1OBA


Conserved Protein Domain Family
GH25_Cpl1-like

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cd06415: GH25_Cpl1-like 
Click on image for an interactive view with Cn3D
Cpl-1 lysin (also known as Cpl-9 lysozyme / muramidase) is a bacterial cell wall endolysin encoded by the pneumococcal bacteriophage Cp-1, which cleaves the glycosidic N-acetylmuramoyl-(beta1,4)-N-acetylglucosamine bonds of the pneumococcal glycan chain, thus acting as an enzymatic antimicrobial agent (an enzybiotic) against streptococcal infections. Cpl-1 belongs to the CP family of lysozymes (CPL lysozymes) which includes the Cpl-7 lysin. Cpl-1 has a glycosyl hydrolase family 25 (GH25) catalytic domain with an irregular (beta/alpha)5-beta3 barrel and a C-terminal cell wall-anchoring module formed by six similar choline-binding repeats (ChBr's). The ChBr's facilitate the anchoring of Cpl-1 to the choline-containing teichoic acid of the pneumococcal cell wall. Other members of this domain family have an N-terminal CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain similar to that of the firmicute CHAP lysins and associated with endopeptidase activity. The Cpl-7 lysin is also included here as is LysB of Lactococcus phage, and the Mur lysin of Lactobacillus phage.
Statistics
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PSSM-Id: 119377
View PSSM: cd06415
Aligned: 11 rows
Threshold Bit Score: 234.985
Threshold Setting Gi: 90961782
Created: 13-Jun-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             #                         #                                 # #             
1OBA_A          6 DLFVDVSSHNGyditg--ileqmGTTNTIIKISESTTYl---------NRCLSAQVEQSNPIGFYHFARFGGDVAEAERE 74  Streptococcus ...
NP_814147      26 DQGVDWAIYQGeqgr----fgyaHDKFAIAQIGGYNASgiye--qytyKTQVASAIAQGKRAHTYIWYDTWGNMDIAKTT 99  Enterococcus f...
YP_535698      33 DHGVDAAVFQGasgk----wgyaRDKFMISQIGGTTTGwnlyd-qwtyPTQVSSTIAQGKRAHTYIWWQNVTSNSQADYV 107 Lactobacillus ...
YP_819093      29 TNGIDVASYQGdtanyfnsfkqvGDNFTMVKLGGRGGGegahysnpkaYAQIHNADAVGMQTGGYFWAQVGDSVSEATYS 108 Leuconostoc me...
YP_812161      38 SYGVDVSSYQPtstte---yskaGAQFVIVKVSEGTSYrnp-----naKAQVASAKANKLLPMAYHFANFGSSSSRAKSE 109 Lactobacillus ...
NP_964172      47 STGVDVSSWQGtkldq---qaksGAQFAVVKVSEGTNYqnp-----naQGQIQSAEQNNMMTMGYHYTHFGSNSNQAVQE 118 Lactobacillus ...
NP_964349       8 SYGVDISSFQGtdlla---mgrsGAKFAIVKTSEGLDYrnp-----kaQAQINSARNNGMMVSGYHYARFSNNANVAAQE 79  Lactobacillus ...
tigr:SAK_0653 154 DYFIDVSAYQQadltt--tcqqaGTTKTIIKVSESIAWl---------SDRHQQQANTSDPIGYYHFGRFGGDSALAQRE 222 Streptococcus ...
YP_001285906    5 TYGVDVASYQStdmaa---yhkaGASFAIVKLTEGTDYvnp-----kaSKQVDSSRANHLYTHAYHFARFGSSVSQAKKE 76  Lactobacillus ...
AAQ06663       10 SYGVDVASYQSttv------nyaGAKFAFVKLTEGTEYtnp-----kaEAQIKSAKAHGLMVMGYFYANHSASVSKAMAE 78  Lactobacillus ...
ZP_03072731     5 KTVIDLASFQShltvd--dykaiDADYAIVKTTESTNYvnp----yirSLIDRSAGGGIKGFAFYHFGRFHNDTQAVAEA 78  Lactobacillus ...
Feature 1                              # #                                            #           
1OBA_A         75 AQFFLDNvpm----qvKYLVLDYEddpsg--------------daqaNTNACLRFMQMIADAGYKPIYYSYKPFTHDNVD 136 Streptococcus ...
NP_814147     100 MDYFLPRiqt---pknSIVALDFEhgalasvpdgyggyvssdaekaaNTETILYGMRRIKQAGYTPMYYSYKPFTLNHVN 176 Enterococcus f...
YP_535698     108 LNYFLPKiqt---pkgSIVALDVEsg-------------------yqNTQAIAHAIQRIKDAGYTPMLYGYKNYLVNNTD 165 Lactobacillus ...
YP_819093     109 AQLAVQDaqnaglakgSYIAMDYEagaga--------------nkanNTTAILAFMDQIYASGYKPMFYSYTSYVNSYVD 174 Leuconostoc me...
YP_812161     110 ADYAIASakavslpagSYLACDWEtssgnst----------sgsrsaNTSAIMAFMKKVKAAGYQPLLYSGSYLLNNAIN 179 Lactobacillus ...
NP_964172     119 GNYAVNSaqqaglpqgSYLATDWEqdvnnnt----------ngsvaaNTNAITSFMDTVHDGGYNPMLYSSEWLLKNKVD 188 Lactobacillus ...
NP_964349      80 GNYAVSSakqvgiepgSYLACDYEqgsgnet----------ggnreeNTTAILAFLNTIVSAGYKPLLYSGAELMKNKVN 149 Lactobacillus ...
tigr:SAK_0653 223 ADLFLSNlps---kkvSYLVIDYEdsasa--------------dkqaNTNAVIAFMDKIASAGYKPIYYSYKPFTLNNID 285 Streptococcus ...
YP_001285906   77 ATYFIKEakkedisqkRIVWLDWEsgsgntv----------tgskasNTSAILAFMDAIKAAGWRPGLYSGASLLRTAID 146 Lactobacillus ...
AAQ06663       79 AKYAVAKakaygipagSYLADDWEqgsgnsv----------ngstsaNTDAVLAAMQAIKEAGYKPLIYSGAYNLRNHLS 148 Lactobacillus ...
ZP_03072731    79 NYFIANSktqanvqpgTLMILDAEis-------------------nmPTSSVIAFLNTVRNAGYHPGFYTYKYLLPNFNL 139 Lactobacillus ...
Feature 1                     #                              #          #      
1OBA_A        137 YqqilaqFPNSLWIAGYglnd-----gtanFEYFPSMDGIRWWQYSSn----pFDKNIVLL 188 Streptococcus phage Cp-1
NP_814147     177 YqqiikeFPNSLWIAAYpidgv---spyplYAYFPSMDGIGIWQFTSayiaggLDGNVDLT 234 Enterococcus faecalis V583
YP_535698     166 Lsy--lsTLCQLWLAEYpnyav---tpepnYNYFPSFNNIGIFQFTStyvaggLDGDIDLT 221 Lactobacillus salivarius subsp. s...
YP_819093     175 LsrinarYPNALWLAWYlttah---qatppMQYFPNYSNVKIWQYADnh--ygVDGNVMVV 230 Leuconostoc mesenteroides subsp. ...
YP_812161     180 MstvtstYPNSLWVAYYtssgr---idapdFSYFPSMNGVIIWQFTEnwkglnVDGNISVL 237 Lactobacillus delbrueckii subsp. ...
NP_964172     189 TnkisekYPNALWVAKYktngr---ednpdYNYFPSMDNVAIWQYTQnwrgqnVDGNVNVV 246 Lactobacillus johnsonii NCC 533
NP_964349     150 TakilakYPNCLWVAAYpykga---tntadFNYFPSMEGVIIWQFTDnwrgmnVDGNINLL 207 Lactobacillus johnsonii NCC 533
tigr:SAK_0653 286 YqkiiakYPNSIWIAGYpdyev---rteplWEFFPSMDGVRWWQFTSvgvaggLDKNIVLL 343 Streptococcus agalactiae A909
YP_001285906  147 TaqvvkkYGTCLWVASYptmaa---vssadFRYFPSMDGVAIWQFTSnwkglgVDGNLALV 204 Lactobacillus phage LL-H
AAQ06663      149 IsrivksFGTCLWVASYkvsgr---qdyadFNYFPSMDGVAIWQFTDnyrglgVDGNITLI 206 Lactobacillus helveticus phage f-...
ZP_03072731   140 Eai--hpNMDMFWLAAYplangeaagknpdFNYFPSANYVDMWQHTDnllgynVDGSITLT 198 Lactobacillus reuteri 100-23

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