVLCL 170 Gramella forset...
YP_001322685 103 NFVSLMKEQKNLVVNLLMWEIGKtlkESEKEFDRTIEYINDTIEALKDldRDASRITik---qgIIAQIKRAPLGVVLCM 179 Methanococcus v...
NP_251013    108 RFLARMREQREAVVKLLMWEIGKnlkDSEKEFDRTCDYIVDTIEALKEldRHSSRFEle---qgTLGQIRRVPLGVALCM 184 Pseudomonas aer...
Feature 1        ###                       # ##                             #    #            # #
1UXU_A       165 TPFNYPLFDAVNKITYSFIYGNAVVVKPSIsdPLPAAMAVKALLDaGFPPDAIALLNLPGKe-AEKIVADDrVAAVSFTG 243 Thermoproteus t...
NP_578484    170 SPFNYPLFISATKVIPALLSGNAVLLKPASadPLAPILFTRVLELsGLPKESFSLLTVPGGf-MDDIVRDKrIRAITFTG 248 Pyrococcus furi...
CAJ74272     184 GPFNYPLNETFTTLIPALIMGNVVIFKPPKlgVLLHHPLLEAFRD-SFPKGVVNTVYGKGQrvIGPIMKSGkVDVLAFIG 262 Candidatus Kuen...
ZP_01688815  185 GPYNYPLNETFTTLIPALIMGNTVVFKPAKygVLLIRPLLKAFCD-IFPKGVINIIYGRGRdtVGALMETGqVDAFAFIG 263 Microscilla mar...
ZP_03022982  183 GPYNFPLYETFTTLIPSLVMGNTILLKPPRfgILLFAPLLEAFRD-CFPPGVVNTVYGDGEevLPPLMASGrIDVLGFIG 261 Geobacter sp. M21
YP_001806600 185 GPANYPLNETFTTLIPALIMGNTVIVKAPRpgVLLNYPLLEAFKD-AFPPGVVNTIYGKGRtiTPPLMESGkIDALAFIG 263 Cyanothece sp. ...
YP_285282    186 GPYNYPMNETFSTLIPALIMGNVVLFKPPRfgVLLYYPMLEAFRS-AFPPGVINIVYGQGHvvVPHIMGSGqVNVLALIG 264 Dechloromonas a...
YP_860779    171 GPYNYPLNETFALLIPALIMGNTVVFKPAKhgVLLLSPLLEAFQS-SFPKGVVNVIYGRGRvvAAPIMQTGrIDVLALIG 249 Gramella forset...
YP_001322685 180 GPFNYPLNETFTTLIPALIMGNTVVFKPAKlgVLLLSPLLNAFKS-AFPKGVVNIVYGRGRnvIEPIMTSGkINVLAFIG 258 Methanococcus v...
NP_251013    185 GPYNYPLNETFTTLIPALIMGNTVVFKPAKfgVLLIRPLLEAFRD-SFPPGVINVIYGRGRetVSALMASGkVDVFAFIG 263 Pseudomonas aer...
Feature 1        #  #                                                                            
1UXU_A       244 STEVGERVVKVGg---VKQYVMELGGGDPAIVLEDaDLDLAADKIARGIYSYaGQRCDAIKLVLAErpvYGKLVEEVAKR 320 Thermoproteus t...
NP_578484    249 STEVGEHIIRTGg---IKAYHLELGGKDPAIVLNDaNIEESVEKIVKGIVTYsGQRCDAIRLILVEediYEQFRESLVAQ 325 Pyrococcus furi...
CAJ74272     263 SSRVADILKQQHpkphRLRCVLGLEAKNAGIVLKDaDLDLAVKECVLGSLSFnGQRCTALKMLFVHkeiADAFITKFSDA 342 Candidatus Kuen...
ZP_01688815  264 TNKGANDLKKMHpkphKLRSVLGLDAKNPGIVLPDaDMENAVAECVTGSLSYnGQRCTALKILFVHrsiVEEFLGKFTEA 343 Microscilla mar...
ZP_03022982  262 THKVADALRAIHprphRLRCVLGLDAKNPAIVLPDaDLELAVEECLSGALSFnGQRCTALKIIFVHksiAGEFIKGMAAG 341 Geobacter sp. M21
YP_001806600 264 GSRAANNLKKQHpkshRLRSILGLEAKNPGIVLPHaDLDLTVKECILGTLSYnGQRCTAIKIIFVHrsvIDTFLEKFTVA 343 Cyanothece sp. ...
YP_285282    265 SSEVADQLKKSHpktnRLRAILGLGAKNAAIIMPDaDIELTVRECITGALSFnGQRCTAIKMILVHqsiAETFLRRFCEE 344 Dechloromonas a...
YP_860779    250 NSKSAIALQDQHpyknRLRLVLGLEAKNPGIILPDaDLDLAIEECISGTLSFnGQRCTALKILYVHeevQEEFNKRFAKR 329 Gramella forset...
YP_001322685 259 SCNVANTLKKQHpyphLLKSVLGLEAKNPAIILSDaEIEHTIKECITGTLSFnGQRCTALKILFIHekiYDIFLEKFTSA 338 Methanococcus v...
NP_251013    264 THSGASDLKKLHprphRLRAALGLDAKNPGIVLPQvDLDNAVNEAVTGALSFnGQRCTALKILFVHesvLRPFLDKFSAR 343 Pseudomonas aer...
Feature 1                                                                                      # 
1UXU_A       321 LSSLrVGDPRDPt-VDVGPLISPSAVDEMMAAIEDAveKGGRVLAGGRRlg---pTYVQPTFVEAPadrvkdMVLYKREV 396 Thermoproteus t...
NP_578484    326 LSKIePKNPLEDenAIMGPLIDEDSANYIEEIYKDAieKGARPLLEFRRr----gNYVWPIVLEVNneilptLRAFNEDV 401 Pyrococcus furi...
CAJ74272     343 VDRLkSGMPWDEg-VQITPLPEPNKTTYLTELIEDAcsKGARVVNKDGGvv--rqTFIVPAVVFPVgp---gMRLFSEEQ 416 Candidatus Kuen...
ZP_01688815  344 VNHLkPGMPWDEg-VKLTPLPEPGKTDYLAGLVKDAerHGAQVVNAGYGggevneTYFHPAILYPVnd---qMRVYREEQ 419 Microscilla mar...
ZP_03022982  342 IAALpCGVPWQEg-VAITALPEPEKPEYLEGLLMDAeaHGARVVNEGGGar--sgSYFFPALLYPVtp---kMRIYQEEQ 415 Geobacter sp. M21
YP_001806600 344 VSELkFGMPWEDn-VFLTPIAEPGKPNYLQELVEDAknHGAEVINEGGGmi--neTFFYPAIVYPVnd---kMKIYSVEQ 417 Cyanothece sp. ...
YP_285282    345 VGKLaIGMPWEAg-VTLTPLPEMAMVTYMNECIADAlsKGAKVINPGGGtt--veTLFYPAVIFPVse---gMKLYREEQ 418 Dechloromonas a...
YP_860779    330 VDELkFGNPWNEn-VMLTPLPEPDKPKYIQELIDDAqkKGAKILNERGGat--seNYIFPAVLYPVte---dMQVYKEEQ 403 Gramella forset...
YP_001322685 339 VDNLnLGLPWDKn-VDLTPLPEKDKPMLLQEILEDAikKGAIICNKNGGiv--neTSFFPVVLSNVne---tMRIYHEEQ 412 Methanococcus v...
NP_251013    344 LASLkPGMPWEPg-VALTPLPEPGKVDYLEGLLNDAlaKGARVVNAGGGeh--rqSFFYPALLSPVsp---dMRLYHEEQ 417 Pseudomonas aer...
Feature 1                                                                                        
1UXU_A       397 FAPVALAVEVKdLDQAIELANGRPYGLDAAVFGRdVVKIRRAVRLL--EVGAIYINDMprHGIGYYPFGGRKKSGVFREG 474 Thermoproteus t...
NP_578484    402 FGPLALLIKVKdEDEAIEIANSSRFGLDAAVFTTcEKRARKVARKL--EVGSVFINEYprHGIGYYPFGGMKDSGIGREG 479 Pyrococcus furi...
CAJ74272     417 FGPVVPIATFSnIEEPMNYVIQSDYGQQVSLFGSnTNLIGEIIDTMvnQVCRVNINSQcqRGPDTFPFTGRKDSAEGTLS 496 Candidatus Kuen...
ZP_01688815  420 FGPVVPVVAYDnIEEVIAYVVSSNFGQQASIFGRdPQKIAELIDELyhQVSRININAQcqRGPDVFPFNGRKDSAEGTLS 499 Microscilla mar...
ZP_03022982  416 FGPVVPVVVYEdIKETIDYVVNSNYGQQASIFGQdDELLAQLIDALvnQVCRININSKcqRSPDSFPFNGRKNSAEGTQS 495 Geobacter sp. M21
YP_001806600 418 FGPVVPIVPFEdIETPINYLVNSDYGQQVSIFGQnTEAIAQLVDPLvnQVCRVNLNSQcqRGPDTFPFTGRKDSAEGTLS 497 Cyanothece sp. ...
YP_285282    419 FGPIIPVATFEdIETPLEYVITSDHGQQVSIFGSdPGQIASLVDTLvnQVCRVNINCQcqRGPDVFPFVGRRDSAEGTLS 498 Dechloromonas a...
YP_860779    404 FGPVIPIKPFNsIEKLLDEISDSNYGQQVSLFGTgIDKIAPLIDTLvnLVCRVNLNSScqRGPDVFPFTGRKDSAVGTLS 483 Gramella forset...
YP_001322685 413 FGPIIPIVKFNdIQEPLKYVINSNFGQQASIFGNdAIQISKLIDALvnQVCRVNINSQcqRGPDIFPFTGRKDSAEGTLS 492 Methanococcus v...
NP_251013    418 FGPLVPVVPYReLDEVIDYVLRSDYGQQLSLFGNdPAQIGRLVDAFanQVGRINVNAQcqRGPDSYPFNGRKNSAEGTLS 497 Pseudomonas aer...
Feature 1                         
1UXU_A       475 IGYAVEAVTAYKTIVFN 491 Thermoproteus tenax
NP_578484    480 IGYSLEMLTTTKTIVHN 496 Pyrococcus furiosus DSM 3638
CAJ74272     497 VSDALRVFTIRTLVAAK 513 Candidatus Kuenenia stuttgartiensis
ZP_01688815  500 VSDALRVFSIRAIVATK 516 Microscilla marina ATCC 23134
ZP_03022982  496 VADALRVFSIRIVVAAR 512 Geobacter sp. M21
YP_001806600 498 VHDALRAFSIRTLVAAK 514 Cyanothece sp. ATCC 51142
YP_285282    499 VHDALRAFSIRTMVAAK 515 Dechloromonas aromatica RCB
YP_860779    484 VHDALRSFSIRTFVASK 500 Gramella forsetii KT0803
YP_001322685 493 VSDALRVFSIRTMVAVK 509 Methanococcus vannielii SB
NP_251013    498 VHDALRVFSIRTLVATR 514 Pseudomonas aeruginosa PAO1

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