3BEZ,3BF0


Conserved Protein Domain Family
S49_Sppa_N_C

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cd07023: S49_Sppa_N_C 
Click on image for an interactive view with Cn3D
Signal peptide peptidase A (SppA), a serine protease, has catalytic Ser-Lys dyad
Signal peptide peptidase A (SppA; Peptidase S49; Protease IV): SppA is found in all three domains of life and is involved in the cleavage of signal peptides after their removal from the precursor proteins by signal peptidases. This subfamily contains members with either a single domain (sometimes referred to as 36K type), such as sohB peptidase, protein C and archaeal signal peptide peptidase, or an amino-terminal domain in addition to the carboxyl-terminal protease domain that is conserved in all the S49 family members (sometimes referred to as 67K type), similar to E. coli and Arabidopsis thaliana SppA peptidases. Site-directed mutagenesis and sequence analysis have shown these SppAs to be serine proteases. The predicted active site serine for members in this family occurs in a transmembrane domain. Mutagenesis studies also suggest that the catalytic center comprises a Ser-Lys dyad and not the usual Ser-His-Asp catalytic triad found in the majority of serine proteases. Interestingly, the single membrane spanning E. coli SppA carries out catalysis using a Ser-Lys dyad with the serine located in the conserved carboxy-terminal protease domain and the lysine in the non-conserved amino-terminal domain.
Statistics
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PSSM-Id: 132934
View PSSM: cd07023
Aligned: 275 rows
Threshold Bit Score: 123.752
Threshold Setting Gi: 55379667
Created: 2-Dec-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:active site residues [active site]
Evidence:
  • Comment:The N- and C-terminal halves of SppA in E. coli are tandem repeats and the Ser/Lys dyad is arranged such that the nucleophile Ser is located in the C-terminal half whereas the general base Lys is located in the N-terminal half.
  • Structure:3BEZ: Escherichia coli Signal peptide peptidase A (SppA)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
3BEZ_A       302 SIGVVFANGaixdgee-----tqgnvgGDTTAAQIRDARLDpkvKAIVLRVNSPGGsvTASEVIRAELAAARaa-gKPVV 375 Escherichia coli
NP_968002     42 TILHLEMNGvi--------------lnGKKFIKNLKKYRDDdkvKAILISINSPGGsvGPSQELFAEIKRVRdeikKPVV 107 Bdellovibrio ba...
NP_275945     34 TIAIIPVHGaiaydta----gfsdgvdPDDIKELIAEANSDpsvKAIVLDINSPGGtpVASEELMDAINKSE----KPVV 105 Methanothermoba...
NP_247635     58 GDGIFPQQKk----------------dARYYINLLDDLEKDdsvKGVLLVVNSPGGevIASEKLARKVEELAk--kKPVV 119 Methanocaldococ...
NP_712392    289 VVAVLPLKGnihhdtigkgegktdgisYYSIKEALKELKKEssvKAVILEVDSPGGsaFVSELLYQEILKLQk--kKPVY 366 Leptospira inte...
YP_001322912  50 SDSGLLSTGtt---------------gVEDYIKWLDEVENNdniKAVIIKINSPGGevVASEKLSRKIKEVSe--kKPVV 112 Methanococcus v...
ZP_01871123   58 YVAVININKtit------------vdyIDKITKKMDALLKDkncKEFLLVFNTPGGspSASDEFNAYLKYVNk--kKRVN 123 Caminibacter me...
ZP_02192395  101 STPEDDLFSeyp-------------pdVYDYIDWLDEAEKNpdiKGVIIKINSPGGssIASTKLAEKIEEVSq--kKPVV 165 Methanococcus v...
AAU82198      21 ITSEESVFGlm--------------aaSTLIIKDIEEVENKrkiKTVIFEINSPGGtpFASKEIATCIENMD----KPAI 82  uncultured arch...
ZP_02016942   58 DTGRPSPLSrag------------gatADDVVEQIEAADEDedvEALVVELNTPGGevLPSDDIRRAAADFD----GPTL 121 Halorubrum lacu...
Feature 1                #                                                                       
3BEZ_A       376 VSXGgXAASGGYWISTPanyIVANPSTLTGSIGIFGVIttvensldsiGVHTDGVsts-plADVSitRALPpe-aQLXXQ 453 Escherichia coli
NP_968002    108 CVSTgVMASGAYYAAVAcdkIVVAPGALVGSIGVIMEFanleklydwaKISRYSItsgkfkDSGAeyRPMRed-eRSLFQ 186 Bdellovibrio ba...
NP_275945    106 SWISdSGTSGAYLAASAsdrIVASPSAWVGSIGVILDLtdlsqmyrqmGINKYAIkageykDMGAdyRMITde-eRQMLQ 184 Methanothermoba...
NP_247635    120 VYVEgLDASGAYMVSAPadyIVAEKHSIVGSIGVRMDLmhyyglmkklGINVTTIkagkykDIGSpfRPMTke-eKEYLQ 198 Methanocaldococ...
NP_712392    367 AYIQnISASGGYYLSCGaskIYASPYGIVGSIGSISIRfdlknlysklGITKDRVgfykyrDLLSeyGPIHpe-sKKLME 445 Leptospira inte...
YP_001322912 113 AYIEtIGASGAYMAASSsnyIVAEKQSLVGSIGVRMELlhyyglmeklGVNTTSItggkykNIGTpnRPMTee-eYKMLE 191 Methanococcus v...
ZP_01871123  124 VYVEsMAASGGYYIISAikpIVANKNAVVGSIGVIMPHfvlkkladkiGIEEDSLtigkykEPVSlfRKFSdkdkEYLKT 203 Caminibacter me...
ZP_02192395  166 AYIEaMGASAAYGAAAStgyIVAEKEALVGSIGVRMDIlhyyglmdkiGVNSTVIkggeykDIGSpyRPMTee-eQEMLQ 244 Methanococcus v...
AAU82198      83 AWVReYGASGAYWIASAcdeIVADELSTVGSIGAMSIRpdigelmkkfGIDVETLrtgiykGMGLp-YEKPteeeRELMK 161 uncultured arch...
ZP_02016942  122 AYATdTCASGGYWIASGcdeLWARDASLVGSIGVVGSRpnaagladklGISYEQFtageykDAGVplREIEed-eREYLQ 200 Halorubrum lacu...
Feature 1                                                                        
3BEZ_A       454 LSIENGYKRFITLVADARhstPEQIDKIAq---GHVWTGQDAKANGL--VDSLgdfdDAVAKAA 512 Escherichia coli
NP_968002    187 SMIDEVYAQFKGTVATERklkEEVVAEYAd---GRVFTGATAVKVGF--ADQEgyfdDAVKLAA 245 Bdellovibrio bacteriovorus HD100
NP_275945    185 SMVNEEYDYFIKTVAANRnlsVSYVKSLAe---GRIFTGRQAVRNRL--VDFTg--gKDYAVEV 241 Methanothermobacter thermautotr...
NP_247635    199 KMINETYMDFVKWVAEHRhlsINYTLKIAd---GKIYSGEDAKKVGL--VDEVg--tEEDALKK 255 Methanocaldococcus jannaschii D...
NP_712392    446 QEIKESEGLFYKRVSDSRkipISDLDKRFg--qGKVFTSSQFLKERM--IDSItd-fLSLLEDI 504 Leptospira interrogans serovar ...
YP_001322912 192 SIVDEMYFDFISMVAENRnmtINETLIAAd---GKIYNGLQAKNAGL--IDQVg--tEKDAIDM 248 Methanococcus vannielii SB
ZP_01871123  204 HLLLPTYENFLKTVAKDR---NISVDKLKqyadGKIYVASIVKGILVdkISTLidvkEEIKKRL 264 Caminibacter mediatlanticus TB-2
ZP_02192395  245 KIVNNSYVDFVSQIARDRnmtYEEANELAq---GKVYDGRDALQLGL--VDENg--dFDQAINV 301 Methanococcus voltae A3
AAU82198     162 KELDEIQDNFLNAVAKNRkldDAKMKELSt---AKVYLGREAKEMGL--IDHLg--gKELAIRI 218 uncultured archaeon GZfos11H11
ZP_02016942  201 GIIDGYYEQFVETVSEGRd-mDPEAIRETe---ARVYLGSDAAEIGL--VDELg--tEDDVEDR 256 Halorubrum lacusprofundi ATCC 4...

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