1FC4,2JGT,1BS0,2BWO


Conserved Protein Domain Family
KBL_like

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cd06454: KBL_like 
Click on image for an interactive view with Cn3D
KBL_like; this family belongs to the pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). The major groups in this CD corresponds to serine palmitoyltransferase (SPT), 5-aminolevulinate synthase (ALAS), 8-amino-7-oxononanoate synthase (AONS), and 2-amino-3-ketobutyrate CoA ligase (KBL). SPT is responsible for the condensation of L-serine with palmitoyl-CoA to produce 3-ketodihydrospingosine, the reaction of the first step in sphingolipid biosynthesis. ALAS is involved in heme biosynthesis; it catalyzes the synthesis of 5-aminolevulinic acid from glycine and succinyl-coenzyme A. AONS catalyses the decarboxylative condensation of l-alanine and pimeloyl-CoA in the first committed step of biotin biosynthesis. KBL catalyzes the second reaction step of the metabolic degradation pathway for threonine converting 2-amino-3-ketobutyrate, to glycine and acetyl-CoA. The members of this CD are widely found in all three forms of life.
Statistics
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PSSM-Id: 99747
View PSSM: cd06454
Aligned: 35 rows
Threshold Bit Score: 382.679
Threshold Setting Gi: 28900566
Created: 17-Jun-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Structure:2BWO_B; 5-Aminolevulinate Synthase catalytic residue (lys) and cofactor (PLP)
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                      
1FC4_A      45 SHVINFCANNYLGLANh--pDLIAAAKAGXDSHGfgxasvr-----ficgtqdSHKELEQKLAAFLgXEDAILYSSCFDA 117  Escherichia coli
NP_660957   51 RKLLNLSSNDYLGLGAdrelFSSFIAQIRDDRFDdgrfamtssssrlltghhpVCDQLESAIAAAYgSETALVFNSGYHA 130  Chlorobium tepid...
NP_768737   31 RVGIDFASNDYLALGNa--sRIKKAMVAALEAGTpvgaggsr----llrgnceEHERLETEAAQFFgAEAALFFGGGYIA 104  Bradyrhizobium j...
NP_763736   35 QSYINFTSNDYLGIGQl--eYQPQNFLDFIKTYSihlsssr-----lvsgnsvVYQQLEQAISEHFnFEDALIFNSGYDA 107  Staphylococcus e...
NP_842296   37 RDFLAFCSNDYLGLANh--pALIEAAAEGARCYGvgsgash-----lisghfrAHHELEEALAAFVgLPRTLLFSTGYMA 109  Nitrosomonas eur...
2JGT_A      65 KDTILLGTYNYMGMTFd--pDVIAAGKEALEKFGsgtngsr-----mlngtfhDHMEVEQALRDFYgTTGAIVFSTGYMA 137  Sphingomonas pau...
NP_821275  886 RELLSFSSYNYLGMATh--pQVNEAARKAIERCGtsvsasr-----llsgsrpLHLELEAELAATLgCEAAITLVNGHAT 958  Streptomyces ave...
1BS0_A      39 RQYLNFSSNDYLGLSHh--pQIIRAWQQGAEQFGigsggsg-----hvsgysvVHQALEEELAEWLgYSRALLFISGFAA 111  Escherichia coli...
NP_603746   31 KSLYNFSSNDYLSLAHdkdlLQKFYQNYNFDNYKlsssssr-----lidgsylTVMRLEKKVEEIY-GKPCLVFNSGFDA 104  Fusobacterium nu...
2BWO_B      46 QDITVWCGNDYLGMGQh--pVVLAAMHEALEAVGagsggtr-----nisgttaYHRRLEAEIAGLHqKEAALVFSSAYNA 118  Rhodobacter caps...
Feature 1                                                                                      
1FC4_A     118 NGGLFETLl--gAEDAIISDALNHASIIDGVRlckAKRYRYANndxqeLEARLkeare-agarHVLIATDGVFSXDGVIA 194  Escherichia coli
NP_660957  131 NTGILPALa--tRHDLILSDRLNHASIIDGLRiadADYRRFRHadydhLEEQLetaar-ecyrQIFIVTESVFSMDGDLA 207  Chlorobium tepid...
NP_768737  105 NFALLTTLp--qRGDLLVLDSLVHASIHEGARagrAECRIHAHndpqsVEDAIrawrnkggagRVWIVAESLYSMDGDFA 182  Bradyrhizobium j...
NP_763736  108 NLAVFNIFk--nNNVVIFSDQQNHASIIDGIKlsgLSKVIYQHlnyddLESHLaqdt--hldvQKVIVSDSVFSTNGTKA 183  Staphylococcus e...
NP_842296  110 NMAVVTALa--gRGDAIFADRLNHASLNDAALlsrARFIRYPHldldtLARQLett----karRRLVVTDAVFSMDGDMA 183  Nitrosomonas eur...
2JGT_A     138 NLGIISTLa--gKGEYVILDADSHASIYDGCQqgnAEIVRFRHnsvedLDKRLgrlp---kepAKLVVLEGVYSMLGDIA 212  Sphingomonas pau...
NP_821275  959 NVTVIGHLv--gEGDLIVHDSLAHDSIIQGCRlsgARRRPFPHndaaaLDALLtqvr--hhyrRVLVVAEGVYSMDGDIA 1034 Streptomyces ave...
1BS0_A     112 NQAVIAAMm--aKEDRIAADRLSHASLLEAASlspSQLRRFAHndvthLARLLasp----cpgQQMVVTEGVFSMDGDSA 185  Escherichia coli...
NP_603746  105 NSSVIETFf--dKKSLIITDRLNHASIYEGCInsrAKILRYKHldvsaLEKLLkkys--enynDILVVTETVYSMDGDCA 180  Fusobacterium nu...
2BWO_B     119 NDATLSTLrvlfPGLIIYSDSLNHASMIEGIKrnaGPKRIFRHndvahLRELIaadd---paaPKLIAFESVYSMDGDFG 195  Rhodobacter caps...
Feature 1                                                             #                        
1FC4_A     195 NLKGVCDLADkydALVXVDDSHAVGfvgengrgs---heycdvxgrVDIITGTLGKALGgasGGYTAARkEVVEWLRQRs 271  Escherichia coli
NP_660957  208 DLRRLVKLKRrfkAVLIVDEAHGAGvfgerglgl---cealgvtneIDIIVGTFGKSLAs-aGAYAVMRgLFREYLVNTm 283  Chlorobium tepid...
NP_768737  183 PLEELVAIADrqdAFLIVDEAHATGayghqgrgl----tapydkreNLIVVHTCGKALGa-aGALVTASgILRDFMVNRc 257  Bradyrhizobium j...
NP_763736  184 DINRLVHLKQrynAILIIDASHSLGlnlfe------------yhadIDIVTSSLSKAWGa-hGGIIFSSkDIKDLIINKg 250  Staphylococcus e...
NP_842296  184 PVAELLTLCQrfdAWLLLDDAHGFGvlgergkgslyhsqrierdtpYLIYMATLGKAAGv-sGAFVAAQaPVVETLIQHg 262  Nitrosomonas eur...
2JGT_A     213 PLKEMVAVAKkhgAMVLVDEAHSMGffgpngrgv---yeaqglegqIDFVVGTFSKSVGt-vGGFVVSNhPKFEAVRLAc 288  Sphingomonas pau...
NP_821275 1035 DLPALIEVKRrhgALLMIDEAHSIGvigaagrgig--qyfdvdrqdVELWSGTLSKALAs-cGGYVAAGrTVVDYLRYTv 1111 Streptomyces ave...
1BS0_A     186 PLAEIQQVTQqhnGWLMVDDAHGTGvigeqgrg-----scwlqkvkPELLVVTFGKGFGv-sGAAVLCSsTVADYLLQFa 259  Escherichia coli...
NP_603746  181 EIKQICDLKEkynFNLMVDEAHSYGaygygiay------neklvnkIDFLVIPLGKAGAs-vGAYVICDeIYKNYLINKs 253  Fusobacterium nu...
2BWO_B     196 PIKEICDIAEefgALTYIDEVHAVGmygprgagv---aerdglmhrIDIFNGTLAKAYGv-fGGYIAASaRMVDAVRSYa 271  Rhodobacter caps...
Feature 1                                                                                      
1FC4_A     272 rpylfsnSLAPAIVAASIKVLEXVea--gsELRDRLWANARQFREQXSAagfTLAga--dHAIIPVXLgdaVVAQKFARE 347  Escherichia coli
NP_660957  284 rtlifttALPPMTLAWSLATFTKQlt--mqRERDHLLGLAATLRGSLREagfDVPge--sHIVPVVLGe-dRTAVEMAGA 358  Chlorobium tepid...
NP_768737  258 rpfifatAPSPLMAVAVREALLTLqk--epERQQRLAKLVAFAHRQITSlglRTPst--sQIVPYIVGd-nAHAMRLASA 332  Bradyrhizobium j...
NP_763736  251 rsliyssSLPSYHLYFIQVSLQHVie--dtYRREKLNALSEYFNHQFMElfpNQPls--nTPIKNIVCdslASAQAQYDM 326  Staphylococcus e...
NP_842296  263 rtygyttAAPPLLAHTLLTSLQLIsq--esWRRERLALLIERLRQRLHSlpwPLLls-etPIQPLLVGe-sQEAVRLDLA 338  Nitrosomonas eur...
2JGT_A     289 rpyiftaSLPPSVVATATTSIRKLmt--ahEKRERLWSNARALHGGLKAmgfRLGtetcdSAIVAVMLedqEQAAMMWQA 366  Sphingomonas pau...
NP_821275 1112 pgfvfsaGMTPANTAASLAALRVLra--epQRVARLKENSALFARLAHRagiDTGps-hdTPIIPCIVgdsAKTLQLAEA 1188 Streptomyces ave...
1BS0_A     260 rhliystSMPPAQAQALRASLAVIrsdegdARREKLAALITRFRAGVQDlpfTLAds--cSAIQPLIVgdnSRALQLAEK 337  Escherichia coli...
NP_603746  254 kkfiystALPPVNNLWNLFVLENLvn--fqDRIEKFQELVTFSLNTLKKlnlKTKst--sHIISIIIGd-nLNAVNLSNN 328  Fusobacterium nu...
2BWO_B     272 pgfifstSLPPAIAAGAQASIAFLktaegqKLRDAQQMHAKVLKMRLKAlgmPIIdh--gSHIVPVVIgdpVHTKAVSDM 349  Rhodobacter caps...
Feature 1                                                      
1FC4_A     348 LQke-gIYVTGFFYPVVPkgqARIRTQXSAAHtpEQITRAVEAFTRIG 394  Escherichia coli
NP_660957  359 LRea-gFHALPVRPPTVPensSRLRFSLRADLtsGDIAALAETMKRGA 405  Chlorobium tepidum TLS
NP_768737  333 LQar-gFDIRGIRPPTVPagtARLRISLTLNVgeADVRAMLDALVEVT 379  Bradyrhizobium japonicum USDA 110
NP_763736  327 LFeh-gIFVSYLRYPTVSq--PTLRISLSYFHdtDDIDRLFNVMKQYD 371  Staphylococcus epidermidis ATCC 12228
NP_842296  339 LRer-gIWVPAIRPPTVPqgmARLRISLSAVHteADVDRLGAALRDLA 385  Nitrosomonas europaea ATCC 19718
2JGT_A     367 LLdg-gLYVNMARPPATPagtFLLRCSICAEHtpAQIQTVLGMFQAAG 413  Sphingomonas paucimobilis
NP_821275 1189 LFeq-gISVNPILYPAVPekaARLRFFITCDHtpDQIHHTVRELAHGL 1235 Streptomyces avermitilis MA-4680
1BS0_A     338 LRqq-gCWVTAIRPPTVPagtARLRLTLTAAHemQDIDRLLEVLHGNG 384  Escherichia coli K12
NP_603746  329 LKel-gYLAYAIKEPTVPkdtARLRISLTADMkkEDIETFFKTLKAEM 375  Fusobacterium nucleatum subsp. nucleatum ATCC 25586
2BWO_B     350 LLsdygVYVQPINFPTVPrgtERLRFTPSPVHdlKQIDGLVHAMDLLW 397  Rhodobacter capsulatus

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