2E7J


Conserved Protein Domain Family
SepCysS

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cd06452: SepCysS 
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Sep-tRNA:Cys-tRNA synthase. This family belongs to the pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). Cys-tRNA(Cys) is produced by O-phosphoseryl-tRNA synthetase which ligates O-phosphoserine (Sep) to tRNA(Cys), and Sep-tRNA:Cys-tRNA synthase (SepCysS) converts Sep-tRNA(Cys) to Cys-tRNA(Cys), in methanogenic archaea. SepCysS forms a dimer, each monomer is composed of a large and small domain; the larger, a typical pyridoxal 5'-phosphate (PLP)-dependent-like enzyme fold. In the active site of each monomer, PLP is covalently bound to a conserved Lys residue near the dimer interface.
Statistics
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PSSM-Id: 99745
View PSSM: cd06452
Aligned: 11 rows
Threshold Bit Score: 626.726
Threshold Setting Gi: 11497798
Created: 23-Jun-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                          
2E7J_A           9 FINIDPLQTGGKLTEEARQALLEWGDGYSVCDFCtt-gRLDEIKtPPIHDFIHNQLPKFLgcdVARVTNGAREAKFAVMH 87  Archaeoglobus...
jgi:Mthe_0102   28 EINLDPLQRGGILTPEARAALVEWGDGYSVCDFCe--gMLDRVRnPPVEEFVHEILPRFLdvdDVRITHGAREGMYAAMH 105 Methanosaeta ...
AAB99700        34 FINLNPIQRGGILPKEAKKAVYEYWDGYSVCDYCh--gRLDEVTcPPIKDFLEDIAKFLNm-dCARPTHGAREGKFIVMH 110 Methanocaldoc...
NP_615682       21 SINIDPLQTGGLLTEAARQALVEWGDGYSVCDFCg--gVLDQIKkPPIHDFVHKALPEFLgcdEARVTNGARESKFAVMH 98  Methanosarcin...
jgi:MbarA_1638  21 SINIDPLQTGGRLTEAAKQALVEWGDGYSVCDFCg--gILDQIKkPPIYDFVHKALPEFLdcdEARVTNGARESKFAVMH 98  Methanosarcin...
NP_276195       16 NLNLNPLQRGGVLPAAARKALHEFGDGYSVCDYCd--gRLDQVTrPAINCFLDDLADFTGs-dAVRTVHGAREGKFAVMH 92  Methanothermo...
AAM01446        17 YINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCe--gLLHEIEkPPIRQFHEDLAEFLGm-dVVRITAGARYAKEAVMS 93  Methanopyrus ...
NP_069020       15 MINVHPIQRGGILTEEARKVLLEWGDGYSMCDICle-gRVDLLDkPPVKRFKEEVAEFLGm-dEVRFTAGARHAKFVAMS 92  Archaeoglobus...
YP_565502       83 FINIQPIQAGGRLTPEARKAVIAYGDGYSTCDNCrkpfRLDKIEkPSISTFHTDLAEFVGm-dQARVVPGARRGFQAVAS 161 Methanococcoi...
YP_001047525    16 YINIDPIQAGGRLTVDAMKAAISFADGYSVCDNCrspfRLDYIQkPPIAQFHADLAAWLNm-dTARVVPGARRGFQAVAS 94  Methanoculleu...
YP_001029791    16 SINLDPIQVGGRLTPEAMKAMISWGDGYSVCDNCrkpfRLDYIEkPPLKDFHVDVAEWLGm-aQARTVPGARRGFQQVAG 94  Methanocorpus...
Feature 1                                                                                          
2E7J_A          88 SLAkkdAWVVMDENCHYSSYVAAERAglNIALVPktdypd-yaitpENFAQTIEETkkr--geVVLALITYPDGnyGNLP 164 Archaeoglobus...
jgi:Mthe_0102  106 SLCdagDYIVADGNAHYTTVLAAERAglEISYVEnsgepe-ytinpEGYREAIRDAadq-gyrVSMAVLTYPDGnyGNLV 183 Methanosaeta ...
AAB99700       111 AICkegDYVVLDKNAHYTSYVAAERAklNVAEVGyeeeyptykinlEGYKEVIDNLedk-gknVGLILLTHVDGeyGNLN 189 Methanocaldoc...
NP_615682       99 SMGkpgDWIVLDGLAHYSSYVAAERAglNVKVVPhagspe-yyldpEGYGTAIEEVtkesgkpPVLALVTYPDGsyGNIP 177 Methanosarcin...
jgi:MbarA_1638  99 SIGkpgDWIVLDGLAHYSSYVAAERAglNIKTVPhsgspd-yyldpEGYATAIEEVtkesgkpPALALVTYPDGsyGNLP 177 Methanosarcin...
NP_276195       93 ALCergDTVVVDGNAHYTTHLAAERNglEIVEVPstghps-yevtpEAYREVLEETidr--veVKLAVLTHVDGnyGNLT 169 Methanothermo...
AAM01446        94 ALCeegDVVVADSLAHYTTFVAAEKAgaTVREVPntghpe-ykvkvDEYARVIDEVedergdpPALALLTHVDSeyGNLA 172 Methanopyrus ...
NP_069020       93 GFK---GALVVDSLAHYTTYIAAELNglRVYEVPntgype-frieaESYTDVFEKVkeetgdyPAVALLTHADYkyGNLA 168 Archaeoglobus...
YP_565502      162 SIIekgDTVVVSTFAHYTEFLAVEGAggIVREAPvnehn---iltaESVAHKIDEVkretgklPALIMIDHFDYlfCNEH 238 Methanococcoi...
YP_001047525    95 TYVekgDPVIVTSLAHYTEFVAVEEAggIPLEVPkdekn---hitpDAAAEKIEAVilefsktPPLLFIDHVDYqfGNVH 171 Methanoculleu...
YP_001029791    95 TYVekgDPVLIGALAHYTSYLSVELQkgIVREIPktadn---hitaEDTANRIEDVvrefgiaPKLLYIDHVDYqfGNMH 171 Methanocorpus...
Feature 1                                                      #                                   
2E7J_A         165 DVKKIAKVCSeydVPLLVNGAYAIGrMPVSLkeigADFIVGSGHKSMAAsgPIGVMGMKEEWAEIVLRrsek-------- 236 Archaeoglobus...
jgi:Mthe_0102  184 DARRVAEICHeegVPLLLNGAYSIGrMPVSAreldADIVVGSGHKSMASsgPIGVLGAKQEYADIIFRrsks-------- 255 Methanosaeta ...
AAB99700       190 DAKKVGKIAKekgIPFLLNCAYTVGrMPVNGkevkADFIVASGHKSMAAsaPCGILAFSEEFSDKITKtsek-------- 261 Methanocaldoc...
NP_615682      178 DAGKIASVCHeyeVPLLLNGAYCVGrMPVSAkeigADFIVGSGHKSMAAsgPVGVLGVSEEYAPIVFRksvy-------- 249 Methanosarcin...
jgi:MbarA_1638 178 DARKIASVCHeydVPLLLNGAYAVGrMPVSAkeigADFIAGSGHKSMAAsgPVGVLGVSEEYAPIVFRksky-------- 249 Methanosarcin...
NP_276195      170 DARGVADVCRklgVPLLLNCAYSMGrLPVNLrelgVDFVVGSGHKSMAAsgPIGVLGMKSEWEDTVLRrsgr-------- 241 Methanothermo...
AAM01446       173 DAEKFVKICRkkgVPALLNCAYTMGrMDLSNlspkPDFMVGSGHKGMAAcaPCGVLAMREEWEEEVLRgsslrgdvsgre 252 Methanopyrus ...
NP_069020      169 DAKKVAEICRdygIPLILNTAYTSGiMEVSGretgCDFIVASGHKSWAAtaPIGILATTFEFAERVFRvsevrgnwsgra 248 Archaeoglobus...
YP_565502      239 DIYGIGKVAQeygIPFLYNGAYTVGiMPVNGqkigADFVVGSGHKSMASaaPSGVLATTEEWADKIFRttqmvgdvtgrk 318 Methanococcoi...
YP_001047525   172 DVAAITKVAHqydIPVLVNGAYSVGiMPVDGkalgADFVVGSGHKSMAApaPSGVLATTNEHAERVFRttqakgdvtgrt 251 Methanoculleu...
YP_001029791   172 DVKGIAKVAHqydIPVLYNGVYTVGiMPVNGkdlgVDFIIGSGHKSMAApaPSGILAATEERANEVFRttqmegdltgrk 251 Methanocorpus...
Feature 1                                                                                          
2E7J_A         237 yknkEVELLGCTArg-aTIITLMASFPHVRERikrWDEEVEKARRFAAEMEKL-GIKQLGDNPHNHDLMFFHAe-VLYEI 313 Archaeoglobus...
jgi:Mthe_0102  256 kknkEVELLGCTVrg-aGLLTMMASFPHVLERvgrWQEEVDKARWFSSELEEL-GIEQQGEKPHNHDLMFFKSe-RLYEI 332 Methanosaeta ...
AAB99700       262 fpvkEIEMLGCTSrg-lPIVTLMASFPHVVERvkkWDEELKKTRYVVDELEKI-GFKQLGIKPKEHDLIKFETp-VLDEI 338 Methanocaldoc...
NP_615682      250 skvkEVELLGCTArg-aTVMTMIASFPEVVKRvrnWDQEVENARWFSARLEDM-GFIQRGQKPHSHDLMFFEAp-GFYEI 326 Methanosarcin...
jgi:MbarA_1638 250 nkvkEVELLGCTArg-aTVMTLIASFPDVVKRvrnWDQEVENARWFSARFEEM-GFIQRGQKPHSHDLMFFEAp-RFYEI 326 Methanosarcin...
NP_276195      242 hekkELELLGCTSrg-aPLATLMASLPYVRERvsrWDGEVKKTRYLVSELEDIgGIEQLGVRPKEHDLVRFETp-VFHEI 319 Methanothermo...
AAM01446       253 wphkEVEMLGCTVmg-aPIVTMMASFPHVVERvkrWKEEVRKTRWFVKEMERIeGVRQLGERPKRHDLVKFETp-GFHEV 330 Methanopyrus ...
NP_069020      249 ftkkEVALFGCSPvyglPLVTLMASFPVVKERvkrWKEEVEKARWFVEEMEKIeGVMLLGERPKNHTLMNFETp-SFNLI 327 Archaeoglobus...
YP_565502      319 fgikEVEFLGCTLmg-aPLLSMMASFPHVKERtkhWDEEVKKSNYFINEFLRIeGNEVLSEFPRKHALSKVDTtgSFDKV 397 Methanococcoi...
YP_001047525   252 fgikEVEMMGCTLmg-vTVVGMMASFPHVKERvkhWDTEVAHSQAVVDALLSIeGTKVLSDYPRQHTLTRIDTreSFDKV 330 Methanoculleu...
YP_001029791   252 fgikEVGILGCSLmg-aPIVGLLASFPTVKARvehFDEELANSKIVVEALRFIeGTKILSEYPRKHTLTRVDTtgSFDQV 330 Methanocorpus...
Feature 1                                                                 
2E7J_A         314 SKkakggrffLYRELKSRKIHGikpgltRYFKLStyglsDEEVDYVLNAFKEIIE 368 Archaeoglobus fulgidus
jgi:Mthe_0102  333 SKsarkgryfLYHELKDRGICGikpgltRQFKLStylipREDLERVVSAFHEIIE 387 Methanosaeta thermophila PT
AAB99700       339 AKkdkrrgffFYDELKKRGIGGiragvtKEIKMSvygleWEQVEYVVNAIKEIVE 393 Methanocaldococcus jannaschii DSM 2661
NP_615682      327 SQkikkgryfLYKELKERNIHGiksgltKYFKLStfglgKEKLGVVADSFEEILK 381 Methanosarcina acetivorans C2A
jgi:MbarA_1638 327 SEkvkkgryfLYKDLKARNIHGiksgltKYFKLStfglgKEKLGIVADSFEEILK 381 Methanosarcina barkeri str. Fusaro
NP_276195      320 AAshprkgffLYEELKKRGIVGirrgqtKWFKCSiygmtEEQVQYVVDSFRDIVE 374 Methanothermobacter thermautotrophicus...
AAM01446       331 AEdhprrgyfLYEELKKRGVIGiqpgqtETIKASvygltDEQVEHVVRAFHEIAE 385 Methanopyrus kandleri AV19
NP_069020      328 AKkhrrkgyfLYHELKERGIFGvqpgmtRNVKLNvyglsWEEVERVAEAFKEIAE 382 Archaeoglobus fulgidus DSM 4304
YP_565502      398 AKthkrkgyfFSDELKKRGIAGefpgatRSWKMStyglsWDQIHYLSNSFIEIAD 452 Methanococcoides burtonii DSM 6242
YP_001047525   331 AQqhkkrgffLSSDLKKRGITGvipgstRVWKFNtfgltEKQIRHVGESFVEIAR 385 Methanoculleus marisnigri JR1
YP_001029791   331 AQthkkrgffLSSALGKKGITGiipgatKVWKFNtygmtKKQAEYVADTYLEIAE 385 Methanocorpusculum labreanum Z

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