Conserved Protein Domain Family
ACD_IbpA-B_like

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cd06470: ACD_IbpA-B_like 
Alpha-crystallin domain (ACD) found in Escherichia coli inclusion body-associated proteins IbpA and IbpB, and similar proteins. IbpA and IbpB are 16 kDa small heat shock proteins (sHsps). sHsps are molecular chaperones that suppress protein aggregation and protect against cell stress, and are generally active as large oligomers consisting of multiple subunits. IbpA and IbpB are produced during high-level production of various heterologous proteins, specifically human prorenin, renin and bovine insulin-like growth factor 2 (bIGF-2), and are strongly associated with inclusion bodies containing these heterologous proteins. IbpA and IbpB work as an integrated system to stabilize thermally aggregated proteins in a disaggregation competent state. The chaperone activity of IbpB is also significantly elevated as the temperature increases from normal to heat shock. The high temperature results in the disassociation of 2-3-MDa IbpB oligomers into smaller approximately 600-kDa structures. This elevated activity seen under heat shock conditions is retained for an extended period of time after the temperature is returned to normal. IbpA also forms multimers.
Statistics
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PSSM-Id: 107227
View PSSM: cd06470
Aligned: 35 rows
Threshold Bit Score: 106.848
Threshold Setting Gi: 50470482
Created: 18-Mar-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative dimer
Feature 1:putative dimer interface [polypeptide binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         #####        # # ##                                                #          
P0C058       33 PPYNIEKsddnHYRITLALAGFRQEDLEIQLEgTRLSVKGTpeqpk-------eekkwLHQGLMNQPFSLSFTLAENMEV 105 Escherichia coli...
YP_256990    41 PAYNLLKns-eQHELTVSVPGYAQDELDVSVLnNQLTVRGKpnvektdeaeekegikwLHQGIRKSEFVLSFTLEHRIVI 119 Sodalis glossini...
ZP_01896848  75 PRYNITHvgelDYQITLALAGFSLEEIDITVTaDELVITGKrqpvd-------vtstwLHQGINQADFSRKFALADHVQV 147 Moritella sp. PE36
YP_265605    42 PPYNIRKtgkdNYAIEVALAGFNKNDVEVEFEdNLLTVRTKqvdksed---ksddgeiIHKGISQRQFARSFTIAEDVKV 118 Candidatus Pelag...
YP_054435    18 PNYNLIQldkyNYELSVSVPGYKKEELEVSIInNRLNINSKskkekek---etkkinwIHQGILKNNFSISFKLEHKIKI 94  Wigglesworthia g...
ZP_01018030  36 PPYNIVRtsetDYEITLAVAGFSQDDLEIEVVdRELRVSGRrdsdqd------edreyLHQGIAGRNFDRRFRLAEHLTV 109 Parvularcula ber...
Q9Z616       42 PAYNLFQidehKYELILSIPGYEEKELDISVHnSQLTVQGKkqnqend---dkkikkyLHKGIIFNDFSLNFNFDHKIQV 118 Buchnera aphidic...
O31288       42 PPYDILQlkenFYKLIVSVPGYLEKNLEISTQyDQLIIIGKkeitentnekketvekiLHQEIYTGDFSLSFRLNERISV 121 Buchnera aphidic...
NP_858065    41 PSYDLIQknenNYQLIINTAGYKEEDLDILVHnNKLTVTGKykenin-----nkdnynGCIKGLKKYFSLSFNLDNRIKV 115 Buchnera aphidicola
ZP_02961025  43 QTYDLKQiddsHYELTVSVPGYQESDLTVSLKgGRLLVEGKreeksq-----ednekwLHRGISYGQFTLQFDLGKNVRI 117 Providencia stua...
Feature 1              ##       
P0C058      106 SGATFVNGLLHIDLIR 121 Escherichia coli K12
YP_256990   120 QQANLANGLLTLHFTY 135 Sodalis glossinidius
ZP_01896848 148 LGAEMAQGLLTINLQR 163 Moritella sp. PE36
YP_265605   119 NGAELKDGLLTIACER 134 Candidatus Pelagibacter ubique HTCC1062
YP_054435    95 KYANLKHGILNLSFFY 110 Wigglesworthia glossinidia endosymbiont of Glossina brevipalpis
ZP_01018030 110 RGAQLENGLLTVLLAL 125 Parvularcula bermudensis HTCC2503
Q9Z616      119 KKAELFSGLLKINFEC 134 Buchnera aphidicola (Schizaphis graminum)
O31288      122 MSATLDQGLLTIYFEY 137 Buchnera aphidicola (Thelaxes suberi)
NP_858065   116 KQAKIILGLLTLDFEY 131 Buchnera aphidicola
ZP_02961025 118 EKADLSRGLLTVAIEY 133 Providencia stuartii ATCC 25827

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