Conserved Protein Domain Family
SiR_like2

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cd06201: SiR_like2 
Cytochrome p450- like alpha subunits of E. coli sulfite reductase (SiR) multimerize with beta subunits to catalyze the NADPH dependent reduction of sulfite to sulfide. Beta subunits have an Fe4S4 cluster and a siroheme, while the alpha subunits (cysJ gene) are of the cytochrome p450 (CyPor) family having FAD and FMN as prosthetic groups and utilizing NADPH. Cypor (including cyt -450 reductase, nitric oxide synthase, and methionine synthase reductase) are ferredoxin reductase (FNR)-like proteins with an additional N-terminal FMN domain and a connecting sub-domain inserted within the flavin binding portion of the FNR-like domain. The connecting domain orients the N-terminal FMN domain with the C-terminal FNR domain. NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria in which they participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2 which then transfers two electrons and a proton to NADP+ to form NADPH.
Statistics
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PSSM-Id: 99798
View PSSM: cd06201
Aligned: 14 rows
Threshold Bit Score: 364.728
Threshold Setting Gi: 86130612
Created: 22-May-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
  next features
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Comment:determined by homology to 1DDG, E coli sulfite reductase contacts to FAD

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
YP_001268056 444 GGSPLLGLETVNRQCPQ----------AFARWGHALGd----VLGQALALVHTPqq---prTHSLELTERIAYGeqvnaP 506 Pseudomonas put...
YP_001418281 443 GFPQLQPLKRIDRRSAQ----------EFAQWGRDLGs----SLGHDLVLEHTAer---pkTLALELASREDYGaaigaP 505 Xanthobacter au...
YP_001768532 439 GWPRLMPPAWIDRQSAQ----------GFAQWGRDLGra--lGHGRDLVLAHASpv---ppTAPYTLVGRDDYDiasatP 503 Methylobacteriu...
EDU12822     489 TLPRTMPLTLVERDDYGgdpramtsvlRFAPAGRDGRtgradRAGRDEHDERDGrerrqerEERLEDAGRRAAW-----W 563 Burkholderia ps...
EDT29252     444 GWKTLIPFDTVDHQSTQ----------DFTRWGRALGa----ALGIELELVHQAra---paALPLTLLSRHDYGaevqsP 506 Oligotropha car...
ZP_01546235  446 GVAELLPMATVDRQSTQ----------QFAAWSHDLGk----AISIDLHVEHTAdl---pgLKEWECVARKDFGqayqtP 508 Labrenzia aggre...
YP_988268    442 GWPTLLPLERIHQQSAQ----------EFARWGRTLSq----ALGLPLQIDYQPrl---prTVALTLAARQDFPggageP 504 Acidovorax sp. ...
YP_941737    444 GWNPLTNIDIIDKQSTQ----------AFNRWGTVVGs----LMGLDLDLIYCPvh---lnTQLLQLVDRVDYGaevnaP 506 Psychromonas in...
YP_767868    440 GWASLIPFDTVNRQSPQ----------DFARWGRLLAe----ALGLDFELKHQQia---pkTWRLPLISRRDYGasvqaT 502 Rhizobium legum...
ZP_01076314  467 GFKQMLPLTAIDKFCQT----------SLETWIENVStc--lNQSLCLKLDKKKi-----sPFQMQLVEQSHYGdevdaP 529 Marinomonas sp....
Feature 1                                               #           ####            # #    ####  
YP_001268056 507 THVLRFNArg--------------------glPNFLAGDLVGILPPGSPIPRFYSLASGSqdGVLEICVRKHSGGMCSEF 566 Pseudomonas put...
YP_001418281 506 VAILRFRGpgrga--------------aagrlPAFEPGDLLGILAPGADMPRFYSLASSSrdGMVEICVRLRAGGLCSTF 571 Xanthobacter au...
YP_001768532 504 VAVLRLAPadg------------------getPPFRAGDLLGILAPGTEVPRSYSLATSSedGVLEICVRRHTGGLCSGF 565 Methylobacteriu...
EDU12822     564 TAFARRRL------------------------PSFETGDLLGVVPPGEASPRYYSLASASsdGIVEICVRRHPHGVCSRY 619 Burkholderia ps...
EDT29252     507 TVILRFELpkirliq----------rmtghgfGRFAAGDLLGVLPEGSVVPRFYSLASAHrdGFVEIVVKKHPAGLCSGQ 576 Oligotropha car...
ZP_01546235  509 KSILRFRAvqtkpgrf---------glsraggPSFKAGDLIGILPKGSDLPRFYSLASSAsdGFLEIAVSKHPGGLCSGQ 579 Labrenzia aggre...
YP_988268    505 AAILRFALpar-------------------glPRFAAGDLIGIVAPGQAVPRYYSLASGTrdGFVEICVRRMPGGVCSSH 565 Acidovorax sp. ...
YP_941737    507 TAILRFIPaekktatff------nkirrkprlPQFSAGDLVGILPTESDGARFYSLASASknRQLEICVHQHPQGICSNY 580 Psychromonas in...
YP_767868    503 TAILRFALpkvslwq----------rltgkgfPRFEAGDLIGIVPQGSDLPRFYSLASGTkdGFLEICVRQQVGGLCSSQ 572 Rhizobium legum...
ZP_01076314  530 VRILRFKAatdpetvlnpaideafalssdmpwPEFEVGDLVGIMPPGSDFVRYYSLASCDqdGMLEICVRKQVEGECSGF 609 Marinomonas sp....
Feature 1                                                                                        
YP_001268056 567 LHGLdIGAQIDAFIQPNPQFRPAsGTHPVILIGAGTGIGPLAGFIRNNkARHPMHLYWGGRNPaSDFLYEPELNQYLsdr 646 Pseudomonas put...
YP_001418281 572 LHGLaPGDRVEAFVRANPVFRPAkGRAPLILIGAGAGIGPLAGLVRANaAGRPVHLYWGGRSPsSDFLYEHELAQHLaek 651 Xanthobacter au...
YP_001768532 566 LHDLpIGGSVRAFIRANPEFRPApGRAPLVLVGAGTGIGPLIGFVRANrARRPIHLYWGGRRPeSDYLYARELAEHLaag 645 Methylobacteriu...
EDU12822     620 LTGLqPGDTIEAFVRPHARLRPHaGAAPVILIGAGTGIGPLIGFIRHNaARRPMHLYFGARNAnDGFPYRDELDGLVrdr 699 Burkholderia ps...
EDT29252     577 LFELrPGATINAFLKRSPAFHPDhSRAPLILIGAGTGIGPLAGFVRANaRYRPIHLFFGMRHPdSDFLYAEELAEWQrdg 656 Oligotropha car...
ZP_01546235  580 LCDLgPCDRIAGFIQPNARFNMPrGKAPVILIGAGTGVAPLVGMVRANdALKPAYLFAGSRDPqSDQLYREEIDTFRksg 659 Labrenzia aggre...
YP_988268    566 LHALqAGDSVQAFIRSNPGFVLPaGRAPVLLIGAGTGVAPLAGFIRDNtRHRPMHLYYGARHPeRDFYFGTELPSWQadg 645 Acidovorax sp. ...
YP_941737    581 LHKLqLGECIKGFIRENPTFRPRlEQSSIILIGAGTGIAPLIGFIRNNhTCHPMRLYWGGRDPnSDFLYRAELEQYLqdk 660 Psychromonas in...
YP_767868    573 LTALkPRDTVAAFVRPNPSFRPArGGKPVILIGAGAGIGPLAGFARRNrARRPMHLYFGTRHPaSDALYAEELSYWKkdg 652 Rhizobium legum...
ZP_01076314  610 LHGLkEGDSIQAFIQKKASFRPAqDASSVIMIGAGTGMAPLQGFIKQNeKQVPYYLYWGGRLQnSDFIYEDNLSQALats 689 Marinomonas sp....
Feature 1                                                                                        
YP_001268056 647 RLTQLHAAFSQVQe-rSYVQDRLISDaLALRRLIEKGaQVLVCGSREMAKGVMQALDe--VLAPLHLSVLIlkaqGRYRE 723 Pseudomonas put...
YP_001418281 652 RLTSLQTAFSRGPdgrAYVQDRIAADaPRLRELVRQGaQILVCGGRDMAEAVTRAFEp--VVRPLGLDLATlkshGRYVE 729 Xanthobacter au...
YP_001768532 646 RLTALRTAFSRRPg-gGYVQDRLAADaALVREQVHRGgQVLVCGGRSMAQAVRRVLDg--AVQPLGLDVTTlrsrRRYAE 722 Methylobacteriu...
EDU12822     700 RLRALTTAFSRAEr-gAYVQDRLVADaRNLRELVAHGaQIMVCGGRAMADGVARAWErilADSGSSVAQLKq--qGRYVE 776 Burkholderia ps...
EDT29252     657 RLRQLATACSRGRm-pRYVQDTLREEsAEIIRLVQAGaRVMVCGGRDMAAGVSAALTdilATAGLTPALLKa--eGRYAE 733 Oligotropha car...
ZP_01546235  660 RVRNVSYSFSRVPg-gAYVQDALRANaEELRRMVRQGaDIMICGGTSMAEGVSKALDdvlGPLGLTVQKLRa--dGRYQE 736 Labrenzia aggre...
YP_988268    646 RLAGLHTTFSRTPg-gGYVQEILRRDaTQVLALIAQGaLVRVCGSRAMAQGVAQVLDallGPQGQSLAQLKe--qGRYAE 722 Acidovorax sp. ...
YP_941737    661 RLTELVTAFSRTEe-sCYVQDKVLADaPKIQALISEGaQILVCGGRPMSKCVAAAITrviSPLQITLQDLKe--qGRYLE 737 Psychromonas in...
YP_767868    653 RLTSVSTAFSRTAs-pAYVQDIVRKDgERIGKLIAAGgQVLICGSRDMAAAVAAVLAdilAAQGFNLALLKa--tGRYAE 729 Rhizobium legum...
ZP_01076314  690 KLKQLRLAFSRSTk-pQYVQNLLTDDaKDISKRILEGaQIMVCGSKVMADGVRTSLDkilKQEQLSVSELEq--aGRYVQ 766 Marinomonas sp....
Feature 1           
YP_001268056 724 DVY 726 Pseudomonas putida F1
YP_001418281 730 DVY 732 Xanthobacter autotrophicus Py2
YP_001768532 723 DVY 725 Methylobacterium sp. 4-46
EDU12822     777 DVY 779 Burkholderia pseudomallei 1655
EDT29252     734 DVY 736 Oligotropha carboxidovorans OM5
ZP_01546235  737 DVY 739 Labrenzia aggregata IAM 12614
YP_988268    723 DLF 725 Acidovorax sp. JS42
YP_941737    738 DVY 740 Psychromonas ingrahamii 37
YP_767868    730 DVY 732 Rhizobium leguminosarum bv. viciae 3841
ZP_01076314  767 DVY 769 Marinomonas sp. MED121

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