Conserved Protein Domain Family
GT_MraY-like

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cd06499: GT_MraY-like 
Glycosyltransferase 4 (GT4) includes both eukaryotic and prokaryotic UDP-D-N-acetylhexosamine:polyprenol phosphate D-N-acetylhexosamine-1-phosphate transferases. They catalyze the transfer of a D-N-acetylhexosamine 1-phosphate to a membrane-bound polyprenol phosphate, which is the initiation step of protein N-glycosylation in eukaryotes and peptidoglycan biosynthesis in bacteria. One member, D-N-acetylhexosamine 1-phosphate transferase (GPT) is a eukaryotic enzyme, which is specific for UDP-GlcNAc as donor substrate and dolichol-phosphate as the membrane bound acceptor. The bacterial members MraY, WecA, and WbpL/WbcO utilize undecaprenol phosphate as the acceptor substrate, but use different UDP-sugar donor substrates. MraY-type transferases are highly specific for UDP-N-acetylmuramate-pentapeptide, whereas WecA proteins are selective for UDP-N-acetylglucosamine (UDP-GlcNAc). The WbcO/WbpL substrate specificity has not yet been determined, but the structure of their biosynthetic endproducts implies that UDP-N-acetyl-D-fucosamine (UDP-FucNAc) and/or UDPN-acetyl-D-quinosamine (UDP-QuiNAc) are used. The eukaryotic reaction is the first step in the assembly of dolichol-linked oligosaccharide intermediates and is essential for N-glycosylation. The prokaryotic reactions lead to the formation of polyprenol-linked oligosaccharides involved in bacterial cell wall and peptidoglycan assembly. Archaeal and eukaryotic enzymes may use the same substrates and are evolutionarily closer than the bacterial enzyme. Archaea possess the same N-glycosylation pathway as eukaryotes. A glycosyl transferase gene Mv1751 in M. voltae encodes for the enzyme that carries out the first step in the pathway, the attachment of GlcNAc to a dolichol lipid carrier in the membrane. A lethal mutation in the alg7 (GPT) gene in Saccharomyces cerevisiae was successfully complemented with Mv1751, the archaea gene.
Statistics
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PSSM-Id: 133460
View PSSM: cd06499
Aligned: 10 rows
Threshold Bit Score: 142.055
Threshold Setting Gi: 15678761
Created: 8-Jan-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Mg++ bindingputative
Feature 1:Mg++ binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                      ##                
Q6AJ51        70 GVPTMGGLlillsvtvstllwar-----------------ldnpliwlvllvtlFFGMIGAYDDykkiskkt-------- 124 Desulfotalea ps...
XP_001624100  48 PIPEGLGAvggalflicmflfiplpflsiwlekgdfdfphhefvmfiaallsicCMIFLGFADDvldl------------ 115 starlet sea ane...
YP_843372     41 KVPEMGGVplvfgfvlgvlvyigietf---------ylnsyrytpilaalctvlMACIIGIMDDilgwka---------- 101 Methanosaeta th...
ZP_01666735   44 PIPRLGGLaiylgfvlavlasm------------------hvnreimgllmggtVILVVGIIDDlkql------------ 93  Thermosinus car...
YP_001130131  52 PTPRIGGLaifaalfvawlllpk-----------------gsgmllglmliaslPAFVAGFIEDltkr------------ 102 Prosthecochlori...
NP_614037     43 GMPIMGGLgillgtasgplla---------------------siqalppiasalPMMVLGIVDDllglqveefqkvvrnv 101 Methanopyrus ka...
NP_275878     45 GTPTMGGLgmllalllvtvlyr-------------------nnpylvltslivlTAAIVGLLDDllglkvkevqriirnv 105 Methanothermoba...
YP_001272933  51 GTPTMGGIaflfvilliiaiyy-------------------nntpilimsyimlAGGIVGLLDDliglkvkeiqkvvvnc 111 Methanobrevibac...
YP_448238     47 GTPTMGGFgiligvsvmalflfd----------------whstsyllitvimmvVAGIFGMFDDllgfkvkeyqkvvrnd 110 Methanosphaera ...
Q56918        44 PTPRGGGVaivisfligiilfyfl--------------gylpilsvvglivsggVIALVGFWDDhghi------------ 97  Yersinia entero...
Feature 1                                                                                        
Q6AJ51       125 ------------------------------------------------seglsakGKLLLQIAGALIvgffvylhpgydg 156 Desulfotalea ps...
XP_001624100 116 ----------------------------------------------------kwrDKLILPTMASLPllmvyfvnigvtt 143 starlet sea ane...
YP_843372    102 --------------------------------------------------glrqwQKPMFMLFAAMPmmvinaghttmsl 131 Methanosaeta th...
ZP_01666735   94 ----------------------------------------------------parVKLLGQIVAAMVlllfnvriewltn 121 Thermosinus car...
YP_001130131 103 ---------------------------------------------------vgvrERLLATILSGVAayyltgysltrvd 131 Prosthecochlori...
NP_614037    102 snrpielgrlilqpgeearvatekakrdlkrileedpdaieivgevpikkelserEKLALQTGAALFvlpivpatwlwip 181 Methanopyrus ka...
NP_275878    106 segplevgqlvlkpgeear--aatdkakrdveallseglvevvgeapiknevsegEKILAQLLIGVFlvlsgavgklgg- 182 Methanothermoba...
YP_001272933 112 cdevitlgrldvepneevr--vatpkakkeidklveegkveiigevpiktepgelEKIVFQILLGVLlgltgvittigg- 188 Methanobrevibac...
YP_448238    111 gdkpvsiglltlepgeear-iasekakadynkiveqehkltlideipiksettesEKIITQFVVACFvilpgflptsvs- 188 Methanosphaera ...
Q56918        98 ----------------------------------------------------aarWRLLAHFSAAAFllfcfggfpvlnv 125 Yersinia entero...
Feature 1                                                                                        
Q6AJ51       157 qlsip---fmknvqpdlgwfyivfaVIVIVGASNAVNLTDGLDGLAAGPMVVSSAvyllfaylagnvvlanylhipyvag 233 Desulfotalea ps...
XP_001624100 144 iivpkpvrfifgfdldlgilyyvymGMLAVFCTNAINILAGINGVEAGQSLIIAMsivlfnllelqgtccwq-------n 216 starlet sea ane...
YP_843372    132 pl--------igrvdwgilyplviiPIGVVGASNAFNMVAGYNGLEAGMGVIIFAalgyaglvmgkts------------ 191 Methanosaeta th...
ZP_01666735  122 pf---------gdmiyldyfsipltILWVVGLTNTVNLIDGLDGLAAGVSTIASItillvalqqnfwt------------ 180 Thermosinus car...
YP_001130131 132 vpg-------fdvllqwmpftvaftAFAVGGVANSVNIIDGFNGLAGGVLMICFGmlaaiawqvgdth------------ 192 Prosthecochlori...
NP_614037    182 k----------vgvwnlsylfypvaVFGIVGATNAVNLIDGMDGLAAGLLFLASLacsgvclgqgqlp------------ 239 Methanopyrus ka...
NP_275878    183 --------------fylglaaapiaIAGMVGAINAVNLIDGMDGMAAGIMLIASLscaiflglsgqa------------- 235 Methanothermoba...
YP_001272933 189 --------------fnlgilafpvvIIAILGCINCVNLIDGMDGLAAGIVGIASFacccylylcgnmg------------ 242 Methanobrevibac...
YP_448238    189 -------------gfelgilmiplaLFAIIGSINSVNLIDGMDGLAAGIIAIASIasalyanvttgsv------------ 243 Methanosphaera ...
Q56918       126 s----------gfiielgifgslfgLLFLVWMLNLYNFMDGIDGLASAEAVTACIgmiaiyyisgdhiel---------- 185 Yersinia entero...
Feature 1                                                      
Q6AJ51       234 sgelaifcgtlfgaclGFLWFNAHPAqmFMGDVGSLALGGALGSIA 279 Desulfotalea psychrophila
XP_001624100 217 hlfslyfmlpfiavcsALLYYNWYPSsiFVGDTFCYFAGMAFAVVG 262 starlet sea anemone
YP_843372    192 ---aaalsiimlgsllAFLYFNRYPArvFPGDTMTYSVGALAACVA 234 Methanosaeta thermophila PT
ZP_01666735  181 ---vailtaalagsalGFLYHNFNPAkiFMGDTGSMFLGYMLAAVS 223 Thermosinus carboxydivorans Nor1
YP_001130131 193 ---laklcilfilcvaGFMFLNFPFGkiFMGDGGAYLMGFLLAWTA 235 Prosthecochloris vibrioformis DSM 265
NP_614037    240 ---gaaffaslagaslGFLFHNRYPAkiFMGDTGSCFLGAAYAAGA 282 Methanopyrus kandleri AV19
NP_275878    236 -----lpflalagmcaGFLVFNRHPAsiFMGDTGSFALGAGYATAV 276 Methanothermobacter thermautotrophicus str. Delta H
YP_001272933 243 ---gmpvfiiltglclGFLVFNKYPAsiFMGDTGSFVLGTGYAAAV 285 Methanobrevibacter smithii ATCC 35061
YP_448238    244 ---aalpfiiiagacvGFLVLNHYPAkiFMGDTGSYALGTGYMTAA 286 Methanosphaera stadtmanae DSM 3091
Q56918       186 --nsflvlwllactvlGFLLWNFPPAkiFMGDAGSGFLGLMIGSLA 229 Yersinia enterocolitica

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