2V14


Conserved Protein Domain Family
PX_KIF16B_SNX23

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cd06874: PX_KIF16B_SNX23 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of KIF16B kinesin or Sorting Nexin 23
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. KIF16B, also called sorting nexin 23 (SNX23), is a family-3 kinesin which harbors an N-terminal kinesin motor domain containing ATP and microtubule binding sites, a ForkHead Associated (FHA) domain, and a C-terminal PX domain. The PX domain of KIF16B binds to phosphatidylinositol-3-phosphate (PI3P) in early endosomes and plays a role in the transport of early endosomes to the plus end of microtubules. By regulating early endosome plus end motility, KIF16B modulates the balance between recycling and degradation of receptors. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.
Statistics
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PSSM-Id: 132784
View PSSM: cd06874
Aligned: 8 rows
Threshold Bit Score: 192.211
Threshold Setting Gi: 24650642
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                             ###                        ##             # 
2V14_A          6 IKISIPRYVLCGQgkDAHFEFEVKITVldETWTVFRRYSRFREXHKTLKLKYAe-lAALEFPPKKLFgNKDER-VIAERR 83   human
NP_524532    1131 HFITIPSFVMRGAgkQTHYEYEVRIALpdGKLNILRRYSRFRELHLCMKHCYGakiSALPFPRRELF-ASNSEpVAKHRR 1209 fruit fly
XP_419330    1341 IKISIPRYVLCGQgkDEHYEFEIKITVldETWTVFRRYSRFREMHRTLKLKYPe-vATLEFPPKKLFgNKDER-VIAERR 1418 chicken
XP_001625664    6 IQVQIPRYILRGQgrDSYHVFEVKVTIagDTWSVYRRYRKFRELHNTMKRLYIe-vGELDFPHRRFFgNRAEE-FVRARR 83   starlet sea a...
XP_973885    1012 SVVTISSYCIRGAgtKTHYEYEVRVCTsdDRWSILRRYSRFRDLHIAMKARYGdkvASIPFPAKQLF-ANSET-VAKSRK 1089 red flour beetle
XP_002222310 1106 IKISIPRFVMRGYgkEEHFEFEVKTDVlgEVWTIFRRYSKFRELHEMMKGKYPe-vNALEFPPKKLFgNKSQK-LAEERR 1183 Florida lancelet
XP_789924    1273 IQITLPRFMQRGHgwDSYFVFELHLKVlnESWIVFRRYRRFRELHDYMRRKYKe-vNALQFPPRRLFgNKSER-VVLRRR 1350 purple urchin
CAX14446       18 VKICIPRYVLCGQgkDEHFEFEVKITVldETWTVFRRYSRFREMHKSMKLKYPe-lAALDFPPKKIFgNRDER-TIAERR 95   zebrafish
Feature 1                                                            
2V14_A         84 SHLEKYLRDFFSVXLQsatsplh-inkvgltLSKHTICEFSPFFKKGVFDY 133  human
NP_524532    1210 RLLELYLRRLFVVCSKipqcpiy-egpggtgLTRASLVQLSSFFKKGLFEN 1259 fruit fly
XP_419330    1419 SHLETYLRSFFTAMLQspssplh-idkvgstLSKHTICEFSPFFRKGVFDY 1468 chicken
XP_001625664   84 AQLETYLKSFISLCASlhdcpis--afsgrlIAKRDLCDFAPFFKQGIFEQ 132  starlet sea anemone
XP_973885    1090 RQLEFYLRRLIETCKNlpacpl----aygdtVTKAALISFSPFFRKGVFEN 1136 red flour beetle
XP_002222310 1184 VQLEAYLNNFIAVCLRnkasply--prkdkpFSKRTLCDFAPFFRKGIFES 1232 Florida lancelet
XP_789924    1351 MELERYLQNLMHVCMKipkcpia--pgpgrvLSKQVVCEWSPFFRKGAFDI 1399 purple urchin
CAX14446       96 NQLEQYLRNFFRVMMStsssspistdefglnLSKHAVWDISPFFKKGVFDY 146  zebrafish

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