1TVC


Conserved Protein Domain Family
MMO_FAD_NAD_binding

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cd06210: MMO_FAD_NAD_binding 
Click on image for an interactive view with Cn3D
Methane monooxygenase (MMO) reductase of methanotrophs catalyzes the NADH-dependent hydroxylation of methane to methanol. This multicomponent enzyme mediates electron transfer via a hydroxylase (MMOH), a coupling protein, and a reductase which is comprised of an N-terminal [2Fe-2S] ferredoxin domain, an FAD binding subdomain, and an NADH binding subdomain. Oxygenases oxidize hydrocarbons using dioxygen as the oxidant. Dioxygenases add both atom of oxygen to the substrate, while mono-oxygenases add one atom to the substrate and one atom to water.
Statistics
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PSSM-Id: 99806
View PSSM: cd06210
Aligned: 10 rows
Threshold Bit Score: 358.193
Threshold Setting Gi: 21239751
Created: 17-Apr-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1TVC: Methylococcus capsulatus Methane monooxygenase binds dihydroflavin-adenine dinucleotide, contacts determined at 4A
    View structure with Cn3D
  • Comment:contacts lie primarily in the N-terminal region

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                       #           ####             ####
1TVC_A        10 SFEAE-----VVGLNWVSSn--TVQFLLQKrpdec-gnrgVKFEPGQFMDLTIPGTDvSRSYSPANLPnpEGRLEFLIRV 81  Methylococcus c...
CAB45257     103 NWLAE-----ILACDRVSSn--VVRLVLQRsrpma-arisLNFVPGQFVDIEIPGTHtRRSYSMASVAe-DGQLEFIIRL 173 Methylosinus tr...
AAZ81979     106 NWLAE-----ILACDRVSSn--VVRLVLQPltadgaarisLNFVPGQFVDIEIPGTHtRRSYSMASVAe-DGQLEFFIRL 177 Methylomonas sp...
CAJ26296     106 DWQGE-----IVAVERISSn--VARLQIEPkdpetgaaisIPFVPGQYVDIEIPGSSvSRSYSMATTSt-QSRLDFLIRL 177 Methylocella si...
BAE86880     109 CFEAE-----ITEVSQISSn--VYRLCLKRigt----nqqIKLAAGQYYELQIPGTEtWRSYSPANTEnnDGVMEFLIRV 177 Methylomicrobiu...
YP_775976    104 VRTAR-----IAALDAIARdtmRVELQIEPddaf---gvaAEFEAGQFAELEVPGSGlRRPYSLANTSnwDGRLEFLIRL 175 Burkholderia ce...
AAM19732     106 VTATEak-tsVVSVERLSSt--VYRLVLQAldaeg-mparFDFVPGQYVEISTADSLeTRAFSLANLPndAGLLEFLIRL 181 Pseudomonas but...
YP_001234921  99 TREAVieriaPAGASAVSL---ALRLRPDAelg-----qaADFVPGQYMEVAIPGTDiRRAYSLANLPnwDGRVEFLIRL 170 Acidiphilium cr...
YP_001539371 120 RREGA-----IEAVTSIAHd--TVWLRVQAapdaa-agtgVEFDPGQFVELQLPGDDrRRAYSMANTPnwDGELEFLIRL 191 Salinispora are...
NP_863592    107 EREAE-----VMDVTMVATgtrRLLLRLQPdeq----ggaAEFEAGQFMEIQVPGSDaRRPNSLANNTnwNGDLEFFITL 177 Acidithiobacill...
Feature 1        #   ###                        #                                #  #            
1TVC_A        82 LPEGRFSDYLRn----dARVGQVLSVKGPLGVFGLKERGM--------------APRYFVAGGTGLAPVVSMVRQMQEWT 143 Methylococcus c...
CAB45257     174 LPDGAFSKFLQt----eAKVGMRVDLRGPAGSFFLHDHGG--------------RSRVFVAGGTGLSPVLSMIRQLGKAS 235 Methylosinus tr...
AAZ81979     178 LPDGAFSKFLQt----eAKVGMRVDLRGPAGSFFLHDHGG--------------RSRVFVAGGTGLSPVLSMIRQLGKAS 239 Methylomonas sp...
CAJ26296     178 LPDGQFSNFLTm----aAKPGLTVKLRGPFGAFNLRENGF--------------RPRYFVAGGTGLSPVLSMIRYMQQEQ 239 Methylocella si...
BAE86880     178 LPDGKFSKFLMh----dAQIGQTVHLKGPSGVFGLKENGF--------------TPRFFVAGGTGLAPILSMVRHMKAWE 239 Methylomicrobiu...
YP_775976    176 RAGGWFSTYLRe----rARPGDRLTVRVPMGGFGLFADSL--------------RPRWFVAGGTGLAPILSMLRRMAEYQ 237 Burkholderia ce...
AAM19732     182 VPGGYYAAYLEq----rAAAGQTINVKGPFGEFVLREHELvedftlpadspargGTIAFLAGSTGLAPLASMLRELGRRG 257 Pseudomonas but...
YP_001234921 171 VPGGAFSTWLGt----eAKPGDALSLRGPLGRFVLDDTSP--------------RPRCLVGGGCGLAPLLSMLRHLAEFQ 232 Acidiphilium cr...
YP_001539371 192 HEGGYFSGYLAdllagrRPLGERIVLHGPTGAFALRESGL--------------RPRWFVAGGTGLAPLLSLLRRMAEWG 257 Salinispora are...
NP_863592    178 RPGGAFSTYLEs-----ALVGDRLNIRGPLGTFTLRENGL--------------RTFGSLVAGQGLVPLLSMLRRMADWG 238 Acidithiobacill...
Feature 1                   #   ##                         #                                     
1TVC_A       144 apNETRIYFGVNTEpELFYIDELKSLErsMRNLTVKACVWHPSgdWEGEQGSPIDALREDLEss--dANPDIYLCGPPGM 221 Methylococcus c...
CAB45257     236 dpSPATLLFGVTNReELFYVDELKTLAqsMPTLGVRIAVVNDDggNGVDKGTVIDLLRAELEks--dAKPDIYLCGPPGM 313 Methylosinus tr...
AAZ81979     240 dpSPATLLFGVTNReELFYVDELKTLAqsMPTLGVRIAVVNDDggNGVDKGTVIDLLRAELEks--dAKPDIYLCGPPGM 317 Methylomonas sp...
CAJ26296     240 hpQEAKLFFGVTHQhELFYLEELKKLEesMPNFSAHVAVMQPDgnWQGSRGTVVDDLLKHLEgt--kAAPDIYMCGPPGM 317 Methylocella si...
BAE86880     240 epQQSVIYFGVNTEdEIFYVDELNRLAdeMPNLTVRNCVWKATdtWNGEKGSVVDILRRDLVet--gAKPDLYLCGPPGM 317 Methylomicrobiu...
YP_775976    238 emGDARLFFGVNEEsELFMLDELERLRaeLPQLRVDVCVWRPGveWTGLRGTPVDALRAALAha--pVLPDVYVCGPPPL 315 Burkholderia ce...
AAM19732     258 fnGECHLFFGMQDTaTMFYEKELRDIKrtLPGLTLHLALMVPSaeWEGYRGNAVAAFKEHFAas-sqIPENVYLCGPGPM 336 Pseudomonas but...
YP_001234921 233 dmQETHLIFGANREaELFATDEIAALAaqLPCLTVTTAIWHPEgdWSGFTGTSAEALDSWLSna--aTPPDIYVCGPPKL 310 Acidiphilium cr...
YP_001539371 258 epHPARLLYGVTAEhDLPDLPVLAEITdaLPGFELVPCVWQPGptWTGRCGTPADVLAEDLAglrvtAAPDVYVCGPPLL 337 Salinispora are...
NP_863592    239 emLPARLYFGARYEdELFCQEEIRQIQdkLPQLQVKICLSRPGnhWMDYRGSVVDALRDDLGsl--aTLPDLYVCGSTRL 316 Acidithiobacill...
Feature 1                            # ##
1TVC_A       222 IDAACELVRSRGIPGeQVFFEKFL 245 Methylococcus capsulatus
CAB45257     314 IEAAFAAAATAGVPKeQVYLEKFL 337 Methylosinus trichosporium
AAZ81979     318 IEAAFAAAATAGVPKeQVYLEKFL 341 Methylomonas sp. GYJ3
CAJ26296     318 IDATFAAAANYGVPKdHVYVEKFL 341 Methylocella silvestris BL2
BAE86880     318 VDATYAVCTEVGIPKeHVYLEKFL 341 Methylomicrobium sp. NI
YP_775976    316 VEAAREVAIAAGVPDaQFASERFT 339 Burkholderia cepacia AMMD
AAM19732     337 IAAALGACRELGIPDnRVHREEFV 360 Pseudomonas butanovora
YP_001234921 311 VEAVVTTARWHGVPEaQILAEQVQ 334 Acidiphilium cryptum JF-5
YP_001539371 338 VDAVQQVVRAAGLPDsHVVTERVL 361 Salinispora arenicola CNS-205
NP_863592    317 VQGVTELALSQGLPDsCLQFERFL 340 Acidithiobacillus ferrooxidans

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