1WDM,2EJ5,2HW5,2PBP,1UIY,2IEX,1HZD,2VRE,2PPY,2VSS,1RJM,1NZY,1EF8,3BPT,1SG4,2PG8,2A7K,2F6Q,2GTR,1WZ8,1SZO,2FW2,2FBM,2Q35,2J5G,1HNO


Conserved Protein Domain Family
crotonase-like

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cd06558: crotonase-like 
Click on image for an interactive view with Cn3D
Crotonase/Enoyl-Coenzyme A (CoA) hydratase superfamily. This superfamily contains a diverse set of enzymes including enoyl-CoA hydratase, napthoate synthase, methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA dehydratase, and dienoyl-CoA isomerase. Many of these play important roles in fatty acid metabolism. In addition to a conserved structural core and the formation of trimers (or dimers of trimers), a common feature in this superfamily is the stabilization of an enolate anion intermediate derived from an acyl-CoA substrate. This is accomplished by two conserved backbone NH groups in active sites that form an oxyanion hole.
Statistics
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PSSM-Id: 119339
View PSSM: cd06558
Aligned: 696 rows
Threshold Bit Score: 65.6596
Threshold Setting Gi: 23397616
Created: 8-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:substrate binding site [chemical binding site]
Evidence:
  • Structure:2VSS; Pseudomonas hydroxycinnamoyl-CoA hydratase monomer with bound acetyl CoA
    View structure with Cn3D
  • Structure:1NZY, Pseudomonas 4-chlorobenzoyl CoA dehalogenase trimer with bound 4-hydroxybenzoyl-CoA, contacts at 4 A.
    View structure with Cn3D
  • Citation:PMID 8679561
  • Comment:The substrate binding site is located at the interface between two subunits in a trimer. Each trimer contains three substrate binding sites.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                # #                               #   #####             
1WDM_A         8 ITVTAles---giVELKFDLKgesVNKFNRLTLNELRQAVDaikadasvKGVIVSSgKDVFIVGADITefvenfklpda- 83  Pseudomonas fragi
2VSS_E        11 VKVEIed----giAFVILNRPe-kRNAMSPTLNREMIDVLEtleqdpaaGVLVLTGaGEAWTAGMDLKeyfrevdagpei 85  Pseudomonas flu...
1HNO_A        11 ISYRIeg----pfFIIHLINPd-nLNALEGEDYIYLGELLEladrnrdvYFTIIQSsGRFFSSGADFKgiakaqgddtnk 85  baker's yeast
ZP_01016219    5 IIMTQee----gvLRLRIAREt-kKNALTQEMYLTLAEAMEgvpdmpeiGAVLFEGsDEIFTAGNDLSafvtappa---- 75  Parvularcula be...
YP_001262344  16 LRAHAad----gvLTVTFDRAe-kRNAITYAMYRALVRLIDhaaaadgvRALSFAAaGSIFTAGHDVSgfaqgaei---- 86  Sphingomonas wi...
XP_660320      8 ITLDItd----qiGVIKLNRPs-vLNSWNEAMLGEMISAFReldqhertVFTVLTGeGRFFSAGADIRqdipkapen--- 79  Aspergillus nid...
NP_106263     11 LRNHT--------LIVTVSSNd-gRPVLDRRAYESLARTFHeaadndevRVVVLRGlAGCFCLGGDFSefldatkh---- 77  Mesorhizobium l...
XP_757746     19 VALSFahncdnrvAVITLNAPe-kLNALGWADYKCITQCLEwialqpdiLVTIFTGkGRYFSAGANVKdpsrtlpdhvak 97  Ustilago maydis...
XP_957770      7 INVEYkg----rlAIITINNEk-kLNALTQMQYYDLAQRLRevathdevYITLIIGtGRYFSAGADVSisrsnvapsegl 81  Neurospora cras...
XP_001386075   6 ILYEVrg----kvTIITFNIPq-kLNALNGEQYLLLAKLVEradkeedtILTLIQSsGRFFSAGANFAdkslantdaadl 80  Pichia stipitis...
Feature 1                                                     # ###                        ##  # 
1WDM_A        84 ---------------eliagnleanKIFSDFEdl-nVPTVAAINGIALGGGLEMCLa-aDFRVMAds--akIGLPEVKLG 144 Pseudomonas fragi
2VSS_E        86 --------------lqekirreasqWQWKLLRmy-aKPTIAMVNGWCFGGGFSPLVa-cDLAICAde--atFGLSEINYG 147 Pseudomonas flu...
1HNO_A        86 yps--------etskwvsnfvarnvYVTDAFIkh-sKVLICCLNGPAIGLSAALVAl-cDIVYSIndk-vyLLYPFANLG 154 baker's yeast
ZP_01016219   76 -----------------dgskppvwRLLEATArc-pVPVIAAVAGPAIGIGTTLLLh-cDLIVAApe--ayFHTPFVDLA 134 Parvularcula be...
YP_001262344  87 -----------------afdekpsyLFMQRLStf-pKPVVAALNGDAVGIGATMLLh-cDLVYAVpg--ctLVFPFTKMG 145 Sphingomonas wi...
XP_660320     80 -----------------ataaekklFYMRKFSrdhtKILVLALNGPGVGGGAAWFTglaDIILAVsg--ayLQVPFNSLG 140 Aspergillus nid...
NP_106263     78 -----------------qkliaavtDMFRTLAtf-pKPILACVDGDAVGVGCTILFh-cDMVIASne--stFRVPFVDFG 136 Mesorhizobium l...
XP_757746     98 adtntahgkatvadfygaraqagqgRLALALRkh-pKILIAALNGPAVGLSAAILSh-cDLVYAYdd--ffLFTPFMSLA 173 Ustilago maydis...
XP_957770     82 ssqd------aihnhwlrnfvannlNITQAFYth-pKILIVGLNGPVIGLSAALVSf-aDFIYCVps--tfLLTPFSSLG 151 Neurospora cras...
XP_001386075  81 fs----------heywlnrfvarntYLTELFHnh-rKILAAAVNGPVIGLSASLLAl-cDLIYVKeekdfyILTPFANLG 148 Pichia stipitis...
Feature 1                                                                           
1WDM_A       145 iypgfGGTVRLPRligvDNAVEWIAsGKENRAEDALKVSAVDAVVTa------dKLGAAALDLIKRA 205 Pseudomonas fragi
2VSS_E       148 ippgnLVSKAMADtvghRQSLYYIMtGKTFGGQKAAEMGLVNESVPl------aQLREVTIELARNL 208 Pseudomonas fluorescens
1HNO_A       155 litegGTTVSLPLkfgtNTTYECLMfNKPFKYDIMCENGFISKNFNmpss-naeAFNAKVLEELREK 220 baker's yeast
ZP_01016219  135 avpegGASYLMPKrlgrQLASEFLLlSRPVPAQRAYEMGFVNAVVTe------gPVRDYGFDLAKDL 195 Parvularcula bermudensis HTC...
YP_001262344 146 lipefGSTFHLPQlighRRTMALFLgSGRCSAEQAVQWGIVNELAAp------dRLAAAAAGALADI 206 Sphingomonas wittichii RW1
XP_660320    141 lvpefGAAQTFAQsigvRRANDLLIfGRKCSVEELENWGLINRVFPa------qGFVDQVLAYLQGQ 201 Aspergillus nidulans FGSC A4
NP_106263    137 lvpdaATSILAPQklgyAGAFRFFClGDTLHAEDARALGLVAEIVHdgv---eeATLGRARQLAKKP 200 Mesorhizobium loti MAFF303099
XP_757746    174 lvaegLTSVTFIKkmglGRATEALLeGRRMTASDLEQSGFITRLYSkp-----aGFHAKDKLATPPI 235 Ustilago maydis 521
XP_957770    152 lvtegGASRGLVQrlglPKANEALImSKKITKEELVQTGFVTKCFEevgkgeteKFKGLVLKEIEER 218 Neurospora crassa OR74A
XP_001386075 149 lvaegAASATLFLrlgwSKASEALLlAKPISGKDLNNLGFINKTYDgqfk-tteEFNQAVHDELVNA 214 Pichia stipitis CBS 6054

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