1AMO,1DDG,1GAQ,1TLL,2BF4,2QTL


Conserved Protein Domain Family
CYPOR_like
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cd06182: CYPOR_like 
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NADPH cytochrome p450 reductase (CYPOR) serves as an electron donor in several oxygenase systems and is a component of nitric oxide synthases and methionine synthase reductases. CYPOR transfers two electrons from NADPH to the heme of cytochrome p450 via FAD and FMN. CYPOR has a C-terminal ferredoxin reducatase (FNR)- like FAD and NAD binding module, an FMN-binding domain, and an additional conecting domain (inserted within the FAD binding region) that orients the FNR and FMN binding domains. Ferredoxin-NADP+ (oxido)reductase is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin. Isoforms of these flavoproteins (i.e. having a non-covalently bound FAD as a prosthetic group) are present in chloroplasts, mitochondria, and bacteria and participate in a wide variety of redox metabolic pathways. The C-terminal domain contains most of the NADP(H) binding residues and the N-terminal domain interacts non-covalently with the isoalloxazine rings of the flavin molecule which lies largely in a large gap betweed the two domains. Ferredoxin-NADP+ reductase first accepts one electron from reduced ferredoxin to form a flavin semiquinone intermediate. The enzyme then accepts a second electron to form FADH2, which then transfers two electrons and a proton to NADP+ to form NADPH.
Links
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Statistics
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PSSM-Id: 99779
Aligned: 15 rows
Threshold Bit Score: 241.087
Created: 12-May-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:FAD binding pocket [chemical binding site]
Evidence:
  • Structure:1DDG: E. coli sulfite reductase binds FAD, contacts determined at 3.5A
  • Structure:1TLL: Rat NO synthase reductase binds FAD, contacts determined at 3.5A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                    #    
1AMO_A        222 AVTANRKLNQgte----------------------rhLMHLELDisd-------------------skIRYESGDHVAVY 260  Norway rat
1DDG_A         15 SLSVNQKITGrns---------------------ekdVRHIEIDlgd-------------------sgLRYQPGDALGVW 54   Escherichia coli
1GAQ_A         41 RCLLNTKITGdda---------------------pgeTWHMVFSte--------------------gkIPYREGQSIGVI 79   maize
1TLL_A        255 RLLSRQNLQSpks---------------------srsTIFVRLHtng------------------nqeLQYQPGDHLGVF 295  Norway rat
2QTL_A        118 PISKAVQLTTnda---------------------iktTLLVELDisn-------------------tdFSYQPGDAFSVI 157  human
P14779        666 NVVASKELQQpgs---------------------arsTRHLEIElp--------------------keASYQEGDHLGVI 704  Bacillus mega...
AAC06216      511 WSLARRECLNpgsq--------------------gesTWLLDLRapa------------------nsaIQWRAGDLLEIV 552  Pseudomonas a...
AAK27387      286 SSASSTSTTPspd---------------------pekVRLLEACassetgpqll-----rslsksstpLCYPSLVDVLEV 339  Leishmania do...
CAL57731      674 AITGNGARMN---------------------------TVWLKFRliha----------------reevQYYKPGDHVAVW 710  Ostreococcus ...
YP_001268056  451 LETVNRQCPQafarwghalgdvlgqalalvhtpqqprTHSLELTeriaygeqvnapthvlrfnargglPNFLAGDLVGIL 530  Pseudomonas p...

Feature 1                                                                                         
1AMO_A        261 PANdsalvnqigeilgadldvimslnnldeesnkkhpfpcpttyrtaltyylditnpprtnvlye------laqyaseps 334  Norway rat
1DDG_A         55 YQNdpalvkelvellwlkgdepvtvegktlplnealqwhfeltvntaniveny--------------------------- 107  Escherichia coli
1GAQ_A         80 ADGvdk-------------------------------------------------------------------------- 85   maize
1TLL_A        296 PGNhedlvnalierledappanhvvkvemleerntalgvisnwkdesrlppctifqafkyyldittpptplqlqqfasla 375  Norway rat
2QTL_A        158 CPNsdsevqsllqrlqledkrehxvllkikadtkkkgatlpqhipagxslqfiftwcleiraipkka--flralvdytsd 235  human
P14779        705 PRNyegivnrvtarfgldasqqirleaeeeklahlplaktvsveellqyvelqdpvtrtql-------------ramaak 771  Bacillus mega...
AAC06216      553 PHQapvrirewlqrhnldgqarvavegveqsl------------------------------------------------ 584  Pseudomonas a...
AAK27387      340 FSFvqipldr---------------------------------------------------------------------- 349  Leishmania do...
CAL57731      711 PQTseararyfaahfgltfrdvlelvpksdallvkksidpaipnvvsvehlftrvldingeasa--------allsalah 782  Ostreococcus ...
YP_001268056  531 PPG----------------------------------------------------------------------------- 533  Pseudomonas p...

Feature 1                                                                 ####                 # #
1AMO_A        335 eqehlhkmasssgegkelylswvvearrhilailqdypslrppidhlcellpRLQARYYSIASSSkvh---pnsVHICAV 411  Norway rat
1DDG_A        108 ---atltrsetllplvgdkaklqhyaattpivdmvrfspaqldaealinllrPLTPRLYSIASSQaev---eneVHVTVG 181  Escherichia coli
1GAQ_A         86 -------------------------------------------------ngkPHKVRLYSIASSAigdfgdsktVSLCVK 116  maize
1TLL_A        376 tnekekqrllvlskglqeyeewkwgknptmvevleefpsiqmpatllltqlsLLQPRYYSISSSPdmy---pdeVHLTVA 452  Norway rat
2QTL_A        236 saekrrlqelcskqgaadysrfvrdaxaclldlllafpscqpplslllehlpKLQPRPYSCASSSlfh---pgkLHFVFN 312  human
P14779        772 tvcpphkveleallekqaykeqvlakrltmlellekypacemkfsefiallpSIRPRYYSISSSPrvd---ekqASITVS 848  Bacillus mega...
AAC06216      585 -----------------------eqalagrllpdsfehlvglhpqalldaliPLSVRQYSIASLQsd-----gdLQLIVR 636  Pseudomonas a...
AAK27387      350 ----------------------------------------------llevsgPLQTRRYSLANWIpatl-ppspLQLCMR 382  Leishmania do...
CAL57731      783 yafnedarqdwaemtgarifsifehfptlstlhrgdkavgldmlrdiilkipKLRPRYYSVSSSPqhag--nnvFALTVG 860  Ostreococcus ...
YP_001268056  534 ----------------------------------------------------SPIPRFYSLASGSqd-----gvLEICVR 556  Pseudomonas p...

Feature 1                                                    ####                                 
1AMO_A        412 AVEYeaksg------------------------------rvnkGVATSWLRAKepag----------------------- 438  Norway rat
1DDG_A        182 VVRYdveg-------------------------------raraGGASSFLADRve------------------------- 205  Escherichia coli
1GAQ_A        117 RLIYtndag------------------------------eivkGVCSNFLCDLq-------------------------- 140  maize
1TLL_A        453 IVSYhtrdge----------------------------gpvhhGVCSSWLNRIq-------------------------- 478  Norway rat
2QTL_A        313 IVEFlstatt----------------------------evlrkGVCTGWLALLvasvlqpnih------------ashed 352  human
P14779        849 VVSGeawsg-----------------------------ygeykGIASNYLAELq-------------------------- 873  Bacillus mega...
AAC06216      637 QEQHadg----------------------------------slGICSGWLTEYlp------------------------- 657  Pseudomonas a...
AAK27387      383 EVCArrsanlpaatavgadaqrvadmlnraaqdasrdhsdfffGHTSHPLCCAarsmtrsaraagqrgmyvsfslfgnsl 462  Leishmania do...
CAL57731      861 RVTYksgag-----------------------------armhlGFCSDFLATLp-------------------------- 885  Ostreococcus ...
YP_001268056  557 KHSG---------------------------------------GMCSEFLHGLd-------------------------- 571  Pseudomonas p...

Feature 1                                                                                         
1AMO_A        439 -enggRALVPMFVRKs-QFRLPfk-stTPVIMVGPGTGIAPFMGFIQERawlr---eqgkEVGETLLYYGCRRsdEDYLY 512  Norway rat
1DDG_A        206 ----eEGEVRVFIEHndNFRLPan-peTPVIMIGPGTGIAPFRAFMQQRaa-------deAPGKNWLFFGNPHftEDFLY 273  Escherichia coli
1GAQ_A        141 ----pGDNVQITGPVgkEMLMPkd-pnATIIMLATGTGIAPFRSFLWKMffekh--ddykFNGLGWLFLGVPTs-SSLLY 212  maize
1TLL_A        479 ----aDDVVPCFVRGapSFHLPrn-pqVPCILVGPGTGIAPFRSFWQQRqfdiq--hkgmNPCPMVLVFGCRQskIDHIY 551  Norway rat
2QTL_A        353 sgkalAPKISIFPRTtnSFHLPdd-psIPIIMVGPGTGIAPFIGFLQHReklqe-qhpdgNFGAMWLFFGCRHkdRDYLF 430  human
P14779        874 ----eGDTITCFISTpqSEFTLpkdpeTPLIMVGPGTGVAPFRGFVQARkqlk---eqgqSLGEAHLYFGCRSphEDYLY 946  Bacillus mega...
AAC06216      658 ----lGAALTLRLRRnaGFHLPed--dVPLILIGNGTGLAGLRSLLRASia--------aGRKRNWLLFGERNfaHDYYC 723  Pseudomonas a...
AAK27387      463 farqlQAGCTALCNPaqAKSLC-----SQLFLIGCGTGIAPLIAAVTQLmlrrastaagsAPFPCWVFYGARTk-AELLY 536  Leishmania do...
CAL57731      886 ----iGANVTVEFRPapSFRLPrs-pqAPIIMVAGGTGIAPFKGFVEHFatl-----npgERGEAWLIAGCRSq-DNQLY 954  Ostreococcus ...
YP_001268056  572 ----iGAQIDAFIQPnpQFRPAsg--tHPVILIGAGTGIGPLAGFIRNNk----------ARHPMHLYWGGRNpaSDFLY 635  Pseudomonas p...

Feature 1                                                                                         
1AMO_A        513 REELARfhk-dgaLTQLNVAFSREQah------kVYVQHLLKrd-rEHLWKLIHEGgaHIYVCGDAr---nMAKDVQNTF 581  Norway rat
1DDG_A        274 QVEWQRyvk-egvLTRIDLAWSRDQke------kVYVQDKLReq-gAELWRWINDGa-HIYVCGDAn---rMAKDVEQAL 341  Escherichia coli
1GAQ_A        213 KEEFGKmkerapeNFRVDYAVSREQtnaa--gerMYIQTRMAey-kEELWELLKKDntYVYMCGLKg----MEKGIDDIM 285  maize
1TLL_A        552 REETLQakn-kgvFRELYTAYSREPdr-----pkKYVQDVLQeqlaESVYRALKEQggHIYVCGDVt----MAADVLKAI 621  Norway rat
2QTL_A        431 RKELRHflk-hgiLTHLKVSFSRDApvgeeeapaKYVQDNIQlh-gQQVARILLQEngHIYVCGDAk---nMAKDVHDAL 505  human
P14779        947 QEELENaqs--egIITLHTAFSRMPnq-----pkTYVQHVMEqd-gKKLIELLDQGa-HFYICGDGs---qMAPAVEATL 1014 Bacillus mega...
AAC06216      724 RAELEQala-rgeLQRLDLAFSRDQae------kIYVQDRLHea-aDELRRWIDEGa-ALYVCGSLq---gMAGGVDRVL 791  Pseudomonas a...
AAK27387      537 DETLQEalr-tgaIAKYEYALSREEdnk---kqgRYVTDLVKrn-rLMVTGSLQNEg-QLFVCGPAkallsVRQLVKCDL 610  Leishmania do...
CAL57731      955 ATEFNEavh-lghLTKYLVGFSRQPgv-----pkTYVDTVLReh-aDDLMDLISRGa-HVYVCGDVrietsVRGAMLDIL 1026 Ostreococcus ...
YP_001268056  636 EPELNQyls-drrLTQLHAAFSQVQe-------rSYVQDRLIsd-aLALRRLIEKGa-QVLVCGSRe----MAKGVMQAL 701  Pseudomonas p...

Feature 1                                            
1AMO_A        582 ydivaefgpmehtqaVDYVKKLmt--kgRYSLDVW 614  Norway rat
1DDG_A        342 leviaefggmdteaaDEFLSELrv--erRYQRDVY 374  Escherichia coli
1GAQ_A        286 vslaek----dgidwFDYKKQLkr--gdQWNVEVY 314  maize
1TLL_A        622 qrimtqqgklseedaGVFISRLrd--dnRYHEDIF 654  Norway rat
2QTL_A        506 vqiiskevgvekleaMKTLATLke--ekRYLQDIW 538  human
P14779       1015 mksyadvhqvseadaRLWLQQLee--kgRYAKDVW 1047 Bacillus megaterium
AAC06216      792 rev----------lgEEALQRLad--egRYRRDVY 814  Pseudomonas aeruginosa
AAK27387      611 laepdd----ddsvqEQRLLMLed--rgRLNFDIW 639  Leishmania donovani
CAL57731     1027 g-------------gEAAFNELek---sRYHLDIF 1045 Ostreococcus tauri
YP_001268056  702 dev----------laPLHLSVLilkaqgRYREDVY 726  Pseudomonas putida F1

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