Conserved Protein Domain Family
bifunctional_CYPOR

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cd06206: bifunctional_CYPOR 
These bifunctional proteins fuse N-terminal cytochrome p450 with a cytochrome p450 reductase (CYPOR). NADPH cytochrome p450 reductase serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99802
View PSSM: cd06206
Aligned: 31 rows
Threshold Bit Score: 410.496
Threshold Setting Gi: 21449376
Created: 15-May-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:determined by homology to E. coli sulfite reductase

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                       #                                 
P14779        666 NVVASKELQQpgs--arsTRHLEIELPk----------eASYQEGDHLGVIPRNYeGIVNRVTARFGLDASqqirlea-- 731  Bacillus mega...
XP_001905185  572 TIAAIRQLADtsv--gpaKYHVDVRLPp----------gCHYRAGDYLVVQGRNStESVHRTIARFSLSPAdtmtvq--- 636  Podospora ans...
AAM54108      662 VVLENRELVRgpd--agsKRHLELRLPd----------gTSYRTGDYLSVLPQNHpDLVRRAVARLGTRAErvvtves-- 727  Actinosynnema...
BAC55896      691 LVVENTLLTQagv---paKRTIRFKLPt----------dTQYKCGDYLAILPVNPsTVVRKVMSRFDLPWDavlriekas 757  Aspergillus o...
YP_366494     679 SIVDNRELVDisvagsrsKKHIELKLPe----------aMTYRSGDYLAVLPRNAkNNVDRVLRRFRVSWDtqvvieg-- 746  Burkholderia ...
YP_001106399  681 TVTEVADLTApdy--prtKRFVRLALPe----------gTAYRTADHLAVLPVHDaALVERAAGVLGVDLDtvldir--- 745  Saccharopolys...
YP_001545288  668 SVLENRELVDlssplgrsKRHIELRLPn----------eLQYQAGDYLAILPQNHpSLIERACKHFGLKPEqtiilha-- 735  Herpetosiphon...
XP_001908745  714 LVLVNKVLAKkgl--gpeKRQIDILLPp----------gVQYRSGDYLAIQPFNPrDSIRRVLSRFGLHPDdlitvsg-- 779  Podospora ans...
XP_001269930  677 SVIENKLLTAsgv---kaKRHLELALPp----------nAEYTVGDYLHILPMNPpVTVQRAMGFFRLAEGtymkvt--- 740  Aspergillus c...
NP_821750     675 TVVANEELVSdat--glwDFSIEPPRPaaksitielpdgVTYDTGNHLAVFAKNEpVLVNRALARLGVDRDqvlrldq-- 750  Streptomyces ...
Feature 1                                                                                         
P14779        732 --eeeklaHLPLAKTVSVEELLQYVELQDPvtrtqlramaaktvc-----pphkveleallekqaYKEQVLAKr---lTM 801  Bacillus mega...
XP_001905185  637 ---sskkdFLPSQPMAIEHFLRQRVELAAPitkrqlltlslhaeds----spekahllnltqdsaYEDLLTNRh----SV 705  Podospora ans...
AAM54108      728 --saptglVPVGRALRVDELLTRCVDLSAPagagvvarl------------------aercpcppERAELAATtg--aTL 785  Actinosynnema...
BAC55896      758 pssskhisIPMDTQVSAYDLFATYVELSQPaskrdlavladaaavd----petqaelqaiasdpaRFAEISQKr---iSV 830  Aspergillus o...
YP_366494     747 --tssnprLPLGQAIGCGELFSSYVELAVPatrsqvsslaaatrc------ppekvelerlsadgFECEILGKr---tTV 815  Burkholderia ...
YP_001106399  746 --akrpgrLTFDRSLTVRELLSHHVELQDPptpdgldalaal-----------npcpperaalrgLAEEARSGtadhrTL 812  Saccharopolys...
YP_001545288  736 --trgaanLPIDRPISLGELLSSHVELATPatqrdlellaqknvcp----shqihlaalaadherYTTEILQKr---lSL 806  Herpetosiphon...
XP_001908745  780 -ttkehlkSESGGPISVFELIGTRVELANPasqrqvahlaslssg------leadklrvlasdeeYPTSVLAKr---fSV 849  Podospora ans...
XP_001269930  741 --gglstvLPIGKEISVEEALSRYVELSQPatqkilerislstp--------vltereklshqaaSSTSLQDKr---iSI 807  Aspergillus c...
NP_821750     751 -pgggrthLPVGTPVTTGLLFTEFVELQDVatrsqiqelaehtqcpwtrpqlqaytadtaeaeerYQKEILGKr---vSV 826  Streptomyces ...
Feature 1                                     ####                                   # #          
P14779        802 LELLEKYPac--eMKFSEFIALLPSIRPRYYSISSSPRvde---------------------kqASITVSVVSGEawsg- 857  Bacillus mega...
XP_001905185  706 LDVLSTIPtl--aLPFGVFIDLLPPLAPRVYSISSSPLspssgt--------------lqsglvASITFDLFQSPalss- 768  Podospora ans...
AAM54108      786 LELLERFPsc--aVDLALALELLPAPRTRLYSISSAAEe-------------------------QRAEVALTVSV----- 833  Actinosynnema...
BAC55896      831 LDLLLQYPsi--nLAIGDFVAMLPPMRVRQYSISSSPLvdp---------------------teCSITFSVLKAPslaal 887  Aspergillus o...
YP_366494     816 MDLLERFGsv--dLSLEKFLDMLPALKARQYSISSSPLwka---------------------dhVTLTVAVVDAPalsg- 871  Burkholderia ...
YP_001106399  813 WDLIEDHPalrdaLSWSALLELLPATRPRQYSVSSSPAvdp---------------------rhVDLMVSVLRAParsg- 870  Saccharopolys...
YP_001545288  807 LDMLEQYPss--vLDFGEFLELLPAMRVRQYSISSSSLvnp---------------------nqASLTVAVVDAPawsg- 862  Herpetosiphon...
XP_001908745  850 LDLLEDFPsc--kLSFASYLDMLSPLSPRQYSISSSPLaqkplpdqgsgwsseegvdlekasltATLTYDVYNSPlmshp 927  Podospora ans...
XP_001269930  808 LDLLEHAPss--tISFADFISMLQPLRARPYSISSSPLnca---------------------rsCSLTVSIVDNPeptar 864  Aspergillus c...
NP_821750     827 LNLLERFPav--eLPLAVFLEMMGPIRPRFYSISSSPLanp---------------------rhVRLTVGLLEGPalsg- 882  Streptomyces ...
Feature 1                ####                                                                     
P14779        858 --ygeyKGIASNYLAELqeGDTITCFISTPQSEFTLPkd-peTPLIMVGPGTGVAPFRGFVQARKQLke-------qGQS 927  Bacillus mega...
XP_001905185  769 --hgtfNGVASSYLSSRkiGDEISCLVRPTTLPFQLPkd-isKPIAMVAAGSGIAPMRGLLQERAELmk-------qGGE 838  Podospora ans...
AAM54108      834 ------TGVTSGYLSRVrpGDRVAVGIASPPESFRPPad-ntVPVVLIAAGTGIAPFRGFLRARAAL----------GGE 896  Actinosynnema...
BAC55896      888 tkedeyLGVASTYLSELrsGERVQLSVRLSHTGFKPPte-lsTPMIMACAGSGLAPFRGFVMDRAEKirgrrssgsmPEQ 966  Aspergillus o...
YP_366494     872 --ngrhEGVASSYLARLntGDSLSVAVRPSNARFRPPae-pdLPMILICAGSGIAPFRGFLQERALQkq-------rGEN 941  Burkholderia ...
YP_001106399  871 --rgefRGAGSRHLSEVrpGDTVLARVQPCREDFRVAp---dEPLIMVAAGTGLAPFRGVIADRRERva-------nGAR 938  Saccharopolys...
YP_001545288  863 --kgqfYGTGSSYLARLqvGDQIAVSLRQPHIPFRPPsa-nsTPLLMICAGTGLAPFRGFIQERVARqg-------qGEA 932  Herpetosiphon...
XP_001908745  928 -etksfNGVSSSYLASLqpGSRLRCFVRKTNAPFRLPsd-paTPVVMVAAGTGVAPMRAFLQERAAVagar--ggyhKSG 1003 Podospora ans...
XP_001269930  865 --etrvLGISTHYITRLkpDQRILCSIRPSNSGFTFPdnpetTPMVMICVGSGIAPFRAFVQERVCRae-------kGQK 935  Aspergillus c...
NP_821750     883 --dgryRGTCSSYIAGLesGDVFYGYVRVPSPTFAPPad-paTPLLLIGPGTGIAPLRGFLEERAHQha-------hGTQ 952  Streptomyces ...
Feature 1                                                                                         
P14779        928 LGEAHLYFGCRSPhEDYLYQEELENAqseGIITLHTAFSRMPnq--pkTYVQHVMEqdgKKLIELLDQGAHFYICGDGsq 1005 Bacillus mega...
XP_001905185  839 FGEAVLFFGCRDEeKDYLYKEELERWergGVVRVVPCFSRPGgk--kgRYVTDALWeerDRMWEVVEKGGRIFTCGSAar 916  Podospora ans...
AAM54108      897 PGPALLLFGCRGPeLDDLYAEEFAALg--DWLEVDRAYSRHPdg--evRHVQHRLWqrrDRVRELVDAGARVYLCGDAtr 972  Actinosynnema...
BAC55896      967 PAKAILYAGCRTQgKDDIHADELAEWekiGAVEVRRAYSRPSdgs-kgTHVQDLMMedkKELIDLFESGARIYVCGTPg- 1044 Aspergillus o...
YP_366494     942 VGTSLLFFGIDDPdVDFLYRDELDEWarcGVVEVMPAYSNRPee--gaRFVQDKVWlerERISALFSQGATVFVCGDGkn 1019 Burkholderia ...
YP_001106399  939 QAPALCYFGCDAPdADYLHSAELRAAesaGAVAMRPAFNEAPvg--gqCFVQHRIAaeaGEVWALLESGARVLVCGDGrh 1016 Saccharopolys...
YP_001545288  933 LGPNALFFGCDHPeVDLLYHEQIQAWqkaGVLEFFPAFYRQPvg--evSFVQHRLWqerQYVWSLIEQGAVIAVCGDGrs 1010 Herpetosiphon...
XP_001908745 1004 LGPAMLYYGCRDFeEDYLYKEELGQWekeGVVRVRAAFSRRAgeggrtGHVDELIWedrEELKGLIKQGAKILVCGSAgr 1083 Podospora ans...
XP_001269930  936 LAPGLLFIGCKSPkHDRLYGSELDQLeeaGIVTVRYAYSGLPy----rKHVQDKLWderETLVDMYKGEARFYLCGPVe- 1010 Aspergillus c...
NP_821750     953 VGLSQVFVGCRHPeHDYFYRQEMQDWeqaGIAQVHTAFSAVTgh--paRFVQDAIVgaaDTVWQAIQDGAYVYVCGDGrr 1030 Streptomyces ...
Feature 1                                                                    
P14779       1006 MAPAVEATLMksyadvh----------------qvseadaRLWLQQLee-kgRYAKDVW 1047 Bacillus megaterium
XP_001905185  917 LGRSAGEVWRriweeksg---------------qggeregEEWLEGIkg-dgRYVCDVY 959  Podospora anserina DSM 980
AAM54108      973 VGPAVEEVLGrig-------------------------pgAGWLDALra-ggRYATDVF 1005 Actinosynnema pretiosum subsp. aur...
BAC55896     1045 VGNAVRDSIKsmflerreeirriakekgepvsdddeetafEKFLDDMkt-keRYTTDVF 1102 Aspergillus oryzae
YP_366494    1020 MAPAVRATLGriyqett----------------gendesaSAWIDTMerehgRYVADVF 1062 Burkholderia sp. 383
YP_001106399 1017 MAPGVREAFRgiyrertp---------------gaddasaHEWLQAMia-ggCYVEDVY 1059 Saccharopolyspora erythraea NRRL 2338
YP_001545288 1011 MAPAVRETLAriyaeat----------------gseqtagMAWIAEIeq-agRYVADVF 1052 Herpetosiphon aurantiacus ATCC 23779
XP_001908745 1084 LGRSTAEVCLriyedefp---------------drgregaEEWLGKMke--eRYVSDVF 1125 Podospora anserina DSM 980
XP_001269930 1011 LRDSVLAVARriymatvean-----------gevrgedeyQNWLAKMnk--gRFAADTF 1056 Aspergillus clavatus NRRL 1
NP_821750    1031 MAPAVREALAaiyrkht----------------gsddeaaQQWLAQLea-deRYQQDVF 1072 Streptomyces avermitilis MA-4680

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