2C45,1PQH


Conserved Protein Domain Family
Asp_decarbox

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cd06919: Asp_decarbox 
Click on image for an interactive view with Cn3D
Aspartate alpha-decarboxylase or L-aspartate 1-decarboxylase, a pyruvoyl group-dependent decarboxylase in beta-alanine production
Decarboxylation of aspartate is the major route of beta-alanine production in bacteria, and is catalyzed by the enzyme L-aspartate decarboxylase (ADC), EC:4.1.1.11 which requires a pyruvoyl group for its activity. The pyruvoyl cofactor is covalently bound to the enzyme. The protein is synthesized as a proenzyme and cleaved via self-processing at Gly23-Ser24 to yield an alpha chain (C-terminal fragment) and beta chain (N-terminal fragment), and the pyruvoyl group. Beta-alanine is required for the biosynthesis of pantothenate, in which the enzyme plays a critical regulatory role. The active site of the tetrameric enzyme is located at the interface of two subunits, with a Lysine and a Histidine from the beta chain of one subunit forming the active site with residues from the alpha chain of the adjacent subunit. This alignment model spans the precursor (or both beta and alpha chains) of aspartate decarboxylase.
Statistics
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PSSM-Id: 132994
View PSSM: cd06919
Aligned: 58 rows
Threshold Bit Score: 149.194
Threshold Setting Gi: 119872877
Created: 8-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetetramerization
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Gly23-Ser24 form the cofactor pyruvoyl by intramolecular non-hydrolytic serinolysis
  • Comment:Structures demonstrating the active configuration with the covalently bound cofactor are not included in this alignment model, as they are proteolytically cleaved and split into two smaller chains, represented by the stretches in this alignment which are N- and C-terminal to Gly23-Ser24
  • Structure:1PQH: A Ser24Thr mutant of aspartate 1-decarboxylase from Escherichia coli binds malonic acid, indicating the location of the substrate in the native active enzyme
    View structure with Cn3D
  • Citation:PMID 9546220

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               # #            ##                                #                       
2C45_A         2 LRTMLKSKIHRATVTCADLHYVGSVTIDADLMDAADLLegEQVTIVDIdnGARLVTYAITGErgSGVIGINGAAAHLVHp 81  Mycobacterium t...
1PQH_A        19 IRTMLQGKLHRVKVTHADLHYEGTCAIDQDFLDAAGILenEAIDIWNVtnGKRFSTYAIAAErgSRIISVNGAAAHXASv 98  Escherichia coli
YP_658098      2 RRWLLKSKLHRARVTGTEKDYEGSISIDAALLSEADIAvgEQVQVVNVtnGERFETYTIEGE--SRQMELNGAAARLAEt 79  Haloquadratum w...
ABK80583       2 QRIMLKSKLHRARVTATEIDYEGSIAIDEDLLDRVGIMplEQVHIFNInsGARFITYVINAErgSGIFSINGAAARLTQi 81  uncultured mari...
CAO80944       2 LRQILLAKLHRATVTECDLNYNGSIKIDEELLEKSGMRefERVDVFDItnGNRFSTYIIVGErgSGVIGINGAAARLVQv 81  Candidatus Cloa...
ZP_02012185   14 HRTFLKTKLHRATLTAADPDYEGSISIDRALCRAAGMLeyEQVDVLDInnGARFTTYVIYGE--PGQVQVNGAAARLVRp 91  Opitutaceae bac...
YP_001735762   3 QIKLMHAKLHHLRVTQAELDYVGSITIDQALIEKVGILplEEVNIWNVenGNRLTTYVLPGErdSGVVCLNGAAAHLCSp 82  Synechococcus s...
NP_106421      1 MRKLVAGKLHGIHVTEANLNYHGSITLDPDHCEAAGILpmEFVEIWNKnsGARISTYVILGErgSRCCILNGAAARTCQp 80  Mesorhizobium l...
Q55382         3 SIRLMHAKLHRVCVTEANVNYVGSITIDPVMLERVGLLplEEVDIINLsnGNRFSTYVLPGQahSKEICPNGGAALLCQp 82  Synechocystis s...
NP_066682     26 VEKYVGAKLHGLRVTDAKLNYHGSITIDADFCREVGLKplEYVEIWNKmsGARISTYVLYGDpgSRCCILNGAAARTCQq 105 Agrobacterium r...
Feature 1                                         
2C45_A        82 GDLVILIAYATmdda--rartYQPRIVFVDAYN 112 Mycobacterium tuberculosis
1PQH_A        99 GDIVIIASFVTmpde--eartWRPNVAYFEGDN 129 Escherichia coli
YP_658098     80 GDVIIVISYGLyvk----deqPEPTVLLLDEEN 108 Haloquadratum walsbyi DSM 16790
ABK80583      82 NDRIIVAAYGMintd---qekHNAQVTLLDESN 111 uncultured marine Nitrospinaceae bacterium
CAO80944      82 GDKVIVMNYGImdet--eienHKPCIILLNDNN 112 Candidatus Cloacamonas acidaminovorans
ZP_02012185   92 GDLVIVLAYCRldek--evpaHKPRVVLMGPGN 122 Opitutaceae bacterium TAV2
YP_001735762  83 GDRLIVAAYELrdrrdvlaqgHTAKVILADEQN 115 Synechococcus sp. PCC 7002
NP_106421     81 DDPIIVCNSIYldea--hitsLKPRIVTFDQDN 111 Mesorhizobium loti MAFF303099
Q55382        83 GDRLIIFAYELcdrrevlqkgHQAKVLVTNESN 115 Synechocystis sp. PCC 6803
NP_066682    106 GDEIIIASSVFcevd--diikLKPRVLVFGEKN 136 Agrobacterium rhizogenes

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