1IB0,1CNF,1UMK,2EIX


Conserved Protein Domain Family
cyt_b5_reduct_like

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cd06183: cyt_b5_reduct_like 
Click on image for an interactive view with Cn3D
Cytochrome b5 reductase catalyzes the reduction of 2 molecules of cytochrome b5 using NADH as an electron donor. Like ferredoxin reductases, these proteins have an N-terminal FAD binding subdomain and a C-terminal NADH binding subdomain, separated by a cleft, which accepts FAD. The NADH-binding moiety interacts with part of the FAD and resembles a Rossmann fold. However, NAD is bound differently than in canonical Rossmann fold proteins. Nitrate reductases, flavoproteins similar to pyridine nucleotide cytochrome reductases, catalyze the reduction of nitrate to nitrite. The enzyme can be divided into three functional fragments that bind the cofactors molybdopterin, heme-iron, and FAD/NADH.
Statistics
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PSSM-Id: 99780
View PSSM: cd06183
Aligned: 142 rows
Threshold Bit Score: 114.586
Threshold Setting Gi: 46095632
Created: 31-Jul-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:IB0: Rat cytochrome b5 reductase binds FAD, contacts determined at 4A
    View structure with Cn3D
  • Structure:1CNF: Zea mays Cytochrome B Reductase binds FAD, contacts determined at 3.5A
    View structure with Cn3D
  • Citation:PMID 7812715
  • Comment:FAD contacts primarily in the N-terminal region
  • Citation:PMID 7893687

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                        #              ##
1IB0_A         18 LRLIDKEILs---------------------hDTRRFRFALPsp---------qhILGLPIGQHIYLStrid-gnlVIRP 66   Norway rat
EAU84518       77 ATVVSNLDSg---------------------pDTKLLRLALPpqvaa---faaqdPSSFKAVWSVFIKddd---iqVERP 129  Coprinopsis c...
EAS07491        5 TTFVSVSTYyqnnl--------------nelqNEIFLKTVLDnp---------dqHLGTKVGQAVKLTlnsqkqnqISRY 61   Tetrahymena t...
EAK80865      126 FPLISSSYYpepd----------------rasVHKLLHIGVPasvvgsdvrnedkLKQLLRVRSIYIKeps---lvIERA 186  Ustilago mayd...
XP_001644643   64 YRVSYKKPI-----------------------DGSHFLMELTpvvkqr--tnlwlKLESEKLWSVEVKqpe---imVARN 115  Vanderwaltozy...
XP_566575      81 HKLVTIKIPsss-----------------ygfFDKPYRLDGEva---------dvKGGEVVIQHVMVKspd---iqIERP 131  Cryptococcus ...
CAB11221       45 FTVNKITELt---------------------sDASLFSLVPQsps--------ehLTTLEPIAKVTIRdps---mqVQRP 92   fission yeast
XP_001468899  942 VPVVVRESRasdq----------------fgvGSRVLRFNLPga---------tqTSGLTVGEFIGIRgdwd-gqqLIGY 995  Leishmania in...
XP_001020236   37 QFLIKKQISp----------------------TSSIYRFKMNde---------rkVLGIQLGENIQIGvpgnqqenHIAK 85   Tetrahymena t...
AAX20162      963 ATILQKREIalsktswtsvvvresrsgeqfgtGSRVLRFNLPga---------lqRTGLNLGEFVAIRgewd-gqqLVGY 1032 Trypanosoma b...
Feature 1         ##                            ### #          #####                              
1IB0_A         67 YTPVSSDDDKg---------------fVDLVVKVYfkethpkfpaGGKMSQYLENMn---IGDTIEFRGPn--------- 119  Norway rat
EAU84518      130 YTPLEGIDENg---------------eMVFWIKKYp---------RGEVGRWLHTKa---AGDVIELRGPl--------- 173  Coprinopsis c...
EAS07491       62 YSLVSDPFSKg---------------yFDCIVKDLdksn--yaenSNPMVPQLLQLk---DGAKVVIEDPy--------- 112  Tetrahymena t...
EAK80865      187 YTPLYDTLPGsysmalvg-asaeekqrVDLLVKRYp---------DGELGKMLHRAvpnpAVPQIEVRGPvdt-----ws 251  Ustilago mayd...
XP_001644643  116 YTPLPLKYDAqsddivplkdgdnaggkLFFYLKQYp---------DGEVSRWLFKMk---EGDQVELRGPi--------- 174  Vanderwaltozy...
XP_566575     132 YTLINDPNAAnge------------reMRMVVKRVr---------GGEVGRVVHTTk---EGEMLGVRGPi--------- 178  Cryptococcus ...
CAB11221       93 YTPLYLDANE-----------------LKFFIRKYe---------EGPVSSYIHSKk---EGDTIELRGP---------- 133  fission yeast
XP_001468899  996 YSPINMPDDKg---------------rISILARGD----------KGNLQEWISSMr---PGDSVEMKACg--------g 1039 Leishmania in...
XP_001020236   86 VHPLTRIDDPa---------------iVDVLVDEQ----------IPEQQFFSNLK----EKQEVVIKSDq--------n 128  Tetrahymena t...
AAX20162     1033 FSPITLPEDLg---------------tISLLVRAD----------KGTLKEWICALr---PGDSVEIKACgglridqdpv 1084 Trypanosoma b...
Feature 1                                                       #  ##                             
1IB0_A        120 GLLVYQGKGkfairadkk-----------snpvvrtvKSVGMIAGGTGITPMLQVIRAVLkdp-----ndhTVCYLLFAN 183  Norway rat
EAU84518      174 TTWPWKDDTw---------------------------DEVVMISGGTGFTPFYQLFHSIIsnps---lspsTQFTFLHSS 223  Coprinopsis c...
EAS07491      113 TQYIYHGLGnltfkkkqivqs-----gfnsefktgrfDKIGIIAHNSAITTFSQLIQGVIsar------dpTQINLLFHN 181  Tetrahymena t...
EAK80865      252 FERDSAASGei-------------------------pDRIVMIVGGTGITPAYQLLTNLFgrpctsatstaDKLSVSLFS 306  Ustilago mayd...
XP_001644643  175 TEYELPRDSderkrdrgflfdsktiknhdvetfrwrpFDISFYAAGTGIAPALQLTLSEDpf--------kGKLQLFYSC 246  Vanderwaltozy...
XP_566575     179 PTFSIYPQRy---------------------------DKIIMISTGTAVSPFLQLLAKLSps-------naPRLELIQAL 224  Cryptococcus ...
CAB11221      134 FKTTKLDCTk--------------------------yPRIVAIVAGTGIAPIYQLAQSVK-----------SPVDIVYCS 176  fission yeast
XP_001468899 1040 LRIELKPHQkqmvy------------------rktviRKLGLIAGGSGVAPMLQIIKAALnrpy---vdsiETIRLVYAA 1098 Leishmania in...
XP_001020236  129 KKFVYDGFGkikifnqg-------------qvtqkkvDYLGIIVQGYHISKVFSLIEGIStng------dkTNISILYVN 189  Tetrahymena t...
AAX20162     1085 KKCLLFRNRp--------------------------iTRFALVAAGTGVAPMLQVIRAALkkpy---vdtlESIRLIYAA 1135 Trypanosoma b...
Feature 1                                                                                         
1IB0_A        184 QSEkd----------iLLRPELEELRneh----------------ssRFKLWYTVDKapdaw-----------dysqGFV 226  Norway rat
EAU84518      224 RTPeelp------pasLLNPLITFAQen-----------------paRLKVNLFVDQneskss--------gydlsvTRI 272  Coprinopsis c...
EAS07491      182 HKIqd----------eILLDEMTRFFls------------------hNMKYSTVLQNiiy--------------qpsKFI 219  Tetrahymena t...
EAK80865      307 EKLagspsalspaelsALGPQLTASSsgrswlsslw--gksrsaldpQLRLTSSQSPttti------------pvyeSRI 372  Ustilago mayd...
XP_001644643  247 KQLee---------lgPLAKYLELLQqn------------------gRIELNLYESSkgnslrkdtkklldtipapsKYV 299  Vanderwaltozy...
XP_566575     225 PALsss-------tppFSHESLDRAApikwdwvdtnedpsflpslekKFGEKLKITRfpqg------------avpaGAV 285  Cryptococcus ...
CAB11221      177 RPGqp----------pLLKEELEKECp--------------------NVRVKSVQNRlvn---------------ihDIL 211  fission yeast
XP_001468899 1099 EDEye----------lTYRSLLKQYRadn----------------pdKFDCGFVLNNppegw-----------tegvGYV 1141 Leishmania in...
XP_001020236  190 SNLde----------sLFIDELTWYAe--------------------EKKIHLGVLFekvpdgvp----vmkgkfqkHHV 235  Tetrahymena t...
AAX20162     1136 EEYdt----------lTYRSILQRFAeef----------------pdKFVCNFVLNNppegw-----------tggvGFV 1178 Trypanosoma b...
Feature 1                                                                                         
1IB0_A        227 NEEMIrdhlpp-----------------pgeetlILMCGPPPMIq-------------fACLPNLERvgh---------- 266  Norway rat
EAU84518      273 NADVIkrsigldkegkswwnwlygkkpeapkrrlFLVCGPDPMVaaiagpf-grnmsqgQLGGALSKlgf---------- 341  Coprinopsis c...
EAS07491      220 QKKAKsdyygfngdvcers--iiqtlpsprenyvIMLQGPRPFKe--------------KVYQLLLKmg----------- 272  Tetrahymena t...
EAK80865      373 VQQHIeqllspikp------------qqktgrtlILVSGPDGMVeaiagp----ksrdgQHQGSLSVssklsvytpaffr 436  Ustilago mayd...
XP_001644643  300 PKATFksnsnas----------------iispvlSLVCGPDEYIstiagpk--ylqaqgQTGGLLKQkgw---------- 351  Vanderwaltozy...
XP_566575     286 QAALEgmnr---------------------ervlVLVCLPPWLMrplcggm-trnldqgPVTGVLRElgl---------- 333  Cryptococcus ...
CAB11221      212 DWDNVtvpl---------------------kdtlCIVCGSQKFVstiagpkadygarqgEVKGLLSNnp----------- 259  fission yeast
XP_001468899 1142 DRATLqsllpp-----------------pskgllVAICGPPVMQr--------------SVVADLLAlgy---------- 1180 Leishmania in...
XP_001020236  236 SDFMPpvdq----------------------efhLIVAGSNTFQd--------------ECLGHLKSlgy---------- 269  Tetrahymena t...
AAX20162     1179 NKKSLqkvlqp-----------------psseplIVVCGPPVMQr--------------DVKNELLSmgy---------- 1217 Trypanosoma b...
Feature 1                #
1IB0_A        267 pkERCFTF 274  Norway rat
EAU84518      342 ksQDVYKL 349  Coprinopsis cinerea okayama7#130
EAS07491      273 ydSERIAY 280  Tetrahymena thermophila SB210
EAK80865      437 lpSRRTLF 444  Ustilago maydis 521
XP_001644643  352 neENVYKL 359  Vanderwaltozyma polyspora DSM 70294
XP_566575     334 gsKQVYKL 341  Cryptococcus neoformans var. neoformans JEC21
CAB11221      260 -fGKVWKL 266  fission yeast
XP_001468899 1181 naEMVRTV 1188 Leishmania infantum JPCM5
XP_001020236  270 knENITLY 277  Tetrahymena thermophila SB210
AAX20162     1218 dkELVHTV 1225 Trypanosoma brucei

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