Conserved Protein Domain Family
PX_SNX5_like

?
cd06892: PX_SNX5_like 
The phosphoinositide binding Phox Homology domain of Sorting Nexins 5 and 6
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Members of this subfamily include SNX5, SNX6, and similar proteins. They contain a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to other sorting nexins including SNX1-2. The PX-BAR structural unit helps determine the specific membrane-targeting of some SNXs. SNX5 and SNX6 may be components of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, acting as a mammalian equivalent of yeast Vsp17p.
Statistics
?
PSSM-Id: 132802
View PSSM: cd06892
Aligned: 12 rows
Threshold Bit Score: 196.885
Threshold Setting Gi: 119630668
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
putative
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                 ###                                    
Q9Y5X3        29 PSLQIDIPDALSERDKVKFTVHTKTTLPtfq--spEFSVTRQHEDFVWLHDTLIETTDYAGLI--------------IPP 92  human
XP_791140     35 TSLQVDISDALSERDKVKFTVHTKTTLPefs--esEFSVQREHEEFIWLHTMYEENEDYGGII--------------IPP 98  purple urchin
XP_001638873  18 SSLNVDISDALSERDRVKFTVHTKTTLPdfk--esDFSVVREHEEFVWLHDRCVENEEYAGII--------------IPP 81  starlet sea ane...
NP_507518    102 EAICVDISDALSEREKVKYTVHTRTRLQem---kpETAVVREHEEFLWLHGTLEDNENYAGFI--------------IPP 164 nematode
Q9VLQ9        72 NALHVEISDALSEKEKVKFTVHTRTTLPgfs--kkDNNVVRQHEEFVWLHDRIEENDDYAGYI--------------IPP 135 fruit fly
XP_002113191  28 ACLVIEISDALSERDKVKFTIHTRTTLPefk--lhDFSVTRDHEEFIWLYDRYQDNENLEAYI--------------IPH 91  Trichoplax adha...
XP_001750051  26 DALQISIADALNEQDIVKFTVHTKTSLErfq--krDFQVTRQHEEFVWLHDRLVEDPDYAGLL--------------IPP 89  Monosiga brevic...
XP_002130614  31 NSLLVDISDALSEKDKVKFTVHTKTTMKefk--gnEFSVVRQHEEFVWLHDSYVDDERYAGLLdkgilvrmlkllkkIPP 108 Ciona intestinalis
XP_001480832  40 FTKFNADEFEDMVAEKQLIPDGCGTTLStfq--spEFSVTRQHEDFVWLHDTLTETTDYAGLI--------------IPP 103 house mouse
EAX10263      29 PSLQIDIPDALSERDKVKFTVHTKTTLPtfq--spEFSVTRQHEDFVWLHDTLIETTDYAGLI--------------LSV 92  human
BAE89581      14 SCASVDLQGDSSLQVEISDAVSERDKVKftvqtktEFSVVRQHEEFIWLHDAYVENEEYAGLI--------------VRR 79  crab-eating mac...
AAD27829      30 AALQVDISDALSERDKVKFTVHTKSSLPnfk--qnEFSVVRQHEEFIWLHDSFVENEDYAGYI--------------IPP 93  human
Feature 1          ##                                                #                           
Q9Y5X3        93 APTKPDFDGPREKMQKLGEGEGs------MTKEEFAKMKQELEAEYLAVFKKTVSSHEVFLQRLSSHP-------VLSKD 159 human
XP_791140     99 APPRPDFDASREKLQKLGEGEGt------MTKEEFNKMKQELEAEYLATFKKTVAMHEVFLQRLAAHA-------ILRND 165 purple urchin
XP_001638873  82 APPKPDFDVSREKLQKLGEGEGn------MTKEEFQKMKQELEAEYLATFKKTVAMHEVFLTRLASHP-------SLRKD 148 starlet sea ane...
NP_507518    165 APPKPNFDSSREKLQKLGEGEAt------MTKEEFLKMKHDLEQDYLAQFKKTVAMHEVFLQRIAAHP-------VFKND 231 nematode
Q9VLQ9       136 CPPRPDFDASREKLQRLGEGEGn------MTKEEFKKMKSELEAEYLATFKKTVAMHEVFLRRLASHP-------VFRVD 202 fruit fly
XP_002113191  92 PPPKPDFDTAREKLQRLGESEGv------LTKEEFDKMKQELEAEYLATFKKTVAMHELFLTRLASHS-------VLRND 158 Trichoplax adha...
XP_001750051  90 CPPKPDFSQSHGKLAKLQAGDSs------MTPEEIEKLKQEIQGEYLAAFQKTVAMHEVFLLRLVTHP-------IFREN 156 Monosiga brevic...
XP_002130614 109 SPLRPDFEASRDKLAKLSEGEGs------MTKEEFSRMKQELEAEYLAIFKKTVAMHEIFLCRLASHP-------TLKNS 175 Ciona intestinalis
XP_001480832 104 APTKPDFDGPREKMQKLGEGEGs------MTKEEFAKMKQELEAKDRNFHVFLEYDQDLSVRRKNTKEmfggffkSVVKS 177 house mouse
EAX10263      93 RRKNTKEMFGGFFKSVVKSADEvlftgvkEVDDFFEQEKNFLINYYNRIKDSCVKADKMTRSHKNVADd------YIHTA 166 human
BAE89581      80 GRAGAGRDRQSTWVRTKGYGGC-------LGSERSPSSVPPLSRSPQPLRGQTLRLQGKSYRNWARGRees--raCLVCL 150 crab-eating mac...
AAD27829      94 APPRPDFDASREKLQKLGEGEGs------MTKEEFTKMKQELEAEYLAIFKKTVAMHEVFLCRVAAHP-------ILRRD 160 human
Feature 1                  
Q9Y5X3       160 RNFHVFLEYD 169 human
XP_791140    166 HNFRVFLEYK 175 purple urchin
XP_001638873 149 SNFKVFLEYK 158 starlet sea anemone
NP_507518    232 QNFRIFLQYE 241 nematode
Q9VLQ9       203 QHLKVFLEYD 212 fruit fly
XP_002113191 159 SNFRVFLEYN 168 Trichoplax adhaerens
XP_001750051 157 ARLQAFLEFQ 166 Monosiga brevicollis MX1
XP_002130614 176 HNFRVFLEYE 185 Ciona intestinalis
XP_001480832 178 ADEVLFSGVK 187 house mouse
EAX10263     167 ACLHSLALEE 176 human
BAE89581     151 WPQAPGLDVQ 160 crab-eating macaque
AAD27829     161 LNFHVFLEYN 170 human

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap