Conserved Protein Domain Family
PX_Atg24p

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cd06863: PX_Atg24p 
The phosphoinositide binding Phox Homology domain of yeast Atg24p, an autophagic degradation protein
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. The yeast Atg24p is a sorting nexin (SNX) which is involved in membrane fusion events at the vacuolar surface during pexophagy. This is facilitated via binding of Atg24p to phosphatidylinositol 3-phosphate (PI3P) through its PX domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.
Statistics
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PSSM-Id: 132773
View PSSM: cd06863
Aligned: 14 rows
Threshold Bit Score: 167.465
Threshold Setting Gi: 6322424
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                ###     
AAZ14926      98 LITAVRDPVKEla----------------eTKDAYVSYLVSAKTNLpif--------------stpSPSSRRRYQDFKFL 147 Coprinellus dis...
ABO31320      72 LECRVDSPLKEnd----------------gTKDAYISYLVTTHTDFksf--------------qkpDFTVRRRFTDFYFL 121 Penicillium chr...
XP_760067    108 LMVQVLEPRKEle----------------gQKDAFISYGIRAETNLahf--------------srtYMAARRRFNDFTFL 157 Ustilago maydis...
XP_001732696  15 LRVLVLDPRKEthgaqqqnrtndthapttrGSQTFVSYGVRAETNLphf--------------srtYMITRKRFQDFVFL 80  Malassezia glob...
NP_595884      2 LKCTIKNEQIEtl----------------rSGDTFVSYEIETESDLpvf--------------edkKFSVRRRYKDFEML 51  fission yeast
XP_456420    117 INVVVTSPLRDld----------------sSSKPYISYLLTTATNHpsvmklstv--kkeqgkevvEITARRRYGDFRFL 178 Debaryomyces ha...
EDK40859      76 TTTEVTSPTRDtd----------------aAQKPFVTYLVTTVTNDpsiirlcstlkkvsedndmqSVAVRRRYGDFKFL 139 Pichia guillier...
XP_002172388   3 FICRVADPQFEkp---------------lsGGNVYISYLILTKTELhtg--------------qenELHARRRFRDFTLL 53  Schizosaccharom...
NP_012498     28 FEIIVSDPQKRtgd--------------pgSSSGYVSYQISTKTNNtsfydn--------rgdpesIIVVHRRYSDLLLL 85  baker's yeast
XP_452689     17 LSVLVSDPQKQk-----------------tHSQTYITYQVSTKIDGkd-----------------ePNCVVRRYNDFVLL 62  Kluyveromyces l...
Feature 1                          ##                 #                              
AAZ14926     148 REHLVRDFPACVVPALPDKHRLEYITGDRFSPEFMERRRLDLHRFLERIARHpILQRSTLLRAFFEST 215 Coprinellus disseminatus
ABO31320     122 YKTLYREYPACAVPPLPDKHKMEYVRGDRFGPEFTTRRAWSLHRFLKRLTLHpVLRRAPLLTIFLESP 189 Penicillium chrysogenum
XP_760067    158 REGLKRDFPACVVAPLPDKHRLEYLTGDRFSAEFIERRTQDLQLFLERICRHpTLQRSQLLRSFLEST 225 Ustilago maydis 521
XP_001732696  81 HDALVSDFPACIVPPLPGKHRIGYLTGDRFSSEFIMRRCMELQLFLERVCRHpLLQRAKILQQFLESN 148 Malassezia globosa CBS 7966
NP_595884     52 HNILSHDYNGYAIPPLPRKYTVSSFSGGSLSPIFIARRMQSLQTFLDRCSTHpVISNSMHMYQFLENN 119 fission yeast
XP_456420    179 FDCLSNDHPEVMMPPLPSKLNFKYLTGDTFSTEFVHKRLHSLDRFVRFITCHkVLSQLSIFHLFVSDS 246 Debaryomyces hansenii CBS767
EDK40859     140 HECLTADYPQHLVPPLPSKSNFKYLTGDTFHEEFITKRLQSLNRFIHYTNQHqYLSQSSIFKLFVSDS 207 Pichia guilliermondii ATCC ...
XP_002172388  54 HELLRREYPLHFIPPIPRKHGVKQITGDAFSPDFIPRRLSSLQRFLDRCLEHpVLKSSLHLYQFLEVS 121 Schizosaccharomyces japonic...
NP_012498     86 HDILLNRFPTCIIPPLPDKKVFQYIAGDRFSQRFTQKRCHSLQNFLRRVSLHpDLSQSKVFKTFLVSK 153 baker's yeast
XP_452689     63 HQILINDHPALLVPPLPDKKVLNYLSGDRFSHSFTQKRCRSLQTFMRRLLSHsELSKSRILETFLTST 130 Kluyveromyces lactis NRRL Y...

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