1M01


Conserved Protein Domain Family
GH20_SpHex_like

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cd06568: GH20_SpHex_like 
Click on image for an interactive view with Cn3D
A subgroup of the Glycosyl hydrolase family 20 (GH20) catalytic domain found in proteins similar to the N-acetylhexosaminidase from Streptomyces plicatus (SpHex). SpHex catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. An Asp residue within the active site plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. Proteins belonging to this subgroup lack the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases.
Statistics
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PSSM-Id: 119336
View PSSM: cd06568
Aligned: 14 rows
Threshold Bit Score: 404.793
Threshold Setting Gi: 117164684
Created: 22-Aug-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1M01_A; Streptomyces plicatus Beta-hexosaminidase bound with product (GlcNAc), contacts at 3.5A. N-acetyl-D-glucosamine
    View structure with Cn3D
  • Comment:For 1M01_A, the most C-terminal active site Asp residue plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    #                                                                    
1M01_A        157 YAWRSAMLDVSRHFFGVDEVKRYIDRVARYKYNKLHLHLSDDQGWRIAIDSWPRLAtyggst------------------ 218  Streptomyces ...
YP_001885639  190 YNYRGLMIDVVRHFFTIDEVKRQIDYAAQYKINRVHLHLSDDQGWRLEIKKYPDLTiiggst------------------ 251  Clostridium b...
ZP_02867813   189 YEYRGSHLDVTRHFFSVEDVKRYIDNMAQYKMNKLHLHLSDDQGWRLEIKGEMYGEslsklntiga-----------qts 257  Clostridium s...
ZP_01060593   165 FEYRGAMLDVARHFFTVEDVKKYIDLLAYYKYNVLHLHLTDDQGWRIEIKSWPKLTeigget------------------ 226  Leeuwenhoekie...
ZP_01050903   184 FAYRGMMLDVARHFFTVNQVKRVIDQMASYKLNKLHLHLTDDQGWRIEIKSWPKLTeiggss------------------ 245  Dokdonia dong...
ZP_01130457   148 FSYRGAMLDVARHFFDVATVKRHLDRMSLLKLNVLHLHLTDDQGWRIHIDSWPNLTalgatt------------------ 209  marine actino...
YP_871834     203 FAYRGAMLDVARHFFPVADVERYIDELALYKVNVLHLHLSDDQGWRIAIDSWPKLApvggkt------------------ 264  Acidothermus ...
YP_946719     138 FAYRGLMLDVARNFFTVQEVKEQIDVMTQFKFNALHLHLTDDQAWRIEIHEPAKNPsglpyakltevggq---gavevst 214  Arthrobacter ...
CAB72127      487 FDKRGMMLDVAREFKNPDEVKAIIDSLASYKISTLHMHLADDQGWRIEITNEGKVAgddidynqlteisgkggmtqfnrt 566  Arthrobacter sp.
YP_001301467  178 FAWRGLSFDVSRCFFDPEEVKQVIDMIALYKMNVLHMHLSDNQGWRIEIKKYPELAeiggql------------------ 239  Parabacteroid...
Feature 1                                               #                                         
1M01_A        219 -evgggpGGYYTKAEYKEIVRYAASRHLEVVPEIDMPGHTNAALASYAELncdgvapply---------tgtkvgfssLC 288  Streptomyces ...
YP_001885639  252 -evgggkGGYYTQEEFKDLVKYAAERYVEIIPELDMPGHSNAALASYGFLnkdgekkply---------tgtevgfssLM 321  Clostridium b...
ZP_02867813   258 tsingikAGQYTQEDYKEIVAYAADRYVEIIPEFDMPGHSWAALVSLNFLnssedgkphsgnydntkpyegidvgfstFE 337  Clostridium s...
ZP_01060593   227 -evgnepGGFYTQEDYKAIVAYAAQHHMTIVPEVDMPGHTNAASVAYPFLngngktpkiy---------rgtqvgfstFD 296  Leeuwenhoekie...
ZP_01050903   246 -svrdesPGFYTQEDYKEIVAYAQSKYITVIPEIDMPGHTNAALASYPELncngkatkly---------rgmrvgfstLC 315  Dokdonia dong...
ZP_01130457   210 -avgggnGGFYSKADYTEIVEFAASRYITVVPEIDVPGHTNAALSAYPELncddvapapy---------tgievgfssLC 279  marine actino...
YP_871834     265 -evgggpGGYYTQADYRAIVAYAQAHFITVVPEIETPGHVNAALVAYPQLacsgkpirpy---------tgtgvgfssLC 334  Acidothermus ...
YP_946719     215 ampprgnSGFYTQQDFKDIQAYAATKNVLVIPEIDLPGHVNAALAAIPQLnpdgqakpms---------ttsevgwstLT 285  Arthrobacter ...
CAB72127      567 ymdllgnTGFYTQAEYKDLVAYAADRHIEIIPEIDVPGHTSAILHAIPQLntagtkpnvdewgv-vpedgtgnvgtstLD 645  Arthrobacter sp.
YP_001301467  240 -pnngrkGGYYTQEEFKDLVNYAKERFITIIPEVDIPGHTAAIFAAYPDFknavkfktnv---------nipgqafnaLD 309  Parabacteroid...
Feature 1                                       ##                                                
1M01_A        289 VDKDVTYDFVDDVIGELAALTpGRYLHIGGDEAhstpKADFVAFMKRVQPIVAKygKTVVGWHQlagae--pvegALVQY 366  Streptomyces ...
YP_001885639  322 THNEETYEFIDNVIKEVSEISpSKYIHIGGDEAdstkKSDYDYFVGRVSKIIEKygKTPIGWDPidisp-einssVILQN 400  Clostridium b...
ZP_02867813   338 CRNEKTYEFIDEVFRQVAAISpSKYIHIGGDEAhstsSEDYAYFMNRVTEIAKKygKTPIGWQNydgva-edkegTVTQF 416  Clostridium s...
ZP_01060593   297 TRKDTVYSFIDDVVREISAITpGPYFHIGGDEShvtqKDDYIYFVNKVEQIVQKydKKMIGWDEvatad--idttSIAQF 374  Leeuwenhoekie...
ZP_01050903   316 VDKEITYKFIDDVIREVTEITpGEYIHLGGDEShvtsKKDYNIFLNKVFPIVKKygKSVVGWEEiqsan--idstYVIQH 393  Dokdonia dong...
ZP_01130457   280 IDKDVTYQFLDDVFREVSELTpGPYLHLGGDESlatsDEDYLEFIRRATAIAAShgKALIGWHEmgrsr-dlpagTVGQY 358  marine actino...
YP_871834     335 ISNPTVYQFVDDVVGELAALTpGPYIHLGGDEAmstpPSEYAAFVQKAQAIVEAhgKTLMGWAEiakgs--ldasAVAEY 412  Acidothermus ...
YP_946719     286 EDLPATYEFVREVLGQLAAITvGPYLHIGGDEAhvtgHDDYVSMVQRFVRIGAEtgKSVVGWNEfaave--lpqgSVIQY 363  Arthrobacter ...
CAB72127      646 VAAPQTWTFLEHVFGQIAEMTtSEYIHIGGDEShvtgHDNYVEFITKAVKLIHDldKKPIGWNEvaiggleaggrHSVLD 725  Arthrobacter sp.
YP_001301467  310 VDDPKAMQFTEDVIAELAALApGNYIHIGGDEAiglpHDKFVRFINKTREIVLKngKKMVGWQEtarad--isegDVIQH 387  Parabacteroid...
Feature 1         #                                                         # #                   
1M01_A        367 WGldrtgd--------------------------aekaeVAEAARNGTGLILSPAdrtYLDMKYtkdtplgl-------- 412  Streptomyces ...
YP_001885639  401 WKd-----------------------------------sNEAARKKEMKMIISIAqkaYLDMKYnestpygl-------- 437  Clostridium b...
ZP_02867813   417 WSt------------------------------------GNAKLKDGIKYVVSPAdyaYMDMKYdascelgl-------- 452  Clostridium s...
ZP_01060593   375 WQsq---------------------------------knAELAVAKNLKVIMSPAkkaYLDMKYdlkseygl-------- 413  Leeuwenhoekie...
ZP_01050903   394 WQke---------------------------------atAQKGIDKGAKVILSPAkkmYLDMKYtklspigl-------- 432  Dokdonia dong...
ZP_01130457   359 WSyvaprd--------------------------dadkrVASFIEQGGQMIMSPAdaiYLDMKYasdeelgl-------- 404  marine actino...
YP_871834     413 WNfrdg------------------------------masARQALARGMRLVAAPAdhaYLDQKYtatsrlgl-------- 454  Acidothermus ...
YP_946719     364 WHgdf-------------------------------eptVRQAEQNGSRVIMSPAantYLDQKYasdspvgle------- 405  Arthrobacter ...
CAB72127      726 RRhr---------------------------------rhAEGHQDKGAKLMVSNGstaYLDMKYnaktpigl-------- 764  Arthrobacter sp.
YP_001301467  388 WIylkqknedsskkkdnipaeykeimalfaefmkeapkdPGLGISKKAKVILSPSgyvYLDHKYlepsadstqnaeqerl 467  Parabacteroid...
Feature 1                                                  # #                                    
1M01_A        413 --swagyveVQRSYDWDPAgylpg----apadaVRGVEAPLWTETLsdpDQLDYMAFPRLPGVAELGWSpasthDWDTYK 486  Streptomyces ...
YP_001885639  438 --twagyipVETAYKWDPTdyap-------kelVLGIEAPLWTETIsdaKAMDYMIYPRLLGYAEIGWTpkenrDWSQYK 508  Clostridium b...
ZP_02867813   453 --qwagynsIEDTYSWDPTnygd-------kenIVGIEACLWSETLannDHLDYMAYPKILSHAEIGWTpkelrSWDTYK 523  Clostridium s...
ZP_01060593   414 --nwagyvpVNTAYVWKPEayeni-----prehILGIEAPLWSETIstiSELEYLAFPRAIGYAELNWSqpeqrDWENYK 486  Leeuwenhoekie...
ZP_01050903   433 --twagmveVDSAYIWQPSsifkd----idtsqLLGLESPLWAETIqtsDDIEYLAFPRVIGHAELGWSnpanyNWDNYR 506  Dokdonia dong...
ZP_01130457   405 -ewadgpttLHDAYTWDPAaitig----vtdehILGIEAPLWTETIdnvRDLEYMVFPRIIAAAEIAWSprsahDWADFT 479  marine actino...
YP_871834     455 --twagpvsVAEAYAWDPTmiap-------dgdVLGVEAPLWSETIrtmADIEYLAWPRMAGIAEIGWTpqserSWQEYR 525  Acidothermus ...
YP_946719     406 -wveggpftWSEYYNWDPAqggl------qdqqILGVEGPLWTETVrnnQQAQWLLYPRAVSLAEVAWSgqevrNPGDFR 478  Arthrobacter ...
CAB72127      765 --twagmgdFPKYYDWNPAavvkdgttnlpdsaILGVEAPQWSETIrggKQTEFMVFPRVISFAEVGWTpqakrNVSDFK 842  Arthrobacter sp.
YP_001301467  468 gmaayekqtIQEMYDWDPMtfnptv--enpqkdVAGIEAAIWCETItnfRDLQFLLMPRLAGVAEKGWSkventHWDEYK 545  Parabacteroid...
Feature 1            
1M01_A        487 VRL 489  Streptomyces plicatus
YP_001885639  509 NRL 511  Clostridium botulinum B str. Eklund 17B
ZP_02867813   524 PRL 526  Clostridium spiroforme DSM 1552
ZP_01060593   487 VRL 489  Leeuwenhoekiella blandensis MED217
ZP_01050903   507 VRL 509  Dokdonia donghaensis MED134
ZP_01130457   480 RRL 482  marine actinobacterium PHSC20C1
YP_871834     526 LRL 528  Acidothermus cellulolyticus 11B
YP_946719     479 RRL 481  Arthrobacter aurescens TC1
CAB72127      843 VRM 845  Arthrobacter sp.
YP_001301467  546 VRL 548  Parabacteroides distasonis ATCC 8503

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