Conserved Protein Domain Family
PX_MDM1p

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cd06876: PX_MDM1p 
The phosphoinositide binding Phox Homology domain of yeast MDM1p
The PX domain is a phosphoinositide binding (PI) module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Yeast MDM1p is a filament-like protein localized in punctate structures distributed throughout the cytoplasm. It plays an important role in nuclear and mitochondrial transmission to daughter buds. Members of this subfamily show similar domain architectures as some sorting nexins (SNXs). Some members are similar to SNX19 in that they contain an N-terminal PXA domain, a central PX domain, and a C-terminal domain that is conserved in some SNXs. Others are similar to SNX13 and SNX14, which also harbor these three domains as well as a regulator of G protein signaling (RGS) domain in between the PXA and PX domains. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.
Statistics
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PSSM-Id: 132786
View PSSM: cd06876
Aligned: 13 rows
Threshold Bit Score: 170.953
Threshold Setting Gi: 170085631
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
XP_453155     755 ELLKQQHMVHENANILYgr-aKLCIKSYLTDVsledgkETVYYLINVQHIfnn--------------------------- 806  Kluyveromyces...
CAA89114       79 ELLKQQYMVQENGNSLFrk-tKIYIRSYFSENssnglkEITYYIINIHHFnng--------------------------- 130  baker's yeast
XP_718232     664 ELQKQQFIVQENDNSLFgk-sRVCIQSYINDNekn-grEFTLYIIEVQKFsnenp------------------------n 717  Candida albic...
NP_985144     785 ELLKQQYTVLENSNSLFgr-tKVSIKNYISELspsdgkDVIYYIINVNHIngn--------------------------- 836  Ashbya gossyp...
EDK37335      718 ELQKQQYMVQENENSLYgk-sRVQIQSYISGSek--gkEYILYIVEVQRFlsddp------------------------t 770  Pichia guilli...
XP_447991     715 DVARQLLLIQESSNALYgt-tEVSISSFYEDVdeggkkLITFYLVNVKRQngd--------------------------- 766  Candida glabr...
XP_001643042  739 ELLHQRYTIQDNTNRLFqk-sDVFVRSHFIDYqtengpEIVYYLINIKFDyri--------------------------- 790  Vanderwaltozy...
CAF06163      871 ELQRQQYVIQESDNSLYgr-sTIKIKNIHVGReed-gkEFAMYAIEVLRNagek-------------------------- 922  Neurospora cr...
XP_504887     984 EMQRQQYIVQETDNSLYgr-sAIAIQNAVSSHdkm-gnEFVVYLVEVTRHgddsdk----------------------eg 1039 Yarrowia lipo...
XP_001874039  839 QFQKQQYEQQESANRLLsdrtKVAIVNSAVADed--gkSVVRYLVEVQQLandg-------------------------- 890  Laccaria bico...
XP_759284     894 RWQRSQLEQQMAATQLIpgqtRVSIQDTMISVdqe-gkEYAVYLIEIGILdqdaskpskpsgqskgrgvgaptaptaptv 972  Ustilago mayd...
NP_587991     680 ERQRDQYMSQEEDSKLFnr-sRVSIDSFKISKeen-tpDFAVYTIRIERLengh-------------------------- 731  fission yeast
XP_002175340  671 ERQRAQYLSQEEDSKLYnr-tRITIDNYKQSRddd-gnEFAVYVMRVERIengd-------------------------- 722  Schizosacchar...
Feature 1                   ###                            ##                #                    
XP_453155     807 -qlvTWDIPRRFSEFYRLNSYLKKKYPgvvsylqnSNGFPERVKMSlkyhvsktLLYEERRKRLEYYMQELLKIrEVCQD 885  Kluyveromyces...
CAA89114      131 -qvsSWDMARRYNEFFELNTYLKKNFRdlm--rqlQDLFPSKVKMSlkyhvtktLLYEERKQKLEKYLRELLSIsEICED 207  baker's yeast
XP_718232     718 ivkaGWVVARRFSQFYGLHEYLKSKYHrv-----sYLKFPKKSISVlkf--qqkQVAEIRMKQLEDYLQELIKIqEVCSD 790  Candida albic...
NP_985144     837 -qvtSWEIPRRYSEFYKLNAYLKNTYGslvghlqrKHIFPQKVKISlkyhvsksLLYEERRLKLENYLRMLLLItDVCQD 915  Ashbya gossyp...
EDK37335      771 vpsaGWVVARRFSQFHHLHVYLRKRYPpv-----eGLKFPKKAVSMlkf--qqrQLVEMRKAALEEYLCQLLKLpDVCGD 843  Pichia guilli...
XP_447991     767 -kstSWEVSRRFNEFAVLYKYLHANYSkkl--kklDLSFPSKIPITenyniakvDICNERRIKFELFLNQLLQIqEICED 843  Candida glabr...
XP_001643042  791 -nqkSWKIARRFSEFVKLYSYLNDKHGnlfkklncKLDFPQRINISfrfhvsktLLFEDRQNKLEAFLRKLIVIpEICDD 869  Vanderwaltozy...
CAF06163      923 mpaaTWTVTRRYSEFLELHQKLRSQYSsv-----rNLDFPRRRMVMkl----qnDFLQKRRVALEKYLNQLLLLpDVCHS 993  Neurospora cr...
XP_504887    1040 assaTWIVARRYSHFLQLHQHLIRSFPyi-----sRIPFPKKKVVIkfn---qkSFVDSRKVQLEAYLRELLKMpEICNS 1111 Yarrowia lipo...
XP_001874039  891 sfssGWVVARRYNEFLTMHNKLRERYAli-----kNLDFPGKRLVPsl----sgNFFDNRKNALEKYLQNVVAIpIVCES 961  Laccaria bico...
XP_759284     973 ptarGWIVGRRYSEFWSLHQALKERFAavr---alESDFPGKRLVGlv----haPFVDARKAALERYLQALVKLkVACES 1045 Ustilago mayd...
NP_587991     732 -vrsGWMVARRYREFAELHKQLKQTYPgv-----rSLKFPQKSIITsl----nkNVLEYRRGALEEYLQSLFRMpEVCDS 801  fission yeast
XP_002175340  723 -vssGWVVARRYREFNELHKQLKRECPqv-----gLLKFPQKTIISspl---stQTLEFRRKALEQYLRSILEIaEVCDG 793  Schizosacchar...
Feature 1                  
XP_453155     886 EYFRKYLTD 894  Kluyveromyces lactis NRRL Y-1140
CAA89114      208 NIFRRFLTD 216  baker's yeast
XP_718232     791 RAFRSFLSS 799  Candida albicans SC5314
NP_985144     916 TTFRRFLTD 924  Ashbya gossypii ATCC 10895
EDK37335      844 RVFRSFLSS 852  Pichia guilliermondii ATCC 6260
XP_447991     844 DLFRTFLTN 852  Candida glabrata CBS 138
XP_001643042  870 EIFRKFLTD 878  Vanderwaltozyma polyspora DSM 70294
CAF06163      994 RELRTFLSQ 1002 Neurospora crassa
XP_504887    1112 VAYRAFLSS 1120 Yarrowia lipolytica CLIB122
XP_001874039  962 PELRAFLSR 970  Laccaria bicolor S238N-H82
XP_759284    1046 EELRIFLSQ 1054 Ustilago maydis 521
NP_587991     802 KMLRMFLSQ 810  fission yeast
XP_002175340  794 KTFRVFLSQ 802  Schizosaccharomyces japonicus yFS275

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