Conserved Protein Domain Family
PX_SNX15_like

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cd06881: PX_SNX15_like 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 15-like proteins
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Members of this subfamily have similarity to sorting nexin 15 (SNX15), which contains an N-terminal PX domain and a C-terminal Microtubule Interacting and Trafficking (MIT) domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNX15 plays a role in protein trafficking processes in the endocytic pathway and the trans-Golgi network. The PX domain of SNX15 interacts with the PDGF receptor and is responsible for the membrane association of the protein. Other members of this subfamily contain an additional C-terminal kinase domain, similar to human RPK118, which binds sphingosine kinase and the antioxidant peroxiredoxin-3 (PRDX3). RPK118 may be involved in the transport of proteins such as PRDX3 from the cytoplasm to its site of function in the mitochondria.
Statistics
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PSSM-Id: 132791
View PSSM: cd06881
Aligned: 11 rows
Threshold Bit Score: 161.719
Threshold Setting Gi: 32566063
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                           ###                           
NP_001090475   11 YERRYNVTDTRTnp---kGYTEYKITAQf---ISKKNPQDVk--EIVVWKRYSDLKKLHGELSYTHRnlfq-rcqEFPPF 81   African clawe...
XP_001659021   10 WVHKFSITETRRh----kGYTIYKITSIv---FPRYLPEALs--NVTLWKRFSDVKKLYKDLVRKHKerh--lagTVPQL 78   yellow fever ...
Q9VEA9          8 WIHSFTVTDTQEhk---gGYTIYKITSIv---FPRSVPQALt--CLVVWKRFHDVKRLHRELSRRHKslq--lpgKLPVP 77   fruit fly
XP_001600691    8 WVRRFVISETSRhk---kGFTVYKVTSMm---FLKTSPDVVs--KVSIWKRYSDFKKLYSTMQSLHStmq--ikePFPPF 77   jewel wasp
XP_001624368    8 WTRIFDVSNPRKsy---aGHTLYRVASKv---FPKDFPEAVt--EVVVWKRYSDFKKLHRELLHIYNqqk--hstSFPTF 77   starlet sea a...
XP_001896487   65 WDVHLNIVDTKNvstfftSHTEYEMALCa--iPCNSVAADIr--FFDMWTRFRDVKRLWEQLGDLHKrlh--lhgSFPQF 138  agent of lymp...
EDV29186        8 LSREFAVGKTRLss---qGYTEYQIEYQ----VENQSDEGQl-mQKSKWCRFREIKKLHRDLQTLYTqiatkdsgSFPKF 79   Trichoplax ad...
XP_002213884   12 WIRWFDVSDPQMhe---kGYTIYKVTLKv---RSRLTAEVVs--NVVVWRRYNDFKKLHKALSARYKhla--rsePFPAF 81   Florida lancelet
XP_002119630    7 NNVTYQVVSAQNhn---aGFTLYKILIKe---YKTSGPLDHv-tESIVWKRYNDFKTLHHELFVLHRely--lpgRFPFF 77   Ciona intesti...
NP_506082      26 WSCSSTVTSISEekhltgSHTVYTVEIEcnpvASHLVANDVsrrTFVLSTRYKEMTKLHAAISKLHKqly--lrgTFPVF 103  nematode
Q96S38         11 LARFYTVTEPQRhp---rGYTVYKVTARv---VSRRNPEDVq--EIIVWKRYSDFKKLHKELWQIHKnlfr-hseLFPPF 81   human
Feature 1          ##             #                              
NP_001090475   82 PRAQVFGRFEAAVIEERRQATEEMLQFTVNIpALYNSPQLKEFFREG 128  African clawed frog
XP_001659021   79 TEKTYFKRFDPRVIEERKRYILELLDFAGAEpVLYQSHAFVKFFERG 125  yellow fever mosquito
Q9VEA9         78 TDSTYFKRFDAAVIQRRKEYILQLLDFAAQHpALYKCSTFTQFFQET 124  fruit fly
XP_001600691   78 PKPKFFGRFEAEVIEERRECALKLLEFIGRHnALFTSDAFVKFFETD 124  jewel wasp
XP_001624368   78 PKAKFFGRFDEGVIEDRRQHARELLKFVGNVsSLFTSAPFVKFFEAT 124  starlet sea anemone
XP_001896487  139 AEGVIFGNQSAQVVGKRTLSITTFMNYILNHpVLRQSKALQDYFDKA 185  agent of lymphatic filariasis
EDV29186       80 PRGSYFGRFDEETINSRLIAIRNLLLFCSKIdYLYGSVHFQQFFMED 126  Trichoplax adhaerens
XP_002213884   82 AKAKIFGRFDEAVVEERRQSALELLEFVGKIpYLASSSILQTFFEVR 128  Florida lancelet
XP_002119630   78 VKPKLFGRFDQKVVDERMKCAQELLDFANQHkALYRSSSFKQFFVHA 124  Ciona intestinalis
NP_506082     104 PPGKLIGSSDPSVIQERRHAIEACLNFVFDSeVLRKSRLLHEFVEKA 150  nematode
Q96S38         82 AKGIVFGRFDETVIEERRQCAEDLLQFSANIpALYNSKQLEDFFKGG 128  human

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