1KRH,1TVC,2R6H,1QFJ


Conserved Protein Domain Family
O2ase_reductase_like

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cd06187: O2ase_reductase_like 
Click on image for an interactive view with Cn3D
The oxygenase reductase FAD/NADH binding domain acts as part of the multi-component bacterial oxygenases which oxidize hydrocarbons using oxygen as the oxidant. Electron transfer is from NADH via FAD (in the oxygenase reductase) and an [2FE-2S] ferredoxin center (fused to the FAD/NADH domain and/or discrete) to the oxygenase. Dioxygenases add both atoms of oxygen to the substrate, while mono-oxygenases (aka mixed oxygenases) add one atom to the substrate and one atom to water. In dioxygenases, Class I enzymes are 2 component, containing a reductase with Rieske type [2Fe-2S] redox centers and an oxygenase. Class II are 3 component, having discrete flavin and ferredoxin proteins and an oxygenase. Class III have 2 [2Fe-2S] centers, one fused to the flavin domain and the other separate.
Statistics
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PSSM-Id: 99784
View PSSM: cd06187
Aligned: 32 rows
Threshold Bit Score: 205.905
Threshold Setting Gi: 158430640
Created: 21-Feb-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1KRH: Acinetobacter sp. Benzoate dioxygenase reductase binds FAD, contacts determined at 4A
    View structure with Cn3D
  • Structure:1TVC: Methylococcus capsulatus Methane monooxygenase binds dihydroflavin-adenine dinucleotide, contacts determined at 4A
    View structure with Cn3D
  • Comment:contacts lie primarily in the N-terminal region

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                       #           ####               ### #   ###       
1KRH_A       113 LARVENLSdsTITFDIQLddgqpdihFLAGQYVNVTLPGttetRSYSFSSQPgn---rLTGFVVRNVpQGKMSEYLSvqa 189 Acinetobacter sp.
YP_933354    109 VSRIESLTptIKGVWVKLdepe-gmrFQAGQYINLHLPDgigtRAFSIASAPsa--agEIELNIRIVaGGQGTAYVHekl 185 Azoarcus sp. BH72
AAC45296     119 IVATEPLThdILKVTIQTdrp---vhYLAGRYANVRVPGwprfRCYSFANAPqrkgrnVLEFYIRKVpAGEFTEALFrge 195 Pseudomonas chl...
AAD12606     103 VTAIDEAThdIRRLRIKLakp---leFSPGQYATVQFTPec-vRPYSMAGLPsd---aEMEFQIRAVpGGHVSNYVFnel 175 Ralstonia sp. U2
Q52126       103 VVAVESPThdIRRLRVRLskp---feFSPGQYATLQFSPeh-aRPYSMAGLPdd---qEMEFHIRKVpGGRVTEYVFehv 175 Pseudomonas putida
YP_001341516 102 IVAYDVLSndVRSLKLKInkg---feYSPGQFANLTFWRedgtRSYSMAGVTed---dLLEFHIRLVpNGRVTSKLDehi 175 Marinomonas sp....
YP_118439    110 VVSLDPIArdTRRLVLEVdga---ldFLPGQYAQLHVPGtecwRPYSMANSPar--phRLEFHIRYSdGGVATGWIFhgl 184 Nocardia farcin...
YP_001372583 108 VIGLYDLThdIKLIRIAPadgs-slpFTAGQYAQIRFTGtp-vRSYSMANRVge---gSLEFHVRRVaGGITSEHIHgal 182 Ochrobactrum an...
NP_354213    109 VEEAVDAThdIKLIRIRLedra-qftFKPGQYVRLLYPDss-pRDYSIASRVde---eLIEFHIRHVpGGMTSGRIFsla 183 Agrobacterium t...
AAQ19820     109 VVTLEALThdIRRVRIRLadeq-plmFFAGQYADIKFGQap-aRSYCMANRPge---aELEFHIRRVpGGVASAYVHtvl 183 Alcaligenes fae...
Feature 1                                       #  #                                             
1KRH_A       190 KAGDKMSFTGPFGSFYLRdv-kRPVLMLAGGTGIAPFLSMLQVLEQkgseHPVRLVFGVTQdcDLVALEQLDALqqk-lp 267 Acinetobacter sp.
YP_933354    186 KAGDAIRITGPYGRFFVKksakVPVVFMAGGSGLSSPRSMILDLLEegfdLPITLVYGQRSraELYYHDEFLALaqr-hp 264 Azoarcus sp. BH72
AAC45296     196 LDGQPLEMEAPQGTFHLHgg-dAPMLCIAGGSGLAPLVSILEHARAnrikRDCILLFGARTegDLYQLEAIGNIaanwqg 274 Pseudomonas chl...
AAD12606     176 SVGASVRISGPLGTAYLRrthtGPMLCVGGGTGLAPVLSIVRGALEsgmsNPIHLYFGVRSeqDIYDEERLHALaar-fp 254 Ralstonia sp. U2
Q52126       176 REGTSIKLSGPLGTAYLRqkhtGPMLCVGGGTGLAPVLSIVRGALKsgmtNPILLYFGVRSqqDLYDAERLHKLaad-hp 254 Pseudomonas putida
YP_001341516 176 EIGASIKLNGPLGASYLRrknnDPMLCVATGTGLAPILSIVRGALEsgmkNDIHLVFGARTeeDLYGLGYLDHLate-yt 254 Marinomonas sp....
YP_118439    185 TVGDRVELRGPLGDFVLDda-dHPLLLLAGGTGLAPLSAMLRAALErghrSPIHLYHGVREeaDLYDVEWLSELsrs-hp 262 Nocardia farcin...
YP_001372583 183 KSGDKVELEFPLGSSYLRqnhsGPMLCIAGGSGLAPIKSIVETALAhgmkQPIHVYFGARSecDLYLVEHFQSLaeq-ya 261 Ochrobactrum an...
NP_354213    184 RAGDPVTIVGPFGSSFLRekhcGPILGIAGGSGLAPVKAVVEAALAtgreRPVHVYFGARAerDLYMLDRFQDLttr-hg 262 Agrobacterium t...
AAQ19820     184 EPGERVTLELPKGTSYLReghgGPMLCIAGGAGLAPIKSIVETALAsgmsQAIHVYFGVREgrDLYGLEEFRALard-yp 262 Alcaligenes fae...
Feature 1                                                                             # #
1KRH_A       268 WFEYRTVVAHaes---qheRKGYVTGHIeydwl-nggevDVYLCGPVPMVEAVRSWLDTQGiqpaNFLFEKF 335 Acinetobacter sp.
YP_933354    265 NFRYVPALSHepegsdwngFRGFVHEAAkaafdndfrghKAYLCGPPLMIDACITTLMQGRlferDIYMEKF 336 Azoarcus sp. BH72
AAC45296     275 EFRFIPVLSHepehsdwtgARGLVTEHIpadf---cegaEGYLCGPPPMIDAAIARLLDNRlpleKIFYDKF 343 Pseudomonas chlororaphis
AAD12606     255 NLKVNVVVATgpag--pgrRSGLVTDLIgrdlp-nlagwRAYLCGAPAMVEALNLLVARLGivpgHIHADAF 323 Ralstonia sp. U2
Q52126       255 QLTVHTVIATgpin--egqRAGLITDVIekdil-slagwRAYLCGAPAMVEALCTVTKHLGispeHIYADAF 323 Pseudomonas putida
YP_001341516 255 NFQYLIALDHanpn--stsFRGLVTDAIaahfp-elknwRIYLAGAPAMVEAASLACTKRGadieRIYADAF 323 Marinomonas sp. MWYL1
YP_118439    263 DFHYTPCLSRgew----ngRTGYVGDAVladfd-nlrdySGYLCGPPAMVEAAGRALKRRRmaprRIFREKF 329 Nocardia farcinica IFM ...
YP_001372583 262 NLTFVVVLSEaea---tqyRCGFVTKAVaddlm-dldgwKAYVAGPPGMVDAAIQLALARGlrgeDMHADVF 329 Ochrobactrum anthropi A...
NP_354213    263 NLSFVPVLSNedh---aniRRGYVGAAVaddfd-dldgwKAYIAGPPAMIEATVPQLLARGmrtaDIHADVF 330 Agrobacterium tumefacie...
AAQ19820     263 TLLFTPVVSGipv---ahcSRGLVTDIAgseqa-dlrgwKVYVAGSPTLVDATIGMALKRGlkiqDLHAQIY 330 Alcaligenes faecalis

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