Conserved Protein Domain Family
PX_SNX25

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cd06878: PX_SNX25 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 25
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. The function of SNX25 is not yet known. It has been found in exosomes from human malignant pleural effusions. SNX25 shows the same domain architecture as SNX13 and SNX14, containing an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs.
Statistics
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PSSM-Id: 132788
View PSSM: cd06878
Aligned: 12 rows
Threshold Bit Score: 172.944
Threshold Setting Gi: 115689651
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       ##
XP_420506     503 WWCENLGMWKAFIISGEv-----lEENGEQVPCYSVLVSlqevg-----------------------gaetkNWTVPRKL 554  chicken
CAG00678      571 WWCENLGHWRANITAAEa-----tEEGGEMVACYSVCVSlvege-----------------------estnsRWNVNRKL 622  spotted green...
XP_001658823  613 VWGEYLGKWRAIVESVDf------SDDKEPQPQFMIVVQvdevndcgkedv---------atamdaadsistGWVVVRSL 677  yellow fever ...
XP_001630050  497 RWCENIGNWNVQIEGVEw-----nNEDDKWIPLYVIVVHcspinlpispsg---------aspdrlpgatseGWVVTRKF 562  starlet sea a...
EDV24614      620 LWCDLMGQWRTSISNAHv-----vVEGDGYVPVYVILVErassgredsn--------------dketrtisdCWAVTRTL 680  Trichoplax ad...
XP_002217468  469 QWWDMIGRWRANICNAEf-----iVNEEKIEPVFVIVVHisgaias-------------------tdestttGWVVMRNL 524  Florida lancelet
AAL89962      645 AWTEHLGKWKATIQSVE-------VSDEKESLQFMILVHvdedinapvqptsskngdsghanlrkrpsgissGWVVMRSL 717  fruit fly
XP_002120443  574 AWWEHMGSWAANVQDAYcqqdsseGSLNGGGPLYTIVVQcsgvsly-------------------eatddvsGWVVTRCL 634  Ciona intesti...
XP_788218     561 RIEEKLSNKSQALANLKst----hGVDSKMEQLLEIEIEklklqkkrvelyieq----tdlwtehidkiptsGWVVSRRL 632  purple urchin
BAG53100      374 WWCENLGMWKASITSGEv-----tEENGEQLPCYFVMVSlqevg-----------------------gvetkNWTVPRRL 425  human
XP_001123040  593 MWAEHLGHWKAEVQSAE-------IPDNGESPQFVLVVHmrenedn--------------------nesiysGWVVLRKL 645  honey bee
EEB09909      568 VWEEHLGKWRVVVQSAE-------VSADKECPHFVLVVHvledeln--------------------ydaistGWVVLRKL 620  human body louse
Feature 1         #                          ##             #                              
XP_420506     555 SEFQNLHRKLSECFPslkkvQLPSLSKLPFKSi--DQKFMEKSKNQLNNFLQKLLSDeRLCQSEALYAFLSPS 625  chicken
CAG00678      623 PEFQMLHRKLTECFPslkklQLPSLSKLPFKSi--DQKFLDKSKTQLNAFLQRLLTDeRLCQSEALYAFLSPS 693  spotted green puffer...
XP_001658823  678 AQFHDLHRKLRPMCSdlktiDLPSNNAFKLFLlknDKSLLEKSKQQIQKYLNFILEDdHLNQSEALYEFLSPS 750  yellow fever mosquito
XP_001630050  563 NDFKILHAKLKECSSkl-pnELPAPAKKWYKSl--DDEFLNKSRLALEQYLQSLLADeTLCLSEELYSFLSPS 632  starlet sea anemone
EDV24614      681 NEFQELHHTLTDCYPwl-rkNFPILSKKWTKSi--AQTTLDRIKGLLENYLQGLILHeKLCQSDVLYLFLCPS 750  Trichoplax adhaerens
XP_002217468  525 SDFHALHKRLKEYRSwl--rEKDLPSKRWFRAt--DKAFLDKARTMLEAYLNEVLADdTLCQSQVLFSFLSPS 593  Florida lancelet
AAL89962      718 NQVHELQRKLRHVSSnlkaiDLPTNFKFFFLKt--DRHGQEKAKSQIQKFLNFILEDdHLNGSEAIYTFLSPS 788  fruit fly
XP_002120443  635 SDFHNLHHSLKEYSSwlrkqSLPKLPMFRMRGv--SEKFLLESKSTLNTYLHNILKDeILRCSQIVYSFLSPG 705  Ciona intestinalis
XP_788218     633 GEFHDLQNRLKECSHwlakvKLPQNNKKLFNRe-tMNEFLERSKNELQKHLTAILGDaTLRHSEALYSFLIPK 704  purple urchin
BAG53100      426 SEFQNLHRKLSECVPslkkvQLPSLSKLPFKSi--DQKFMEKSKNQLNKFLQNLLSDeRLCQSEALYAFLSPS 496  human
XP_001123040  646 QDFQDLHRKLRQLCSnvknlDLPSQPLKFFGKs--DKNTLDKAKIQIQKYLNFVLEDeKLNQSEALYTFLSPS 716  honey bee
EEB09909      621 SEFHELHKKLSQVWNgvknlELPSQSPKFLFGkhsDKASLDKAKNQIQKYLEFVLQDeRLNTSEAIYTFLSPS 693  human body louse

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