2H50,2H50,1GME,1GME,2BYU


Conserved Protein Domain Family
ACD_ScHsp26_like

?
cd06472: ACD_ScHsp26_like 
Click on image for an interactive view with Cn3D
Alpha crystallin domain (ACD) found in Saccharomyces cerevisiae (Sc) small heat shock protein (Hsp)26 and similar proteins. sHsps are molecular chaperones that suppress protein aggregation and protect against cell stress, and are generally active as large oligomers consisting of multiple subunits. ScHsp26 is temperature-regulated, it switches from an inactive to a chaperone-active form upon elevation in temperature. It associates into large 24-mers storage forms which upon heat shock disassociate into dimers. These dimers initiate the interaction with non-native substrate proteins and re-assemble into large globular assemblies having one monomer of substrate bound per dimer. This group also contains Arabidopsis thaliana (Ath) Hsp15.7, a peroxisomal matrix protein which can complement the morphological phenotype of S. cerevisiae mutants deficient in Hsps26. AthHsp15.7 is minimally expressed under normal conditions and is strongly induced by heat and oxidative stress. Also belonging to this group is wheat HSP16.9 which differs in quaternary structure from the shell-type particles of ScHsp26, it assembles as a dodecameric double disc, with each disc organized as a trimer of dimers.
Statistics
?
PSSM-Id: 107229
View PSSM: cd06472
Aligned: 19 rows
Threshold Bit Score: 125.495
Threshold Setting Gi: 115464729
Created: 18-Mar-2008
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
dimer interface
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:
  • Structure:2H50, Saccharomyces cerevisiae Hsp26 dimer interface, contacts at 3.5 A
    View structure with Cn3D
  • Comment:ScHsp26 assembles in shell-like particles composed of 24 subunits. These particles are storage forms which upon heat shock disassociate into dimers. Non-native substrate proteins bind the disassociated ScHsp26 and assemble into large globular assemblies having one monomer of substrate bound per dimer.
  • Structure:1GME_A, Triticum aestivum sHsp dimer interface, contacts at 3.5 A
    View structure with Cn3D
  • Comment:T. aestivum HSP16.9 assembles as a 12-mer, comprised of two discs, each disc having 6 alpha crystallin-like domains organized as a trimer of dimers.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1        #######      # # ##                      #    #      #########      #    #      
2H50_A         2 RMDWKETPEAHVFKADLPGVKKEEVKVEVEDg-nVLVVSGERTKEKEDk----NDKWHRVERs-----SGKFVRRFRLLE 71  baker's yeast
123562        76 RVDWKETPEGHVIMVDVPGLKKDDIKIEVEEn-rVLRVSGERKKEEDKk----GDHWHRVERs-----YGKFWRQFKLPQ 145 pea
XP_001770731  53 SVDWKETATEHVIKADVPGLSKNEIKVEVDDtqrVLRINGERRKEEERq----TDEWHVLERg-----DARYLRQLALPE 123 Physcomitrella ...
ABK26632      27 QVDWLETPNAHIFKVNVPGMNKDDIKIQVEDg-hILHIKGEGKKEEDKt----EGMWHCMERg-----RGSFSRQFGLPE 96  Sitka spruce
NP_198583     23 LIDWMESNNSHIFKINVPGYNKEDIKVQIEEg-nVLSIRGEGIKEEKKe----NLVWHVAEReafsggGSEFLRRIELPE 97  thale cress
CAH67235      77 RVDWRETGDAHEVVVDVPGMRKEDLRVEVEDn-rVLRISGERRREETTeqkggGDHWHREERs-----YGRFWRQLRLPD 150 Indian rice
XP_001783868   2 RMDWRETADAHILKTDMPGVRSDDVKVQVIDg-eVVEISGTRKKEEPKe----GDEWHHVERp-----SGFFFRSFRIPE 71  Physcomitrella ...
ABQ96645      45 KIYMKETAEAHVIKMEVPGLKREEVKVELEEg-nTVKICGEKIVEREEr----NGYWYRVERs-----GGRFVRSIRLPE 114 Viola baoshanensis
ABK26869      79 HVDWWESSDAHIIQADLPGATKDDVEIIVENg-rVLQISGRSKMAVPPg----GGRCRRGERs-----RVGYLRRLRLPS 148 Sitka spruce
NP_001055964  94 TVDWAETDSEYYLRADIPGGRKCDVEVSGDDamrVVDVSGLWRAAPPPpp-pdGRDWRAGRWw-----EHGFVRRVELPE 167 Japanese rice
Feature 1                    #  ##       
2H50_A        72 DAKVEEVKAGLEN--GVLTVTVPK 93  baker's yeast
123562       146 NVDLDSVKAKMEN--GVLTLTLHK 167 pea
XP_001770731 124 NANLDQITASVDN--GVLTVTMPK 145 Physcomitrella patens subsp. patens
ABK26632      97 DVKMDHIKAQVEN--GVLTIIAPK 118 Sitka spruce
NP_198583     98 NVKVDQVKAYVEN--GVLTVVVPK 119 thale cress
CAH67235     151 NADLDSIAASLDN--GVLTVRFRK 172 Indian rice
XP_001783868  72 NAKADDLKAQVAD--GVLTITLPK 93  Physcomitrella patens subsp. patens
ABQ96645     115 NANGQEMKACLDN--GVLFITVPK 136 Viola baoshanensis
ABK26869     149 NADAEQLKAEMEN--GVLTVTIPK 170 Sitka spruce
NP_001055964 168 DADWRKVEAFFDDgeGLLEIKVPK 191 Japanese rice

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap