2J66


Conserved Protein Domain Family
PLPDE_III_Btrk_like

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cd06839: PLPDE_III_Btrk_like 
Click on image for an interactive view with Cn3D
Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme Btrk Decarboxylase
This subfamily is composed of Bacillus circulans BtrK decarboxylase and similar proteins. These proteins are fold type III PLP-dependent enzymes that contain an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain, similar to bacterial alanine racemases, eukaryotic ornithine decarboxylases and diaminopimelate decarboxylases. BtrK is presumed to function as a PLP-dependent decarboxylase involved in the biosynthesis of the aminoglycoside antibiotic butirosin. Homodimer formation and the presence of the PLP cofactor may be required for catalytic activity.
Statistics
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PSSM-Id: 143506
View PSSM: cd06839
Aligned: 22 rows
Threshold Bit Score: 464.76
Threshold Setting Gi: 83594465
Created: 16-Jun-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of PLP and substrate bound to ornithine and diaminopimelate decarboxylases.
  • Comment:The active site is composed of residues from both subunits of the dimer. There are two active sites in the functional dimer.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                            # #                     #                   
2J66_A        11 LTKRFETPFYLYDGDFIEAHYRQLRSRTNpaIQFYLSLKANNNIHLAKLFRQWG---LGVEVASAGELALARHAGfSAEN 87  Bacillus circulans
YP_001157536  10 RPDAPQTPAYFYDLDELTRSNAALRAALPrpTELFYSLKANPHPTVVAELTAAG---CRAEVCSPGELRVALAAGcDPAQ 86  Salinispora tro...
YP_001537868  18 LRSGVQTPAYVYDLAEVRANAVRLRAALPerSEIYYSLKANPHPAILDTLREQG---ARAEVCSVGELDESLAAGwPPDT 94  Salinispora are...
BAD08372       7 LADQYGTPLYVYDLDRVAEARHDLRTSLPdeVEIYYALKANPHPQVAGALRSGEgreCRAEISSVGELAAALTAGfRASE 86  Streptomyces ha...
ZP_01574135    9 IVNKYGTPVYAYDTEKIMSQYALLKNSLPqeIGIFYSMKANPLLGICQLFKKLG---SGIETASSGELHTALCAGfPPEK 85  Clostridium cel...
ZP_01574529    9 IAHKYKTPLYLFDGDRLEETYFNMKKVLPdaFEFFYSVKANPSYGICRILQKCS---SGIEVASAGELHLALEAGfDNKK 85  Clostridium cel...
YP_384973     20 LAARYGTPFYLYDLDKVKEKCAAVRKAFGdsLELLYAVKANPNRELLAAMRGEV---DGLDIASAGELERALETGyDAGC 96  Geobacter metal...
YP_001535406   7 LAEEYGTPLFVYEADTVAAQYTGLRQRLHpaMDIFYSLKANPNLALCGHLRSLG---AGAEVSSLLELTTAREAGvPPGD 83  Salinispora are...
EDQ09957      16 IATTAGTPFYLYDASVLRGRLDALRAALPq-VDFFYSLKSNPNLSVTRVLHGHG---AGCEVSSLLELETSLQAGaTPER 91  Phaeobacter gal...
ZP_00631668   15 AAARFGTPAYVYATDVVEERLAHLRAHLGrwFAVSFAVKSNPNPALLGWMRDRL---DYLDISSGGELALGREAGwDAAR 91  Paracoccus deni...
Feature 1           #                                              #                             
2J66_A        88 IIFSGPGKKrSELEIAVQSgiYCIIAESvEELFYIEELaekeNKTARVAIRINPDKs--fGSTAIKMGGVPRQFGMDESM 165 Bacillus circulans
YP_001157536  87 VLYTGPGKRdIDLAEAVRAgvELFSVDSpYGLDQLDRMarehDVEVRALVRINDDTp--aPGQGLTMTGVASQFGADVRW 164 Salinispora tro...
YP_001537868  95 LLYTGPGKRdQDLRHALRRgvREFSVDSpIGLDQLDRLageaGVQARCLLRINDDQp--vPGQGLTMTGVASQFGVDVSW 172 Salinispora are...
BAD08372      87 ILYTGPGKTdGELDEAIGKgvKTFCVESlTDLQHVGAValrhGVVADCLLRINSATa--sATTSIRMTGTPSQFGIDSET 164 Streptomyces ha...
ZP_01574135   86 IIFTSPGKTyDELKYAVETgiYSINIESlEEAEVINEIadkkGRVVDISIRINPDFd--iAGSGIKMSGVASQFGIDQIR 163 Clostridium cel...
ZP_01574529   86 ILFTGPGKTySELEFAVDSkiLAINAESyKEIMLIDDIakakNKIVDVGLRIHPNFkiesKNPAISMMGTGTQFGVDIDE 165 Clostridium cel...
YP_384973     97 ISFAGPGKTrDELRRSLEAgvGCISVESlRELNDLRDLvragSHRAYILVRVNPQLl--iRDFAVKMGGKASQFGIDEEE 174 Geobacter metal...
YP_001535406  84 IVFPGPGKSrEEITACLDAqiYAIICESfEELELVNDLarerGVRARVALRVNPAFa--vKGSGLTMGGKPRQFGIDEAH 161 Salinispora are...
EDQ09957      92 ILMVGPGKSeTELARAIELgiKAIVVESaHELTQIDALarqqGRVQNIALRVNPDFh--aGGAKLNMSGRPTQFGIDQSE 169 Phaeobacter gal...
ZP_00631668   92 ISFTGPAKReAELRAAIEAglGELVLESlREARLADAVaaemGRVQPVLVRLAPARv--pKGFGDQMAGRPSPFGVDVED 169 Paracoccus deni...
Feature 1                              #  #                                       ##             
2J66_A       166 LDAVMDAVRsl-qFTKFIGIHVYTGTQnLNTDSIIESMKYTVDLGRNIYEry---giVCECINLGGGFGVPYFsHEKALD 241 Bacillus circulans
YP_001157536 165 VLDQPDLFGpr-rNVCPVGFHLYMGSNvDGEDALIAQFTQSVDTIRRLVAat---grQPELIDLGGGFGAPFArAGARPD 240 Salinispora tro...
YP_001537868 173 VFADPARFTgg-lNAPVVGLHLYMGTNmTEVHDLLAQFGRSLATAAQLRCalarhgvRLRVVDLGGGFGAPFAcVGEPVV 251 Salinispora are...
BAD08372     165 LVDAMPELRav-pGTRITGLHFFPLSNaRDEASLIGEFRHTIAYAAGLAEet---glTLEFLDIGGGFAHPYGaPGERPV 240 Streptomyces ha...
ZP_01574135  164 LDSVLNELKal-qNLNIMGIHIYTGTQaLNAHSIVQSMEQIVKLALDTSEky---vfKLKFLNLGGGFGVPYFkDDSTLD 239 Clostridium cel...
ZP_01574529  166 IPEILKYIKss-kNLNLVCFHVYAGSQiFQPDTTLAYFEQTINIFKELIEkn---dlHIKVLDFGGGFGVSYDgKNKPYD 241 Clostridium cel...
YP_384973    175 LPSALDFIKanadAFDFKGIHIYAGTQcLNEEALAQNLANTLAIAARITTdy---gmECRVINIGGGLGVSYYeEHPGLD 251 Geobacter metal...
YP_001535406 162 LFALPGLRLa---HLRVVGVHVYLGTRiLDETVIAENTRRILELAERLAAhl---dfPLEFVDVGGGLGVAYFdGERDLA 235 Salinispora are...
EDQ09957     170 LPDVLKQAEsc-aHLRLCGLHAYMGTRiLTHETVVANVGNILNLATEVIStl---kaPLDFVDVGGGFGIPYYdGETELD 245 Phaeobacter gal...
ZP_00631668  170 AAEILPQIAal-pNLRIIGLHIYSGTQcLKPEAICENWRIFMQVFRDTCAal---dlRPEKLIFGAGLGIPYHpGDSALD 245 Paracoccus deni...
Feature 1                                    ####                                                
2J66_A       242 IGKITRTVSDYVqea-rdtRFPQTTFIIESGRYLLAQAAVYVTEVLYRKASKGEVFVIVDGGMHHHAASTFrg-rsMRSN 319 Bacillus circulans
YP_001157536 241 LPKLADRLAALLadafpgwPEQGPAVAFESGRYLVGTCGTLVTRVLEVKRSQGIPVVVLESGIHHLGGMSG-----LRRL 315 Salinispora tro...
YP_001537868 252 LSGLRDGLYRLLdaelpgwRDGAVRVVFESGRYLVGTAGTLVTRVLDVKRSHGRDLVVLESGINHLGGMSG-----LRRL 326 Salinispora are...
BAD08372     241 YRELRTELAAALdehfphwREGAPRIAVETGRYQTSGAGTLLTRVVNIKVSRGRKFVVIDAGINTFGGMSG-----LGRL 315 Streptomyces ha...
ZP_01574135  240 MDILRNEFSEMWnky--kdRLFNTQVFVESGRFLMAESGTYLTKILYRKECKGRKYLVCDGGSNQHAASAFlg-rhIRNN 316 Clostridium cel...
ZP_01574529  242 FEYFGAEINKLYhky--edFFQGKKLVFESGRLLLAESGVFLTEVQYRKVLHDKVFLITDGGMNHNSLSTFre-kkIRGN 318 Clostridium cel...
YP_384973    252 LDKLALLFRAEFdry-reaTGTKPRILLELGRFLVAEAGIYVTRVVSEKLSRGEQFYVLDGGMNHHLSASGnlgmtIRKN 330 Geobacter metal...
YP_001535406 236 VERLTDLVNPVVadf--hdRHRDTRLIMELGRYLVAPAGTYLIRVRYVKSSMGERFAVTDGGTHQHMAAVGig-sfVKRD 312 Salinispora are...
EDQ09957     246 LDALGQAVTPLVqsf--gaTHPKTRVVIELGRYLSGPSGQFVTRVQQTKSSKGEHFAVCDGGSNVHVAAAGqg--fLRKN 321 Phaeobacter gal...
ZP_00631668  246 LAAIAAEIGPELdafraepRLADARLVLELGRYLVGPAGWFVTRVLSVKQSRGTRIAICDGGLNDNLAASGnfgmvLRRN 325 Paracoccus deni...
Feature 1                                    ##                           #   #    #           
2J66_A       320 YPMEYIpvreds--grrelEKVTIAGPLCTPEDCLGKDVHVPaLYPGDLVCVLNSGAYGLSFSPvHFLGHPTPIEILK 395 Bacillus circulans
YP_001157536 316 PPIVPDlltvda-aagppiPGTIVTGPLCTPLDTWARATALPpLVPGQLVAVPNVGAYGLYASLvAFLAHPMPIEVTI 392 Salinispora tropi...
YP_001537868 327 PPLNPHllsdt---aagdlRPTMVAGPLCTPLDLWSRNAPLSpLHPGDTLQVPNVGAYGLSASLlAFLSHPTPTEVAV 401 Salinispora areni...
BAD08372     316 LPVAVEpeytgsaeateltDVASLAGPLCTPGDILGREIRLPeLAPGDLVTIPNAGAYGVTASLlMFLGRPAPVEVVL 393 Streptomyces hals...
ZP_01574135  317 FPIQILnks-------sneEQVNVVGPLCTPTDVIGQNVMLPeAVPGDIVAVLKSGAYGVTQSPgLFLSHPLPAEVQY 387 Clostridium cellu...
ZP_01574529  319 FLMKILdnr-------neeETVTVAGPLCTPEDILGRNVTLNkADRGDILCIKNTGAYGSSFSPlGFLGHPAPCEVLI 389 Clostridium cellu...
YP_384973    331 YLVRNLsrpg------aerIICTLVGPLCTPLDLMGKGVTVEvPEVGDLVGFLNSGSYGYSASPlLFLGHGEPCELLV 402 Geobacter metalli...
YP_001535406 313 FPLRLLgrpt-----dgplVRWTVTGPLCTPNDTLVRAAELPdLRRGDLLGVLRSGAYGPSASPtLFLGHGHPAEVLV 385 Salinispora areni...
EDQ09957     322 FPIRLLrdgkae-iddeaaQPWTLTGPLCTPQDVIGKSVLMAtPQVGDLISIGQSGAYGPTASPvNFLGFGAPAEVMI 398 Phaeobacter galla...
ZP_00631668  326 YVMHRVggded---gtrpeEKQDISGPLCTSIDKLGGGVALPrLEEGDLIAIHACGAYGPTASPlHFISHPMPAEVLL 400 Paracoccus denitr...

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