Conserved Protein Domain Family
PLPDE_III_LS_D-TA

?
cd06819: PLPDE_III_LS_D-TA 
Type III Pyridoxal 5-phosphate (PLP)-Dependent Enzyme Low Specificity D-Threonine Aldolase
Low specificity D-threonine aldolase (Low specificity D-TA, EC 4.3.1.18), encoded by dtaAS gene from Arthrobacter sp. strain DK-38, is the prototype of this subfamily. Low specificity D-TAs are fold type III PLP-dependent enzymes that catalyze the interconversion between D-threonine/D-allo-threonine and glycine plus acetaldehyde. Both PLP and divalent cations (eg. Mn2+) are required for catalytic activity. Members of this subfamily show similarity to bacterial alanine racemase (AR), which contains an N-terminal PLP-binding TIM-barrel domain and a C-terminal beta-sandwich domain. AR exists as homodimers with active sites that lie at the interface between the TIM barrel domain of one subunit and the beta-sandwich domain of the other subunit. Based on its similarity to AR, it is possible that low specificity D-TAs also form dimers in solution. Experimental data show that the monomeric form of low specificity D-TAs exhibit full catalytic activity.
Statistics
?
PSSM-Id: 143493
View PSSM: cd06819
Aligned: 27 rows
Threshold Bit Score: 373.475
Threshold Setting Gi: 197104470
Created: 18-Jun-2008
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of the PLP cofactor and substrate analogs to other members of the superfamily.
  • Comment:The active site is composed of residues from both subunits of the dimer. There are two active sites in the functional dimer.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                             # #                   #                    
YP_551594     12 SLDAVDTPALVLDLDAFERNLHRMADALkgsQVRLRPHAKSHKCPEIALRQIALGAVGVCCQKVSEAAVFVAAGVRDVLI 91  Polaromonas sp....
YP_127774      9 HKIELNTPCLVIDYEKLKYNLKMMQQHGfkwGIGIRPHCKTHKCTQIARLQLEYGAIGISAAKLSEAEVLVSKQITNVLI 88  Legionella pneu...
YP_001411894  18 SRAAIPTPALVLDLDAFERNVARMAEHCkvsNLSIRPHAKTHKCVTIAKAQIAAGAVGQCCAKLGEAEAMAAGGIESILI 97  Parvibaculum la...
EDT53328      22 GRKRLNTPSLILDLDVFDANVARMQAICdanGVNLRPHAKSHKSSRIAQAQREAGAIGVCCATINEAEAMAVAGVGGLLI 101 Methylocella si...
YP_618211     17 SRADLNTPVLILDREALDRNIARMAALTkaaGVALRPHAKTHKSVDIARRQLDAGAVGVCCAKIGEAEILADGGITGILI 96  Sphingopyxis al...
ZP_01898531    5 HKLELDTPCLVIDKRKLMHNIETMQKFAaakSKHVRPHAKTHKCVEICQLQLDAGSIGISITKPSEAYELAKAHIRHLLI 84  Moritella sp. PE36
ZP_02736918   14 DVADLDTPQLLVDLDVVDGNVRRMFAAArgrGVRVRVHFKSLKCTGLARHVAAAGPDGFAAAKLNEAEALADAGVTDILL 93  Gemmata obscuri...
ABX10644       6 PKADIDTPALCIDLNQLDANIKTMADFIisrGKQWRPHEKCHKTPAIALKQIAAGAIGVTCAKVSEAEVMAAAGIHDILI 85  uncultured plan...
EAT84851     348 SLDSLDTPSMIVDLDLMEANIKKLMERLlptGVNIRPHLKTTKSALLAQRLVDAGAKGGCVAKLSEAEVMCAKGFDDLLI 427 Phaeosphaeria n...
ACB85504      10 KLENLPTPALLLDMSNLEYNINLLADFFrdkPCDIRPHMKGHKNLTIAHMQIDRGAIGVTCAKTSEAEVMVNGGIKDILI 89  Natranaerobius ...
Feature 1        #                                                     #      #                  
YP_551594     92 TNEVVGpVKIRHLMELARTAh-LGVLVDHa-SQVPALAAAAqa--hSVTLDVYVEVDVGAHRCGVApGAEAAQLARLVVA 167 Polaromonas sp....
YP_127774     89 TSPIVSeAKYSRLFNSLKSApdLMLVVDGk-DNIRMLNELGks--lNQPVYVLLDIDSGIGRTGIN-PEAALEYGHFING 164 Legionella pneu...
YP_001411894  98 TSPVVTvQGIERLMALNEKMpdLMVVVDNp-VNARALAAAAae--kKRVLKVLVDLDVGLHRTGIRpGEDALALAELLDA 174 Parvibaculum la...
EDT53328     102 TAPLVTdVKISRLCDLCARApdTMAVIDNa-DNALALSAAFa---gRPALNVLVDLDIGHHRTGAVdPAAAFKLARMIAA 177 Methylocella si...
YP_618211     97 TSPVAApAAIARLAKLAARAegLMAVVDHpaVAVRVDAALAv---eGARLDVIIDIDPGIARTGVAsAEAAVALAQTIDA 173 Sphingopyxis al...
ZP_01898531   85 TSPIVTkHKLATLAKIIKLApeTMIVVDSe-TNLTQLNQLGse--lNQRINLVVDIDAGIGRTGAS-FETAFDLALSVHR 160 Moritella sp. PE36
ZP_02736918   94 ANQVVGlHKLRRAARLARRAd-LIVCVDDa-DNARALSAAAva--eGTQIRALVEVDIGMARCGVPpGAAAVELAQTVAA 169 Gemmata obscuri...
ABX10644      86 ANMIVGtPKWERVAALCRTTd-VIIACDHf-AQVEPLAAICrr--nGVTCRVIVEVDIGLNRVGTRpGVDTTELARSIHA 161 uncultured plan...
EAT84851     428 TCEIIGaPKVKRLAELFKKHksIRIVVDSe-VGARAINDELkksgvDGRIAVLIDLDVGLHRTGIQpGEPALKLAACIAQ 506 Phaeosphaeria n...
ACB85504      90 ANQVIEsGKINLLAELARRAd-IKVAVDSy-QNCENLSRIAve--hGVTIGVLVEMDIGLGRCGVKsFDEGINLVQEIQK 165 Natranaerobius ...
Feature 1                   #                                             ##               ####  
YP_551594    168 sPPLRFAGLQCYHGPAQHLRTpRERAQAIGDAVRRVRQTLAaie-acGIAVKCVTGAGTGTFlHERDSGVYNELQAGSYV 246 Polaromonas sp....
YP_127774    165 mNWLKLKGIQCYAGHLQHIQNyEERKAFSLRIMEMASRIVKsfr-etNLPCEILTGTGTGTFdIDVEASEVTEIQPGSYA 243 Legionella pneu...
YP_001411894 175 aEFLELRGLQAYAGHLMHIHDfAERREKSLAAMKVLGDQRDalk-akGLPCDIVSGGGTGSYnIDPEAKVLTELQGGSYV 253 Parvibaculum la...
EDT53328     178 tPNLTLQGVQAYGGHFQHIPDaAKRGAAAAQGREQLKALLAlmr-daQLETGIVTGAGTGTHgLDGSSGVFTEIQAGSYI 256 Methylocella si...
YP_618211    174 lPGLAWRGVQYYCGSQQHIESyAERRAAIIERTDYLQGVIAala-daGFAPPIVTGSGTGTHrIDLDLGVFTELQAGSYV 252 Sphingopyxis al...
ZP_01898531  161 nANTTLKGIQCYAGHFQHILDnEERKQASATLLNKAGKLKQdiesatGLVNLIQTGSGTGTYeIDSNIASVTEIQPGSYT 240 Moritella sp. PE36
ZP_02736918  170 lPGLRFYGLQGYDGHLQMLPDyESRQAQSLAGAEALADTRRqve-raGLPVALVTGGGTGTWeFAAAVPGVTEVQPGSFV 248 Gemmata obscuri...
ABX10644     162 lDGLQLAGIMGYEGHLLTIADrDEKRTKIAEAMNVLVHCRDell-gaDLPCEIVSAGGTGSFqITSDCEGITELQAGGGI 240 uncultured plan...
EAT84851     507 lDHMALIGVQGYEGHLQHVHSfEERKAKCLESMQILTSTANalr-eaGHQIDVVTTGGTGTAeFCISVPGVNEVQPGSFI 585 Phaeosphaeria n...
ACB85504     166 lDNLEFRGVMGYEGPFRDKTQsEKSDMVKESLSRVVQLKRRie--qeNIQVEIVSCGSTGTWdITGNYPEITEIQAGTYP 243 Natranaerobius ...
Feature 1                                                     #                                  
YP_551594    247 FMDRDYGDnqrg---egdvAFEHALFIKTTVMSRPTAeRAVVDSGLKASSIDSGLPVv--WQRPELRYAk--AADEHGVI 319 Polaromonas sp....
YP_127774    244 VMDVEYGSigskntpssfqLFKNSMTLLTSVISSNRReHATVDAGTKSIYVDPHHKPh-iISHPRLVYDwsgFGDEHGKV 322 Legionella pneu...
YP_001411894 254 FMDKQYHDvalgn--gasfPFETSLFVQMSVVSANTKnLATTDAGFKSFSTDAESPKl-lSGAPEGAGYf-fFGDEQGGV 329 Parvibaculum la...
EDT53328     257 FMDAEYATinye---ggdwPFGFSLFVQTSVLSANLPtQVTTDAGTKSFALNGPPPRivsASLHGAAYGf--AGDEHGRV 331 Methylocella si...
YP_618211    253 FMDKQYLDcelae--geaaPFEVALAVDARVVSANHSgLVTIDAGFKSLSTDGGVAAv-rRGAPETAFFa-fMGDEHAAL 328 Sphingopyxis al...
ZP_01898531  241 VMDKEYFDiey-----sagHFQPAMTLLTSVISANHAsHVTVDAGTKALYKEATHPQi--ISHDSLSYEwhyFGDEHGKV 313 Moritella sp. PE36
ZP_02736918  249 IMDAAYHKvr--------pEFGCALSVRAAVVSRRPG-QYVLDAGSKAISRDFGTPEi--KGRPGDRVLr--LNEEHTIV 315 Gemmata obscuri...
ABX10644     241 FADPHYQThcd------lqDLPSSLTVLATVVSRPSLeRAILDAGRKSHHPDFHMPIv--KGHSDAEVVr--VSAEHCEV 310 uncultured plan...
EAT84851     586 FMDTDYRAai-------gtTYSNSLTILSTVLSTQGPrIITIDAGLKSLTTDSGLAE---CKTPGYKYGv--LGDEHGSL 653 Phaeosphaeria n...
ACB85504     244 LMEVPYLEdg--------lPFKSALSVLATVISKSHD-KLILDCGLKAVTDDQGIPKl--MDYDSPIVG---INEEHSIV 309 Natranaerobius ...
Feature 1                                                            
YP_551594    320 ltpd---aaaLSLGDALMLVPGHCDPTVNLYDELVCVRGQRVEALWPVAARG 368 Polaromonas sp. JS666
YP_127774    323 fscdg-gehfLQNGDVLELIVPHCDPTINLYDKFYITENNVVIDIWDIDMRG 373 Legionella pneumophila str. Lens
YP_001411894 330 llpd---gasLPIGSVLTAMTPHCDPTVNLYDAYHVVRGDTLVDIWPIEARG 378 Parvibaculum lavamentivorans DS-1
EDT53328     332 mleh--gmgrPQIGERLECIVSHCDPTVCLYDYFVCVRGQKVVDVWKIDARG 381 Methylocella silvestris BL2
YP_618211    329 iapg--igdmLHPGDPVSLTVPHCDPTVNLYDHYHVVDGDTLIDIWPVSARG 378 Sphingopyxis alaskensis RB2256
ZP_01898531  314 tge-----slPEIGDVIEMIVPHCDPTINLHDKFYVIENDIVVDVWDIALRG 360 Moritella sp. PE36
ZP_02736918  316 aad----gavPAVGEAAEVLPAHCCATMNLHRACAAVRGGRVEAVWPIECSG 363 Gemmata obscuriglobus UQM 2246
ABX10644     311 klgp--kaqdLKIGDKIELVPGYSDFTTVMHEAFYGFRDERLESVWPIQGRG 360 uncultured planctomycete 3FN
EAT84851     654 ewedgsdarpLKVGDRVEMVPSHIDPTINLHDTYFAYRKGVIEEIWAVDSRG 705 Phaeosphaeria nodorum SN15
ACB85504     310 eidh--etekLEIGDKIRILPAHSCTTVNLYDYYCCLRNNEVVDIWQVHARG 359 Natranaerobius thermophilus JW/NM-WN-LF

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap