2H5G,1VLU,1O20


Conserved Protein Domain Family
ALDH_F18-19_ProA-GPR

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cd07079: ALDH_F18-19_ProA-GPR 
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Gamma-glutamyl phosphate reductase (GPR), aldehyde dehydrogenase families 18 and 19
Gamma-glutamyl phosphate reductase (GPR), a L-proline biosynthetic pathway (PBP) enzyme that catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The glutamate route of the PBP involves two enzymatic steps catalyzed by gamma-glutamyl kinase (GK, EC 2.7.2.11) and GPR (EC 1.2.1.41). These enzymes are fused into the bifunctional enzyme, ProA or delta(1)-pyrroline-5-carboxylate synthetase (P5CS) in plants and animals, whereas they are separate enzymes in bacteria and yeast. In humans, the P5CS (ALDH18A1), an inner mitochondrial membrane enzyme, is essential to the de novo synthesis of the amino acids proline and arginine. Tomato (Lycopersicon esculentum) has both the prokaryotic-like polycistronic operons encoding GK and GPR (PRO1, ALDH19) and the full-length, bifunctional P5CS (PRO2, ALDH18B1).
Statistics
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PSSM-Id: 143398
View PSSM: cd07079
Aligned: 146 rows
Threshold Bit Score: 401.812
Threshold Setting Gi: 197784711
Created: 10-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:putative catalytic cysteine [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
2H5G_A        32 EQQGEXARSGGRXLatlEPEQRAEIIHHLADLLTDQRDEILLANKKDLeeaegr-----laapllKRLSLSTSKLNSLAI 106 human
XP_628638      9 GEIARRSRESGIQLsssTVEERNAILEHFAEEIYLNRNEILEANMKDMdnsnknn----isepmkMRLLLDESKLKACIS 84  Cryptosporidium...
Q18J39        13 EEKVTDAQTAALSLsnlSTARRNEALTAIADTIERNVDRILEANAADVadaekqvdageytqafvDRLTLSTSKIESIAE 92  Haloquadratum w...
NP_618968      6 ETKVVKAKKASIELssvSEEVKNRALEAMAESLDRERKAILEANSKDLeyaselkkvgkltqalvDRLKVTDSKIDGMIA 85  Methanosarcina ...
NP_488035     13 MKSAKRAFQASLKLgttKGIDRSRAVLAMAQALENAFDDILEANTLDLeasrema----vpelilDWLKLTPTRLEITVE 88  Nostoc sp. PCC ...
ZP_01624677   13 TNPIRRSYAASIELaktKGEHRSRALRAMAEAIRKRQNDILEANTLDLeasrelg----vsecvsEWLKLTPERLDTTIQ 88  Lyngbya sp. PCC...
NP_442236     11 VRVLDAAQGAFLALdsyGGNDRSRAVLAMAEALERSFAQILEANTLDLvvsrems----vadclcEWLKLTPERLQNTVT 86  Synechocystis s...
YP_001733846  14 TAALQRAHQASLELellKGSERHLGIEAIATALEANISVILEANTKDLeasrema----vpklilDWLKLTPERLQKTVR 89  Synechococcus s...
YP_001157531   6 DNILDGATRARAELptpGDERYDRYCEALADELSSGWADVLAANATDVaqgvrag----lgpelvDRLALGKRHLDALVD 81  Salinispora tro...
YP_002207471   3 DALLSRARAAFDAAppvGDPAYDQYCRFLGEELVAHWPQIARANAADVaaaqrlg----mpptlvERLRLTDVHRDRMVE 78  Streptomyces sv...
Feature 1                                                                                        
2H5G_A       107 GLRqiaassqDSVGRVLRRTRIa--kNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEaaHSNRIL 184 human
XP_628638     85 SVRdvm-nleDPLGKYTLSREIts-nGLKLYRRTCPIGVILVIFEARPEVAIQISSLTIKSGNAVILKGGKEaiNTNKAI 162 Cryptosporidium...
Q18J39        93 MVRsva-eqdDPLGETLTARELd--dDLNLYKVTVPIGVIGTVFESRPDALVQISALSLKSGNAVILKGGSEaeRSNRVL 169 Haloquadratum w...
NP_618968     86 GIMdvi-klkDPVGETLSTLELd--nDLILYQISCPIGLIGVIFESRPDVVPQVMSLCLKSGNATIFKGGSEarESNRTI 162 Methanosarcina ...
NP_488035     89 ILQrlg-eisDPLRRVRNADYQp--eDSQSYSQLMPLGVIGFIYEAFPDLGAIAAGLCIKTGNSIILKGSTEasHSNAAI 165 Nostoc sp. PCC ...
ZP_01624677   89 ILKrlg-elpDPIGRVIGASYQl--eQGQTYYQLIPIGVVGLVYEAFPELGAIAAGLCMKTANSLILLGGSEanYSNQVI 165 Lyngbya sp. PCC...
NP_442236     87 ILKrla-slpDPLQRVMASPYQf--nLAQTYCQLMPLGVVALVYESFPELAAIAAGFCLKTGNSLVLRSCGAssHSTAAI 163 Synechocystis s...
YP_001733846  90 LLRqlp-klpDPLQRVKNATYQi--sGYQTYSQRMPLGVICLIHEALPELGAIAAGLCLKTANSLVIRGCGDasHTNHLI 166 Synechococcus s...
YP_001157531  82 LIArtr--aaLPTVTAPTVGHRag-nGATVRRLPKPLGVVLMIFEARPTVTVEGALLPVAVGNVAILRGGTEiaATNQAL 158 Salinispora tro...
YP_002207471  79 LTAmt----rDELRKVREAEVPvsgaARHVHRVPRPLGVVLMVYEARPTVTVDGALLPVAVGNAVLLRGGKEtaATNAAV 154 Streptomyces sv...
Feature 1                                                                                        
2H5G_A       185 HLLTQEALsi------hGVKEAVQLVNtre--eveDLCRLdkxiDLIIPRGSSQLVRDIQKAakgIPVXGHSEGICHXYV 256 human
XP_628638    163 LNCIENAInqvkknyinINREIVQMVFshe--dvnKLLSFnqfiDLVIPRGSGELVHMIKKNt-cIPVLGHADGVCMLYV 239 Cryptosporidium...
Q18J39       170 YNCILEATp-------eVPSGWAQLIEare--dvqTLLEMddavDLIMPRGSSSFVQYVQDNt-sIPVLGHTEGVCHVYV 239 Haloquadratum w...
NP_618968    163 FEILVKAIest----egMPKGAFQLMEtre--eimSLLSLdayvDLLIPRGSNEFVKFIQDNt-kIPVLGHTSGICHIYV 235 Methanosarcina ...
NP_488035    166 VEALQHAIve-----vgLPSGCIELVTtehggslrDLITQdqyiNLVIPYGRSSLVQQVMRQa-tCPVLKSAMGNCYLYW 239 Nostoc sp. PCC ...
ZP_01624677  166 ADTLQSAIee-----egMPIGSLEVFPneqeapiqDLVTQdqyiNLIIPYGRPHLVHQVVAQs-tVPVLRSAMGNCYLYW 239 Lyngbya sp. PCC...
NP_442236    164 CEILREGLld-----adLPVDSVSHIPsetspnvqDLVGNasqlNLVIPYGRPSFVEQISQQc-tPPVLKAAMGNCYLYW 237 Synechocystis s...
YP_001733846 167 AEIMQTALad-----qgLPAGCLESLSseegctvqQLVAQdrylNLIIPYGRPNMIQQVVQHa-tTPVLRTTVGNCYLYL 240 Synechococcus s...
YP_001157531 159 AVVVGRALkd-----aqLPMGTVQVVTdpdrrlvrALLKRrdkiDGLIPRGSPSLIDYCLTSs-tIPVIASGGGVNHLYV 232 Salinispora tro...
YP_002207471 155 ERTVHAALtr-----agLPTGLVTVLDdpqrrllrALLKRpdevDVLIPRGSPALVEHCRTSa--IPLIAGGGGVNHLYV 227 Streptomyces sv...
Feature 1                               #                                                        
2H5G_A       257 DSEaSVDKVTRLVRDSKCEYPAACNALETLLIHRDLlrtplFDQIIDXLRv--eQVKIHAGpkfasyltfs--------p 326 human
XP_628638    240 HVDaNISKGVEIVIDGKLDYPAACNSLETLLIHKDKls-efLPALLLRLKeqgkHIKFYSDkeclkylegend----vce 314 Cryptosporidium...
Q18J39       240 DSEaDLEMATAVAYDAKVQYPAVCNAVETLLVHESVap-sfLSQMMKQYRd--aGVEIRGDertqsivdtesniseeiia 316 Haloquadratum w...
NP_618968    236 DEFaDPDTAWKVCFDAKVQYPAVCNAIETLLVNRKIae-afLPKMAEMYIk--aGVELRCDkdsyillaekgls--plsk 310 Methanosarcina ...
NP_488035    240 SLNsSLDMVRWMILDSHESEPDPVNAIEKVLIHRQAmp-ssLAVLWNSLKe--kGFEIKGDgelveafpql-------ql 309 Nostoc sp. PCC ...
ZP_01624677  240 SATaNVDMVRWMIFDSHQSKPDPVNAIEKVLIDPNQkg-ssLVRLWNNLRe--eGFLLKGDellvkqfpel-------aq 309 Lyngbya sp. PCC...
NP_442236    238 SSKgDLEMVRQMIIDSHVGHPDPVNAIEKVLVSPGQnp-apLVRLLNNLQa--kGFKLRGDaelceqfpdhl------tl 308 Synechocystis s...
YP_001733846 241 APSgDLELASWVIQDSHNYEPDAVNAIEKVLVCPEQkt-shLISLFKSLQe--qDFELRGDaslveefpehl-----api 312 Synechococcus s...
YP_001157531 233 DRSaDLELAARIALDSKLGEPTACNTLELVLAHEDVae-elTRALVRAGAelaePWLLRVDprltaavppeap----vae 307 Salinispora tro...
YP_002207471 228 HRSaDLDLAVRATLDSKLPEPAGCTSVETLLLDRAVar-dyLTALARGLAgregELTLHTApeldavvpctlk----tep 302 Streptomyces sv...
Feature 1                                                                                        
2H5G_A       327 sevkslrtEYGDLELCIEVVdnvQDAIDHIHKYGSsHTDVIVTEdeNTAEFFLQHVDSACVFWNASTRFSDGyrfGLGAE 406 human
XP_628638    315 liedlyhhEFSSNEMCVKSVssmDEAIAHINQYGShHTDVILTQdeKLAASFMNRVDSACVFHNCSSRFSDGyrfGFGAE 394 Cryptosporidium...
Q18J39       317 ateadwttEYGDRIVSIAVVdslTDAIDHINMYGSkHTESILTQddNRAEQFMRAVDAASVFHNASTRFADGfrfGLGAE 396 Haloquadratum w...
NP_618968    311 ateedwslEYNDLILSIKLVdtiKEAIAHINTFGShHTDGIITEnaSRRKEFIGLVDSSSVMVNASTRFADGyryGKGAE 390 Methanosarcina ...
NP_488035    310 akdnewgnAYLTKTVAFKLVdslDGAIAWINQYSSgHADSIATEsyQESRQFALGVNSAATYINTSPRFSRNpsrGDSVF 389 Nostoc sp. PCC ...
ZP_01624677  310 aeasewsqPYLNKTIAFRGVdslEEGIAWMNEYSSgHSNCLATEsySESRQFALDVNSALTFINASPRFSRHqnrGDAIF 389 Lyngbya sp. PCC...
NP_442236    309 akenewgkAYLDRTVAFRTTqnlKTAIAWINSHSSgHGDCIATDsyQESRQFSMGVDSALVYVNIPPYFCRNprhGESLF 388 Synechocystis s...
YP_001733846 313 gteeewqqAYLEKIVAFKRVpdlAAAITEINCNSGgHADCIVTEsyHESRQFSMGLDSALIYINASPCFSRHplgSDSVF 392 Synechococcus s...
YP_001157531 308 laaaddgrEFLERAVGIRPVaglDEAVAHIRRFGSqHTEGVVAAdpVSVEAFLERVDAATLVVNGSLRLHDGptmGLGPE 387 Salinispora tro...
YP_002207471 303 laahdlgrEFLDPIVAVVPVddeNAALDHVRRHGSgHTEGVIARdrHVIDAFRRRADAAMVVVNGSLRLHDGptlGLGTE 382 Streptomyces sv...
Feature 1                                     
2H5G_A       407 VGISTSRIHARGPVGLegLLTTKWLLRGK 435 human
XP_628638    395 IGISTTRIHARGPVGLegLLTYKYTLRGQ 423 Cryptosporidium parvum Iowa II
Q18J39       397 VGISTGKIHARGPVGLdgLTTYKYHLEGD 425 Haloquadratum walsbyi DSM 16790
NP_618968    391 VGISTNKIHSRGPVGMegLLIYKYILMGK 419 Methanosarcina acetivorans C2A
NP_488035    390 LGMSNQKGHRRGFISLetLTTLKHIIQGN 418 Nostoc sp. PCC 7120
ZP_01624677  390 LGMSNQVGYRRGLIGLdsLTTLKYIVQGN 418 Lyngbya sp. PCC 8106
NP_442236    389 LGVSSQKGQRRGLIGLeaFMTPKQIVQGE 417 Synechocystis sp. PCC 6803
YP_001733846 393 LGVSNQKGNRRGLIGLesFTTIKQVVQGD 421 Synechococcus sp. PCC 7002
YP_001157531 388 LSIATGRLHVRGPVSLsaLLTWSWVVDAG 416 Salinispora tropica CNB-440
YP_002207471 383 IAIATGRLHVRGPVTLgaLVTYSYVTDGA 411 Streptomyces sviceus ATCC 29083

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