1KAE,1KAH,1KAE


Conserved Protein Domain Family
Histidinol_dh

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cd06572: Histidinol_dh 
Click on image for an interactive view with Cn3D
Histidinol dehydrogenase, HisD, E.C 1.1.1.23. Histidinol dehydrogenase catalyzes the last two steps in the L-histidine biosynthesis pathway, which is conserved in bacteria, archaea, fungi, and plants. These last two steps are (i) the NAD-dependent oxidation of L-histidinol to L-histidinaldehyde, and (ii) the NAD-dependent oxidation of L-histidinaldehyde to L-histidine. In most fungi and in the unicellular choanoflagellate Monosiga bevicollis, the HisD domain is fused with units that catalyze the second and third biosynthesis steps in this same pathway.
Statistics
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PSSM-Id: 119329
View PSSM: cd06572
Aligned: 193 rows
Threshold Bit Score: 327.092
Threshold Setting Gi: 18312330
Created: 8-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residues [active site]
Evidence:
  • Structure:1KAE: L-Histidinol dehydrogenase monomer from Escherichia coli; the catalytic residues line up with a water molecule and the substrate, which in turn participates in the octahedral coordination of the zinc ion.
    View structure with Cn3D
  • Comment:the catalytic His residue participates in acid-base catalysis
  • Comment:the catalytic Glu residue activates a water molecule that attacks the reactive carbon in the second step of the reaction mechanism

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1KAE_A       32 ITRTVNDILDNVKARGDEALREYSAKFDkttv--talKVSAEEIAAASErlsDELKQAXAVAVKNIETFHTAQKlp-pvD 108 Escherichia coli
YP_825109    24 AEAVVTPILEAVRKRGDRALMDYARQFDqlqr--ksvRVPEGELLAARDgltPEFRGAIETAAPNIRAYAGRQMpr--eW 99  Solibacter usita...
YP_643880    30 VRAAVRQVVEEVRERGDEALLEYGERFDgvrp--dplRVPREEIERARAalpGELAEAFRTAIENVRRFHERELdr--sW 105 Rubrobacter xyla...
YP_592758    27 VEPVVRKIVVDVRKNGDAALRRYASKLDglgk-taalRVSTEELKSAWDatrSEFKQAIRAAAKNIERFARWQMpk--sW 103 Acidobacteria ba...
P73058       29 ALAIAKEVIEQVKMEGDAGVLHYSRQFDfagataenlRVSEAEFAEAEKlvdPELRRAVEHAFRNIEKVHAGQMpppmhL 108 Synechocystis sp...
Q483H8       32 FIDIVKPIIENVKLNGDKALSEYAKKFDkaevstdqiQVTQAEFDEAFTlvdEEVIQTLSYSIDNIKKFHEAQMpeemwM 111 Colwellia psychr...
Q8TXG3       26 VLPDVERIFEDVVERGDEALLEYTERFDgvkleaedlRVSEDDFEVARElvdERTVEALEEAAHRIEEFHRKTLprvdrI 105 Methanopyrus kan...
Q987C6       31 FVEKVRPIIQAVKDEGDAALIRFARELDkanvaegglQVSEAEFDAAFDkveKDVVESIGFGIDNIRRFHEEQKpetmwL 110 Mesorhizobium lo...
ABM56000     42 YANAVLPIIEDVRNRGDAALVHYAAQFEgaaigldsiRVSPAEFDAAFAmldPKVVAAIEYGIENIRSFHTAQKpvqywM 121 Verminephrobacte...
ZP_02857082  33 FIDKVNPIIEAVRIDGDAALADFARQFDkaqvaadqlRATTEEFSAARAsidPELITTLEFAAGNIRHFHEKQMpetlvL 112 Rhizobium legumi...
Feature 1                                                                                       
1KAE_A      109 VETq-pGVRCQQVTRPVasVGLYIPGGsAPLFSTVLXLATPASIAGCKKVVLCSPpp-----iADEILYAAQLCGVQDVF 182 Escherichia coli
YP_825109   100 NRSfgaGLQLGQLVRPLdaVAAYIPAGrYPLPSTVMMTVIPAQVAGVPNICIACPk------pVAEVYGTAAMLGVRNVF 173 Solibacter usita...
YP_643880   106 ELEr-gGARLGQRVRPLrrAGIYVPGGhGAYPSTLVMTAVPAQVAGVEELCVCTPpgpd-graNPRLLAVAGLLGIGEVY 183 Rubrobacter xyla...
YP_592758   104 TRDagdGIELGQIIRPIesVGCYVPGGrYPLPSTVLMTVVPARVAGVERVVVVSPn------pARETLAAAYLLGVGEFY 177 Acidobacteria ba...
P73058      109 AEIe-pGVFAGEKITPLptVGLYVPRGkGAFPSMMLMLAVPARVAGVKKIVVCTPpdke-gkvEPVSLVTARMAGVDEVY 186 Synechocystis sp...
Q483H8      112 KQIr-pGCYAGDRFTPInaVACYIPRGkGSFPSVAIMTAVPAIVAGVPTAIIITPpgtd-gkvDAATLVVAKLVGIDKVF 189 Colwellia psychr...
Q8TXG3      106 TFDv-eGTECGLTLRPIprVGCYVPGGrAAYPSTALMTVIPARVAGCREVVVCTPpadndvraSPEVLVAVEIAGADAVY 184 Methanopyrus kan...
Q987C6      111 KEVr-pGAYAGDRYTPIasVALYVPRGkGAFPSVTMMTSVPAVIAGVPQIAIVTPptsd-gsvDAATLVAARLAGVHTVY 188 Mesorhizobium lo...
ABM56000    122 KELr-pGAFAGERITPIdtVALYVPRGkGSFPSTVMMLCVPAVVAQVPTAYILTPagpd-grvDAATLVAARLAGIETIF 199 Verminephrobacte...
ZP_02857082 113 HETh-rGVLVGDRWNAIdsVACYVPRGkGSFPSSVLMTAIPAKVAGVRKVVIITPpgpd-gtvDPATLVAAEIAGVSDVF 190 Rhizobium legumi...
Feature 1                                                                                       
1KAE_A      183 NVGGAQAIAALAFGtesVPKVDKIFGPGNAFVTEAKRQVSQrldgaaIDXPAGPSEVLVIADSGATPDFVASDLLSQAEH 262 Escherichia coli
YP_825109   174 QMGGAQAIAAFAFGtrtVPRADRIVGPGNIYVAAAKKLLAGev---gIDFIAGPTEILIIAADGDPRHFAADMLAQAEHD 250 Solibacter usita...
YP_643880   184 AAGGAQAVAAMAFGtesVPRVDKIVGPGNDYVTAAKLEVFGev---gVDAPQGPSELVVVADGSARPERVALDLMAQAEH 260 Rubrobacter xyla...
YP_592758   178 RIGGAQAVAALAYGtesVERVAKIVGPGNIFVTTAKKLVAFd----cAIDMLAGPTEAAIVSERGNARFIAADLVAQAEH 253 Acidobacteria ba...
P73058      187 KLGGVQALAAIAYGtktVSKVDKLIGPCSIYGAAAKRLLSGiv---dVGLPAGPSESIVLADETTDPKLAALDLLIEAEH 263 Synechocystis sp...
Q483H8      190 KCGGAQGIAAVAYGtntVPKCDKVVGPGSPFVVAAKKLLADii---hPGTPAGPSEAIVLADDTANPKLAALDLLVEAEH 266 Colwellia psychr...
Q8TXG3      185 RVGGAQAIAALAAGtetVLRVDKIVGPGNVYVTAAKLLAYSrgl-tdVDMPAGPSEVFVIADDSANPDWLARDLIAQAEH 263 Methanopyrus kan...
Q987C6      189 KCGGAQAVAAVAYGtetVKPALKIVGPGSPWVVAAKSELSSii---nTGLPAGPSEAIIFADDSVDGGLAALDLLIEAEH 265 Mesorhizobium lo...
ABM56000    200 KVGGSQAVAAAAFGtstIPKADKILGPGSPWVMAAKRALVDhl---dPGIPAGPSESIVFADASADARLAALDVLIEAEH 276 Verminephrobacte...
ZP_02857082 191 KCGGAQAIAAVAFGtqtVPKCDKVVGPGSPWVVAAKKQLSSli---dPGSPAGPSELIIFSDGSVPAELVALDLCVESEH 267 Rhizobium legumi...
Feature 1                                                                          ##           
1KAE_A      263 gpDSQVILLTPAADXARRVAEAVErqlaelpra----etarqalnaSRLIVTKDLAQCVEISNQYGPEHLIIQTRNARe- 337 Escherichia coli
YP_825109   251 v-DASAILLTTSKRLAAAVSRELErqla--------------ilptAPVASKSIAKNSAIILVRSLDEALEISNRFAPeh 315 Solibacter usita...
YP_643880   261 lsGATSVLLTPSGALASGVEPLLSgrp----------------sehITLVLVRDLEQALEISNLYAPEHVHLNCEGAGa- 323 Rubrobacter xyla...
YP_592758   254 dpETMVAFFTTSPSLADAVKKQVAlla-----------------akNTIARKALATRGYIFVAQDIAQAMEAANQFASeh 316 Acidobacteria ba...
P73058      264 gsDSAALLVTHSASLAEKALGYLGeyleklppwrk-kfcedglgsyGGILLTDSLQASLDFINDYAPEHLQVLTADPLk- 341 Synechocystis sp...
Q483H8      267 gpDSSAFLVTNSKELAEQAQVAINeywqhmdrlrvdfsstvlsgdnGGIVLTSTFEEAVDFCNDYAAEHLLILSKSPFd- 345 Colwellia psychr...
Q8TXG3      264 dpHAAAVLATDSEEIARAVKERVEelldagiere--eivlkaldrnGWIVVLDSLEECVRLANRYAPEHLQLCVENPEe- 340 Methanopyrus kan...
Q987C6      266 gpDSSAYLVTHSRKVAEAALAALPqhwsrmteqrvefsravltgkrGGIVLTASLEDSYRFINDYAPEHLEILSKEPFa- 344 Mesorhizobium lo...
ABM56000    277 gpDSSAFLVTTSRAIASAAIDWIAhfwnemsarrvefsatvltgprGGVLLADSVPSAMDFINDYAPEHLEILGAEPFg- 355 Verminephrobacte...
ZP_02857082 268 gpDSSVFFVTDNADFANAVASCVPalwsrmgniraqysktvlsgprGGIVIARTRADALAFVNDYAPEHLAVLADNAWq- 346 Rhizobium legumi...
Feature 1                                                                                       
1KAE_A      338 -------LVDSITSAGSVFLGDwSPESAGDYASGTNHVLPTYGYTAtCSSLGLADFQKRXTVQELSkegfsalastietL 410 Escherichia coli
YP_825109   316 lsipdasLLPGVRHAGSVFVGPySPEAAGDYASGPNHVLPTSGAARqRGGLSVTDYVKVISVQNLTepalrrlapsittL 395 Solibacter usita...
YP_643880   324 -------ALERVEAAGLVAVGDlSPVALSDYAAGPSHALPTGGTARwASALGTHDFLTRINHMRFSrealrelgphverL 396 Rubrobacter xyla...
YP_592758   317 lt-vdedLLETVSSAGSVFVGDySAQSFGDYASGPNHVLPTGGLARvRGGLSVLDYVKIITVQKISrkglralapiaetL 395 Acidobacteria ba...
P73058      342 -------LVGKIDNAGEILLGNyTPSSAATYAIGVNAVLPTGGFARsYSAVSVFDFLKRSTLAYLTeegfagvketvttL 414 Synechocystis sp...
Q483H8      346 -------HLGKIINAGEILLGEnTPISIANYTLGPNAVLPTSMAAKtASPLSVFDYLKSCSIGYLTregyeelaphtyrF 418 Colwellia psychr...
Q8TXG3      341 -------LLQDVENAGAVFVGHlTAVPFGDYATGPNHVLPTGGFARaRGALGTWDFVKIIPIQRLRegdverlapiveeL 413 Methanopyrus kan...
Q987C6      345 -------HLGHITEAAEILMGPhTPVTLANFVLGPNAVLPTSRWARtYGPLSVTDFVKRSSVGYVTsaaypelakharrL 417 Mesorhizobium lo...
ABM56000    356 -------YLHQIRNAGEILLGHaTPMSIANFVLGPNAVLPTSFGAKtVSALSVHDFIKRTSIGYVTtkafppmaamgreL 428 Verminephrobacte...
ZP_02857082 347 -------YLACFEHAGEILLGPhSAISVANFVLGPSHVLPTGGAAKtTSPLSVFDFLKRTSIASLSkeayggfaahaerL 419 Rhizobium legumi...
Feature 1                
1KAE_A      411 AAAERLTAH 419 Escherichia coli
YP_825109   396 ARAEGLEAH 404 Solibacter usitatus Ellin6076
YP_643880   397 ARMEGFENH 405 Rubrobacter xylanophilus DSM 9941
YP_592758   396 AAAEGLVAH 404 Acidobacteria bacterium Ellin345
P73058      415 ADYEDFPAH 423 Synechocystis sp. PCC 6803
Q483H8      419 AKYEGFDAH 427 Colwellia psychrerythraea 34H
Q8TXG3      414 AEREGLPNH 422 Methanopyrus kandleri AV19
Q987C6      418 ARYEGFSSH 426 Mesorhizobium loti MAFF303099
ABM56000    429 AIYEGFDAH 437 Verminephrobacter eiseniae EF01-2
ZP_02857082 420 ARYEGFDGH 428 Rhizobium leguminosarum bv. trifolii WSM2304

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