Conserved Protein Domain Family
PX_SNX7_30_like

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cd06860: PX_SNX7_30_like 
The phosphoinositide binding Phox Homology domain of Sorting Nexins 7 and 30
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. This subfamily consists of SNX7, SNX30, and similar proteins. They harbor a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to the sorting nexins SNX1-2, SNX4-6, SNX8, and SNX32. Both domains have been shown to determine the specific membrane-targeting of SNX1. The specific function of the sorting nexins in this subfamily has yet to be elucidated.
Statistics
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PSSM-Id: 132770
View PSSM: cd06860
Aligned: 13 rows
Threshold Bit Score: 187.158
Threshold Setting Gi: 194389930
Created: 10-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                 ###                       ##           
Q9UNH6        32 LFITVDEPESHvttieTFITYRIITKTSRgeFDSSEFEVRRRYQDFLWLKGKLEEAHPTLIIPPLPEKFiv-kGMVERFN 110 human
XP_001952836  52 IQVRVDNPQKIvepleTYITYRVSTKADRtdYPHKEYVIRRRYNDFVWLRQNIAVEYPDRIVPPLPAKHti-lGQLDRYS 130 pea aphid
CAF92635      29 LLISVDEPESHvttieTFITYRVVTKTTRsdFDCSEYQVHRRYQDFLWLRSRLEESSPTLIVHPLPEKFvv-kGLVERFS 107 spotted green p...
EDV22173      14 LIIAVNQPEKHgsgldAYVSYNVVTKTTRtaFDAAEYSVRRRYQDFLWLHTRLTENFPLVIIPPLPEKQv--lKRLDRFT 91  Trichoplax adha...
XP_001604661  79 LQVKVENPQKHvetleTYITFRITTRSTRpeYEEGEYIVRRRYNDFIWLRQKLVESYPAHIIPPMPGKHsl-lAQLDRYS 157 jewel wasp
XP_001638958  27 LFIRVDDPEKHv---gGYVSYNVITKTTRnqFDHPEYSVRRRYQDFLWLRQRMQESYPTHIIPPLPEKHsf-tKHFDRFS 102 starlet sea ane...
XP_784282     75 LFIKVDDPMRHtgkmeSFVSYRVTTKTTRssFDNPEYSVRRRYQDFLWLRQKLAEVQPTHLVPPLPEKQs---MRLDRFA 151 purple urchin
XP_424910     92 LFVTVDDPKKHvctmeTYITYRVTTKTTRaeFDLPEYSVRRRYQDFDWLRNKLEESQPTHLIPPLPEKFvv-kGVVDRFS 170 chicken
EEC00860      82 LIVKVDNPEKHsgtldSYVTFRVTTNTTHsdYDAHEYSVRRRYKDFLWLKQALERSHPGCIIPPLPDRS----VLQHRFS 157 black-legged tick
EEB12625      72 LVVKIDSPEKHfdlldTYITFRVTTKTTRpeFKTNNYCVKRRYNDFVWLRQNLVAEYPTHFVPPLPVKHsl-iEQLDRYG 150 human body louse
XP_002126345  61 LFITVSNPEKHittmeTYITYKITTKTTRsiFDNSEYSVQRRYQDFVWLRNKLEEKHETHLIPPLPSKFim-kGMLDRFN 139 Ciona intestinalis
BAG60481      32 LFITVDEPESHvttieTFITYRIITKTSRgeFDSSEFEVRRRYQDFLWLKGKLEEAHPTLIIPELSSHKkqgpGLLSRMG 111 human
XP_002249919  83 LFVTVDDPEKHitnmeSYITYRVTTKTTRsdYDSHEYVVRRRYQDFLWLRERLEATNPTHLIPPLPEKHs--fRRLDRFS 160 Florida lancelet
Feature 1                #                              
Q9UNH6       111 DD--FIETRRKALHKFLNRIADHPTLTFNEDFKIFLTAQ 147 human
XP_001952836 131 KE--FVTCRMALLERFLSRLVCHPILTEDKHLRVFLTAN 167 pea aphid
CAF92635     108 AD--FIETRRRALQRFLARVAQHPLLSRSQHLRLFLTAP 144 spotted green pufferfish
EDV22173      92 PE--FIHLRQLALEKFLVRVAKHEKLTNCDELKTFLTAK 128 Trichoplax adhaerens
XP_001604661 158 KE--FIIARMKLLHIFLNRMVNHPILSYDKNLHVFLTAK 194 jewel wasp
XP_001638958 103 PE--FLKARELALNKFMTRIADHPVMSFNDHLHVFLTAK 139 starlet sea anemone
XP_784282    152 PE--FLAARRRALHKFLERISEHPVLSFNENLQVFVTAK 188 purple urchin
XP_424910    171 EE--FVETRRKALDKFLKRITDHPVLSFNEHFNVFLTAK 207 chicken
EEC00860     158 QE--FLRFRMLGLHQFLNRVVEHPSLCTEPSLKLFLTAK 194 black-legged tick
EEB12625     151 KE--FVNTRMTGLQSFLFRLVKHPILSCSTGFRIFLTAT 187 human body louse
XP_002126345 140 RQ--FTQTRCRALHNFLDRISRHSVISFDENLQVFLTAK 176 Ciona intestinalis
BAG60481     112 QTvrAVASSMRGVKNRPEEFMEMNNFIELFSQKINLIDK 150 human
XP_002249919 161 PE--FLKTRQAALQKFLSRIVDHPTLSFNENLTIFLTAK 197 Florida lancelet

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