Conserved Protein Domain Family
PX_SNX13

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cd06873: PX_SNX13 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 13
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX13, also called RGS-PX1, contains an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs. It specifically binds to the stimulatory subunit of the heterotrimeric G protein G(alpha)s, serving as its GTPase activating protein, through the RGS domain. It preferentially binds phosphatidylinositol-3-phosphate (PI3P) through the PX domain and is localized in early endosomes. SNX13 is involved in endosomal sorting of EGFR into multivesicular bodies (MVB) for delivery to the lysosome.
Statistics
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PSSM-Id: 132783
View PSSM: cd06873
Aligned: 15 rows
Threshold Bit Score: 172.453
Threshold Setting Gi: 190586172
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                         ###             
NP_001103225  557 ETVQLHAYISDTGVCn------------------dHGKTYALYAITVHRRnan--seETWKTYRRYSDFHDFHMRITEQF 616  chicken
Q9Y5W8        557 DSVQLHAYISDTVYAdydpyav-------agvcndHGKTYALYAITVHRRnln--seEMWKTYRRYSDFHDFHMRITEQF 627  human
AAO15004      602 ESMHIHAFISDTGVCn------------------dHGKTYALYAITVYRRnqd-gseECWKTYRRYSDFHDFHMRITEQF 662  torafugu
XP_792403     712 NKPHIMAYITHASIVk------------------eKGKSYAVYAIHVTRTdsq-gvtEIWDVFRRYSDFHDLHMCLAEKY 772  purple urchin
XP_001629685  498 DDLRLTASINQIGLCk------------------dNGKTYAVYAISVFRAtqe--gtNSWTIYRRYSDFDDLHMHLKNKF 557  starlet sea a...
XP_001898575  560 DGLKCTHVGMHVVVEtlgi-------------gqqGKQSFALYNVRVARIdrsgkqsSTWNVLRRYSDFHTLNSLIQSRF 626  agent of lymp...
EDV26225      549 DGDHSTSQQTNVSLNgvsftvavttigvtknastgTGKTYAVYAISVTKHspt--gdESWEVYRRYSDFHDLHMQLKEKL 626  Trichoplax ad...
XP_001945373  555 DKSILSADIIETGLVh------------------eGGKTFGVYAVSITRVyms-gkeENWHVYKRYSDFYDLYQRVKDKY 615  pea aphid
XP_002205219  666 DQIKLTAKIMQTGICk------------------eHGKQYALYAINVRKEtpi--eeEVWDTFRRYSDFHDLHMRLKDKF 725  Florida lancelet
XP_002125747  584 EVNKLTAVITQTGICk------------------eHGKTYAMFAVTVTRHwgn-grqESWDTFRRYREFYDLHLSLKENG 644  Ciona intesti...
Feature 1                    ##             #                                  
NP_001103225  617 EnlanILKLPGKKTF-NNMDREFLEKRKKDLNAYLQLLLNPemmkaSPALAHYVYDFLENK 676  chicken
Q9Y5W8        628 EslssILKLPGKKTF-NNMDRDFLEKRKKDLNAYLQLLLAPemmkaSPALAHYVYDFLENK 687  human
AAO15004      663 EnlasILKLPGKKTF-NNMDRDFLEKRKKDLNAYLQLLLNPemvkaCPTLIPYVYDFLENK 722  torafugu
XP_792403     773 Esl-rTLLLPAKRAF-KNTNKSFLEKRAKSLTQYIQTLLNQdvlttNPGLQSIICNFLEPG 831  purple urchin
XP_001629685  558 Gpl-pNLLLPGKRTF-RNMDKDFLEKRRAALDSYIQTLLSTdfldkYPGMFEVVANFLEHG 616  starlet sea anemone
XP_001898575  627 Pkl-sNLCFPGKKTF-NNLDSRFLEKRTKALNSYMMSILQPsvleaNADLETLIFDFLSMK 685  agent of lymphatic filariasis
EDV26225      627 Ggk-lSISLPGKRTFgNNLDKAFLESRRRKLDLYLKGIVDPefmnmHKSLIVIIASFLEPG 686  Trichoplax adhaerens
XP_001945373  616 Sel-gKLSFPAKKTF-HNADRRVLEKRMMMLNQFIHNLLKSstvanYPGIQTMVEQFLEQR 674  pea aphid
XP_002205219  726 Dsl-yALKLPAKKTF-KNLNKEFLEKRRKELNQYLQTLLCEdvlqnNPGMKEAMLDFLENK 784  Florida lancelet
XP_002125747  645 Snl-sTIRFPGRTFF-KDLKEDFLERRRTELNQYLSSVLSLe---hCGKAMECLHTFLDAK 700  Ciona intestinalis

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