1C7T,1M01


Conserved Protein Domain Family
GH20_chitobiase-like

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cd06563: GH20_chitobiase-like 
Click on image for an interactive view with Cn3D
The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
Statistics
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PSSM-Id: 119333
View PSSM: cd06563
Aligned: 60 rows
Threshold Bit Score: 384.62
Threshold Setting Gi: 10835639
Created: 16-Nov-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1M01_A; Streptomyces plicatus Beta-hexosaminidase bound with product (GlcNAc), contacts at 3.5A. N-acetyl-D-glucosamine
    View structure with Cn3D
  • Comment:For 1M01_A, the most C-terminal active site Asp residue plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.
  • Structure:1C7T_A; Serratia marcescens chitobiase binds Di- N Acetyl-D-Glucosamine, contacts at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                   #                                                                    
1C7T_A       311 FPYRGIFLDVARNFHKKDAVLRLLDQMAAYKLNKFHFHLSDDEGWRIEIPGLPELTevggqrchdlsettcllpqygqgp 390 Serratia marces...
1M01_A       157 YAWRSAMLDVSRHFFGVDEVKRYIDRVARYKYNKLHLHLSDDQGWRIAIDSWPRLAtyggst---------------evg 221 Streptomyces pl...
NP_798865    260 FRYRGMMLDCARHFHSVEQVKRLINQLAHYKFNTFHWHLTDDEGWRIEIKSLPQLTdigawrgldetn----epqythla 335 Vibrio parahaem...
YP_128743    265 YSYRGMMLDCSRHFHSTKRIKHLLDQLARYKFNTFHWHLTDDEGWRIEIDAYPELTnigawrgpneei----qpqfttid 340 Photobacterium ...
YP_001142849 245 FGFRGIFLDCARHFHPVANLKRLLKLMSLYKFNRFHWHLTDDEGWRLEIKAFPQLTeiaawrghdlpv----gpqlsggp 320 Aeromonas salmo...
YP_001197126 151 FEWRGMMLDCSRQFFDKQTVKNYIDWLAAHKMNVFHWHLTDDNGWRIEIKSMPDLTlkgawrgpgevl----lpsygsgd 226 Flavobacterium ...
ZP_01546465  271 FSWRGTHLDVSRHFRGPKDILRLLDILAWGRMNVFQWHLTDDEGWRLEIKAYPELTvsgarrgpgceq----vpqlgfaa 346 Stappia aggrega...
YP_472553    161 MGWRGLHLDVARQFYGVAEVKKLLAVLAWNKLNRFHWHLSDDEAWRVEIDAYPALTeigawrghglav----ppllgssp 236 Rhizobium etli ...
YP_614733    264 HGWRGAHLDVSRQFYPLDQVLRYVDIMAWHKMNRFHWHLTDDEGWRLEIKAYPQLTetaahtgmdlpv----lpqlgpdm 339 Silicibacter sp...
ZP_01156495  229 FPWRGQHLDCARHFYEPHTIRRLMDLMALLKMNRFHWHFADDEAFRLEVTCFPDVWkrtcvrgeget-----ipgvfggg 303 Oceanicola gran...
Feature 1                                          #                                             
1C7T_A       391 dvyGGFFSRQDYIDIIKYAQARQIEVIPEIDMPAHARAAVVSMearykklhaagkeqeanefrlvdqtdtsnttsvqffn 470 Serratia marces...
1M01_A       222 ggpGGYYTKAEYKEIVRYAASRHLEVVPEIDMPGHTNAALASYaelncdgvapp-------------------lytgtkv 282 Streptomyces pl...
NP_798865    336 eryGGFYTQEDIKDVVAFASKRGITVIPEIDVPGHCRAAIKSLphllveaedtte------------------yrsiqhy 397 Vibrio parahaem...
YP_128743    341 qryGGFYSKAEVRDLIAYAHDRGITIIPEIDIPGHCRAAIKSLptllidpndhsv------------------yrsiqgy 402 Photobacterium ...
YP_001142849 321 epyGGYYTQSEVRELVAYASELGITVIPEIDIPGHCHAAIKALpgllieeadrsr------------------ylsvqyf 382 Aeromonas salmo...
YP_001197126 227 kryGGFYTQEHIKEVVAYAANRGISVMPEIEIPGHSRAVTASYpevgcaitqel--------------------ksvqge 286 Flavobacterium ...
ZP_01546465  347 evyEGAYSQDEVREIVAHAASLNIDILPEIDVPGHSTAVLKTYprfadqaeapes------------------yhsvqgy 408 Stappia aggrega...
YP_472553    237 trtGGYYTKSVIREIVAHAKSFGIEIVPEIDMPGHCYAMQQAIpelrdpaekgs-------------------yysvqgf 297 Rhizobium etli ...
YP_614733    340 tgqSGFYTQDEARQVVKHAAQFGIEVMPEIDVPGHCACVLGALpdlvdpeepes-------------------ywsvqgf 400 Silicibacter sp...
ZP_01156495  304 vrsGGSYSLETARDVVAHGQALEIGVLPEIEVPAHALALARVMpelrdpsdtgq-------------------ersvqgy 364 Oceanicola gran...
Feature 1                                                 ##                                     
1C7T_A       471 rqsyLNPCLDSSQRFVDKVIGEIAQMHkeagqpIKTWHFGGDDAknirlgagytdkakpepgkgiidqsnedkpwaksqv 550 Serratia marces...
1M01_A       283 gfssLCVDKDVTYDFVDDVIGELAALTp-----GRYLHIGGDEAhs---------------------------------- 323 Streptomyces pl...
NP_798865    398 ndnvINPALPGSYEFIDKVLEEVSALFp-----APYVHIGADEVpngvwskspacq------------------------ 448 Vibrio parahaem...
YP_128743    403 sdnvLSPALEGTYTFISTILNEICDLFp-----APYIHIGGDEVpkgvwtdsegcq------------------------ 453 Photobacterium ...
YP_001142849 383 ddnvLNPALPGTYQFLEAVIDEVCELFp-----GPLVHMGGDEVptgvwtdspacq------------------------ 433 Aeromonas salmo...
YP_001197126 287 vknvWCVGREENYVLLDSIIREVSGLFp-----FEYIHVAGDEVnranwencpkcq------------------------ 337 Flavobacterium ...
ZP_01546465  409 pnnaLNPAMHETYEFLEKVFAEVASLFp-----FEFIHIGGDEVdvnswlespkaq------------------------ 459 Stappia aggrega...
YP_472553    298 pdncINPAREQTYEIVETILAELIELFp-----FKVIHLGADEVplgawsgspaalarlrdvageava-dahakrlnvvt 371 Rhizobium etli ...
YP_614733    401 annaLNPAIEESYTFAETVLAEVCEIFp-----FEVVHVGGDEVaegawmqspkaq------------------------ 451 Silicibacter sp...
ZP_01156495  365 aenvLNPARDQFWTVIEQLSEEVAALFp-----LGMLHLGCDELpegawegspava------------------------ 415 Oceanicola gran...
Feature 1                                              #                      #                  
1C7T_A       551 cqtmikegkvadmEHLPSYFGQEVSKLVKAhgidRMQAWQDglkdaesskafatsrVGVNFWDtlywg--gfdsVNDWAN 628 Serratia marces...
1M01_A       324 -----------tpKADFVAFMKRVQPIVAKyg-kTVVGWHQlagae------pvegALVQYWGldrtgdaekaeVAEAAR 385 Streptomyces pl...
NP_798865    449 --almeqlgysdyKELQGHFLRHAEDKLRKlg-kRMLGWEEaqhgd-----kvskdTVIYSWLse-------eaALNCAR 513 Vibrio parahaem...
YP_128743    454 --qlmqqhgyqnpIELQGHLLKFAEGIIQAkg-kRMMGWEEvtkgd-----kvskdTMIFSWQne-------daGLISAQ 518 Photobacterium ...
YP_001142849 434 --qlmqrqgyqdyRELQGHLLRHCQQYLASkg-kQMLGWEEilhgd-----kvsrdATIFAWTsf-------qaGLDAAA 498 Aeromonas salmo...
YP_001197126 338 --almvkegftdsFQLQNYFFRRVQKIVDKyh-kKTDGWNEilkgg-----eidpnTLISAWQgi-------syGIESAK 402 Flavobacterium ...
ZP_01546465  460 --rlmdekgladtMEVQAYFMGRVRGILKKln-rKLAGWDEvshggg----idpdgVLLMAWQkq-------evTKDLID 525 Stappia aggrega...
YP_472553    372 nrhgaddihgsgaAILQAEFLERVQRFLASkg-cITGGWEEaahgdv----idkekSYLCSWRnv-------evSAELAE 439 Rhizobium etli ...
YP_614733    452 --ammretglkdtPQLQAYFLRHIQTYLAGlg-rKLGGWEEvahggg----ldpehSLLFAWTti-------ekTAELAQ 517 Silicibacter sp...
ZP_01156495  416 --dlkareglesaDDVSGWTMAKLAGHLSErg-vRVAAWEEaargsn---ggighgALLQSWSgq-------gpGLEAAR 482 Oceanicola gran...
Feature 1                     # #                                                                
1C7T_A       629 KGYEVVVSNPdyvYMDFPyevnpdergyywgtrfsdERKVFSFAPDnmpqnaetsvdrdgnhfnaksdkpwpgAYGLSAQ 708 Serratia marces...
1M01_A       386 NGTGLILSPAdrtYLDMKytkdtplg--lswagyveVQRSYDWDPAgylpg-----------------apadaVRGVEAP 446 Streptomyces pl...
NP_798865    514 QGFDVVLQPAqttYLDMTqdyapeepg-vdwanplpLEKTYNYEPLaevpad---------------dpirkrIWGIQTA 577 Vibrio parahaem...
YP_128743    519 QGYDVIMQPAqytYLDLAqgfsadepg-vdwagkvpLETVYSYQPFsklste---------------dpahqrIIGTQAG 582 Photobacterium ...
YP_001142849 499 AGYPVVMAPAqhlYLDLAwsqdprepg-lywagtlnLEQVYRCDPApadfh------------------andnILGVLSP 559 Aeromonas salmo...
YP_001197126 403 KGYQTIMMPGqytYFDMAqsetergh---rwaaitdTKRAYSFEPIptddlt---------------peqqknIIGVQGA 464 Flavobacterium ...
ZP_01546465  526 QGYDVICNPGqhyYMDMAqasgwqepg-agwagvstPQDCYTYEAStglsa-----------------gseqrLKGVQAC 587 Stappia aggrega...
YP_472553    440 RGYQMVVCPGqvyYLDMAlrpdwdepg-aswagnsdAEKLYKFDPLsgwta-----------------aqkqkLLGIQAC 501 Rhizobium etli ...
YP_614733    518 EGYDVISTPGqayYLDMAlsdawyapg-aswagftpLDKTYAFEADngdpv------------------lqgrLKGVQAC 578 Silicibacter sp...
ZP_01156495  483 AGYDVIMSPAqhvYLDMAhsddpddwg-aswaafvaLEDVIAWSPVppear-----------------diaerIKGVEGC 544 Oceanicola gran...
Feature 1         # #                                                                
1C7T_A       709 LWSEtqr-tdPQMEYMIFPRALSVAERSWHragweqdyragreykggethfvdtqalekDWLRFANIL 775 Serratia marcescens
1M01_A       447 LWTEtls-dpDQLDYMAFPRLPGVAELGWSpas------------------------thDWDTYKVRL 489 Streptomyces plicatus
NP_798865    578 LWCEiin-nqSRMDYMVFPRLTAMAEACWTdkq------------------------hrDWTDYLSRL 620 Vibrio parahaemolyticus RIM...
YP_128743    583 LWCElin-nqSRFEYMLFPRLLAIAEVCWSqpk------------------------qrNWDDFKARL 625 Photobacterium profundum SS9
YP_001142849 560 LWSElvt-srDRLDYMLFPRMLATAEVAWSdat------------------------rkAWDHFAARL 602 Aeromonas salmonicida subsp...
YP_001197126 465 LWSEyldrpaRIMEYQSYPRISALSEIGWSkke------------------------dkNWEDFYGRL 508 Flavobacterium johnsoniae U...
ZP_01546465  588 IWCEhmt-dnVIFNHMVFPRLYAVAEAGWTkpt------------------------nkDWQRFAAQA 630 Stappia aggregata IAM 12614
YP_472553    502 IWSEpmt-drAVFDRLVFPRISGLAETGWTkps------------------------akSWDRFKALA 544 Rhizobium etli CFN 42
YP_614733    579 VWSEhlt-tmARRNHMIFPRLSAIAEAGWSaae------------------------nkAYDRFKSLA 621 Silicibacter sp. TM1040
ZP_01156495  545 FWSEftt-hdREMEAMVAPRILGVAAKGWDitd------------------------rlTGAQLRGLA 587 Oceanicola granulosus HTCC2516

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