Conserved Protein Domain Family
GT_MraY_like

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cd06912: GT_MraY_like 
This subfamily is composed of uncharacterized bacterial glycosyltransferases in the MraY-like family. This family contains both eukaryotic and prokaryotic UDP-D-N-acetylhexosamine:polyprenol phosphate D-N-acetylhexosamine-1-phosphate transferases, which catalyze the transfer of a D-N-acetylhexosamine 1-phosphate to a membrane-bound polyprenol phosphate. This is the initiation step of protein N-glycosylation in eukaryotes and peptidoglycan biosynthesis in bacteria. The three bacterial members MraY, WecA, and WbpL/WbcO, utilize undecaprenol phosphate as the acceptor substrate, but use different UDP-sugar donor substrates. MraY-type transferases are highly specific for UDP-N-acetylmuramate-pentapeptide, whereas WecA proteins are selective for UDP-N-acetylglucosamine (UDP-GlcNAc). The WbcO/WbpL substrate specificity has not yet been determined, but the structure of their biosynthetic end products implies that UDP-N-acetyl-D-fucosamine (UDP-FucNAc) and/or UDPN-acetyl-D-quinosamine (UDP-QuiNAc) are used. The prokaryotic enzyme-catalyzed reactions lead to the formation of polyprenol-linked oligosaccharides involved in bacterial cell wall and peptidoglycan assembly.
Statistics
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PSSM-Id: 133467
View PSSM: cd06912
Aligned: 38 rows
Threshold Bit Score: 93.8458
Threshold Setting Gi: 154249139
Created: 17-Nov-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Mg++ bindingputative
Feature 1:Mg++ binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                ##                      
YP_001130131  43 DGVQKFHTaPTPRIGGLAIFAAlfvawlllpkgs--gmllglmliasLPAFVAGFIEDLTKrvgvrERLLATILSGVAay 120 Prosthecochlori...
YP_001409771  36 DYKLKTHEkATPYLGGVAFVLSfliftpys----------lfrklyiTVLSLMGLYDDIKStn-pwIRLIVEFLIGFLvs 104 Fervidobacteriu...
YP_001568679  34 DNKLKVHKsPIPYLGGVAVFLSilpfyynd----------igfiipaSILTFIGLYDDIKSvs-pyIRLFAEFIVVSMai 102 Petrotoga mobil...
NP_228614     31 DGVLKPHGrETPYLGGLGIFVGvlpflwnd----------tvvllsaSIALALGLMDDLFSls-pfLRLIAEFGISLLlv 99  Thermotoga mari...
NP_229349     27 PDSRKSHGrAVPPVGGVSIFLTlliferd-----------npfflfsIPLFLLGLLDDLFDls-yrIKLAVTALVAVWfs 94  Thermotoga mari...
YP_001470635  26 PNSRKIHTiPIPLTGGVAIFIAllvtekn-----------lltiipvVLAFSLGFLDDIEDls-ykIKLPVQFLIALSal 93  Thermotoga lett...
YP_001305915  27 PNKRKNHNiPTPQIGGVILFTIlsiyfn-------------fpflilLSLLIFGILDDMFKls-ykQKFTFEVLIAIYlv 92  Thermosipho mel...
YP_001409964  34 PSERKKHNkPVPLIGGVILFLMvlflsklgiy-----enniisdvvfYIVFAIGIIDDLLEip-yyLKLLLQFIAGIFyi 107 Fervidobacteriu...
YP_265991     33 PNSEKTHKnPTPAIGGVIFLLLyisiffqylifdelnyfhsklvvasLLIFFIGYYDDLKDnn-pyLKTFFFISVIFIfl 111 Candidatus Pela...
ZP_03330664   31 AGKLKPHEtAIPYTGGTAILLSlipwlihe----------pgymvvvVSLWAIGFIDDVKGln-psLRLMIELLAGFLfs 99  Thermotogales b...
Feature 1                                                                                        
YP_001130131 121 yltgysltrvdvpg--fdvllqwmpftvaftAFAVGGVANSVNIIDGFNGLAGGVLMICFGmlaaiawqvgdthlaklci 198 Prosthecochlori...
YP_001409771 105 trflts------------------pveifvaTFFYAFLINSVNMMDGMDGICAITSAIAAFgliwtvmfpydk---fyli 163 Fervidobacteriu...
YP_001568679 103 yfvggii----------------epfqffilVLTGIALVNGVNMVDGMDGVCAGTAIVSLLffslisknye-------ll 159 Petrotoga mobil...
NP_228614    100 wrfigld----------------nllvstfwLFFVVVLINSVNMMDGMDGLCGSLVALSALsyfflvrgvffe---nlsl 160 Thermotoga mari...
NP_229349     95 tavtievsi------------fgarihpvffVIWFVGMVNAFNVVDGLDGLLSGISLFSSLmigers------------- 149 Thermotoga mari...
YP_001470635  94 fvvsqkitffg--------itlnntlgrvieLFWFMSMLNAINMIDGIDGLACSTTMISSLlskeys------------- 152 Thermotoga lett...
YP_001305915  93 wkypftlfg------------ykniftdifaIFWVVAFFNGFNMIDGFNGLSTGISIIYLStlknyn------------- 147 Thermosipho mel...
YP_001409964 108 nshhlyflg------------fdnlttkivtFLFFIVIVNAFNLIDGVNGLLIGIAIIYSLmtlnfe------------- 162 Fervidobacteriu...
YP_265991    112 ffnqeilikkilisfidvtyvlnnffsyiftILCFWLLMNSFNLTDGFNGVALSLAIIFFTslitlfdlsevd--hffii 189 Candidatus Pela...
ZP_03330664  100 lyylnn-----------------sffeslflAFLFAAVVNAFNMIDGMDGVCSGVVVVSALvlvivfqlhp------lll 156 Thermotogales b...
Feature 1                                             
YP_001130131 199 lfilcvaGFMFLNFPFGkiFMGDGGAYLMGFLLAWTA 235 Prosthecochloris vibrioformis DSM 265
YP_001409771 164 slmgallAYLIYNFPPAkiFMGDMGSYTIGAILGVSV 200 Fervidobacterium nodosum Rt17-B1
YP_001568679 160 ifafailGFLLYNLPPAkiFLGDAGSYLLGFTLFYSF 196 Petrotoga mobilis SJ95
NP_228614    161 sllgaslGYLVFNFPPAriFMGDAGSYLMAVLLSAIV 197 Thermotoga maritima MSB8
NP_229349    150 -lafsiiGFLPWNLPDAkvFLGNSGSFLLGAYLSTAS 185 Thermotoga maritima MSB8
YP_001470635 153 -lalavaGFLPFNLPRAksFLGNSGATLLGIYLPMRV 188 Thermotoga lettingae TMO
YP_001305915 148 -lalaylPIFIFNFFGKl-FIGESGVLITSYLLLLSA 182 Thermosipho melanesiensis BI429
YP_001409964 163 -iallllILLFFNFQESl-FMGDSGAFLVAYILLNSA 197 Fervidobacterium nodosum Rt17-B1
YP_265991    190 niifflvIILIFNLFGKl-FLGNNGAYLISFILAVYL 225 Candidatus Pelagibacter ubique HTCC1062
ZP_03330664  157 afagtffGFFVWNFPPAkiFLGDQGSYIAGAFMGMML 193 Thermotogales bacterium TBF 19.5.1

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