Conserved Protein Domain Family
PX_NoxO1

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cd06889: PX_NoxO1 
The phosphoinositide binding Phox Homology domain of Nox Organizing protein 1
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Nox Organizing protein 1 (NoxO1) is a critical regulator of enzyme kinetics of the nonphagocytic NADPH oxidase Nox1, which catalyzes the transfer of electrons from NADPH to molecular oxygen to form superoxide. Nox1 is expressed in colon, stomach, uterus, prostate, and vascular smooth muscle cells. NoxO1, a homolog of the p47phox subunit of phagocytic NADPH oxidase, is involved in targeting activator subunits (such as NoxA1) to Nox1. It is co-localized with Nox1 in the membranes of resting cells and directs the subcellular localization of Nox1. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of NoxO1 preferentially binds phosphatidylinositol-3,5-bisphosphate [PI(3,5)P2], PI5P, and PI4P.
Statistics
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PSSM-Id: 132799
View PSSM: cd06889
Aligned: 7 rows
Threshold Bit Score: 188.753
Threshold Setting Gi: 41281811
Created: 11-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                  ###                               ##   
NP_751907       3 GPRYPVSVQGAALvq---ikRLQTFAFSVRWSDGSDTFVRRSWDEFRQLKKTLKETFPVEAGLLRRSDRvLPKLLDAPLl 79   human
XP_001366465    3 GPRELVLVRGMGFvp---grRSQIFIFSVLWSDGSDNFIYRTFQEFRQFHKRLKDAFPLEAGLLKSSDCvLPRFPDAFIl 79   gray short-ta...
XP_414847       1 MSRFPVDVKAVGLmq---crKQKKYMMFVSWSDQNNILIYRTLEEFKRFHKELKRKFPIESGSLRRSDRtIPRFKDINGk 77   chicken
AAI61749       16 SSRHPVEVQGIGLmh---hgSHKTYMFSVLWSDHNKVLIYRTMEEFKKFQKELKMKFPLEAGAMKKSERtIPKLKDALRg 92   western clawe...
NP_001071052    3 EQRHPVNIQLLGVmq---keMTKLYMTTVLWSDGNEITVYRSLEDFKKMHRQLKKKFPPSNPFKRSARI-VPEFKEVRVq 78   zebrafish
AAI55849       19 DPRFPVDVHLIGVmk---keKDKKYMTSVLWSDQSELIVYRSLRDFRTLHRQLKKKFPVEKPYRKEDRV-LPRFGAQVMk 94   zebrafish
XP_002225545   89 SGRRLVAVQMVGVekrysprRHHVYIVSELWSDGSRLVVYRSYRQFRALHKQLKEMFPVEAGQFRGEERvLPKFPNKPSs 168  Florida lancelet
Feature 1                       #                               
NP_751907      80 grv---grtSRGLARLQLLETYSRRLLATaeRVARSPTITGFFAPQ 122  human
XP_001366465   80 lps---rrtRRGLARLKMLEAYSQELLGApeTVSRSEVVTGFFQTQ 122  gray short-tailed opossum
XP_414847      78 qkk--sgkiNRSLERLKLLETYTQELLKVdaKISQGEDVIQFFKAQ 121  chicken
AAI61749       93 gsk--rkdsRKYLERLRKLETYCQALLRLdaKISQCPLLVQFFTLH 136  western clawed frog
NP_001071052   79 kslq-kwsgSKSVLRMKALEEYCGQLLKSdpQVCRSSELIQFLLPK 123  zebrafish
AAI55849       95 stfq-lkglDKSVSRLRNLEKYCSSLLQCdtTVSNSREVIQFFLPT 139  zebrafish
XP_002225545  169 pglgrkfsvGRFACVQKDLQEYCQDLLRLdsKIVLSPLFQGFFAPS 214  Florida lancelet

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