Conserved Protein Domain Family
PX_Vps17p

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cd06891: PX_Vps17p 
The phosphoinositide binding Phox Homology domain of yeast sorting nexin Vps17p
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Vsp17p forms a dimer with Vps5p, the yeast counterpart of human SNX1, and is part of the retromer complex that mediates the transport of the carboxypeptidase Y receptor Vps10p from endosomes to Golgi. Similar to Vps5p and SNX1, Vps17p harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. The PX-BAR structural unit helps determine specific membrane localization.
Statistics
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PSSM-Id: 132801
View PSSM: cd06891
Aligned: 14 rows
Threshold Bit Score: 165.988
Threshold Setting Gi: 71005760
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
P32913        85 QQPSQPSErvilp---------------ersdEKKKYTLLAKVTGLERfgsatgkkenPTIIFDCSTN-LPTFRKQQYKN 148 baker's yeast
XP_461754     60 EKLSEEEMrkll-----------------perFTDKYQINIKLTAIEKnka-----gnPILRFDATVKgLPKFRSETYKD 117 Debaryomyces ha...
EDP51463     100 QYTNEPRSqlpgrsqt---------garqnakNGPAYKIQAKITGLERtgk-----kdPILRFDVHTN-VPKFRTTQYRD 164 Aspergillus fum...
XP_447566     59 TQPKQMESeetkkknaekqqeilkkliperfeQKDKYNVVIKVIGLERvgslsnkkenPTIIFEVNTN-LPTFRKKNHKN 137 Candida glabrat...
XP_001876617  62 QIYGQPEAglisprel--------vgsngvgyEKPGPYLRVRITGLDRnr------rdILVKLDAQTN-LSNFTGTTYRN 126 Laccaria bicolo...
XP_566931     95 FQQSQQETpagev---------------qqerKAPRVYLRVRIGALERnk------kdLLIRFDASTN-LSTYKNGMYRN 152 Cryptococcus ne...
Q9URW7        73 EATMNPSAassttg------------ensisqTGSGPFLRIRIVDIEAens-----kdPVIKMNVQTT-LPAYRSKLYKN 134 fission yeast
XP_718434     51 EPSPDPNSesiltede--------lrkliperFTTKYSLSIQLVEIEKnkp-----gnPILKMNVKVKgLPRYRQSIYKD 117 Candida albican...
XP_002172238 160 SQENEERStvsk----------------knatSEPKFMVSFRIASIEIvdk-----hdPILKMNVTTN-CPGYRSKHYRD 217 Schizosaccharom...
XP_001643018  50 ETDKAAEApkknv--------------lpersSNQKFNITVKVTGLERagstnnkkenPTIVLDLNTN-LPTFRKQTHKK 114 Vanderwaltozyma...
Feature 1           ###                      ##              #                              
P32913       149 VKKSYEEFHQLFKYLNVAIQESFVPTLPSAYTtfGINSEEDRMKVTRNFQLWFNRLSQDpLIIRNEEVAFFIESD 223 baker's yeast
XP_461754    118 IRRTYNEIVKFNKYLTVSNLEVFVPVIPLAITsyPANGEDEAKQLHSIWQEWFNRITSNpILIRDEEFVYFIEND 192 Debaryomyces hanseni...
EDP51463     165 VRRTHAEFAKLADHLISANPEVLVPAVPPPLTpaGAGTEEDEIRVKASMQRWLNIVMSNeILMQDDEVVLFVESD 239 Aspergillus fumigatu...
XP_447566    138 VRKSLDEFRNLFKYLNATLSESFIPSLPAPFTnyGINTKEDYKKTVNNFQVWFFRLCANpLIIRNEELAFFIESD 212 Candida glabrata CBS...
XP_001876617 127 VSRSYLEFQQFYETLVQSNPQTIIPALPLAQTs-APTDEEDDRLVKIMLQRWITRVSEDsILIQDEDLRSFIESD 200 Laccaria bicolor S23...
XP_566931    153 MQRSYVEFQKFAEQLSLVCPQTIIPALPLPSTs-ASTDEEDDRLVRIALQRWFSRVCEDpVLIRQDEVRSFIESN 226 Cryptococcus neoform...
Q9URW7       135 VRRTHAEFKKFAKYLISTHPECLIPAVPEAKTsvSSGIKEDLIYLKSGLQSWLNYVSTNpNLLYDPELQLFVESD 209 fission yeast
XP_718434    118 VRRTFNEVVKFNRYLIVSNLEVFVPVIPSAITsyPTGGEDERKKLFVEWQEWLDRITSNpILIRDEEFVYFVEND 192 Candida albicans SC5314
XP_002172238 218 VRRTYGELCKLARYLTFMHPECLIPAVPPSVGsaGSGSIEDNRRLKESLQSWLDYVGRNpYLVTDTETKLFVESD 292 Schizosaccharomyces ...
XP_001643018 115 VKKTMEEFQQLFKYLNGAIQESFIPALPIPFTnyGINNREDLDKTVHNFQLWFDRILSDpLILRNEELAFFIESD 189 Vanderwaltozyma poly...

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