Conserved Protein Domain Family
S49_Sppa_36K_type

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cd07022: S49_Sppa_36K_type 
Signal peptide peptidase A (SppA) 36K type, a serine protease, has catalytic Ser-Lys dyad
Signal peptide peptidase A (SppA; Peptidase S49; Protease IV) 36K type: SppA is found in all three domains of life and is involved in the cleavage of signal peptides after their removal from the precursor proteins by signal peptidases. Members in this subfamily are all bacterial and include sohB peptidase and protein C. These are sometimes referred to as 36K type since they contain only one domain, unlike E. coli SppA that also contains an amino-terminal domain. Site-directed mutagenesis and sequence analysis have shown these SppAs to be serine proteases. The predicted active site serine for members in this family occurs in a transmembrane domain. Mutagenesis studies also suggest that the catalytic center comprises a Ser-Lys dyad and not the usual Ser-His-Asp catalytic triad found in the majority of serine proteases.
Statistics
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PSSM-Id: 132933
View PSSM: cd07022
Aligned: 61 rows
Threshold Bit Score: 153.102
Threshold Setting Gi: 3128372
Created: 2-Dec-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:active site residues [active site]
Evidence:
  • Comment:The N- and C-terminal halves of SppA in E. coli are tandem repeats and the Ser/Lys dyad is arranged such that the nucleophile Ser is located in the C-terminal half whereas the general base Lys is located in the N-terminal half.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
CAM75754      57 GIAIVPVVGTLvarsgylg-aasgltgYGDIADAIEAAATdpgvRAILLDVDSSggevGGLFDLVDHIQAIRsq-cgKPI 134 Magnetospirillu...
ZP_03310833   47 GVAVISITGVIsrkesw-----wadvgQDGIRQALDEARQdervRGILLSFNSPggvaAGVKELADYIASIDd----KPV 117 Desulfovibrio p...
YP_012083     71 GVAVIPVMGMLtprrswswgygyttgmLDQIRPAIASALAdrgvRAILLDVASPggtvAGMKELADYIGAERak-gtKPM 149 Desulfovibrio v...
ZP_02929088   62 NVAIIRIDGVIdkrlaaleaecfggvdLEVIDEALQQAEHdravTHVVLDIHSPgggvTGTPETAARIARLAq---tKEV 138 Verrucomicrobiu...
ZP_03128185   51 GIGIIPITGVLmkrpdifsrvllgaadMDEISEAINEAGDredvQAVLLDIDSPggtvTGTPELAAAVAALSr---aKYV 127 Chthoniobacter ...
ZP_02926737   19 NVAVIPIYGVLaknagllqqvcmgfcdINPIAHAVNQAAKakdvSTIVLDIASPggqvKGIRETASAVRAAAkt-rgKTV 97  Verrucomicrobiu...
ZP_02010689   62 GIAEIPVFGPIgkglgsfe-kscgatsTEDVANDITEALGrrdvSGILLNINSPggttTGVPELASAIARAAe---nKPV 137 Opitutaceae bac...
YP_966510     50 DVAVVNMRGTLtkrgs------wwttgMEGVRTTVQQALDdpavRAVLLAIDSPggtvDGTRELADWIAARVad-ggKPI 122 Desulfovibrio v...
ZP_00957487   93 NVALIEIEGVLmsrgfegvwsgcywpgYRDYVAAVRAANEdervDAILLRFDTPggyvAGCAEAAQALRNLNqsnggKPL 172 Oceanicaulis al...
YP_001877941  48 SSLVIDVIGPLygdgvpln-evlggtnYQSIVKEIKGAAGn--iDEVVFIFDSPggdvQGCHETAELIKSLP-----VET 119 Akkermansia muc...
Feature 1                 #                                                                      
CAM75754     135 WAVAdeAALSAAYAIACTadrIYVTQTGEVGSIGVVAVHRDesgadaqaGLAWTFVHag--aAKVDGNPHQPLSdsaRAG 212 Magnetospirillu...
ZP_03310833  118 AAYAdgLTASAAYWLASAtgrVYAPAMAQVGSVGVISEVRNisgfldkmGVSITYIAsg--kWKTAGNPVEKLTpeqTAY 195 Desulfovibrio p...
YP_012083    150 AAYAngLMASAAYMIGSAtgrVLAPATATVGSIGVISVYENwskwnekaGLSYAYLTag--qWKAVGNPDTPLAdneRAY 227 Desulfovibrio v...
ZP_02929088  139 HAFVncQACSAAYWLASQadvIAAAPSSCIGSIGVYMALLDetralemeGYTVNLIKag--kHKAMGASFQKLTdeeRAI 216 Verrucomicrobiu...
ZP_03128185  128 YAFSdgQMCSAAYWIASQadvIFSTPSARVGSIGVLLPMLDeteafkqeGLKVDLFAag--kYKSVGVPGVALTddqRTW 205 Chthoniobacter ...
ZP_02926737   98 YAFSdeNMASSAYYLGSQaneIYVTPSATVGSIGTYLAWLDptmklameGLKLEFFGag--qHKGMGLPGKPLSegdRVL 175 Verrucomicrobiu...
ZP_02010689  138 VAFTdsLMASAAYWLGSQadmIVASKSASVGSVGVYIPFLDssrraemmGLKVDVVKnkegtLKGMGIPGTSLTeaqREH 217 Opitutaceae bac...
YP_966510    123 ASYAdgTMCSAAYWIGGStgrVFAPAAALVGSVGVLWMHYDwsqyletnGIRATYITag--sRKAAGAPEAPLSdndKAY 200 Desulfovibrio v...
ZP_00957487  173 VGHAdeLCASAGMKLAAQcdgLFASDGAMVGSVGVRIGFFDfegalekwGERYHVYKsg--rLKDMGSPLRAPTdeeSAI 250 Oceanicaulis al...
YP_001877941 120 VAYVkgMCCSAAYYLASAcdqVVVTETALVGSIGTVISLWNa------kSEEILTITn---dDAVFKHPETPMNteaIAY 190 Akkermansia muc...
Feature 1                                                                                    
CAM75754     213 LQADVDALYGRFVALVARCRkqpPEAIRATe---AAVYRG--------DQAVAAGL-----ADKVgtlrVALADLG 272 Magnetospirillum gr...
ZP_03310833  196 FQERVDALHTVFKADVAQHMgisQDPAWTE----AQILFA--------QSAQQLGL-----VTAIv--rDEDQAIN 252 Desulfovibrio piger...
YP_012083    228 LQERLTALYRHFTDGVSASMgldAANLTTWa--dGKVFVA--------SEAPQGLVta-ivADRE----AAIATLA 288 Desulfovibrio vulga...
ZP_02929088  217 FQGQVDSIYADFKKAVTASRr--IADSTMQ----GQSFDG--------KTALKLGL-----VDVLt--sDSLEEYA 271 Verrucomicrobium sp...
ZP_03128185  206 LQSMIDEINGEFQAAVLARGr--AIDPAAMe---GQDFSG--------QKAFENSLtagvmLDRA----TVLTKMQ 264 Chthoniobacter flav...
ZP_02926737  176 LQATVDEFNGWFTSAVQGAR---PKVVNATm--qGQSFSG--------ERAVGVRL-----ADGIcdtwDEFIDLL 233 Verrucomicrobium sp...
ZP_02010689  218 LQQRADEIFSMFKGDIKSRRp--GVKDEALr---GQTLLA--------AGSRETGLid-diGDIQha-rASLAALI 278 Opitutaceae bacteri...
YP_966510    201 FQHLIDTAYTQFLDGVSAPMgldRANPAAWa--dGQVFRA--------TEALALGL-----VTAIv--pDLAGAVA 259 Desulfovibrio vulga...
ZP_00957487  251 YQAEVDHLADRFYVELALGRgldLEAVRESrgweARTFTAgdppppaeLDPLAVDL-----IDAVm---TEEAAFA 318 Oceanicaulis alexan...
YP_001877941 191 YRDLCNKIAGRFQEFVASGRp-gLSADAFS----GKVFVA--------EDAVSLGL-----IDDV----IPWVDFP 244 Akkermansia mucinip...

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