2F96,2IS3


Conserved Protein Domain Family
RNaseT

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cd06134: RNaseT 
Click on image for an interactive view with Cn3D
DEDDh 3'-5' exonuclease domain of RNase T
RNase T is a DEDDh-type DnaQ-like 3'-5' exoribonuclease E implicated in the 3' maturation of small stable RNAs and 23srRNA, and in the end turnover of tRNA. It contains three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. RNase T is related to the proofreading domain of DNA polymerase III. Despite its important role, RNase T is mainly found only in gammaproteobacteria. It is speculated that it might have originated from DNA polymerase III at the time the gamma division of proteobacteria diverged from other bacteria. RNase T is a homodimer with the catalytic residues of one monomer contacting a large basic patch on the other monomer to form a functional active site.
Statistics
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PSSM-Id: 99837
View PSSM: cd06134
Aligned: 16 rows
Threshold Bit Score: 340.039
Threshold Setting Gi: 29654993
Created: 28-Feb-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                  #### #                                                                
2F96_A        25 RFRGYLPVVVDVETGGFNSATDALLEIAATTVGXDEKGFLFPEHTYFFRIEPFEGANIEPAALEFTGIK-LDHPLRXAVQ 103 Pseudomonas aer...
Q8PAG3        21 RFRGYLPVVVDVETGGFDWNKHALLEIACVPIEMDAQGHFFPGETASAHLVPAPGLEIDPKSLEITGIV-LDHPFRFAKQ 99  Xanthomonas cam...
YP_001209227   8 RFRGFLPVVVDVETGGFDHEKDALLEVAAVLVNFNEAGNLAPVETFHYHVKPFEGAHLNPDSLKINGID-PFHPLRPALD 86  Dichelobacter n...
ZP_01450509   10 RFRHYFPVIIDVETAGFNAQTDALLEIAAVTVRMDEAGMLHPDQEIHYHINPFEGANIEPAAIEFNGIK-VDCALRGAVD 88  alpha proteobac...
ZP_01999989   12 RFRGFLPVVVDVETAGFNPERDALLEIAAVILQINEEGLWQPHQTHFSHIVPFEGANLDKQALEFIGVD-PFHPFRFAKQ 90  Beggiatoa sp. PS
YP_114507     11 RFRSYLPVVVDVETSGFDPHANALLEIAAVHLDMDESGNLHPVRTHQLHVKPFEGAKLDEAALNFNKIN-PFHPFRFAVD 89  Methylococcus c...
NP_820685     14 RFSGYLPVVVDIETGGLEPLKNPLLEIAAVLVEVNKEGQLCQGELFACHVLPFEGAALDPASLEITRID-PYHPFRFAVD 92  Coxiella burnet...
YP_390867     10 RFRGFLPVVVDVETAGFDPQTNALIEIAIVTLKMNSDGTIERDQTVQANVLPFKGSQLDKSALKFIGVEdPTHPFRMAVS 89  Thiomicrospira ...
YP_096957     15 RFRGFLPVVVDVETAGIDPQKNALLEMCIVLLDMDEQGLLKKDSSYFEHILPFPGAELDQKSLEFNQID-PYQPLRFAVD 93  Legionella pneu...
ZP_01217165    8 RFRGYYPVIIDIETSGLNSQTDAILEIAAIFPAMDEQGYLYSAQTIHFNVQPFEGANMDPKALEFTGID-PFNPLRGAID 86  Psychromonas sp...
Feature 1                                    ## ###                                              
2F96_A       104 EEAALTEIFRGIRKALKaNGCKRAILVGHNSSFDLGFLNAAVARTGIkRNPFHPFSSFDTATLAGLAYGQtVLAKACQAA 183 Pseudomonas aer...
Q8PAG3       100 EKDALDHVFAPVRAAVKkYGCQRAILVGHNAHFDLNFLNAAVARVGHkRNPFHPFSVFDTVTLAGVAYGQtVLARAAQAA 179 Xanthomonas cam...
YP_001209227  87 EVTVAKQLFGAIREYQKaQSCTRSILVGHNAHFDLGFINALAARCNYqHNPFHPFSSLDTVSLGALAYGQtVLARIAKAA 166 Dichelobacter n...
ZP_01450509   89 ETEAMKGLSKAISKLQKaANCQRSVIVAHNASFDMSFVNAALARTNVkRSPFHPFVSFDTTSLAALTLGQtVLVKACEAA 168 alpha proteobac...
ZP_01999989   91 EAEGLETVFKPIRKAVKiYGCTRAVLVGHNPSFDLSFINAASERTGV-KSPFHRFSTFDTATLAGLVYGQtVLARAVQAA 169 Beggiatoa sp. PS
YP_114507     90 EREALSQIFDAVQQSIRqHGCTRAILVGHNPAFDIAFLNAASSRARIkKNPFHPFSTFDTATLGGLAYGQtVLARAAQAA 169 Methylococcus c...
NP_820685     93 EKKALQDLFAFVEKAVNvAQCRRAVLVGHNAHFDLTFIQAAMKRCKLkKSPFHAFTCFDTATLAAIVFGKtVLAKALRAA 172 Coxiella burnet...
YP_390867     90 EKDALEKCFAPIRQLLKeTDCSRAILVGHNAFFDLSFVKAAADRCKV-KSPFHEFSTFDTVSLAGLAYGQtVLAKAVQMA 168 Thiomicrospira ...
YP_096957     94 EQTALERLFKPIHDLLKkTRCQRAVLVGHNAWFDLLFIKAAIKRTGI-KSPFHAFTCFDTATLAGLIFGEtVLAKAAQAA 172 Legionella pneu...
ZP_01217165   87 EKEVLTEIFKLVRKHQKiNDCNRSILVAHNAAFDQGFINKASERCNFkRIPFHPFVCFDTASMAGLMLGQtVLAKACEQA 166 Psychromonas sp...
Feature 1                 #    #               
2F96_A       184 GXEFDNREAHSARYDTEKTAELFCGIVNRW 213 Pseudomonas aeruginosa
Q8PAG3       180 GLDWNSADAHSAVYDTEQTARLFCKIANAW 209 Xanthomonas campestris pv. campestris
YP_001209227 167 GFEYDSERAHGAKYDTELTAQIFCHIINTW 196 Dichelobacter nodosus VCS1703A
ZP_01450509  169 NIAFDQKQAHGALYDAQRTAELFCFMMNRY 198 alpha proteobacterium HTCC2255
ZP_01999989  170 GFEWNTSEAHAAIYDAQRTADLFCTIVNSW 199 Beggiatoa sp. PS
YP_114507    170 GLSWDHQEAHSAAYDANQTARLFCTIVNRW 199 Methylococcus capsulatus str. Bath
NP_820685    173 NLPFDKKEAHSAIYDAKCAAELFCKITNQI 202 Coxiella burnetii RSA 493
YP_390867    169 EIEWDNSKAHSAEYDTIKTADLFCKIVNQW 198 Thiomicrospira crunogena XCL-2
YP_096957    173 GLHFDSQEAHSAIYDAEKTADLFCLMINKW 202 Legionella pneumophila subsp. pneumophila str. Philadelphia 1
ZP_01217165  167 NIDYNNEEAHSALYDTQVTADLFFYMVNKL 196 Psychromonas sp. CNPT3

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