Conserved Protein Domain Family
GT_GPT_archaea

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cd06856: GT_GPT_archaea 
UDP-GlcNAc:dolichol-P GlcNAc-1-P transferase (GPT)-like proteins in archaea. Eukaryotic GPT catalyzes the transfer of GlcNAc-1-P from UDP-GlcNAc to dolichol-P to form GlcNAc-P-P-dolichol. The reaction is the first step in the assembly of dolichol-linked oligosaccharide intermediates and is essential for eukaryotic N-linked glycosylation. Evidence for the existence of the N-glycosylation pathway in archaea has emerged and genes responsible for the pathway have been identified. A glycosyl transferase gene Mv1751 in M. voltae encodes for the enzyme that carries out the first step in the pathway, the attachment of GlcNAc to a dolichol lipid carrier in the membrane. A lethal mutation in the alg7 (GPT) gene in Saccharomyces cerevisiae was successfully complemented with Mv1751, the archaea gene, indicating that eukaryotic and archaeal enzymes may use the same substrates and are evolutionarily closer than the bacterial enzyme, which uses a different substrate.
Statistics
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PSSM-Id: 133466
View PSSM: cd06856
Aligned: 23 rows
Threshold Bit Score: 194.389
Threshold Setting Gi: 18314106
Created: 5-Nov-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Mg++ bindingputative
Feature 1:Mg++ binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                        ##              
YP_843372     30 LGWDMHKPGrpKVPEMGGVPLVFGFVLGVLvyigietfylnsyrytpILAALCTVLMACIIGIMDDIlgwkaglrqWQKP 109 Methanosaeta th...
P96000        34 VGKDINKPDkpEIPLLGGIGIIAGFIAGSFsllltd-----vrservIPAVILSSLLIAFLGLLDDIfnv----rqSVRA 104 Sulfolobus solf...
EDY35579       1 MGKDMNKYDhpEIAEAGGIGVIIGITIGIFiylflkalfgsdthlaeIYATLSAVILAGLIGFSDDIlgwkegipqRYKP 80  Aciduliprofundu...
EDY35516      33 WRDDPNKPYkrEVLTGGGIPVLFALTIVYValgvky-----sqhslfFFGLLITTFMGSFFGFIDDVrdv----kiWKKV 103 Aciduliprofundu...
NP_634120     42 VAKDVYKKGtpTIADRGGTAILLIAMLSLSmnslff------kftstNYVAMIVIALFGLFGVLDDMini----grSSKL 111 Methanosarcina ...
ABZ08607      36 TVKDVNKKGnvMIPRPGGISILIGIVASEItlyff-------fpiseILAIIITSVLGFSVGIIDDRktm----ggWFKP 104 uncultured mari...
NP_560773     32 TSRDVYKGId-GVPRAGGLIAVAASAVGYGvaal---------vaeeAAVVLVISLIIGLLGLLDDLrgv----seYVRV 97  Pyrobaculum aer...
YP_876637     35 SVRDVNKPGrvMVARPGGISIMAGILAAEAvlyaa-------fqsveVLAITATTAGAFAAGLLDDRrvm----sgWFKP 103 Cenarchaeum sym...
YP_919824     38 RRPDAHKPGnpLVAHSGGVILFLGFLLSFSlliafggl--gfsekvkLLAVLATSALCFAVGLLDDKlvl----kgVLKT 111 Thermofilum pen...
YP_001540799  28 LVPDVHKPNkpLIPKLGGLVVLAYVAVSFTllsisrd----ltnsmyYVVFYTTPIVLGVIGIVDDFthv----heFLRV 99  Caldivirga maqu...
Feature 1                                                                                        
YP_843372    110 MFMLFAAMPMMVInaghttmslp-----ligrvdwgilYPLVIIPIGVVGASNAFNMVAGYNGLEAGMGVIIFAALGyag 184 Methanosaeta th...
P96000       105 FLPIFASVPLIVYsvghsiisip-----flgpinfgifYYIIIIPFALTITSNAFNMLEGLNGLGVGMGIIMLSALAyig 179 Sulfolobus solf...
EDY35579      81 ILTTILALPFMTLtlihpyynsf------eswrvplwmYSLLFVPIAIIGTSNAINMVAGYNGLEAGLSSIILLTMAika 154 Aciduliprofundu...
EDY35516     104 PIMLIPPIPFVIInilnplwnnt-----tvlwinwgllYWILIIPVVYMGFSNGANIIAGYDGLEVGIYILILLLYIfig 178 Aciduliprofundu...
NP_634120    112 LIMYYCSYPLIQYathtavtlps------vgdlelgilYLQFIVPTYVLVASNLVNMHSGFNGLSSGLAILVLVSLIiks 185 Methanosarcina ...
ABZ08607     105 VALAFCAAPILLLgaydsnldfp------lfgsvkipsLYLALVVFMIPITGNTFNSIDVLNGVVSGFTTIASFALAisl 178 uncultured mari...
NP_560773     98 LVPVFLAIGLARAvgqmkltv---------pmvglfygATGWFAALALPVMTNAFNMLDPVNGYLPAANIIIGLALAava 168 Pyrobaculum aer...
YP_876637    104 LALAAASAPIIVLgaydphlsfp------lfgsvhipiLYLAVAALMIPITGNTINSIDVLNGVSSGFMVIAGAALTisl 177 Cenarchaeum sym...
YP_919824    112 FLTALSIIPILVValtypaaidwgrpvvpiigrmrmtvIYWVLFPISIAGSANVVNMLDVLNGIVPGTALVAFAALAvig 191 Thermofilum pen...
YP_001540799 100 GASVLYPLIILLAmggklgylnf-----piighfsnpvVIIVLTIAVYGVFSNAINMMDVVNGVVPYSMILTSIIPLays 174 Caldivirga maqu...
Feature 1                                                                                        
YP_843372    185 lvmg--ktsaaaLSIIMLGSLLAFLYFNRYPARVFPGDTMTYSVGALAACVAILGDiekiaVTLFIPYAIDFFMQARGRf 262 Methanosaeta th...
P96000       180 lthtgptyqaglIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFmytalAILYIPYVVEFILKLRTNf 259 Sulfolobus solf...
EDY35579     155 ysms--elwisyMALLAVAALIGFLAFNWYPAKVFPGDSLTYPIGTYIGALAILGNmelfgALLFPLYYTKLILYIKAKk 232 Aciduliprofundu...
EDY35516     179 iiln--nslvlhISIPLMFALLAFELYNLYPSKVLLGNVGSFPIGGLLGIIPFVGHfeivlPIVFLPHLVEFLFKVKYKg 256 Aciduliprofundu...
NP_634120    186 vlis--dveniiSVVAITGATIGFYLYEQYPARIFWGNVGSLTVGAAIGTIIVIQGfiisgFIMLIPHTVNFLMYVYWKi 263 Methanosarcina ...
ABZ08607     179 fvqq--nyeigiACLCLAFASLAFYKYHKLPSRIFPGDSGAVMFGATYGVIAIVGEveviaAVAILPAIINSFLFLSSVk 256 uncultured mari...
NP_560773    169 virg--qadaafLLAIHVAASLALFLYNKYPAKTFNGNVGSYFLGASISTIAVLYDlvaylILTGLPFVINGALIIFSSg 246 Pyrobaculum aer...
YP_876637    178 viig--nyemaaVSMPLPLVALAFYRYHKNPSRIFPGDSGALALGAMYGAIALAGGveiiaAVALLPAVINSFLFLSSMr 255 Cenarchaeum sym...
YP_919824    192 alsg--dglllvIALVAVAILLAYYPFNAYPARVFNGDSGSLFIGGLLGAIAVVFHlefvvLTLLLPHILNGFFVVVSFr 269 Thermofilum pen...
YP_001540799 175 imtn--nitllqMTLTLLIPSIILFAFNKYPARLFNGNGGTYSIGALVAGLMLYSGlgtliLLVTVPYVINGLLIVVSSr 252 Caldivirga maqu...
Feature 1                                                                              
YP_843372    263 gkea-------fanlGDDGSLSQSGIyhlthlai------allrrlkGRVYERDVVLFMWGVEAVVAMLS 319 Methanosaeta thermophila PT
P96000       260 kgvs-------fgkvDSSGRLYWDEKphslth----------ivmkmGRFKEYQVVIILWGMEAIFAVIA 312 Sulfolobus solfataricus
EDY35579     233 idkvgd--ieefgvpDKNNHLKMPNKkiygvthla----iliqkkirGFATERGVVITLLSIQTLISLIV 296 Aciduliprofundum boonei T469
EDY35516     257 htsv-------fgivDENGIIRNKDGiksvlh----------wliswGNMTEKKITLAMLGIESILCIVA 309 Aciduliprofundum boonei T469
NP_634120    264 kkyph----vkfgkvREDGTLEVPNPltlkw-----------vlpyyYRVTEKQATYAMFLLTSVFCIMG 318 Methanosarcina mazei Go1
ABZ08607     257 rivehre-ikkptlhADDFKLKSTDEnhapitl-------vrllvakKPLSEKQICNEILKLTMFSGVLA 318 uncultured marine crenarc...
NP_560773    247 gikgre--kiqrptvLKNGAVYQECNspilslv--------rlavadKPLTEYEIFKTLVILTAITSALT 306 Pyrobaculum aerophilum st...
YP_876637    256 rivehrqikarpvetTDDFHLRATREkgapvtl-------vrlilarGPMKEAEVARSIFLLAAFSGILA 318 Cenarchaeum symbiosum A
YP_919824    270 glrehre-vkarpirVENGILKASDDpkapltlt------rlilsvgGPMTEKEVAKVYIAVEVVAAVLA 332 Thermofilum pendens Hrk 5
YP_001540799 253 giksre--klkpstyMNDFIIYPNLDkgstltlmkmivtdepgderkVIRGFYMVFNVATALTILLFIVL 320 Caldivirga maquilingensis...

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