2V6V,2CZO


Conserved Protein Domain Family
PX_Bem1p

?
cd06890: PX_Bem1p 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of Bem1p
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Members of this subfamily bear similarity to Saccharomyces cerevisiae Bem1p, containing two Src Homology 3 (SH3) domains at the N-terminus, a central PX domain, and a C-terminal PB1 domain. Bem1p is a scaffolding protein that is critical for proper Cdc42p activation during bud formation in yeast. During budding and mating, Bem1p migrates to the plasma membrane where it can serve as an adaptor for Cdc42p and some other proteins. Bem1p also functions as an effector of the G1 cyclin Cln3p and the cyclin-dependent kinase Cdc28p in promoting vacuolar fusion. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of Bem1p specifically binds phosphatidylinositol-4-phosphate (PI4P).
Statistics
?
PSSM-Id: 132800
View PSSM: cd06890
Aligned: 22 rows
Threshold Bit Score: 94.2777
Threshold Setting Gi: 68474314
Created: 11-Jul-2008
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:The PX domain of Bem1p binds phosphatidylinositol-4-phosphate (PI4P).
  • Comment:based on the structures of phosphoinositide-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                              ###                                       
2V6V_A        19 VKASVESFGLe-dEKYWFLVCCELsn----gKTRQLKRYYQDFYDLQVQLLDAFPAEAGklrda-----------ggqws 82  baker's yeast
NP_986354    134 CWCCVTCCGIr-eSRVWYRIECMLrs----gYQRTLCRYYHDFYWLHVGLIGRLQQLGLgv------------------d 190 Ashbya gossypii...
XP_454397    192 NDCKVVSVAIk-dNRMWYRVETTTss----qHKRFLCRYYQDFYQLHCQLLDEFRRLSLd-------------------- 246 Kluyveromyces l...
XP_718866    334 FEIDQIFYNKs-nKSFWYKIKYYDnl---nhQIIYLGKFYKDFYQLHDGIISQYNNNNNnnnnnnnanninnkntndlsi 409 Candida albican...
XP_462203    131 KSASISNVLLl-dNRYWYRLDITYsd----nSKTYVPRYYEDFYNLHGSLLNMVGKNIDtn-----------------gi 188 Debaryomyces ha...
XP_001382713 168 VSACVAKVYSdstNKYWYRTRMTMst----nEDIYVGKCYQQFYKLHTALVSKYPEETLp-------------------- 223 Pichia stipitis...
XP_447177    200 TKCEVESIELk-dNRLEYTVRIQDse----gNTKVKHIYYSAFYKLHTKLMAGLKENMSl-------------------- 254 Candida glabrat...
XP_001646683 176 EDVSVQSCKLisvDIVEKRVQYTLnlrlannGENIVHRYYGQFYKLHTKLLSIGSNYNEi-------------------- 235 Vanderwaltozyma...
EDK40340     134 VNCSISNCLSf-dNRYWYRLDVTYsd----hSQSYICRYYQDFYELHIALVQCKNSLAAtsis-------------dpke 195 Pichia guillier...
XP_001382712 130 TSVSISNFLLs-eQRYWYRVDVVYsd----hSKAYLCRYYQDFYNLHIKLLGLMAASSQiv-----------------se 187 Pichia stipitis...
Feature 1                ##                   #                                  
2V6V_A        83 KRIXPYIPGPVPyv--------tNSITKKRKEDLNIYVADLVNLp---dYISRSEXVHSLFVVL 135 baker's yeast
NP_986354    191 LNQLPKLPAPAGnk-------lkNSSAEIRMESFNKYLRELFESthipgHVRDSLVVDQWLAPR 247 Ashbya gossypii ATCC 10895
XP_454397    247 RKHLPNLPKPMAns-------ksNESVHSRLNSFTAYLEVLLSSehiptDFKQSIFYDQWLCPK 303 Kluyveromyces lactis NRRL Y-1140
XP_718866    410 KLPPPILRNSLYhspnvkfdknlIENSYKLLIRLNDYLNQLINHwp--nILNECPKFHDFIYNC 471 Candida albicans SC5314
XP_462203    189 SPSLPKLPEPIPptass-qskdlIVMLLKRCNDLSIYTNRLFNDk----RFQRSKALYEWLDVS 247 Debaryomyces hansenii CBS767
XP_001382713 224 KLPLPVRSSSVSgnkry-stlkyDRKFLKDLNALSEGLNSYFNNlllirSIQTSSEVYSFIFDS 286 Pichia stipitis CBS 6054
XP_447177    255 PNIPAPSSNYAQek-------vdESTKLDRLQMFNTYLTYLIDVi---yDIKSPLHLQDTFTNW 308 Candida glabrata CBS 138
XP_001646683 236 KLPSLPHPLPFTgsl-----ehnSTLINDRVQEFNDYLANLLDNi----LNSSSIVLKDLINNW 290 Vanderwaltozyma polyspora DSM 7...
EDK40340     196 TIQLPKLPEPIPstcid-dkhqlMSLLLKRCNDLHVYINKLILNk----NYQTCETFMNWLDLD 254 Pichia guilliermondii ATCC 6260
XP_001382712 188 ELKLPKLPEPLPtnhdv-edssqITSLLKRCNALNIYVNKLILNk----YYQTSQVLLNWLDLN 246 Pichia stipitis CBS 6054

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap