2QXF,1FXX


Conserved Protein Domain Family
ExoI_N

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cd06138: ExoI_N 
Click on image for an interactive view with Cn3D
N-terminal DEDDh 3'-5' exonuclease domain of Escherichia coli exonuclease I and similar proteins
This subfamily is composed of the N-terminal domain of Escherichia coli exonuclease I (ExoI) and similar proteins. ExoI is a monomeric enzyme that hydrolyzes single stranded DNA in the 3' to 5' direction. It plays a role in DNA recombination and repair. It primarily functions in repairing frameshift mutations. The N-terminal domain of ExoI is a DEDDh-type DnaQ-like 3'-5 exonuclease containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The ExoI structure is unique among DnaQ family enzymes in that there is a large distance between the two metal ions required for catalysis and the catalytic histidine is oriented away from the active site.
Statistics
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PSSM-Id: 99841
View PSSM: cd06138
Aligned: 21 rows
Threshold Bit Score: 245.251
Threshold Setting Gi: 91762381
Created: 18-Mar-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #### ##                                            ##                        
2QXF_A       12 LFHDYETFGTHPALDRPAQFAAIRTDsEFNVigEPEVFYCKPADDYLPQPGAVLITGITPQEARAk-GENEAAFAARIHS 90  Escherichia coli...
CAD16834     18 FWHDYETFGAVPRRDRPAQFAGIRTDaELNEigEPVELFCQPSNDWLPDPMSCLITGITPQQCRRq-GIPEHRFAQAIER 96  Ralstonia solana...
NP_641931     6 LFYDLETFGQDPRRTRIAQFAAVRTDaQLRVieEPISFFVQPADDLLPSPYATMVTGITPQHALRe-GVTEAEAFARIAE 84  Xanthomonas axon...
ZP_01811085  10 FFYDLETSGLDPRRDRIMQFAGQRTTmDLDPigEPFNILVRLNDDVLPSPDAVMVTGITPQKTHEe-GIAEAEFIKLFHN 88  candidate divisi...
EDN37951      8 LFYDLETSGINNSFDQVLQFAAIRTDlDFNEi-ERFNFFVKLNPDTTPSPMATITHHISIAQANT--GIAEYQAIRKIHK 84  Francisella tula...
ZP_01058460   4 VFYDLETTGISPEFDQPLQFAAIWTDeNFVEk-DRVNIRCRLAPHILPSPQALVVTRVTPDQLIDpsLPSLFEFSQQVAE 82  Roseobacter sp. ...
ZP_01692729  24 VFYDTRSSGDNPVFDQILRADTYVTDhAFNLl-ETHHIEIKKRPDVVPSPEALIANNWLPSDLAK--GSTEYEAMRHIHE 100 Microscilla mari...
ZP_01116440  12 LWYDYETWGTVPSRDRIAQFAAVRTDmDLNIieEPINLFCKPDIDTVIDPGAVQITRLSPLELEQt-GLSEWEFSQAVHN 90  Reinekea sp. MED297
ZP_02001281   6 LFYDIETTGLNKTFAQILQFAAIRTDmQFNEi-ERHDILIKLRPDVIPSPKATITHRISIAQSMQ--GLCEFEAIMQIHR 82  Beggiatoa sp. PS
NP_820352     9 LFYDLETTGLNKCFDQIIQFAAIRTDeALNEl-NRHEILIRLNPDVIPSPEAMIIHRFSIDQMLS--GKAEIEAIREIHQ 85  Coxiella burneti...
Feature 1                   ##  ###                                                             
2QXF_A       91 LFTV-PKTCILGYNNVRFDDEVTRNIFYRNFYdPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPEND----DGLPSfr 165 Escherichia coli...
CAD16834     97 ELAT-PGTIGVGYNSIRFDDEITRHLFWRNLIdPYAREWQNTCGRWDLLDVVRTTWALRPDGVEWPKNG----DGKPSfk 171 Ralstonia solana...
NP_641931    85 QMGR-PQTCTLGYNSIRFDDEFIRCGLFRNFYdPYEREWRGGNSRWDLLDVLRLVHALRPDGIVWPQRE----DGATSfk 159 Xanthomonas axon...
ZP_01811085  89 QVCT-PGTVTVGFNNIRFDDEFIRFALWRNFHdPYEWAWKDGRSRWDLLDVVRMTRALRPGGIVWPVDK----SDKPTnr 163 candidate divisi...
EDN37951     85 IINA-PGTISIGYNTLGFDDEFLRFAFYKNMLpPYSHQFKNGCSRADLFPIVTCYYLFCNEVLNWPKAIdedgQEKTSlk 163 Francisella tula...
ZP_01058460  83 FTERwAPAIWVGYNTMKFDEEVLRQTFYQNLSpNIYATQFNGNTRFDILPAVYAAYVRHPGLLVWPTDD----TGRRSfk 158 Roseobacter sp. ...
ZP_01692729 101 LFNT-PHSYNVGYGNLNNDDLLVRFGFYRNGLtPYTHQWQRSCRRVDLRTIVTLYYLQAPNALVFSKNA----AGKVSle 175 Microscilla mari...
ZP_01116440  91 AMAQ-PNTCTTGYNSIRFDDECSRFLFYRNLInPYEREWKNGNSRWDLLDVVRMTHALRPDGIEWPRRD----DGSPSfr 165 Reinekea sp. MED297
ZP_02001281  83 LMNQ-SGTISLGYNTSSFDDEFLRFCFHRNLLpPYTHQYKDGCRRSDLLPITIMYYLYKKEVLENWPEI----DGKPTmr 157 Beggiatoa sp. PS
NP_820352    86 LVNT-PGTISVGYNTLGFDDEFLRFSFYRNLLpPYTHQYDNFCGRMDLYPLTILYYLFKPNALNWPDPF----DLKLEn- 159 Coxiella burneti...
Feature 1                       #    #         
2QXF_A      166 LEHLTKANgieh-snaHDAMADVYATIAMAK 195 Escherichia coli K12
CAD16834    172 LEHLSQANglqh-eaaHDALSDVRATIALAR 201 Ralstonia solanacearum
NP_641931   160 LEHLADANdvre-gdaHEALSDVYATIGMAR 189 Xanthomonas axonopodis pv. citri str. 306
ZP_01811085 164 LELLTSENnleh-sqaHDALSDVYALIAITR 193 candidate division TM7 genomosp. GTL1
EDN37951    164 LENLNQNNslysggraHDAITDVIVTVELAK 194 Francisella tularensis subsp. novicida GA99-3548
ZP_01058460 159 LDRLAPANgfna-hnaHDALGDVEATIHIAR 188 Roseobacter sp. MED193
ZP_01692729 176 LDDIAQANgltvgkgaTNAQTIMELAKLLHN 206 Microscilla marina ATCC 23134
ZP_01116440 166 LEHLTAANglsh-anaHDAVSDVKATIALAQ 195 Reinekea sp. MED297
ZP_02001281 158 LEKLSEVNqlai-gqaHNAIVDVEATVELAR 187 Beggiatoa sp. PS
NP_820352   160 LSKANQLAeg----qaHNAMVDVEASVALAK 186 Coxiella burnetii RSA 493

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