Conserved Protein Domain Family
ALDH_Acyl-CoA-Red_LuxC

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cd07080: ALDH_Acyl-CoA-Red_LuxC 
Acyl-CoA reductase LuxC
Acyl-CoA reductase, LuxC, (EC=1.2.1.50) is the fatty acid reductase enzyme responsible for synthesis of the aldehyde substrate for the luminescent reaction catalyzed by luciferase. The fatty acid reductase, a luminescence-specific, multienzyme complex (LuxCDE), reduces myristic acid to generate the long chain fatty aldehyde required for the luciferase-catalyzed reaction resulting in the emission of blue-green light. Mutational studies of conserved cysteines of LuxC revealed that the cysteine which aligns with the catalytic cysteine conserved throughout the ALDH superfamily is the LuxC acylation site. This CD is composed of mainly bacterial sequences but also includes a few archaeal sequences similar to the Methanospirillum hungateiacyl acyl-CoA reductase RfbN.
Statistics
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PSSM-Id: 143399
View PSSM: cd07080
Aligned: 28 rows
Threshold Bit Score: 357.358
Threshold Setting Gi: 81654098
Created: 11-Dec-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative catalytic cysteine [active site]
Evidence:
  • Comment:this cysteine is the site of acylation of the Lux-specific fatty acyl-CoA reductase and aligns with to the catalytic cysteine of NAD(P)+-dependent, aldehyde dehydrogenases.
  • Citation:PMID 9128139

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
P08639        29 KVKIDSFNNLHLTQmv-nHDYLNDLNINNIINFLYTTGQRWKSEEYSrrrayirslitylgyspqmakleanwiAMILCS 107 Vibrio harveyi
YP_001396403  15 EISFNNFSHVNDILks-nLKLLNSMPTQVIVLILDEYSKRLSRDKKLlkiq-------------------gvsyLSFYFR 74  Clostridium klu...
YP_504557     16 ERYVESFTELIQELee-nRRALFECPDEIIIHLFAHFGKEIIKDSSLayip-------------------glsyLSLWLR 75  Methanospirillu...
YP_001404904  13 TCTCQDFEDIAGSVns-gRINLAAMPLEGIIRILDALGKKILKNPEVnsvp-------------------gvsyISLWLR 72  Candidatus Meth...
ZP_02421838   28 TLGNCRLSAKQVMLa---AEKISTDIENGAFDDMLSALDVENVSYYKq-------------------------lILACLS 79  Eubacterium sir...
Q7MZU4        12 LEPASVLAELKGNLa---ETLACPPSVQQVLACAERFVDDLDNVAVKlaldt-----------------aaltaLRHFCH 71  Photorhabdus lu...
YP_606378     12 LTLDQALVRLTGELa---HTLSHPCGIETVLACAGRFAEKLLDGEFLaelda-----------------alreqLRAFCQ 71  Pseudomonas ent...
Q7NQ23         8 LRADLELDAALAALrgrlPDALAAEPDVDAVLSCAERFADALAGAERhpllde----------------pgraqLAAFCR 71  Chromobacterium...
Q2WLE3        15 PKKLQSLDAIIEELnk-nRETIYSYPSEAMIEIINEYSKILCSDRKFlsye-------------------gvpfLVLWLK 74  Clostridium bei...
Q97GS8        25 TSGKGDVEKELERLkk-nKNKVHDIKTEETIDLLDRCAKLWLDDNYSkkhietlaqitn---qsfelvnyefksMMQMLL 100 Clostridium ace...
Feature 1                                                                                        
P08639       108 KSALYDIIDTELg-sTHIQDEWLp--QGECYVRAFp--kGRTMHLLAGNVPLSgVTSILRGILTRNQCIVRMSASd-pFT 181 Vibrio harveyi
YP_001396403  75 KSNIEKLIKTSLk-dEKYLDEFVs-iGNDKYVKAQa--rGISCHWIAGNVSTLaLYSIFQSLIARNSNLVKVPEKs-mTL 149 Clostridium klu...
YP_504557     76 KENLHAIYLANFg-iKPGIRDSIq-vDSRSEMIHVp--rGVVCHWIAGNIPLLgFFSLVLATLGKNASILKIHPSv-yKY 150 Methanospirillu...
YP_001404904  73 RENLDRICQVNYt-dNRFADRFCqqaGSHSRMMAQp--rGIVCHWIAANMPTLgFFSIVQAFLSKNGSMAKIPKEy-vPL 148 Candidatus Meth...
ZP_02421838   80 RENLEYRLKTELgdsDGFIGPPN---GITPKIKIQtkplGVLFHIAAGNADGLpVTSVAEGLLAGNINILKLPSAd-kGL 155 Eubacterium sir...
Q7MZU4        72 RDSLEAKLRHELgeqPFSLRRFD---YTQPRFETWqp-lGLVVHITPANAPLLpFMAVLESLLVGNVNWLRPSSSdhgLN 147 Photorhabdus lu...
YP_606378     72 PDVLRAKLENELgvnPFSLRRRD---YRVPRFESWrp-lGLILHITPGNAPLLpFFAVLESLLVGNVNWLRPSGRd-qGL 146 Pseudomonas ent...
Q7NQ23        72 REALQAKLERELgerPFSLRRPD---YREPRFEAWrp-lGLVLHVTPSNAALLpFMAALEGLLAGNVNWLRPSSSdqgLS 147 Chromobacterium...
Q2WLE3        75 KDNIKKLLHQDLk-kQEFLDDFIe-iADNKFMKAQp--rGMVCHFMAGNVPTLpIYYLVQAIICRNVNLCRIPIGs-iDI 149 Clostridium bei...
Q97GS8       101 RENIQNTIKRELg-eVDILDSWHk--TNYGYEHRQp--rGIIFHNISGNAFVViPMSISMGLLSKNCNLVKVSKDe-pYF 174 Clostridium ace...
Feature 1                                                                                        
P08639       182 AHALAMSFIDVDpnh--------piSRSISVLYWPhasdttlaeELLSHm---DAVVAWGGRDAIDWAVKHsp-shIDVL 249 Vibrio harveyi
YP_001396403 150 VLRLLKLLDDIQvvyeekiydskdlLKNICLVYFPrede-slnkSMSIMa---DARIVWGGEDAVDYITSLpkkttCKDV 225 Clostridium klu...
YP_504557    151 LNPLLQKLEEVTieidrvsysgkviTLSVSLVSFPgkne-imsrDFSLSa---DCRVIYGSHEAVQAISCLphqvhADTI 226 Methanospirillu...
YP_001404904 149 ILSLLRELQGIIveventkctgetlLKAIAIVVFEgrsa-diseKFSRSa---DCRIVFGGSEAIHAITRLpcrdhCETI 224 Candidatus Meth...
ZP_02421838  156 SIRIIKRLCKYEp------------LLSRYIYVFDtpss--dvrTIQTLagfaDGIVVWGGTEAVKAVRSLap-pgVKLI 220 Eubacterium sir...
Q7MZU4       148 AQILAEFLRHDLs-----------gTLAPYISVLPlpsd--qlsELLACa---DAVSAWGSNSALTAIRSQlr-pgCRWI 210 Photorhabdus lu...
YP_606378    147 NTRLLQAFLQCDrs----------gELANHVAVLPvaka--elsKLMPYa---DGVSAWGGNEALKTIRDQlp-dgCRWI 210 Pseudomonas ent...
Q7NQ23       148 SRLLAELLRHDAs-----------gRLAARVAVLPapsd--alpRLMADa---DGVSAWGGDAALSAIRARlp-agCRWI 210 Chromobacterium...
Q2WLE3       150 VIELLKPLKDIVinfrgekyygstlLKSISIINFSsndl-klntEISQVa---DVRVICGGEEAVKTLSVLpkkttCKDV 225 Clostridium bei...
Q97GS8       175 AYAFYKSLCELDet----------vKDRLSVLYFDsskt-eiydTIVKNs---DGVIHWGGEYSSRIIGEMcakyqKHMI 240 Clostridium ace...
Feature 1                                               #                                        
P08639       250 KFGPKKSFTVLDHPa----dLEEAASGVAHDICFYDQNACFSTQNIYFSGDK------YEEFKLKLVeKLNLYQEvlpks 319 Vibrio harveyi
YP_001396403 226 VFGPKYSFAVFDREviegdkCFSYMDKLVMDIVLFQQKACSSPQVLFVEKGSv----pLKDVIEMLKkSFEKVGKrynds 301 Clostridium klu...
YP_504557    227 VYGPKYSFAVFDKEavespeFSSMLKGLAQDIVLYNQAACSSPHTIFFEKSKm----lLHDIAEQLKsAFQTLPErmfyp 302 Methanospirillu...
YP_001404904 225 VFGPKYSFAAFDREytesegFKKSLDLFARDIAVFNQMACSSPHVLFLEKSRf----sMETIAALLAeAFERLPPalrlq 300 Candidatus Meth...
ZP_02421838  221 EWGHKLGFCYISDYa----nHTDEFSALAEHIASTKQLLCSSCQTVFIDTDDkt---eLTLFCEMFLpYLEAAVGkhrnt 293 Eubacterium sir...
Q7MZU4       211 DWGHRISFAYFDPMa----aSDADYDALADDVCRFDQQACSSPQCLLVDSADen---vLREVVSAVAaALARRATiwpal 283 Photorhabdus lu...
YP_606378    211 PWGHKVSFAWLVPEa----vDECALDALADEVCRLDQQACSSPQVVFIDSDDad---tLRALGGQLAaAMQRRHAhwpal 283 Pseudomonas ent...
Q7NQ23       211 DWGHRVSFAYLDPAa----aADEALDALADDICRFDQQACSSPQALLVDSDDpa---aIHEMARRLAaALARRAPlwpal 283 Chromobacterium...
Q2WLE3       226 IFGPKYSFAVFEKSameslkITKTFDNFAKDIISFDQRACSSPQVLFIEKSTvgineaAQMLAQALEkSLKRYPKveinq 305 Clostridium bei...
Q97GS8       241 MHGAKISFEVVDKCd----dLNKVSEEIAKDIICWEQKACLSPRVVFLNDKId-----VDEFSHCLSaSLRKITEvipka 311 Clostridium ace...
Feature 1                                                                                        
P08639       320 kq----sfddeaLFSMTRLECQFSGLkvi--------sepENNWMIIESEPGvey--nhpLSRCVYVHk--iNKVDDVVQ 383 Vibrio harveyi
YP_001396403 302 l-----nestcaKIINERGVYSLDPDrdic------ssrgLEYTILINDQIVlee---pmGGRCIFVKe--iESIFDIEN 365 Clostridium klu...
YP_504557    303 ld---egiaisvINTRARYLLDSSKDill--------sddLKWTVCINDTISlee---pvQGRCVFVKe--vSDIDQVLP 366 Methanospirillu...
YP_001404904 301 ni----pvslsaKIINERAIYRLAEDkncv------apadLGWTILIGGRPGle----epVQGKCIFIke-vKALDDVIP 365 Candidatus Meth...
ZP_02421838  294 sig--trardtlISYSARLEQAIGIRqispd-----vfygKNCSLIIKDDFElel---spMMCNVLVKrlprKNIMRTLR 363 Eubacterium sir...
Q7MZU4       284 qtdiqeaaeissQMAFLKLDHTFAELrgsv-------eegNGWRVAWIQRQElap---spLYRTLQVRpaprAQLVEILL 353 Photorhabdus lu...
YP_606378    284 spsvqeaaqvttKLALAELDHAFVDGrgqv-------wrgQGWQIVLEHVMAlep---spLFRTILLRplprRAVMHALR 353 Pseudomonas ent...
Q7NQ23       284 tpdlqeaaeisaRMAFHKLDQAFADDqgqv-------lsgQGWRLAWSMEQElap---spLFRTVELRpaprAALARILR 353 Chromobacterium...
Q2WLE3       306 gaa--aniinkrGEYLISLEKDIICSk------------dLKYTILIDKEIIle----epVQHTTIFLke-iEDIFEVCK 366 Clostridium bei...
Q97GS8       312 yl----sewnsvKTIQDRQYCTLKYGmknerrfkvyssfnADYTVILSDGMPekedinkcFYRFIFVCp--ySSKSDVVN 385 Clostridium ace...
Feature 1                                                                          
P08639       384 YIek---hqTQTISFYPWESs--kKYRDAFAAKGVERIVESGMNNIFRAGGAHdaMRPLQRLVRFV 444 Vibrio harveyi
YP_001396403 366 LItr----rIQTIGHAISDKnklfKFADRVTALGVNRVVGVGTMNIYDSPWDG--NFMINELVTWC 425 Clostridium kluyveri DSM 555
YP_504557    367 LVtr----kVQTVALGMVDQvrkeRYVRELAYRGVDRVMAPGAMHEFTQPWDG--MLGAGRMVRWI 426 Methanospirillum hungatei JF-1
YP_001404904 366 QVnh----kIQAIAIGISDPekrkVFAQQVTARGADRIVIPGTMHDFILPWDG--IMTLNRLVRWV 425 Candidatus Methanoregula boon...
ZP_02421838  364 NAkg----fLQTAGLICGKAdr-aELTDLLLCAGVTRVTQAGNMSAYFFGESHdgEYPLSRYTRKI 424 Eubacterium siraeum DSM 15702
Q7MZU4       354 PWrp----yLQSCALIVKEKel-pMLSRQLLAAGVSRICQAGVMHDGYEGEPHdgVYALTRLARRV 414 Photorhabdus luminescens subs...
YP_606378    354 PWrt----rLQTCGLVSSAHef-aHLSQMLLAAGVDRVTSVQTMHDGYSGEPHdgVYALSMFARRV 414 Pseudomonas entomophila L48
Q7NQ23       354 PWrt----hLQSCGLIAPPArl-pELSRMLLAAGVSRVAPAGRMHDGYHGEPHdgVYALQRLSRRV 414 Chromobacterium violaceum
Q2WLE3       367 LItp----rIQTIGIASENNsriiNFANKASLHGVDRLVKVGLMNLYDSPWDG--ILFMSEIVKWT 426 Clostridium beijerincki NCIMB...
Q97GS8       386 YVsenlkdyLQTMGYSGNDE----EFIEKMTLLGVSIVTKPGKMSLHEPGTSHdgIHNLKEFTFLV 447 Clostridium acetobutylicum

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