2EPL


Conserved Protein Domain Family
GH20_GcnA-like

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cd06565: GH20_GcnA-like 
Click on image for an interactive view with Cn3D
Glycosyl hydrolase family 20 (GH20) catalytic domain of N-acetyl-beta-D-glucosaminidase (GcnA, also known as BhsA) and related proteins. GcnA is an exoglucosidase which cleaves N-acetyl-beta-D-galactosamine (NAG) and N-acetyl-beta-D-galactosamine residues from 4-methylumbelliferylated (4MU) substrates, as well as cleaving NAG from chito-oligosaccharides (i.e. NAG polymers). In contrast, sulfated forms of the substrate are unable to be cleaved and act instead as mild competitive inhibitors. Additionally, the enzyme is known to be poisoned by several first-row transition metals as well as by mercury. GcnA forms a homodimer with subunits comprised of three domains, an N-terminal zincin-like domain, this central catalytic GH20 domain, and a C-terminal alpha helical domain. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
Statistics
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PSSM-Id: 119335
View PSSM: cd06565
Aligned: 44 rows
Threshold Bit Score: 267.15
Threshold Setting Gi: 182412524
Created: 15-Nov-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative active
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 #                                                                      
2EPL_X        86 DLAYMADCSRnAVLNLSSAKKMIEVLALMGYSTFELYMEDTYEIENQPYFgyfrgRYTVAELQEIEDYAADFDMSFVPCI 165 Streptococcus g...
ABB20818      22 FRGFMLDVSCdRVPTRSTLDFLTRVMVRCGLNHLELYGEAGFAYRGEEEVwadrsPVTAEDMRWLDGLCRGRGIELGANQ 101 Propionibacteri...
NP_695287    184 VRAYSLDVTRgRVPTMAFLTWFIDQLALYKYNQFQLYVEHAFAFGELSEAwrgtdPLTADDIMFLDEYCAHHGIELVPSL 263 Bifidobacterium...
YP_920698    101 FRGFHLDVARgGVATVEELKRLLRWLFLLKYNYLAVYVEDLFPWDRYPDIgarrgRYTGKEWREVVEYGGRLGVEVFPSL 180 Thermofilum pen...
NP_623505     99 FRGYHLDIARgGVPNLDTFKKILRWLFLLKYNYFGIYFEDLFPWQKYPQIgklrgRLSREELEEIISYGENLGIEVFPSL 178 Thermoanaerobac...
YP_001181303  97 FRGFHLDIARgGVPSVSTFKNILRWLFLLKINYFAIYFEDLFPWEKHPKIgagrgRLTKEELKEIIEYGKNLGIEVFPSL 176 Caldicellulosir...
YP_074133    160 RRGVMLDISRgKVPTMESLYRFVDLLADLKINEFQLYTEHTFAYREHREVwqaysPMTGEEIMLLDRYCRERFIDLVPNQ 239 Symbiobacterium...
BAB80687     131 YRGFYHDVTRgMVPTLDTLKSLVDKAAFYKINQLQLYIEHTFAFKGMSEVwmdkdPLTAEEILILDKYCKERHVELVPSL 210 Clostridium per...
ZP_03016090  155 YRAVHFDVKH-HLDRTEYYYRAIDRLARYKINAVIWELEDKLRYTRRPEVaa-gnAISKQEMQAICRYAQERNVEISPLV 232 Bacteroides int...
ZP_01721340  144 YRAVHLDVKH-HLEKKEYYYELMDRLAELKINAIIIEIEDKLGYELQPVVas-qdAYSIDEWKAISDYAMARNIRISPLV 221 Algoriphagus sp...
Feature 1            #                                                                     ##    
2EPL_X       166 QTLAHLSAFVKWgikEVQELRDve---------------diLLIGEEKVYDLIEGMFQTMAHLHt--RKINIGMDEAHLV 228 Streptococcus g...
ABB20818     102 NCLGHMEPWLETe--SHHHRCEnpdgvdlp---wgvharasTLAPVPENLDFVQRLLGELTETVs-sKFLNVGLDEPWEL 175 Propionibacteri...
NP_695287    264 ATFGHMYMNLRTr--EHRGLGEfpedadrpfsfiermehhtLNAANPKSHDFASRLIEEYAPLFr-sRSFNIGGDETFDL 340 Bifidobacterium...
YP_920698    181 ELAGHMENILSLp--GYRRFSEwhrp-----------eegcLDVGDPEARRFAEELLEEALEKTk-sRYIHIGGDETWAM 246 Thermofilum pen...
NP_623505    179 ELCGHMENILSLp--EFTRFSEwhrp-----------qegtINVFDEEAREFTYDLLREVVEFFp-sEHIHIGGDETWAL 244 Thermoanaerobac...
YP_001181303 177 ELTGHMENILSIp--EYSKYSEwylp-----------regcLDLSSEEAKRFAYELLEEVLEFFp-sKYVHIGGDETWAL 242 Caldicellulosir...
YP_074133    240 NSFGHMTPWLIHs--RYKHMAEapdgfelpw-ggrrnepfsLSPAEPSVISFLAGLYDELLPHFt-sQYFNVGCDETWDV 315 Symbiobacterium...
BAB80687     211 STFGHLYEALRSk--SFRELCEleigdeeys-fvdrmahhtLDVTNPKSLDFVESMLLEFIPLFs-sDKFNICCDETFDL 286 Clostridium per...
ZP_03016090  233 QGLGHAGFILKHh-wELRENPSsd---------------weFCPTNPKTYEFQFDLYRDALEAMpygRFLHVGGDETMAI 296 Bacteroides int...
ZP_01721340  222 QGLGHASFILKHe--EYAALRDdpas------------dwaFNPLDDKTYDVQFDLYADALKAFphaDYLHVGGDEVHTT 287 Algoriphagus sp...
Feature 1                                              #                                         
2EPL_X       229 GLGrylikhgfqnrSLLMCQHLERVLDIADKYGFNCQMWSDMffklmsadgqy-----------drdveipeetrVYLDR 297 Streptococcus g...
ABB20818     176 GTGvsadrcrdegkGVVYAKWARDVVTPWLDRGWRVAMWADIvns----------------------------hpEALDI 227 Propionibacteri...
NP_695287    341 GRGrsvqdspgasrDELYADFVKDLCSTLAHRGLQPMLWADIale----------------------------npHTMDL 392 Bifidobacterium...
YP_920698    247 GRGrsldrtlrfewPRLYAEHHSRLLRLARERGKTPLIWGDMlagmylre-----------------terdlwrpILENP 309 Thermofilum pen...
NP_623505    245 GRGksldktntfkgPELFELHHRNLINIVRDKGKTPIVWGDMltgmylrp-----------------derekwkvVLESE 307 Thermoanaerobac...
YP_001181303 243 GRGksleknwifegPKLYEEHHKNMIDMVEKYRKIPIMWADMltgmflrp-----------------derkvwenLLQSD 305 Caldicellulosir...
YP_074133    316 GQGrsaravaergeHEVYLEQLLKIHRLVAERRRTMQFWGDIiik----------------------------ypEAVAR 367 Symbiobacterium...
BAB80687     287 GKGksrekaeklgvGKIYTEFLNKVYNIVKSFNKNVMFWGDIivg----------------------------ypELLSD 338 Clostridium per...
ZP_03016090  297 GIDercka-tdksaFELQMGWLRKVCDFAKSNGRIPIFWDDMplkygglwdmahgd-----ndtddlhwdtqkldSVIDL 370 Bacteroides int...
ZP_01721340  288 GRGsgm------spLELQLTWLNRVSKYAEDNGLTPIFWDDMplknaglygsiydsklskaqadsiwaaneyklnEYVDM 361 Algoriphagus sp...
Feature 1                 #                                                                      
2EPL_X       298 LKERVTLVYWDYYQDseek---------------ynrnFQNHHKISQDIAFAGGAWKWIGFtPHNHFSRLVAIEAnkacr 362 Streptococcus g...
ABB20818     228 IPDEVLLIPWTYESPameakfagtegyeirsdagfastARLVANAGRPFVNAPGTGAWNTFtGRLSNAVGNIVDAvitdh 307 Propionibacteri...
NP_695287    393 LPGDITMLNWMYEPDide------------------skIQTIASQGRRQFVCPAVRAWSRFfPDYDGAWLNTYRMavagl 454 Bifidobacterium...
YP_920698    310 AWREAVVANWDYSPGtvey---------------fkqkIRLFKERGYEQIVCPGLWNWDRYyPDFDAALANVKSFlqaar 374 Thermofilum pen...
NP_623505    308 IWDDVIIANWDYTNLsekh---------------frekINLFGKRKEKQIVCPGFSNWGRFyPSFDIAITNITNFlkpak 372 Thermoanaerobac...
YP_001181303 306 IWQRTILANWDYAAMpkehf--------------inriESLGKNYQSNQIVCPGFSNWNRFyPDFEAAIENIKNFidaak 371 Caldicellulosir...
YP_074133    368 LPKDVIALEWGYEADhpf-----------------dehCAVYAHAGVPFYVCPGTSTWMSLvGRTENALQNMRSAaesgl 430 Symbiobacterium...
BAB80687     339 IPEDLTCLTWNYHPQand------------------vaTKIIAEHNKVQYVCPGVGGWNMMmNLIEGSFSNIRRMvnhgi 400 Clostridium per...
ZP_03016090  371 FPKDCVYMRWNYRDAtrpgh--------------qrilKWYHDKGLRVMAATAASSGNSPVlPREDTKAGYIKGFsrlva 436 Bacteroides int...
ZP_01721340  362 FPKNCVYMRWNYSLPespgn--------------nlaiQWFTKNGFNVMGATAGQTRWNLM-PLENSNTQNIRDFanisi 426 Algoriphagus sp...
Feature 1                   # #                        
2EPL_X       363 knqVKEVIVTGWGDNGGETSq-fSVLPALQIWAELAYR 399 Streptococcus gordonii
ABB20818     308 slgGKGVLLTGWGDSGHHAGlvfSLVPLVVTGVAMADP 345 Propionibacterium acnes
NP_695287    455 kygAEGMVVTDWGDYGHVNDp-rLSVPGLCYGAQNAWN 491 Bifidobacterium longum NCC2705
YP_920698    375 eegVKGFMVTAWGDDGEECLy-sFLYPLILASMEYAEG 411 Thermofilum pendens Hrk 5
NP_623505    373 eegVKGFLVTSWGDDGAEALy-sFLDPLILANMEIAEG 409 Thermoanaerobacter tengcongensis MB4
YP_001181303 372 ekgIQGFLVTSWGDDGQECLf-sFLYPLLVATIEFAEG 408 Caldicellulosiruptor saccharolyticus DSM 8903
YP_074133    431 khgAQGFLNTIWGDLGHQDYepvAYLPVAFGAAVSWAL 468 Symbiobacterium thermophilum IAM 14863
BAB80687     401 kygAIGVLNTNWGDYGNINLl-aNSIPSMIYGAGISWN 437 Clostridium perfringens str. 13
ZP_03016090  437 qnqLEGILATSWDDGSPHLE---TVWRGYIAQGEFGWN 471 Bacteroides intestinalis DSM 17393
ZP_01721340  427 nnkADGLLLTLWDDDSPLFE---LYQRGIATFSEYTWS 461 Algoriphagus sp. PR1

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