Conserved Protein Domain Family
PLPDE_III_D-TA

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cd06821: PLPDE_III_D-TA 
Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme D-Threonine Aldolase
D-threonine aldolase (D-TA, EC 4.3.1.18) reversibly catalyzes the aldol cleavage of D-threonine into glycine and acetaldehyde, and the synthesis of D-threonine from glycine and acetaldehyde. Its activity is present in several genera of bacteria but not in fungi. It requires PLP and a divalent cation such as Co2+, Ni2+, Mn2+, or Mg2+ as cofactors for catalytic activity and thermal stability. Members of this subfamily show similarity to bacterial alanine racemase (AR), a fold type III PLP-dependent enzyme which contains an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain. AR exists as homodimers with active sites that lie at the interface between the TIM barrel domain of one subunit and the beta-sandwich domain of the other subunit. Based on its similarity to AR, it is possible that low specificity D-TAs also form dimers in solution. Experimental data show that the monomeric form of low specificity D-TAs exhibit full catalytic activity.
Statistics
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PSSM-Id: 143495
View PSSM: cd06821
Aligned: 15 rows
Threshold Bit Score: 411.302
Threshold Setting Gi: 149923112
Created: 18-Jun-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of the PLP cofactor and substrate analogs to other members of the superfamily.
  • Comment:The active site is composed of residues from both subunits of the dimer. There are two active sites in the functional dimer.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                              # #                   #                  
ZP_02925423  10 VENIDEIPSPSLLVYPDRIEENLRRMIAMVG-DVQRLRPHMKTHKMPDVIKLHLAlGITKFKCATIAEAEMTAEAGAk-- 86  Verrucomicrobium...
ZP_02736583   7 LSDQATIFSPALVFFPALIKQNINRVVEMAG-GPARLRPHVKTHKTVEIVKLLLEaGVTKHKCATIAEAEMLAHAGAp-- 83  Gemmata obscurig...
ZP_01852393   7 IDDTSQIISPGMIIFKDLLEDNLRKMIELIG-DPSRLRPHCKTHKMREIIQLELSlGILKHKAATFAEAEMLADTGVk-- 83  Planctomyces mar...
ZP_01911527  22 APLEDELITPALVIDLDAVEHNIAAMVQRAG-SPQRWRPHLKTTKQAVVISALIRaGLRHLKVATLDELELVLRTGAaed 100 Plesiocystis pac...
ZP_01107367   9 LKDTAEVISPSLMVYPDRIEKNIQLMVKMAG-GTEFLRPHIKTHKMAEIVNMQMQhGIRKFKCATIAEAELLAQCGAe-- 85  Flavobacteriales...
ZP_01120393  16 IQNAREVPSPALLVYPERIRRNIDAMIRIAG-SPDRLRPHIKTHKTAEIIQMQRGrGIDRFKCATIAEAELLGSLGAg-- 92  Robiginitalea bi...
ZP_02082754   8 LKHTDEIITPCLIYYKDIIEQNIKEMISVAG-NANRLWPHVKSHKMKKLVELQIKmGITRFKCATIAEAQMVAESGAs-- 84  Clostridium bolt...
ZP_02083121   9 LKNTDTIISPSLIYYEEIIRENIKKAIRTAG-SPERLWPHVKSHKSKDMVRMQMEyGITKFKTATVAEAEMAAEAGAe-- 85  Clostridium bolt...
NP_867288    24 VSNVDQVYSPGLLVYADRVQANLDRMIEMAG-GADKLRPHVKTHKMPAIVELSVAsGITRFKSATIAEAEMTAAAGAv-- 100 Rhodopirellula b...
YP_591845    18 IDSIESAITPALAIYLDLVDANIRTTLRLLNgDPDRWRPHVKTAKLAAVMSRLVEsGVTNFKCATTLELLTACEVGAr-- 95  Acidobacteria ba...
Feature 1              #                                                         #      #       
ZP_02925423  87 ---DILLAYPLVGPNIARFLKLkRQYPQTRFCAVADD------ADAARALSaaagkeGVTVEVMLEVNCGMARTGIApge 157 Verrucomicrobium...
ZP_02736583  84 ---DVLIAYPLVGPNIQRACELiRKYPGTTFSALIDD------ATATRALSatmaaaGRTIGVVLDLDVGQHRTGIAvgp 154 Gemmata obscurig...
ZP_01852393  84 ---DICLAYNLVGPNIARAVEFrKRWPDVSLQVTADH------PGPIELLGeamsaaGLEIEVLLDLNTGQNRTGLPpge 154 Planctomyces mar...
ZP_01911527 101 valDVLVAYPLQRAACRGALALaARWPEAQIHVLADS------PAHLEALAaw--sgSDPLSVLLDVDLGMGRTGSAp-- 170 Plesiocystis pac...
ZP_01107367  86 ---DILLAMQPVGANMTRYFELmDKYSSSDFSTLVDNq-----KTANEFIEhsk-krGMKTSLWLDINNGMDRTGIKvgt 156 Flavobacteriales...
ZP_01120393  93 ---DILLAYQPVGPDQERLLRLqAAYPGVRFAALVDH------EETLKALGelatshNAQIRLYLDLNTGMDRTGIVpga 163 Robiginitalea bi...
ZP_02082754  85 ---DALVAYPLVGPNIDRFLELvKTYAGTRFWAIGDD------LEQVSLLNsaagsqETIVNFLADVNMGTNRTGISin- 154 Clostridium bolt...
ZP_02083121  86 ---KVILAYPLIGPNMERFVKLaKAYPDTVFYGVEDD------LAQFEALSgvcvehNAVLPMLVDVNMGMNRTGVPie- 155 Clostridium bolt...
NP_867288   101 ---DVLLAVQPVGPNIGRFIKLiQRYPGCLFSCLVDDlsvlasLEAAFELA------GLQGSVWIDIDVGMHRTGIApnd 171 Rhodopirellula b...
YP_591845    96 ---DVLVAYSNHGARAVRIKEIaAMFPKVRISVVVEA------LDEVSTWR------HSGISAFIDVDPGMNRTGISqd- 159 Acidobacteria ba...
Feature 1                               #                                                 ##    
ZP_02925423 158 gAIQLYQLLAt--lPNVQVAGIHAYDGHIHdhdlaartsgVEVAYQPVEALRDRLLalg----lsVPHYVVSGTPTFGIH 231 Verrucomicrobium...
ZP_02736583 155 gALALYELAGq--lPGLRLDGFQLYDGHNSqpdraereqaVRAFIKPVLDLRAAAEakg----lpVPRLVCGGTPSFPVY 228 Gemmata obscurig...
ZP_01852393 155 nAVELYQMIAn--tPGLIAAGLHAYDGQNHqvdinereiaVKEVWNNVSRLRDQLVteg----lpVPRIVAGATGSFPIF 228 Planctomyces mar...
ZP_01911527 171 dRWRALEATP----EGLSLAGLHGYDGHHRwth----reaAHAGYGQLCALAQAFLerpalriqgAFELLTSGTHSYAHA 242 Plesiocystis pac...
ZP_01107367 157 eALSIYKVINn--sPFVVSKGLHTYDGHIRneqiterenvCNTTFKPVLEFKKLLEeks----lgPIEMVAGGSPSFPIH 230 Flavobacteriales...
ZP_01120393 164 aAAALYRKIAa--hPHLEACGLHAYDGHNRqtdtserqaaADKAFEGVAKLRDQLVseg----ldVPAIIAGGSPSFPMH 237 Robiginitalea bi...
ZP_02082754 155 tLPDFYRNCCk--lPWLSVQGLHCYDGNNGiaeydarekaVDKIDRAVFSTVAALEqtg----lpCPVLVMGGTPSFPCH 228 Clostridium bolt...
ZP_02083121 156 rLEELYRSASs--lPGLRLCGMHCYDGNHNnkdaavrqaqVDDTDKKVSDIMERLNgdg----icCELAVAGGTPSFPCH 229 Clostridium bolt...
NP_867288   172 sATELVRSIQt--sDHVRLAGIHVYDGHLHdtdseklrrqFDEAVKPFWIWFEDIQras----ggQLNLVAGGTPTSAFW 245 Rhodopirellula b...
YP_591845   160 cEEDIVRLAQglqtEGVPFRGLHYYDGHHRnanlsereqaAHHGYDHLLRIVGALQqhg----lpVEEVITAGTPAFPAS 235 Acidobacteria ba...
Feature 1                             ####                                          #           
ZP_02925423 232 ARrg--------------sYECSPGTCVVWDWGYGTKHPDL-DFLHAALVVTRVISKPApeRLTLDLGHKAIAAENPHPR 296 Verrucomicrobium...
ZP_02736583 229 AAmtd-----------vpgIECSPGTFVLHDAGYGPKYADLaGITPAAVLVTRVISRPTpnRVTLDLGNKAVAADPLLEK 297 Gemmata obscurig...
ZP_01852393 229 AGidd------------pdIEVCPGTCVLHDVGYGELFPDL-KFTPAALVLTRVISRPDaeRITFDLGYKAIASDPAMEN 295 Planctomyces mar...
ZP_01911527 243 LAypgfaeskeapspnpwlHRVSPGTIVFNDRRSKPAAEDL-GLRQAAFVASRVISVGPg-RATLDAGSKGVTPDADSRG 320 Plesiocystis pac...
ZP_01107367 231 SKrk--------------dVECSPGTTLLWDERYGSSFAES-KFLTSAVLITRLISKPKegLLCFDLGHKSIAPEMEFPR 295 Flavobacteriales...
ZP_01120393 238 ASre--------------gVELSPGTTLLWDARYQSLFPDM-PFEIAAVLLTRVVSHPRpgIACLDLGHKAIAPEMDFPR 302 Robiginitalea bi...
ZP_02082754 229 ARyk--------------rVYLSPGTGIISDFGYASKFTDE-TFIPAGLLMTRVISHGGpgLFTLDLGYKGIAADPPGLK 293 Clostridium bolt...
ZP_02083121 230 AGat--------------rWYLSPGTAFITDAGYYMNLPDL-DFIPGAAVMTRVISHPAkgVFTTDLGYKAIASDPAGQR 294 Clostridium bolt...
NP_867288   246 QAnaqa---------rqvkVETGAGTTVLWDAGQPTFSPSM-PFEAAAAVLTRVISRPTefDVCLDLGHKAIASEMPLPR 315 Rhodopirellula b...
YP_591845   236 LTysgfq-------raefkHRMSPGTVVYGDMSSLAQLPQEwGYQPAALVVSTVVSHPQadVFTCDAGHKSVSADAGIPN 308 Acidobacteria ba...
Feature 1                                                                                      
ZP_02925423 297 VY-LLNLPDAKAVMHSEEHLAVETPla---gdFPVGSVVYGVPWHICPTVALHSHAFVVRGGRADgTWKVAGr-ERVIT 370 Verrucomicrobium ...
ZP_02736583 298 RVtLLDFPEYKTVGHNEEHLIVETAga---daYKPGDVVYALPGHICPTVALHHHVLVAEGGKVVgKWDVASr-VRTLT 372 Gemmata obscurigl...
ZP_01852393 296 RCrFPDLPDAKPVLQNEEHLVVLSEra---adFQPGDELLAIPRHVCPTSALHKSVTVVSDGKVVdHWNVAAr-DRYIT 370 Planctomyces mari...
ZP_01911527 321 SCaVLGHPALSPRRASEEHLPLALPepslessLEFGALAWLVPDHVCTTVNLHREALWIRGDRLVgRGPIVSghRPRAP 399 Plesiocystis paci...
ZP_01107367 296 MK-IFGLENCEQIGHSEEHLVVKCEna---daHEVGDVFYAIPMHICPTVAKYEEVFTVLSNEIAgSWKVAAr-NQKIN 369 Flavobacteriales ...
ZP_01120393 303 VA-LPELPGCRQVGQSEEHLVLDCGeg---avPEIGQVLYGFPMHICPTVAKYPELLVVEDRQVRkAWKVAAr-DYKIK 376 Robiginitalea bif...
ZP_02082754 294 GC-LMGDWHASPINQNEEHWIWKMDegyedqrPGIGSVLYVIPTHICPTSALYPEALVALDGQIVdIWEVTAr-NRKLS 370 Clostridium bolte...
ZP_02083121 295 GY-IVGLEDAVPIIHSEEHWAFRLEdes--riPAIGSCLYVIPTHICPTTALYPEILVAKEGQVAdRWQVTAr-NRRIT 369 Clostridium bolte...
NP_867288   316 VSwLNFDADHEVVGHNEEHMVLRTKqp---cqLRCGDVLYGVPVHICPTVALHQHAECVRDHRAIeKWPVVAr-NRMLS 390 Rhodopirellula ba...
YP_591845   309 CS-VVGHPEFEPLHPSEEHLPVRVPsgs--arPSRGNILYLVPRHVCPTVNNFDHALIIRDHRIVeVAPVTAr-GREVP 383 Acidobacteria bac...

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