2RAK


Conserved Protein Domain Family
PX_SNX9_18_like

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cd06862: PX_SNX9_18_like 
Click on image for an interactive view with Cn3D
The phosphoinositide binding Phox Homology domain of Sorting Nexins 9 and 18
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX9, SNX18, and similar proteins. They contain an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain. SNX9 is localized to plasma membrane endocytic sites and acts primarily in clathrin-mediated endocytosis, while SNX18 is localized to peripheral endosomal structures, and acts in a trafficking pathway that is clathrin-independent but relies on AP-1 and PACS1.
Statistics
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PSSM-Id: 132772
View PSSM: cd06862
Aligned: 13 rows
Threshold Bit Score: 197.924
Threshold Setting Gi: 190588795
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Structure:2RAK_A; Human SNX9 PX domain binds Phosphatidylinositol-3-Phosphate; defined at 3.5A contacts.
    View structure with Cn3D
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                    ###                  
2RAK_A         47 FDCVVADPRkg-------------------sKMYGLKSYIEYQLTPTn----tNRSVNHRYKHFDWLYERLLVKFGsaIP 103  human
Q96RF0        276 FQCTIDDPTkq-------------------tKFKGMKSYISYKLVPTh----tQVPVHRRYKHFDWLYARLAEKFPv-IS 331  human
Q9NCC3        219 YSVVVASPKke-------------------sKFKGMKTFIAYQLTPSf----nNISVSRRYKHFDWLHERLVDKFCl-IP 274  fruit fly
XP_001641890  189 FTCSVTEPEkk-------------------sKFKGMKSFISYNVVPTn----tMRPVSRRFKHFDWLYNRLVDKYTl-IA 244  starlet sea a...
NP_872090     227 YTCIVDKPKke-------------------sKLKGLKSFIAYSITSSl----tNIQVSRRYKHFDWLHEQLSAKYVl-IP 282  nematode
XP_001744205  129 LSGHVWQPSqepftlev------saqegkesKFGGLKKFTAYQICNTt----tQATVMRRFKHFDWLHQRLVELYPc-IV 197  Monosiga brev...
EDV28817      159 KHIIVVNKNgltwkpaknectyrigstetdsKYAGAKTYTTYKIHPIhg---dDEAIRRRYKQFDWLYGRLVARFPt-IA 234  Trichoplax ad...
XP_001748157  357 GHITIEHCGar-------------------sKYKGLKSYEAFLIKGAv----pGTEAERRHKHFQWLHDRLVDKYSc-LC 412  Monosiga brev...
XP_002210589  240 YKVTVADPKkd-------------------sKLKGLKSFITYQLTPDn----tNQTVSRRYKHFDWLYERLIEKFTt-IA 295  Florida lancelet
XP_002127441  219 YSCSIENPKqq-------------------sKMKGLKKFVCYNLTPSd----tAVTVQRRYKHFDWLYARLVERYPm-VA 274  Ciona intesti...
XP_002233154  173 FSVEIKNPTka-------------------sKLKGMKSFIAYEVTPTh----tNRSVTRRYKHFDWLYNRLVEKYSv-IS 228  Florida lancelet
XP_002124791  263 FTCTVTNPVke-------------------sKFSGLKSFIAYTITPSh----tDVAVLRRFKHFDWLYEQLVRKYGvsIS 319  Ciona intesti...
XP_758695    1006 YHVTSTYPLgdq-----------------sdAVSGSQGCIPYDPLGGphpagaQVTVLRRFTQFEWLHQVLAKHFSa-LL 1067 Ustilago mayd...
Feature 1               #             #                                                    
2RAK_A        104 IPSLPDKQVTGRFEEEFIKMRMERLQAWMTRMCRHPVIS-ESEVFQQFLNFR---DEKEWKTGKRKAERDELA 172  human
Q96RF0        332 VPHLPEKQATGRFEEDFISKRRKGLIWWMNHMASHPVLA-QCDVFQHFLTCPsstDEKAWKQGKRKAEKDEMV 403  human
Q9NCC3        275 VPPLPDKQISGRYEEQFVEHRRVQLQEFVDWVCRHPVIS-KCEVWYHFLTCR---DEKIWKSGKRKAERDPYM 343  fruit fly
XP_001641890  245 IPPLPDKQITGRFGEDFVEKRREKLEKWVGRLCQHPVLS-KSPVVNHFLTCAd--SEKEWKAGKRKAENDKFT 314  starlet sea anemone
NP_872090     283 IPPLPEKQVAGRYEEDLIDHRKHILQLWVNKICRHPVLS-QSEVWLHFISCT---DEKDWKNGKRRAEKDEYI 351  nematode
XP_001744205  198 IPPLPEKQVSGRFSKNFVEYRRQKLERMLNRVALHPVLG-ASSVFRHFLTAT---DKKDWKSGKRQAEGEAKS 266  Monosiga brevicollis...
EDV28817      235 IPKLPEKRIRGRFSEDIIQMRKELLETWLNRLARHPIIN-ESQVFSGFLKKQ---DQIEWKEFKRRMEKDPGN 303  Trichoplax adhaerens
XP_001748157  413 VPPMPGKEYESKFNENHVKKREERLEAWINRVARHPVLGaDVLALKHFLTCSt-gDATRWKQGKRDAEKDRFL 484  Monosiga brevicollis...
XP_002210589  296 VPPLPEKQVTGRYEENFVEGRRQQLQLWVDRMSCHPVVA-QSEVFQFFLQSP---SDKTWKDGKRRAEKDPLV 364  Florida lancelet
XP_002127441  275 IPRLPDKQASGRFEEEFIEGRMARLKLWISYICQHPVLS-RAQIFRNFLTLT---EQKAWKMSKRQAEKDDLV 343  Ciona intestinalis
XP_002233154  229 IPPLPDKQVTGRYEEEFIQHRMAQLQLWMNRMTRHSIVS-RADVFQHFLSCGd--DKNTWKAGKRRAERDNLI 298  Florida lancelet
XP_002124791  320 VPQLPDKQITGRYEDDFIEGRRVQLQTWLCRIALHPVIS-CSDVFGHFVTSKd--DEKAWKQGKRKAEKDEAV 389  Ciona intestinalis
XP_758695    1068 IHPVPEKQYSGRFASDFIETRRADLEMWMSRLVRHPVLR-YSEPLRFFLSCE---DEVDWRSYAANLLRYGCS 1136 Ustilago maydis 521

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