3BEZ,3BF0


Conserved Protein Domain Family
S49_SppA_67K_type

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cd07018: S49_SppA_67K_type 
Click on image for an interactive view with Cn3D
Signal peptide peptidase A (SppA) 67K type, a serine protease, has catalytic Ser-Lys dyad
Signal peptide peptidase A (SppA; Peptidase S49; Protease IV) 67K type: SppA is found in all three domains of life and is involved in the cleavage of signal peptides after their removal from the precursor proteins by signal peptidases. Members in this subfamily contain an amino-terminal domain in addition to the carboxyl-terminal protease domain that is conserved in all the S49 family members (sometimes referred to as 67K type), similar to E. coli and Arabidopsis thaliana SppA peptidases. Unlike the eukaryotic functional homologs that are proposed to be aspartic proteases, site-directed mutagenesis and sequence analysis have shown that members in this subfamily, mostly bacterial, are serine proteases. The predicted active site serine for members in this family occurs in a transmembrane domain. Mutagenesis studies also suggest that the catalytic center comprises a Ser-Lys dyad (both residues absolutely conserved within bacteria, chloroplast and mitochondrial signal peptidase family members) and not the usual Ser-His-Asp catalytic triad found in the majority of serine proteases. Interestingly, the single membrane spanning E. coli SppA carries out catalysis using a Ser-Lys dyad with the serine located in the conserved carboxy-terminal protease domain and the lysine in the non-conserved amino-terminal domain.
Statistics
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PSSM-Id: 132929
View PSSM: cd07018
Aligned: 137 rows
Threshold Bit Score: 149.613
Threshold Setting Gi: 197736998
Created: 25-Nov-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:active site residues [active site]
Evidence:
  • Comment:The N- and C-terminal halves of SppA in E. coli are tandem repeats and the Ser/Lys dyad is arranged such that the nucleophile Ser is located in the C-terminal half whereas the general base Lys is located in the N-terminal half.
  • Structure:3BEZ: Escherichia coli Signal peptide peptidase A (SppA)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
3BEZ_A        34 LLLDISGvivdkpdssqrfsklsrqllgassdrlqensLFDIVNTIRQAKDdrnitGIVXDLKNfag--------gdqPS 105  Escherichia coli
EDY84571      49 LVFDMSAsiqdrpvgmtrdaf---ideaiggqrlrkisLRTLTRGIRAAASddrieGLLLTGSFavegy-----gsgfAA 120  Verrucomicrobi...
NP_604168     44 VLINLADsynerllks-------------nlfeddsinFYTLLQSIEAISYddrveGIILKLNGdsl---------syAQ 101  Fusobacterium ...
YP_001618986 346 VRVRLDStpg-------------------------vrrHTRLLQQLWRLAEdpevsGVALVLRAepa--------sslAH 392  Sorangium cell...
YP_002165776  36 DKVSLKGiktvvfnlnelved----ymistvsinktlsHEAVLKALENLVNdkkieKIIIDVDEvdl---------srVH 102  Fusobacterium ...
ZP_01908517  340 TRISLAGksd-------------------------drdLAEVVWTFDGLARrg-pgVILLEVDGsdf---------glAQ 384  Plesiocystis p...
XP_759776     95 VWKVYDGsivesrgppsvsali--napaageeaprvmeMFEVIRALKWAQAddrikGILADFSGlhlpssvtpnrlglAQ 172  Ustilago maydi...
XP_001732381  48 TWRVFDGsivevrqaqsigqll--aqptpgdqpvrileLHQVIETLRRAKDdarirGIVADFSQmhvpravlrqplglAQ 125  Malassezia glo...
YP_001233201  74 LLRRATGat---------------------------ptLEQTIAAIDRAATdprvrGIDILLGGgcc---------glTT 117  Acidiphilium c...
YP_761326     46 LTLDLNAaypdqapagg------------laalsgtpgFIDLLLKLKEAESddsvkGLFIRGADygf---------gtTR 104  Hyphomonas nep...
Feature 1                                             #                                          
3BEZ_A       106 XQYIGKALKEfrd-sgKPVYAVGe----------NYSQGQYYLASFankIWLSP--QGVVDl--hGFATNGLYYKSLLDK 170  Escherichia coli
EDY84571     121 LKEVHEAVEVfse-sgKPVWAYVv----------YPTARDMYVISAadtIYMNP--EGVVSd--tGMSMSYPYLGGFMKK 185  Verrucomicrobi...
NP_604168    102 SEELAHEISMara-anKKIIAYFe----------NVGRKNYYLASYaneIYMPSanSTNVNi--yPYFKENFYIKGLADK 168  Fusobacterium ...
YP_001618986 393 AEEVSDAIRNlrs-hgKKVLCHLe----------DAGGRSLHVCSSadaIAMNP--AGGLRf--aGMSSSYFYFGGLLNK 457  Sorangium cell...
YP_002165776 103 IEEIKEIFEKls--vnKEIIAIGt----------TFDEYSYQVALLankIYMLN--TKQSSlyfrGYEYKEPYFKNILTN 168  Fusobacterium ...
ZP_01908517  385 LEEVREAVLRyra-qgGKVVAYLe----------GASTFEYFLAAScdrIYAHP--QQGLEl--rGVLRNTFYFGELLGR 449  Plesiocystis p...
XP_759776    173 IEELMQAIHEfki-akKQQHGDKarpsi-awadtFNSQGSFLLASAfdeLWMQP--AGSIPl--tGLSAQIPFFKKVLDY 246  Ustilago maydi...
XP_001732381 126 TEELLTALHEfr--taKQDQFGKdqpatvawsdtFDSQTAFLLATGfdrVYMQS--SGQVPl--vGLGSSLTFFRRMLQW 199  Malassezia glo...
YP_001233201 118 AEELHDALARfraishRQVVARAmsf------dgAEGLGAYIVATAanrIELSD--AGDFGv--tGLALQSPFAADLLKM 187  Acidiphilium c...
YP_761326    105 AEELREAIQSfka-sgKFVIAHSqgm------fgSSGPSAFHSISAadeIWMQP--GTDLMv--tGVVFETEFYKGLFEN 173  Hyphomonas nep...
Feature 1                                                                                        
3BEZ_A       171 lKVSTHVFRVgtYKSAVEPFird-dxsPAAREADSRWIGELWQNYLNTVAAnrqipaeqvfpgaqGLLEGLTk---TGGD 246  Escherichia coli
EDY84571     186 yGVGVQVTRAgdYKAAAESFvle-gmsEPSREANAAVLEDFWSEYLSVIANgag--------ieaAEYERMLn--eLGML 254  Verrucomicrobi...
NP_604168    169 fGVKFNIIHVgdYKSYMENLasn-tmsKEAKEDTVRVLDKNYNNFLDVVSLnrk--------inrEDLDKIIkdgeLVAA 239  Fusobacterium ...
YP_001618986 458 lGVQADIVRIgaHKLAAEQFare-tstRVGKDDHQALLDQLSDAFLAEVSAgrr--------ipvPELERRIa---RGPF 525  Sorangium cell...
YP_002165776 169 lGVTVNTLHIgdYKVAGESFsnd--kmSEEKKESLINIKETLFQNFINLVKekr---------kvDITNEILsg-dLIFA 236  Fusobacterium ...
ZP_01908517  450 lGVRAEFVRVaeYKGTPERAsrg-epsAPVARANRAYVTDTWNHVVRLIGHsrar-------dpaVVSEWID----RAVW 517  Plesiocystis p...
XP_759776    247 fGIRVLAEARreYKSMISTFsredsltAPQIHNEAELLGELNRGLVHAIAVnrfpde--dpeltsSKVEAWMk---QGPF 321  Ustilago maydi...
XP_001732381 200 lGVRVLSETRneYKSVTSAFvhd-elpPEQLANLSDVLGGLQHNMARLLGQnrfseqg-pphvatAKAEHVLr---HGPY 274  Malassezia glo...
YP_001233201 188 aGVEAQFEHIgkYKTYPELFtrs-gpsAAATEMMNSLAGSLYDSALVPIAArlk--------rspDQVKALFd---QAPF 255  Acidiphilium c...
YP_761326    174 iDLQPQVYPFyeYKNAPNSYnet-sytEPHRMAMEALATSIWTTALEEIAEdrg--------tsaGQLRAALe---SGPK 241  Hyphomonas nep...
Feature 1                                  
3BEZ_A       247 TAKYALENKLVDALasSAEIEKALTK 272  Escherichia coli
EDY84571     255 TAVDAERLGLVDELmfTDELIAALRK 280  Verrucomicrobiae bacterium DG1235
NP_604168    240 SSADLMNNNLIDKYvyWDNVISMVGG 265  Fusobacterium nucleatum subsp. nucleatum ATCC 25586
YP_001618986 526 LAQEARQSGLVDMLayDDELGRVIDE 551  Sorangium cellulosum 'So ce 56'
YP_002165776 237 NSEKAIQLGLIDGLstYEEIGIDYNE 262  Fusobacterium nucleatum subsp. polymorphum ATCC 10953
ZP_01908517  518 TPEDARRMGLIEGLawPEELDARLEG 543  Plesiocystis pacifica SIR-1
XP_759776    322 STREATQLGLINGAafKRDIIKRLID 347  Ustilago maydis 521
XP_001732381 275 SASEALAAGLIDGIchRHDITPSLDV 300  Malassezia globosa CBS 7966
YP_001233201 256 SAAQAKQDGLVDTVlpLSAEVAHVRG 281  Acidiphilium cryptum JF-5
YP_761326    242 PAETALELKLVDKLgwPEDAEEAAIA 267  Hyphomonas neptunium ATCC 15444

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