2Z7E,2QQ4,1XJS,1SU0


Conserved Protein Domain Family
IscU_like

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cd06664: IscU_like 
Click on image for an interactive view with Cn3D
Iron-sulfur cluster scaffold-like proteins
IscU_like and NifU_like proteins. IscU and NifU function as a scaffold for the assembly of [2Fe-2S] clusters before they are transferred to apo target proteins. They are highly conserved and play vital roles in the ISC and NIF systems of Fe-S protein maturation. NIF genes participate in nitrogen fixation in several isolated bacterial species. The NifU domain, however, is also found in bacteria that do not fix nitrogen, so it may have wider significance in the cell. Human IscU interacts with frataxin, the Friedreich ataxia gene product, and incorrectly spliced IscU has been shown to disrupt iron homeostasis in skeletal muscle and cause myopathy.
Statistics
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PSSM-Id: 143480
View PSSM: cd06664
Aligned: 206 rows
Threshold Bit Score: 46.4737
Threshold Setting Gi: 87199971
Created: 8-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetrimerization
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:This conserved cysteine cluster is the putative site for the assembly of the nascent Fe-S cluster.
  • Structure:2Z7E_A/B: a single [2Fe-2S] cluster is bound to the asymmetric trimer of Aquifex aeolicus IscU, contacts at 4 A.
    View structure with Cn3D
  • Comment:In the A. aeolicus IscU trimer, the [2Fe-2S] cluster is buried at the interface between two subunits, the third subunit further stabilizes this interaction.
  • Structure:1XJS: Bacillus subtilis IscU binds zinc, contacts at 4 A
    View structure with Cn3D
  • Structure:1SU0: IscU from Streptococcus pyogenes binds zinc, contacts at 4 A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                        #                          #                    
2Z7E_B         4 EYNEKVLDHflnprnVGVLedangVGQCGNPaCGAAMLFTIKVnpendVIEDVRFKTFGCGSAIAVSSMLTEMVKGk--- 80  Aquifex aeolicus
YP_001545297   5 EAIEFLLEHyeqprhRGELadpdvTMPGGNPgCGDVVTIYLKVdaandAIQDLAFVGEGCTISQAAASILLEQMQGqa-- 82  Herpetosiphon a...
YP_001430465   5 ALIARLLDHyehprnRGSLpdadvTQTGGIPdCGDVVTVYLKIdp-pdRVVAISFEGQGCTISQAAASILSERVIGstfa 83  Roseiflexus cas...
YP_644950      5 LQIQILLDHyerprhRGRLeeadvRMPGGNPgCGDVVTIHLKGaddhrHIEDVTYEGEGCTISMAAASMILEEVHErn-- 82  Rubrobacter xyl...
YP_002522382   4 STFELLRDHvrnprhHGPLedatiVLEGGNPeCGDVVTVYLKIapdgeTIEDVHFTGQGCGVSQAAASLLMERLHQgh-- 81  Thermomicrobium...
YP_002309473   1 MYNDIIIDNfsnpkfVGDLesadyKFEIGNPvCGDRIHMQVSFdg--dNIRESSFRAWGCATSVATANIFCDSIIGksid 78  Shewanella piez...
YP_001705396   3 RFSPAVIDHftnprnAGRLdqadvTAFIGNPvCGDQILLSAQIrd--eVVSRIGFEAYGCSASLAVASILTERLSGmpvr 80  Mycobacterium a...
NP_931835      1 MFNNIIIDNfcnpdcQGTLsaptiKLALGNPvCGDKVDIDLTLdn-qgRVNNACFRAWGCTASLAMSNQFCRHAKGk--- 76  Photorhabdus lu...
YP_997742     10 MFNEIIVKNfsdpafASELdeataSIEIGNPvCGDRIRVQLDVtd--gLIKRARYQAWGCATSLATGNVFCACIDGk--- 84  Verminephrobact...
YP_001637061   9 DAIERLLDHyrqprhYGELpd-aiTYSGGVPdCGDTLTVYLRVap-ngRLTDLQFTGQGCSVSVGTASILIEQLQGatva 86  Chloroflexus au...
Feature 1                                        #           
2Z7E_B        81 -----pIQYALNLTykdifee-lgglppQKIHCTNLGLETLHVA 118 Aquifex aeolicus
YP_001545297  83 ----laAIEALDFNwmidel--grevvqSRPRCATLALGTLKAA 120 Herpetosiphon aurantiacus ATCC 23779
YP_001430465  84 eieaitLDEMIDFLgre--------avqTRPRCATLALRTLKAA 119 Roseiflexus castenholzii DSM 13941
YP_644950     83 ----ltMDEVLRMDynemidklgrqivaSRPKCATLGLGTLKAA 122 Rubrobacter xylanophilus DSM 9941
YP_002522382  82 ----wtIGRIAAVDfslvqdlvgpeaarSRPRCASLALSVLKAA 121 Thermomicrobium roseum DSM 5159
YP_002309473  79 ---eisMRQADEISdmlg------elepSQQHCINILSELHREL 113 Shewanella piezotolerans WP3
YP_001705396  81 --dietIQAAQVIEwsgg-------lspEQQHVAALGADVAQRL 115 Mycobacterium abscessus
NP_931835     77 -----tLEELNALSpegidal-lgelepAQQHCLDMLHKLFEQL 114 Photorhabdus luminescens subsp. laumondii TTO1
YP_997742     85 -----pLSVVLDTSnddietm-lgelepSQHHCLEMLRALFQWL 122 Verminephrobacter eiseniae EF01-2
YP_001637061  87 --dllaLDETAAVQivgs------evfqARPHCATLIFRTLKAA 122 Chloroflexus aurantiacus J-10-fl

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