Conserved Protein Domain Family
GH85_ENGase

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cd06547: GH85_ENGase 
Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
Statistics
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PSSM-Id: 119364
View PSSM: cd06547
Aligned: 31 rows
Threshold Bit Score: 327.332
Threshold Setting Gi: 169865607
Created: 4-Aug-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative active
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 #                           #                                           
AAM80487      126 LLCHDMMGGYLDDRfiqg-svvQTPYAFYHWQCIDVFVYFSHHTvt----iPPVGWTNTAHRHGVCVLGTFITEwn---e 197  human
NP_561734      97 IMNVALTNPYVDRApsqg-sdsMDAYVFSYWQYVNSYVYWGGSSrgi-falPTPDVVDNAHKNGVPVLATIGFPwgpgeg 174  Clostridium p...
NP_784014     104 VVALSIMNSSTSGNaprg-intFDANVFSNWQYIDQLVYWGGSAgeglivpPTADVIDSAHKNGVPVLGTVFFPqvvsgg 182  Lactobacillus...
NP_696499     114 VMAISIMNSSTSGNaphg-lntANANTFSYWQYVDELVYWGGSSgegiivpPSPDVTDMGHTNGVPVLGTVFFPqnvsgg 192  Bifidobacteri...
AAD10851       79 LVSLSALNRHTSGVpsqg-apvFYENTFSYWHYTDLMVYWAGSAgegiivpPSADVIDASHRNGVPILGNVFFPptvygg 157  Arthrobacter ...
ZP_03038066    93 VLSIASMYPSTSGApsqg-sekFHTYTFSNWQYIDKLVMWGGSAgeglivpPSSDVIDAAHKNGVPVFGTVFLPqtehgg 171  Geobacillus s...
NP_241651      78 LVALSALNPNTSGVpsqg-gneFFANTFGFWQYVDLMVYWAGSAgegiitpPSGDVIDAAHRNGVPILGNVFFPpkvygg 156  Bacillus halo...
NP_561205     112 VVAISIMNANTSGNpsqg-snkFSANTFSYWQYIDKLVYWGGSAgeglivpPSPDVTDSAHRNGVPVLGTVFFPmtahgg 190  Clostridium p...
YP_001306262   67 IMVLSIMNPSTSGMpsqgsnnsFKTYPFSFWQYVDYLVAWAGSAgegiivpPSADVIDLAHKNGVYVLGTIFFPpniygg 146  Thermosipho m...
NP_267648     107 IVALSMMNSQTSGNpsrg-ttkFESYTFDYWQYIDTLVYWGGSSgegiivtPSADVIDEAHSNGVPVLGTIFLPpkeygg 185  Lactococcus l...
Feature 1                                            # # #                                        
AAM80487      198 gGRLCEAFLAgdeRSYQAVADRLVQITQFFRFDGWLINIENSl----------------------sLAAVGNMPPFLRYL 255  human
NP_561734     175 yVEQVRAFLQkdnNGNFPVADKMIEIAEYYGFDGYFFNQESYgc---------------------vKDDADRMVEMLEYI 233  Clostridium p...
NP_784014     183 kLKWLNDFLKqdaDGHFPMADKLVAVAKAYGFDGWFINQETDpevksfdsastgktatsstsnglsKKHAQLMQQLIKEY 262  Lactobacillus...
NP_696499     193 kVEWLDQTLAqksDGSFPVADKLIEVAATYGFDGWFINQETEgene----------------tslgADYATKMQAFIAYL 256  Bifidobacteri...
AAD10851      158 qLEWLEQMLEqeeDGSFPLADKLLEVADYYGFDGWFINQETEga---------------------dEGTAEAMQAFLVYL 216  Arthrobacter ...
ZP_03038066   172 kIQWLHDLLKqreDGSFPVADKLIEVATYYGFDGWFINQETQgg---------------------tPEDAAKMAEFLTYL 230  Geobacillus s...
NP_241651     157 qEEWVDKMLVrgeDGSFPAADKLLEVAEYYGFDGWFINQETEgg---------------------tPETAKDMQEFLLYL 215  Bacillus halo...
NP_561205     191 kMEWLNKFLEkdaNGNFPIVDKLIEVAENYGFDGWFINQETEgteq----------------epltPEHAKLMQELIKQF 254  Clostridium p...
YP_001306262  147 rRNWVDQLLKe-eNGKFIIADKLIEVAETYGFDGWFINQETKgc---------------------nETHVKKMINFLNYL 204  Thermosipho m...
NP_267648     186 kVDWVKTMLKkdeQGQYPFASQMVKVAKTYGFEGWFINEETQgl---------------------nADDAANMKALIQQV 244  Lactococcus l...
Feature 1                                                             # ##                        
AAM80487      256 TTQLhrqvpgGLVLWYDSVVqsGQLKWQDELNQHNRVFF------DSCDGFFTNYNWREEHLerm--------------l 315  human
NP_561734     234 KKKAp----nMVIGWYDSMTvdGNVKWQDALNDKNAAFFqng-enRTTDEFFLNYNWTPEKIettv-----------nta 297  Clostridium p...
NP_784014     263 KQKAgs---kLDLMWYDSMTkdGKMDWQNALTKENQSYLvdanmkPVADSMFLNFWWTKKRLasqellk-----kshera 334  Lactobacillus...
NP_696499     257 KKQAp----dLRVVYYDSMTkdGSIDWQNALTDENSMYMtdg-dhPIADEMFLNFWWTEDKLagddlla-----asatka 326  Bifidobacteri...
AAD10851      217 QEQKpe---gMHIMWYDSMIdtGAIAWQNHLTDRNKMYLqng-stRVADSMFLNFWWRDQRQsne-------------la 279  Arthrobacter ...
ZP_03038066   231 QQNQap---gMEVIWYDSMIeeGPVKWQGALTDKNEMFFqqg-nqRVSDNMFLDFRWQFKDEkngqydyitpflnspaka 306  Geobacillus s...
NP_241651     216 QENKpe---gMHIMWYDSMIsnGDIRWQNYLTDENAMFFqag-nrKVADSMFLNFWWWNHSQersk-----------qka 280  Bacillus halo...
NP_561205     255 KAKSng---kFEIMWYDSMTkdGDMDWQNALTDKNEYFLldgdknKVADSIFLNFWWTYNSLkdkdllr-----vsneka 326  Clostridium p...
YP_001306262  205 KEKAp----wMKVVWYDAMSkiGAIEWQGELNENNVVYIvnn-dkKLVDAIFLDFRWQSLKRpetien-------tlntm 272  Thermosipho m...
NP_267648     245 KKEDs----sLQIMWYDAMTkdGKVDWQNQLNDQNATFVq----dKAADAMFLNFWWTQNNLadqklle-----ksnlya 311  Lactococcus l...
Feature 1                                                                                         
AAM80487      316 gQAGERRADVYVGVDVFARGNVVggrfdtdkslELIRKHGFSVALFAPGWVYEClekkdf-------------------- 375  human
NP_561734     298 kSLGRSPFDVYAGLDVQQNAYNTpfnd--dyllDENGKLRLSLAMYTPNSTFSMakdvydfykhdqkfw----------- 364  Clostridium p...
NP_784014     335 eKLGISPYNLFAGIDVQADGTATpvrw--nlfaNKQHVPYTSLGLYAPDWTHASsdtvdefqakegalwv---------- 402  Lactobacillus...
NP_696499     327 kELGIDPYSLYAGIDVQADGYDTpvkw--nlfaGKDGKTHTSLGLYCPSWAYWSagnpttfrknesrlwv---------- 394  Bifidobacteri...
AAD10851      280 qALGRSPYDLYAGVDVEARGTSTpvqw--eglfPEGEKAHTSLGLYRPDWAFQSsetmeafyekelqfw----------- 346  Arthrobacter ...
ZP_03038066   307 aELGRSPYDLYAGIDVEAKGYEGrfnw--pvlfPDGKKATTSLGIYRPDWAYNSsetheeymnkeqifwvg--------p 376  Geobacillus s...
NP_241651     281 aSLGRSPYDLYAGIDVEANGTNTyvnw--qqvfPEGGEPHTSLGIYRPDWAFKStdtmrdfydreqlfw----------- 347  Bacillus halo...
NP_561205     327 kEIGINPYDLYAGIDVQANGYKTplrw--thyqRDDQAPFTSLGLYCPSWTYFDaktpeqfqenenrlwv---------- 394  Clostridium p...
YP_001306262  273 kKYNLKKDDIFVGFDLQANGFYTysnw--pkiiDENGKLKVSLGFYCPSWAYYStkniskfwqneetlwiadhpvltyng 350  Thermosipho m...
NP_267648     312 kNHNIDPYNIYAGIDVQAKDVQTpvkw--nlleKGNQATQTSIGLYAASATYTNasnwddfqnresafwv---------- 379  Lactococcus l...
Feature 1                                                                                         
AAM80487      376 ----------fqnqdkFWGRLERYLPTHSic-sLPFVTSFCLGMGARRVCYGqeeAVGPWYHLSAQEIQPLFGEHRLGGD 444  human
NP_561734     365 --vgptgdpsksdteqDWTGLANYVPDRSaindLPFVTNFNLGHGEDYYIDGslsRDEEWNNRAMQEYLPTWRWIVESNG 442  Clostridium p...
NP_784014     403 ndqndpsrsipsttstHWPGVSTYAVEQSaitkQPFVTNFSLGNGYNYFIKGknvSLRDWNNRSLQAVNPTYRWLINNGN 482  Lactobacillus...
NP_696499     395 ndegnpsvstpyeddeKWTGVSNYVAEQSavtsLPFVTNFNNGSGYSFFREGkqiSKMDWNNRSVSDIQPTYRWIVADEG 474  Bifidobacteri...
AAD10851      347 --vgstgnpaetdgqsNWPGMAHWFPAKStatsVPFVTHFNTGSGAQFSAEGktvSEQEWNNRSLQDVLPTWRWIQHGGD 424  Arthrobacter ...
ZP_03038066   377 gmnpantsqpegtdplAWRGIAHDVVAKTvltdSEFVTHFNTGNGHLFAVDGkvmRSRDWSNRSLQDILPTWRWITETNG 456  Geobacillus s...
NP_241651     348 --vgpsgnpakvdehaEWKGMATYFPEKTvineLPFVTHFNTGSGQFFAINGktkSDVSWNNRSLQDLLPTWRWMAESGG 425  Bacillus halo...
NP_561205     395 -nehgdpskttnaqgvDWRGISTYSVEKSavtsLPFTTNFNIGNGYDFYVDGnkvSTQDWNNRSLNDIMPTYRWVISNEE 473  Clostridium p...
YP_001306262  351 keeffkikwnenykqyRWQAPSKFVVEKSpitsLPFITHFNAGHGYAFYVDGkklSNIPWNNRSLMDIFPTYRWKVTGPI 430  Thermosipho m...
NP_267648     380 -nqkadprqvdhsvneSWTGLSKYVLEKSaisgNEFNTNFNLGNGYNYFKAGqkiSEMDWNDRSLAGILPSYRWIIDNEG 458  Lactococcus l...

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