1KMD


Conserved Protein Domain Family
PX_SNARE

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cd06897: PX_SNARE 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of SNARE proteins from fungi
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. This subfamily is composed of fungal proteins similar to Saccharomyces cerevisiae Vam7p. They contain an N-terminal PX domain and a C-terminal SNARE domain. The SNARE (Soluble NSF attachment protein receptor) family of proteins are integral membrane proteins that serve as key factors for vesicular trafficking. Vam7p is anchored at the vacuolar membrane through the specific interaction of its PX domain with phosphatidylinositol-3-phosphate (PI3P) present in bilayers. It plays an essential role in vacuole fusion. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction.
Statistics
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PSSM-Id: 132807
View PSSM: cd06897
Aligned: 22 rows
Threshold Bit Score: 105.817
Threshold Setting Gi: 164660166
Created: 12-Jul-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                      ###                         ##    
1KMD_A         6 LRIKVDDVKIn----PKYVLYGVSTP--------------NKRLYKRYSEFWKLKTRLERDVGst---iPYDFPEKPGVl 64  baker's yeast
XP_501902     18 MDISIPTYTV-----SSPTSFTVQVDlegggdvrhrgeenKYTLTKRYSDFVALVSQLEDEVGrt---vEVALPPKSWFk 89  Yarrowia lipoly...
XP_001647022   8 VEVNIEDVKIv---nSKYASYGILIIlksvdg---siqyyDKKVYRRYSEFFKLKKNLEREFGve---iPYEFPDRLFVi 78  Vanderwaltozyma...
XP_001523859   1 MSITIPAEEEi----NGSTYYQINVKlp----------lrSYTIKKRYSDFDNLVKTLCHHIGinrkdfPYTLPAKRIHw 66  Lodderomyces el...
XP_002172574   1 MKVTIPTTSSssdlrAPWTWYHILIEypn---------grKMTVKRRYREFCELHETLEKQSSqfd-taVPPIPPKSWFs 70  Schizosaccharom...
XP_001384660   3 EIISIPTTSTh----GGATYYHVAIKlp----------lrSLTVQRRYSEFEDLVNGLCDNLGinvkdfPYELPSKRINw 68  Pichia stipitis...
XP_451073      7 CEVSINDVTIv---eKRYALYHIIVNvirsk-----edysEYKSERRFKDFLQLKKQLEYECSge---lPYELPGRRLLw 75  Kluyveromyces l...
NP_587792      3 LKIKIPETSQssdeySRWTVYHIEVAfpn---------ggKHVVFRRFNEFVALDAQIRPNDYns---rLCKLPSKSWVs 70  fission yeast
NP_985293      7 VHVTISDASVv---nKSYVQYTTRVRvqhgs-----esavEYKCRRRFSEFLQLKLDLEREFDae---iPYDFPARKFNl 75  Ashbya gossypii...
AAX78217       2 VEVRIPSTIVl----NDYHLYNIEIQfes--------gvhVHKLSKRFSDFVEFKKELEDTLHkt---iPYSLPSKFTAl 66  Pichia angusta
Feature 1                        #                                 
1KMD_A        65 drrwqrryDDPEMIDERRIGLERFLNELYNDrfdsRWRDTKIAQDFLQLS 114 baker's yeast
XP_501902     90 -------sKNVEFLDDRRRGLEVFLRRLVKIp---HFYSSAALLEFLQLQ 129 Yarrowia lipolytica CLIB122
XP_001647022  79 wv---gsnIDPDVIESRRKKLSKFLYDLLNDsfdsKWKNSDYVSEFLDLR 125 Vanderwaltozyma polyspora DSM 70294
XP_001523859  67 -------lNKSNVVEERKQEFTHFLNNMLLDl---SLRNEPEFLLFLQLP 106 Lodderomyces elongisporus NRRL YB-4239
XP_002172574  71 s-----tvTNSSLRESRRLQLEEFLGRLVYS----DWFHLQVVKDFLELR 111 Schizosaccharomyces japonicus yFS275
XP_001384660  69 f-----ksNTQNIISERKAELSRFLNQAIRDs---TIQNSALLHKFLSLP 110 Pichia stipitis CBS 6054
XP_451073     76 srt--ansCDPDVVEERRVKLRQFLSDLLNDsfetKWRNSSLVAQFLNLP 123 Kluyveromyces lactis NRRL Y-1140
NP_587792     71 s-----tvTNEKLRESRRLALQAYVQCLSET----PWIKMPVVKKFLNIK 111 fission yeast
NP_985293     76 wnm-ksrsCDPAVVDERRERLTSFLTDLLNDsfdvRWKTSPTLCAFLNMP 124 Ashbya gossypii ATCC 10895
AAX78217      67 y------qSRATTIEQRRVGLAEFLNGLLNDe---QLRTHKLVGEFLYLP 107 Pichia angusta

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