2NW0


Conserved Protein Domain Family
GH25_PlyB-like

?
cd06523: GH25_PlyB-like 
Click on image for an interactive view with Cn3D
PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus anthracis. PlyB has an N-terminal glycosyl hydrolase family 25 (GH25) catalytic domain and a C-terminal bacterial SH3-like domain, SH3b. Both domains are required for effective catalytic activity. Endolysins are produced by bacteriophages at the end of their life cycle and participate in lysing the bacterial cell in order to release the newly formed progeny. Endolysins (also referred to as endo-N-acetylmuramidases or peptidoglycan hydrolases) degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
Statistics
?
PSSM-Id: 119383
View PSSM: cd06523
Aligned: 10 rows
Threshold Bit Score: 210.296
Threshold Setting Gi: 116334530
Created: 25-Jul-2008
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1           #                         #                                       # #        
2NW0_A         2 YIVDMSKWngspdw---dtakgQLDLVIARVQDGSny-----------vDPVYKDYVAAMKARNIPFGSYAFCRFvsvED 67  unidentified phage
NP_832034      3 YIVDISKWngtinw---diaasQLDLVIARVQDGSnt-----------vDFMYQNYVSEMKKHSIPFGNYAFCRFisiAD 68  Bacillus cereus...
AAM99642      67 PIIDVSGWqlpkeid-ydtlskNISGVVIRVFGGSkisktnnaayttgiDKSFKTHIKEFQKRNIPVAVYSYALGssvKE 145 Streptococcus a...
NP_267296     63 PVVDLSGWqrpedid-yntlsqHVIGAVIRVNGSYghadns--askdgeDTAYKQHIKAFQERGIPTAVYAFVTGentSE 139 Lactococcus lac...
YP_001513967   1 MIIDISSHqvpanmn-ydelakQVKLVIIRTQYGSrl-----------iDKHYKTHHREFKKRGIPTAAYAWVRGtsiED 68  Alkaliphilus or...
NP_784439    429 AIVDVSEFqsdinw--sqvrnaGLALGVIRIQDGEny-----------iDVKHVKNLQGVLANNLNYAVYAFFRGvneAD 495 Lactobacillus p...
YP_535690     45 KIIDVSEWqgvidw--nkviadDVTLSIIRVQHGSah-----------qDLKYMENLQQCISAGGKYAVYAYFAAtstSD 111 Lactobacillus s...
NP_785336     29 AVPDISEWqgqltstevqslksQADFVINRVQYGSsy-----------eDLYHTSNESLYVKYGVPFGSYDYATFtstAT 97  Lactobacillus p...
YP_254544     77 RVLDISEWqgnltdsqvkqlkkDYDFIIIRGQYGSey-----------vDQCLENNSALLDKNNMKFGVYSYSLYenaDD 145 Staphylococcus ...
YP_796057     36 KIADISQYqgninw---skaskDLKYAIIRVQHGSkgda------gymvDSKRNVNANGAYKYGVPFGQYMFAEFtsvKD 106 Lactobacillus b...
Feature 1                                   # #                                  #               
2NW0_A        68 AKVEARDFWNRgdk------dslFWVADVEvttm----sdmRAGTQAFIDELYRLga--kKVGLYVGHHKyeefga-aqi 134 unidentified phage
NP_832034     69 AKKEAQDFWNRgdq------sakFWVADVEvqtm----admQGGIQAFIDELRRLga--eKVGLYVGHHTylsfga-cni 135 Bacillus cereus...
AAM99642     146 MKEEAQIFYKNaapy-----kptFYWIDVEeetm----snmNKGVQAFRKELKRLga--kNVGIYIGTYFmteqgi-svk 213 Streptococcus a...
NP_267296    140 MRKQARDFYRRaspy-----kptYYWLDVEvtnm----knmNQGIEAFRSELEKQga--kNIGIYAQDWFlrdnqi-kvd 207 Lactococcus lac...
YP_001513967  69 MRVEATDFYNRtkef-----nptIWFIDVEeqsm----ddmSSGVSAYVKTLRDLga--kKVGIYIGHHLyktfnl-nle 136 Alkaliphilus or...
NP_784439    496 SQAEARAFYQRvqnvvkgqqqprFYALDIEaien----nnmRGTVNAYISQLNDLgilnsQIVLYIANHLyqqlnl-dtt 570 Lactobacillus p...
YP_535690    112 AQQEARDFYNRtqkvvagkqqpiFYAIDVEsiemsgdvtqmRAGVEAYMSQLNTLgvpdnKIVLYIANHLydkfnl-nva 190 Lactobacillus s...
NP_785336     98 AKAEADKFYQRank------ntkFYVLDFEttsms--staaNAAVKAWYTEMRSLts--kKLIFYSYQSFattyantarq 167 Lactobacillus p...
YP_254544    146 ARNEAQTLYSRapk-------asFYVNDFEqnsit--sgdaDTATKAWADEMKQLsg-nkKVLFYSYENFmtnnvpnavs 215 Staphylococcus ...
YP_796057    107 AQQEAKDFYKRsna------htkFFVLDEEkrmss-aksseQTYVNAWLKTMRSLtd--kPLIMYAGEYYanahnl-dfs 176 Lactobacillus b...
Feature 1             #                        #              #    
2NW0_A       135 kcDFTWIPRYGakp---------aypCDLWQYDEYGQVPGig-kCDLNRL 174 unidentified phage
NP_832034    136 eaDFVWIPRYGgnkp--------aypCDMWQYADSGNVPGig-kCDLNRL 176 Bacillus cereus ATCC 14579
AAM99642     214 gfDAVWIPTYGsdsgyyeaapqtelkYDLHQYTSQGYLPGfnqpLDLNQI 263 Streptococcus agalactiae 2603V/R
NP_267296    208 kfKAIWIAAYGrntgywdaspettlsYKMQQFTDQGTLPGysgnVDLNMV 257 Lactococcus lactis subsp. lactis Il1403
YP_001513967 137 evDAVWIPHYGrndgtln--stpiypCDIHQYTDKGKLNGysgyLDLNRL 184 Alkaliphilus oremlandii OhILAs
NP_784439    571 kvGSIWIPSYGtkp---------rypYDLWQYTDKGTLAGiptkVDMSQD 611 Lactobacillus plantarum WCFS1
YP_535690    191 rpGAIWIPSYGqndgtlanslkpthpYDLHQYTSKGSVNGitgnVDMSAE 240 Lactobacillus salivarius UCC118
NP_785336    168 sfDAQWIANYSskp---------tisFSLWQYSSSYYLSSlgqyVDNSLY 208 Lactobacillus plantarum WCFS1
YP_254544    216 ayDGYWLAAYQeqep--------nreKVLWQYTDSYYAPElnqnVDANYI 257 Staphylococcus haemolyticus JCSC1435
YP_796057    177 kfDGSWIAKYSstkps-----vsgtsIDLWQYTSSGSVSGisgsVDLNKT 221 Lactobacillus brevis ATCC 367

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap