2DLA,1ZT2


Conserved Protein Domain Family
PriL

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cd06560: PriL 
Click on image for an interactive view with Cn3D
Archaeal/eukaryotic core primase: Large subunit, PriL
Primases synthesize the RNA primers required for DNA replication. Primases are grouped into two classes, bacteria/bacteriophage and archaeal/eukaryotic. The proteins in the two classes differ in structure and the replication apparatus components. The DNA replication machinery of archaeal organisms contains only the core primase, a simpler arrangement compared to eukaryotes. Archaeal/eukaryotic core primase is a heterodimeric enzyme consisting of a small catalytic subunit (PriS) and a large subunit (PriL). Although the catalytic activity resides within PriS, the PriL subunit is essential for primase function as disruption of the PriL gene in yeast is lethal. PriL is composed of two structural domains. Several functions have been proposed for PriL, such as the stabilization of PriS, involvement in the initiation of synthesis, the improvement of primase processivity, and the determination of product size.
Statistics
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PSSM-Id: 143473
View PSSM: cd06560
Aligned: 49 rows
Threshold Bit Score: 53.1618
Threshold Setting Gi: 45357572
Created: 8-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
PriL-PriS
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:PriL-PriS interface [polypeptide binding site]
Evidence:
  • Comment:Archaeal/eukaryotic primases form a heterodimer consisting of a small catalytic subunit (PriS) and a large subunit (PriL).
  • Structure:1ZT2_D; Sulfolobus solfataricus PriL interaction with PriS, defined at 4.0 A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
2DLA_B     24 DFMDAIPKIv---sVDDVIERIRVVkneklidk------------------fldqdnvmDLAQFYALLGALSYsp----- 77  Pyrococcus horikoshii
1ZT2_D     26 TLTDLLLNStt--lIDQAKDRIQKTksgdelp--------------------hyvsynePVLVFYTTLLSLAIln----- 78  Sulfolobus solfata...
Q58112     60 TIFDKIFQMidydiDGEVEKRLPKDesrfmigvr----------------rekeieikkEIITNLLDFLLIILls----- 118 Methanocaldococcus...
O26686     45 NLADNSLLPas--iAELAVKRLEWYlrrqrkdfnhrd----------yaylmnpeieeyDVLAFYILAQAAGAgfm---- 108 Methanothermobacte...
YP_447146  43 SQKLSSIPEt---lEQLALHKFQWYldkktdnfddkv----------yeylfnpeiyeyDVLSFYFLCQAVAIgfg---- 105 Methanosphaera sta...
Q41UX8     26 LVPENRSSVreafeYLNGVVRKYADnegy--------------------------nitmGKNAFAIAMWMLRYin----- 74  Ferroplasma acidar...
Q6L0D5     23 KIDDRNCVIna-qaYINSLIKHEDRlaind-------------------------nkslSIAIWFLRCIDEPQltsrfli 76  Picrophilus torridus
Q9HJD1     22 TISGSGHSDg---lSEYASIRKAAFafvesavlgdqaysp----ienaelafivwilaaLDENIITARTIIKArd----- 89  Thermoplasma acido...
NP_987129  16 NLIDEINDSs---nLKLINDRLKLLfwtknpeitdeenkkkyldflneywdtlraygagDLELFPDKINYLTLila---- 88  Methanococcus mari...
NP_111076  13 EAVKRIANSvrgtkDDDAEIKDHSMrfirnaisg-----------------dqqkeilnVDMLRYIAWVLVALn------ 69  Thermoplasma volca...
Feature 1                                                                     ##  #   #       
2DLA_B     78 -yGIELELVKKANLIIYSERlkrkkeikpeeisidvsta-iefptedvrkiervygkipEYTMKISDFLDLVPDekl--- 152 Pyrococcus horikoshii
1ZT2_D     79 -dVKLIRRYAYAEAKQFRSLlhteneenlleisklldlkinrcdpikfylekkrriiqkEFCVHFIDYLKYTKDlkedw- 156 Sulfolobus solfata...
Q58112    119 -hTPYFNAFVRKYAEIKKIKvik----------------------------------klPNKISVWEFIKIASRsrindl 163 Methanocaldococcus...
O26686    109 kaSREARLVVESAGAMVEDRlnvfggeree-------------------imaevlyelgSEGLRWTELADLLGSgklk-- 167 Methanothermobacte...
YP_447146 106 edSHEAKQVVELEANLIDNRlerlksepnnfqys-----------flkealnqlidtnnVYWNDLKEVIDLGVIdln--- 171 Methanosphaera sta...
Q41UX8     75 -ePGMTSRFAITQRDVFERElnriskedneniif-----------ycdsagieatvdgnTFHIPFDFFIKNNKKisgyky 142 Ferroplasma acidar...
Q6L0D5     77 dqRDIFKNYIMKTARDEDFPdlirnlgi------------------------pvfingnNYKMDFSFFVSYNKRisgyky 132 Picrophilus torridus
Q9HJD1     90 -tVEKQLRLVEHYEDLEEFGsrlgieisv---------------------fgeqigkdhLYRIGVADFATYASRvtgsky 147 Thermoplasma acido...
NP_987129  89 anSKNHNIYIKKLAEKYAEKipi---------------------------------edgDSKINIWDFIDVAANsrnndl 135 Methanococcus mari...
NP_111076  70 -eNIVTARTVIRERDAVEDAlknqqpedienfad------------dlkinmkynrdleRFEIGVFDFVKYASRvtgsqy 136 Thermoplasma volca...
Feature 1          ######                                           
2DLA_B    153 --anyYIYEGRVYLKREd-----------------LIRIWSKAFERNVERGVNM 187 Pyrococcus horikoshii
1ZT2_D    157 klsgqILHKGYVYLDKNq-----------------LIGLIAESIKSKIVEMIRP 193 Sulfolobus solfataricus
Q58112    164 hlerlDLENGFVDITKI------------------KEIFAKEIIRVELMKLGEN 199 Methanocaldococcus jannaschii
O26686    168 -ltdlILKEGRVIIDRDefitsfqdsirdrspdrlYDILVGLEVRETMISRMIM 220 Methanothermobacter thermautotrophicus str. ...
YP_447146 172 ---klLLSNGRVIIEYEdfieeyghliehrnpqtmYEVICGLNLKKNLLKSIIM 222 Methanosphaera stadtmanae DSM 3091
Q41UX8    143 rlvyqKLSRGTIYLSKNq-----------------FIHLLREDFVQKLMDVYSR 179 Ferroplasma acidarmanus Fer1
Q6L0D5    133 rlvyqSLDHGYINLDIEt-----------------FSNIMREYIVSRLLDIYNS 169 Picrophilus torridus
Q9HJD1    148 rlsnqIVREGYVYVDEDv-----------------MAKVLREAFVSFMFDTLDQ 184 Thermoplasma acidophilum
NP_987129 136 hlermILEGDTVLLNQKnqsi----------ynfeKIRLKLKNYVEKVRNAEMP 179 Methanococcus maripaludis S2
NP_111076 137 rlsnqSVIKGIVYCQKDv-----------------AIKILRESFVSNMFKVIDS 173 Thermoplasma volcanium GSS1

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