1W0H,1ZBH


Conserved Protein Domain Family
ERI-1_3'hExo_like

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cd06133: ERI-1_3'hExo_like 
Click on image for an interactive view with Cn3D
DEDDh 3'-5' exonuclease domain of Caenorhabditis elegans ERI-1, human 3' exonuclease, and similar proteins
This subfamily is composed of Caenorhabditis elegans ERI-1, human 3' exonuclease (3'hExo), Drosophila exonuclease snipper (snp), and similar proteins from eukaryotes and bacteria. These are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. ERI-1 has been implicated in the degradation of small interfering RNAs (RNAi). 3'hExo participates in the degradation of histone mRNAs. Snp is a non-essential exonuclease that efficiently degrades structured RNA and DNA substrates as long as there is a minimum of 2 nucleotides in the 3' overhang to initiate degradation. Snp is not a functional homolog of either ERI-1 or 3'hExo.
Statistics
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PSSM-Id: 99836
View PSSM: cd06133
Aligned: 115 rows
Threshold Bit Score: 92.6685
Threshold Setting Gi: 116494067
Created: 28-Feb-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           #### #                                                  #                   
1W0H_A       12 ICIIDFEATCeegnp----pefvhEIIEFPVVLLNTHTle-iEDTFQQYVRPEintQLSDFCISL--TGITQDQVDRADT 84  human
YP_518029    16 FLVVDFEFSVpqsygk--prawfpEIIEVGATVLDSNGklalDKTFNAFVKPRfwpRLAEESYGI--TGIRQEDVDQGIL 91  Desulfitobacteri...
AAB61982      6 LLIIDFEFTMpdgkys--pqnffpEIIEAGIVKSIDDEv---VETFSSYVRPKkfpKLTKRCKSF--LKITQKQVDEGMR 78  Bacillus subtili...
ZP_02205988  11 YVFVDFEMNLidsehkqekkicksEIIEIGAVKLDDEYn--eIDSFKSYVKPAy-gNMASRIIKL--TGITDEMLETAPS 85  Coprococcus euta...
YP_878592    18 LLAIDFEFITtkivenk-akkyfqEIIEVGVIFKSESL----TQKYSKVIKPRyflNSKDKSKSIygGRFSYDDIKNGEE 92  Clostridium novy...
ZP_02728128   4 KLFIDFEFNIlddnknkpkeyngaELISIGGVLVDNEFn--tIDNYYSLVKPKynkILSNKCKNL--TKLNQLDINNAPN 79  Clostridium diff...
ZP_02729457   3 RVYIDFEMNMpnsksk--ravfnsDIIAIGAVMYDEKTk--nIDKFKSLIRPVsneELYPHIQEL--THISSEELKSAPS 76  Clostridium diff...
ZP_01576295   2 LIFVDFEATCwdknek--ykqphaEIIEIGAVATGKDLk--eIGKFSAVVKPTiepLLSDYCKEL--TGLSQTDVENGIE 75  Clostridium cell...
ZP_02207821  12 HIVVDLEMNWldfkyenerkistnEVIEIGAVTMNEKFe--eIGAFKMYVKPQfneKVVKKVEKL--TGITMDKLQDSPV 87  Coprococcus euta...
ZP_02210280   3 KIYMDFEMNMnntknk--regfkaDLIAIGAIKYDTKTk--kIEKFKSLIKPIltkTVYPHIEEL--THITTEDLENAPT 76  Clostridium bart...
Feature 1                                  #####        #                                       
1W0H_A       85 FPQVLKKVIDWXKLkelgtkykYSLLTDGSWD-----XSKFLNIQCQLSrlk-------ypPFAKKWINIRKSYGNFYkv 152 human
YP_518029    92 LEEAIQHLQKLAHPe-------AYVVAWGDAD-----RKILGNVCEKYGlk--------ypFVWENYIDLAEQYKHYRsl 151 Desulfitobacteri...
AAB61982     79 FEDFIRKLNELDPEk------nSTIITWGNMD-----MKVLKQNCMFNHip---------fPFKGEMRDLSLEYKNFFgd 138 Bacillus subtili...
ZP_02205988  86 YEDAVGRFIEWCADa-------DVIYAWSEND-----LRQLRGETRLKQythp-----eleRVFAKWQDFQKEYARMIgt 148 Coprococcus euta...
YP_878592    93 INKVFLELQQLYTPk------eTLWISWGKAE-----YDILKTVCNKYGin--------lpFLKEDYLDLSLEFKEFYgi 153 Clostridium novy...
ZP_02728128  80 LLYVMDDFYKWFCK--------FDDVTIYNWGdf--dITGLLTSFRVYKytg------kclELFNMMIDIQPFISQHIty 143 Clostridium diff...
ZP_02729457  77 YEEVMRKFKKWLGIfs----diKGIYTFGNLDltcfnNTDMRSAKKNNHprfvnnirdlfvDIKEKYINCGMRCMNYI-- 150 Clostridium diff...
ZP_01576295  76 FNRAIDLFYQWIIQyq-----dTDTPILYTWG-----NYDGFLLKRNLRrhrnknkdflkiIQKGGVANLQEKFLEFTkl 145 Clostridium cell...
ZP_02207821  88 FADAFDAFIKWCISf-------GEEVVMYGWS-----DSDELQLRREMKlknipw-kedvdKVFRQWHDFQKEFSDLVgi 154 Coprococcus euta...
ZP_02210280  77 YESVMRSFKHWLGDfn----eiDGIYTFGNLDltcfkNTDRISSQKNNHprf-------lnNIQNFFVDIKEKYLEYGvk 145 Clostridium bart...
Feature 1                                 #    #            
1W0H_A      153 pr----sqtKLTIXLEKLGXdydgrpHCGLDDSKNIARIAVRXL 192 human
YP_518029   152 d-----hliSLKKAIEENSIeqigilHSALDDAINAAQVMARMM 190 Desulfitobacterium hafniense Y51
AAB61982    139 r-----tltGLWKAAEEYGDsgtgthHKAHDDALTAYKLFKLVE 177 Bacillus subtilis subsp. subtilis str. 168
ZP_02205988 149 s-----rriSLSDAVFYLGEnfqgaeHDALWDARNTAEVFKLSK 187 Coprococcus eutactus ATCC 27759
YP_878592   154 g-----qnaSLDKALNYLNIpii-nrHYAIFDAQALMSIIHKMF 191 Clostridium novyi NT
ZP_02728128 144 nnrilskqiSLLNMKKIFSVegd-ikHNSLSDAVDLMNVCKCFF 186 Clostridium difficile QCD-37x79
ZP_02729457 151 ---------SLKNLLEFVNLefsgdaHDPLNDAYNLFILDEAIT 185 Clostridium difficile QCD-37x79
ZP_01576295 146 pc----sscSLTKALQIIGEdyrgikHRSINDAANMIKLYKYLG 185 Clostridium cellulolyticum H10
ZP_02207821 155 g-----riiTLSQAVDYAGIdfagqkHDACCDARNTAYLLALTK 193 Coprococcus eutactus ATCC 27759
ZP_02210280 146 cm----nyiSLTHLLELSNMkfsgdaHDPLYDAYNLHILDEILE 185 Clostridium bartlettii DSM 16795

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